• Biomolecules & Therapeutics
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• v.21 no.6
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• pp.487-492
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• 2013

Cytochrome P450 4A11 (CYP4A11) is a fatty acid hydroxylase enzyme expressed in human liver. It catalyzes not only the hydroxylation of saturated and unsaturated fatty acids, but the conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), a regulator of blood pressure. In this study, we performed a directed evolution analysis of CYP4A11 using the luminogenic assay system. A random mutant library of CYP4A11, in which mutations were made throughout the entire coding region, was screened with luciferase activity to detect the demethylation of luciferin-4A (2-[6-methoxyquinolin-2-yl]-4,5-dihydrothiazole-4-carboxylic acid) of CYP4A11 mutants in Escherichia coli. Consecutive rounds of random mutagenesis and screening yielded three improved CYP4A11 mutants, CP2600 (A24T/T263A), CP2601 (T263A), and CP2616 (A24T/T263A/V430E) with ~3-fold increase in whole cells and >10-fold increase in purified proteins on the luminescence assay. However, the steady state kinetic analysis for lauric acid hydroxylation showed the significant reductions in enzymatic activities in all three mutants. A mutant, CP2600, showed a 51% decrease in catalytic efficiency ($k_{cat}/K_m$) for lauric acid hydroxylation mainly due to an increase in $K_m$. CP2601 and CP2616 showed much greater reductions (>75%) in the catalytic efficiency due to both a decrease in $k_{cat}$ and an increase in Km. These decreased catalytic activities of CP2601 and CP2616 can be partially attributed to the changes in substrate affinities. These results suggest that the enzymatic activities of CYP4A11 mutants selected from directed evolution using a luminogenic P450 substrate may not demonstrate a direct correlation with the hydroxylation activities of lauric acid.

• Journal of Fisheries and Marine Sciences Education
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• v.27 no.3
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• pp.735-746
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• 2015

Education is the study of work-related education and human aspects. Therefore, it should be understood first fully human. Education is not possible to leave the understanding of human nature. In particular, the history of educational development in China can be grasped by understanding the Humanism Theory. Therefore, to study the evolution of China Humanism Theory to find the historical footprints of Chinese education. Humanism Theory will be able to recognize the impact on educational theory development in China. In conclusion, character education is to increase the economic and social status is revealed and influenced. That the Humanism can grow through training and education is a common opinion of many scholars.

• Proceedings of the KIEE Conference
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• summer
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• pp.1409-1411
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• 2004

[$80\;\sim\;90\%$ of Information that human masters from outside are image information which depend on sight. So, in case machine takes the place of human's function, role of image processing is very important. However, because human's sight function is formed through evolution and studying for a long time, substituting it for computer attend with unexpected difficulty. In railroad, there are many field which the application of automation system is expected by using computer instead of safety watch that depend on usual human sight, macrography. But, there are many research field and conditions in the railroad when various outside conditions are considered, comparing with another field. This study analyses the recent image processing technology and reviews the task of image processing technology and its direction.]

• Genomics & Informatics
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• v.5 no.4
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• pp.179-187
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• 2007

An increasing number of primate genomes are being sequenced. A direct comparison of repeat elements in human genes and their corresponding chimpanzee orthologs will not only give information on their evolution, but also shed light on the major evolutionary events that shaped our species. We have developed REPEATOME to enable visualization and subsequent comparisons of human and chimpanzee repeat elements. REPEATOME (http://www.repeatome.org/) provides easy access to a complete repeat element map of the human genome, as well as repeat element-associated information. It provides a convenient and effective way to access the repeat elements within or spanning the functional regions in human and chimpanzee genome sequences. REPEATOME includes information to compare repeat elements and gene structures of human genes and their counterparts in chimpanzee. This database can be accessed using comparative search options such as intersection, union, and difference to find lineage-specific or common repeat elements. REPEATOME allows researchers to perform visualization and comparative analysis of repeat elements in human and chimpanzee.

• Journal of Ecology and Environment
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• v.39 no.1
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• pp.11-16
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• 2016

Declines in amphibian populations are occurring worldwide, and have been attributed to many factors, including anthropogenic environmental changes. One of the ramifications of such declines is abnormalities in many amphibian species. A strong association has been detected between human activities and abnormalities in amphibian populations, but studies on this association are largely focused on lentic species. In this study, it was analyzed whether the degree of local human activity was associated with the rate of abnormalities in Bombina orientalis which inhabited lotic environments. We found that the proportions of abnormalities in wild populations of B. orientalis increased, when i) the closest human land use was located within 100 m from the frogs' habitat, and ii) the proportion of human land use within a 300-m radius was high. Our findings suggest that human activity has a negative impact on the fitness of nearby amphibian populations, and that wild populations very close to human-induced disturbance are affected.

