• 한국정보전자통신기술학회논문지
• /
• 제2권2호
• /
• pp.3-8
• /
• 2009

척추 및 보행질환 등에서 재활과 교정을 위하여 정형외과 및 가정 등에서는 견인운동치료기를 주로 사용하고 있다. 하지만 견인운동치료기를 사용함에 있어 간혹 무리하게 사용함에 따라 인체에 문제점이 발생하고 있다. 지속적인 견인력 작용을 이용한 치료방법에 견인운동치료기를 이용하는데, 이 때 작용하는 견인력을 측정하여 운동 시간을 조절할 필요가 있다. 그러나 현재 출시되고 있는 제품에는 견인력을 측정하는 센서가 대부분이 장착되어 있지 않고 있다. 그래서 스트레인 게이지를 이용한 견인측정센서, 출력신호로서의 변환을 위한 증폭기 및 성능 검증을 위하여 측정용 실험 장치를 설계 제작하고, 이를 이용하여 견인측정센서를 실험 하였다. 견인 부하에 따른 견인측정센서의 전기적 반응치를 측정하고 분석 결과, 캘리브레이션을 통하여 센서는 선형적인 출력을 보였고 환자의 움직임 여부에 관계없이 일정하게 견인측정센서의 반응이 나타남을 알 수가 있었다. 정적인 상태에서의 실험에서 최대 에러율이 약 1%이내이고, 동적인 실험에서 평균 에러율이 약 0.7%로 나타났다. 온도 변화에 따른 견인측정센서의 최대 출력치 변화량(output variation)이 약 0.3%이므로 견인 측정용 센서로 사용 가능하다고 판단된다.

• 대한수의학회지
• /
• 제51권2호
• /
• pp.117-122
• /
• 2011

Bovine tuberculosis (bTB), caused primarily by Mycobacterium bovis, continues to exert an economic loss, even in countries with active control measures, and is one of zoonotic diseases enable to be transmitted to human. The control and eradication of bTB are mainly based on a test and slaughter policy and/or abattoir surveillance. Various factors including limitation of diagnostic tests have been considered as major constraints to eradication. Single intradermal test (SIT) is the official diagnostic test. New diagnostic methods are needed to be developed, because of limitations of the test. In the present study SIT was compared with single intradermal comparative cervical test (SICCT) and interferon (IFN)-${\gamma}$ assay. There was very low correlation between SIT and SICCT. However, high correlation was shown between SIT and IFN-${\gamma}$ assay while no correlation was observed between SICCT and IFN-${\gamma}$ assay. Therefore, our results suggest the possibility of replacement of SIT with IFN-${\gamma}$ assay for the diagnosis of bovine tuberculosis.

• 한국안광학회지
• /
• 제13권3호
• /
• pp.103-109
• /
• 2008

목적: 본 논문에서는 활성산소(reactive oxygen species, ROS)와 활성질소(reactive oxygen species, RNS)생성의 결과 초래되는 산화스트레스(oxidative stress)와 안질환과의 관계, 특히, 백내장발생과의 관련성 연구에 대한 고찰과, 안구의 기능이상에 있어 산화스트레스의 매개체(mediator)로서 과산화지질(lipid peroxide)의 역할에 대해 논의하고자 한다. 방법: 산화스트레스는 단백질 산화, DNA 파괴, 세포사(apoptosis), 지질과산화(lipid peroxidation) 등의 다양한 세포손상을 나타낸다. 이러한 손상은 많은 질병의 발생과 관련되어 있다. 백내장 발생의 주요한 원인중의 하나가 안구조직이 일정하고 지속적으로 산화스트레스의 환경에 노출되는 것으로 알려져 있다. 따라서 산화스트레스의 안구기능이상에 대한 역할을 조사하였다. 결과: 수정체는 자외선에의 만성적인 노출과 세포대사과정에서 필수불가결하게 생성되는 활성산소에 의해 끊임없이 공격을 받는다. 과도하게 생성된 활성산소에 의한 수정체 단백질의 분해(degradation), 산화(oxidation), 가교형성(crosslinking), 응집(aggregation) 등은 백내장발생에 있어 중요한 요인으로 사료된다. 결론: 산화스트레스와 체내의 산화/항산화 불균형이 과도한 활성산소를 생성하게 되고 결국, 안구의 기능이상을 일으킨다고 할 수 있다. 이러한 결과들에도 불구하고, 산화스트레스와 안구이상과의 관계를 더욱 정확하게 설명할 수 있는 분자기전에 대한 정보는 아직 부족한 상태이며, 더욱 많은 연구가 필요하다.

