Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Cloning and Initial Analysis of Porcine MPDU1 Gene

  • Yang, J. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Yu, M. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Liu, B. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Fan, B. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Zhu, M. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Xiong, T. (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University) ;
  • Li, Kui (Lab of Molecular Biology and Animal Breeding, School of Animal Science and Technology Huazhong Agricultural University)
  • Received : 2004.09.26
  • Accepted : 2005.04.01
  • Published : 2005.09.01
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Abstract

Mannose-P-dolichol utilization defect 1 (MPDU1) gene is required for utilization of the mannose donor MPD in synthesis of both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols (GPI) which are important for functions such as protein folding and membrane anchoring. The full length cDNA of the porcine MPDU1 was determined by in silico cloning and rapid amplification of cDNA ends (RACE). The deduced amino acid showed 91% identity to the corresponding human sequence with five predicted transmembrane regions. RT-PCR was performed to detect its expression pattern in five tissues and results showed that it is expressed ubiquitously among the tissues checked. A single nucleotide substitution resulting in the amino acid change (137 Tyr-137 His) was detected within exon 5. Allele frequencies in six pig breeds showed distinctive differences between those Chinese indigenous pigs breeds and European pigs. Using the pig/rodent somatic cell hybrid panel (SCHP), we mapped the porcine MPDU1 gene to SSC12, which is consistent with the comparative mapping result as conservative syntenic groups presented between human chromosome 17 and pig chromosome 12.

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References

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