• Cartoon and Animation Studies
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• s.18
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• pp.37-56
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• 2010

This paper focalizes on the 'evolution' of monster animation and . Actually, Many characters' 'Evolution' is a very powerful convention of Japanese genre animation. Lots of young people feel a huge pleasure about character's evolution. It is a fantastic spectacle to boys and girls. The first characteristic point of characters' evolution is growing-up body to an adult. For example, magical girls genre shows a woman's sexy body. This scene gives a sexual fantasy to young girl spectators, a voyeuristic visual pleasure to young boy spectators. The second point is that a transforming-body gains some super power or magical power. The third point is that a great many characters is in one text. In , characters' numbers go on increasing through 6 ways-hybrid type, mutant-reiteration type, metamorphosis type, simple-grownup type, narrative type, multiple evolution type. The evolution of has to pay attention to only one transforming way. Most Digimon characters are becoming to a human-weapons cyborg. In my opinion, this is very dangerous phenomenon with a political ideology.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.4A
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• pp.399-414
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• 2004

In this paper, an evolution of software reliability engineering in a large-scale software project is summarized. The considered software consists of many components, called functional blocks in software of switching system. These functional blocks are served as the unit of coding and test, and the software is continuously updated by adding new functional blocks. We are mainly concerned with the analysis of the effects of these software components in software reliability and reliability evolution. We analyze the static characteristics of the software related to software reliability using collected failure data during system test. We also discussed a pattern which represents a local and global growth of the software reliability as version evolves. To find the pattern of system software, we apply the S-shaped model to a collection of failure data sets of each evolutionary version and the Goel-Okumoto(G-O) model to a grouped overall failure data set. We expect this pattern analysis will be helpful to plan and manage necessary human/resources fur a new similar software project which is developed under the same developing circumstances by estimating the total software failures with respect to its size and time.

• Journal of The Korean Astronomical Society
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• v.36 no.3
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• pp.177-187
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• 2003

Active galactic nuclei (AGNs) are distant, powerful sources of radiation over the entire electromagnetic spectrum, from radio waves to gamma-rays. There is much evidence that they are driven by gravitational accretion of stars, dust, and gas, onto central massive black holes (MBHs) imprisoning anywhere from $\~$1 to $\~$10,000 million solar masses; such objects may naturally form in the centers of galaxies during their normal dynamical evolution. A small fraction of AGNs, of the radio-loud type (RLAGNs), are somehow able to generate powerful synchrotron-emitting structures (cores, jets, lobes) with sizes ranging from pc to Mpc. A brief summary of AGN observations and theories is given, with an emphasis on RLAGNs. Preliminary results from the imaging of 10000 extragalactic radio sources observed in the MITVLA snapshot survey, and from a new analytic theory of the time-variable power output from Kerr black hole magnetospheres, are presented. To better understand the complex physical processes within the central engines of AGNs, it is important to confront the observations with theories, from the viewpoint of analyzing the time-variable behaviours of AGNs - which have been recorded over both 'short' human ($10^0-10^9\;s$) and 'long' cosmic ($10^{13} - 10^{17}\;s$) timescales. Some key ingredients of a basic mathematical formalism are outlined, which may help in building detailed Monte-Carlo models of evolving AGN populations; such numerical calculations should be potentially important tools for useful interpretation of the large amounts of statistical data now publicly available for both AGNs and RLAGNs.

• Molecules and Cells
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• v.22 no.3
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• pp.360-363
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• 2006

Human endogenous retroviruses (HERVs) contribute to various kinds of genomic instability via rearrangement and retrotransposition events. In the present study the formation of a new human-specific solo-LTR belonging to the HERV-H family (AP001667; chromosome 21q21) was detected by a comparative analysis of human chromosome 21 and chimpanzee chromosome 22. The solo-LTR was formed as a result of an equal homologous recombination excision event. Several evolutionary processes have occurred at this locus during primate evolution, indicating that mammalian-wide interspersed repeat (MIR) and full-length HERV-H elements integrated into hominoid genomes after the divergence of Old World monkeys and hominoids, and that the solo-LTR element was created by recombination excision of the HERV-H only in the human genome.

• The Korean Journal of Mycology
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• v.46 no.3
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• pp.205-211
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• 2018

Fungal endophytes are defined as fungi inhabiting plant tissues, causing no apparent disease. As the agricultural and ecological importance of endophytes has increased, many studies have been performed on various aspects, ranging from basic identification, taxonomy, and evolution to secondary metabolites with potential for human use. In the Korean Peninsula, conifers are the most commonly found evergreen trees, with approximately 30 to 60 reported taxa. Studies on the effect of conifer-associated endophytes on the host plants are required for the preservation and conservation of coniferous forests which decline by climate change and deforestation. This review summarizes the diversity of endophytic fungi in coniferous trees of Korea and their relationship with host plants.