• 대한한방내과학회지
• /
• 제27권3호
• /
• pp.639-652
• /
• 2006

Objective : The etiology of chronic prostatitis is likely multifactorial, resulting from either a cascade of events after an initiating factor or from a variety of etiologic mechanisms. There is substantiating evidence to support the role of the inflammatory responses in its pathogenesis, and the clinical value in the evaluation of therapeutic efficacy. Forsythiae Frucus has been traditionally used in treatment of inflammatory diseases, including of prostatitis and urinary tract inflammation. In this study, we investigated the effects of Forsythiae Frucus on inflammatory cytokines and cyto-pathological alternation in the rat model of chronic non-bacterial prostatitis induced by castration and $17{\beta}$-estradiol treatment. Methods : Two-month-old rats were treated with $17{\beta}$-estradiol after castration for induction of experimental non-bacterial prostatitis. which is similar to human chronic prostatitis in histopathological profiles. Forsythiae Frucus as an experimental specimen, and testosterone as a positive control, were administered orally. The prostates were evaluated by histopathologlcal parameters including the epithelial score and epithelio-stromal ratio for glandular damage. and the expression of inflammatory cytokine genes including interleukin (IL)-$1{\beta}$, IL-5, IL-12, tumor necrosis factor (TNF)-$\alpha$. eotaxin, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2(cox-2). Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation. the rats treated with Forsythiae Frucus showed a diminished range of tissue damage. Epithelial score was improved in the Forsythiae Frucus group over that of the control (P<0.05). The epithelia-stromal ratio was lower in the Forsythiae Frucus group when compared to that of the control (P<0.05). In the reverse transcription-polymerase chain reaction (RT-PCR) of inflammatory cytosine genes. Forsythiae Frucus inhibited the expression of IL-$1{\beta}$, TNF-$\alpha$, iNOS, cox-2 genes, while it modulated the expression of IL-5, which is an anti-inflammatory cytokine. Conclusions : These findings suggest that Forsythiae Frucus may protect the glandular epithelial cells and also inhibit stromal proliferation in association with the immune modulation including the suppression of inflammatory cytokines and increase of anti-inflammatory cytokines. From theses results. we suggest that Forsythiae Frucus could be a useful remedy agents for treating chronic non-bacterial prostatitis.

• 동의생리병리학회지
• /
• 제20권4호
• /
• pp.936-941
• /
• 2006

To prevent human body injury from oxidative stress, antioxidants are very important and many research about antioxidants are generally being conducted. Hydrogen peroxide$(H_20_2)$ that is one of vitality oxygen species has been seen that cause various diseases, DNA damage and gene change. We have already known that the inhibition effect of Zizania latifolia Radix, Rhizoma on apoptosis induced by $H_2O_2$ in Neuro2A cell. And the purpose of this study was that we made a comparative study on the inhibition effect of apoptosis in Neuro2A cell on the region of Zizania latifolia(Radix, Rhizoma, Herba). Neuro2A cells were cultivated in RPMI(GibcoBRL) with 5% FBS and treated with $H_2O_2$ and Zizania latifolia(Radix, Rhizoma, Herba). Separately we measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined by using western blot. The results obtained were as Follows: The cell viability in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment (60ug/m1<) decreased significantly compared with that of none treatment(p<0.001). Zizania latifolia Radix increased cell viability was most effective of three regions. But we had no significant difference among three regions. All of Zizania latifolia (Radix, Rhizoma, Herba) increased cell viability about twice as much as that being injury by $H_2O_2$,(Zizania Latifolia (Radix, nhizoma, Herba) 20ug/m1, $H_2O_2$ 200uM, p<0.001). DNA fragmentation developed by $H_2O_2$, but was not developed in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by $H_2O_2$ but were not activated in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. P53, P2l and Bax activated by $H_2O_2$, and Bcl-2 got into inactivation. But the opposite results appeared in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. In conclusion, these results suggest that all of Zizania latifolia (Radix, Rhizoma, Herba) inhibit the development of DNA fragmentation and apoptosis by $H_2O_2$and the antioridant action of all of Zizania latifolia (Radix, Rhizoma, Herba) is effective.

• Biomolecules & Therapeutics
• /
• 제28권5호
• /
• pp.456-464
• /
• 2020

Mast cells (MCs) are systemically distributed and secrete several allergic mediators such as histamine and leukotrienes to cause type I hypersensitivity. Dasatinib is a type of anti-cancer agent and it has also been reported to inhibit human basophils. However, dasatinib has not been reported for its inhibitory effects on MCs or type I hypersensitivity in mice. In this study, we examined the inhibitory effect of dasatinib on MCs and MC-mediated allergic response in vitro and in vivo. In vitro, dasatinib inhibited the degranulation of MCs by antigen stimulation in a dose-dependent manner (IC₅₀, ~34 nM for RBL-2H3 cells; ~52 nM for BMMCs) without any cytotoxicity. It also suppressed the secretion of inflammatory cytokines IL-4 and TNF-α by antigen stimulation. Furthermore, dasatinib inhibited MC-mediated passive cutaneous anaphylaxis (PCA) in mice (ED₅₀, ~29 mg/kg). Notably, dasatinib significantly suppressed the degranulation of MCs in the ear tissue. As the mechanism of its effect, dasatinib inhibited the activation of Syk and Syk-mediated downstream signaling proteins, LAT, PLCγ1, and three typical MAP kinases (Erk1/2, JNK, and p38), which are essential for the activation of MCs. Interestingly, in vitro tyrosine kinase assay, dasatinib directly inhibited the activities of Lyn and Fyn, the upstream tyrosine kinases of Syk in MCs. Taken together, dasatinib suppresses MCs and PCA in vitro and in vivo through the inhibition of Lyn and Fyn Src-family kinases. Therefore, we suggest the possibility of repositioning the anti-cancer drug dasatinib as a treatment for various MC-mediated type I hypersensitive diseases.

• Biomolecules & Therapeutics
• /
• 제28권5호
• /
• pp.437-442
• /
• 2020

Activation of the NLRP3 inflammasome is critical for host defense as well as the progression of inflammatory diseases through the production of the proinflammatory cytokine IL-1β, which is cleaved by active caspase-1. It has been reported that overactivation of the NLRP3 inflammasome contributes to the development and pathology of acne vulgaris. Therefore, inhibiting activation of the NLRP3 inflammasome may provide a new therapeutic strategy for acne vulgaris. In this study, we investigated whether auranofin, an anti-rheumatoid arthritis agent, inhibited NLRP3 inflammasome activation, thereby effectively treating acne vulgaris. Auranofin suppressed NLRP3 inflammasome activation induced by Propionibacterium acnes, reducing the production of IL-1β in primary mouse macrophages and human sebocytes. In a P. acnes-induced acne mouse model, injection of P. acnes into the ears of mice induced acne symptoms such as redness, swelling, and neutrophil infiltration. Topical application of auranofin (0.5 or 1%) to mouse ears significantly reduced the inflammatory symptoms of acne vulgaris induced by P. acnes injection. Topical application of auranofin led to the downregulation of the NLRP3 inflammasome activated by P. acnes in mouse ear skin. These results show that auranofin inhibits the NLRP3 inflammasome, the activation of which is associated with acne symptoms. The results further suggest that topical application of auranofin could be a new therapeutic strategy for treating acne vulgaris by targeting the NLRP3 inflammasome.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• 제18권1호
• /
• pp.48-54
• /
• 2015

Purpose: Metabolic liver disease (MLD) often progresses to life-threatening conditions. This study intends to describe the outcomes of liver transplantation (LTx) for MLD at a living donor-dominant transplantation center where potentially heterozygous carrier grafts are employed. Methods: We retrospectively evaluated the medical records of 54 patients with MLD who underwent LTx between November 1995 and February 2012 at Asan Medical Center in Seoul, Korea. The cumulative graft and patient survival rates were analyzed according to patient age, and living or deceased donor LTx. Recurrence of the original disease was also investigated. Results: The post-transplant cumulative patient survival rates at one, five, and 10 years were 90.7%, 87.5% and 87.5%, and the graft survival rates were 88.8%, 85.5%, and 85.5%, respectively. There were no differences in the patient survival rates according to the recipient age, human leukocyte antigen matching, and living or deceased donor LTx. There were also no differences in the patient survival rates between the MLD and the non-MLD groups for children. Recurrence of the original metabolic disease was not observed in any patient during the follow-up period. Conclusion: Our results suggest that the living donor-dominant transplantation program is well-tolerated in MLD without recurrence of the original MLD using all types of transplantation.

• Childhood Kidney Diseases
• /
• 제17권2호
• /
• pp.122-126
• /
• 2013

8개월 여아가 지속적인 발열 및 다발성 요로 결석으로 입원하여 시행한 신장 초음파에서 오른쪽 신우 및 요관, 왼쪽 수질 부위의 다발성 요로 결석이 보였고, 자연 배출된 1 cm 크기의 결석이 100% 시스틴석으로 확인되었다. 소변 검사에서 시스틴을 비롯한 이염기 아미노산의 상승과 유전자 검사에서 SLC3A1의 c.1820del (p.L607fs)의 동형접합자로 확인되어 시스틴뇨증으로 진단되었다. 충분한 수분 섭취와 소변 알칼리화, 동물성 단백질 제한에도 불구하고 결석의 수와 크기가 증가하여 현재는 tiopronin으로 치료 중이다.

• 보건의료산업학회지
• /
• 제3권1호
• /
• pp.14-24
• /
• 2009

This study aims at deriving any useful information necessary to strengthen the competitiveness for growth through empirical analyses on general hospital located in a province in order to countermeasure the opening and competition of medical markets. The characteristics of user were identified on the basis of disease groups under KCD in the research method. In addition, the analysis on the expenses of diagnosis and treatment was divided into the treatment progress and degree of hospital resource utilization And the regression was carried out to identify the impacts of characteristics of inpatient users on the degree of hospital resource utilization. As a result of major research, the inpatient users of the general hospital located in the provincial area in consideration of inpatient users were formed around the inpatient disease groups representative for Korea(diseases of the respiratory system, injury and poisoning & certain other consequences of external causes). And it was understood that most of residents within a distance of 40 minute by the public transportation were using. And mostly were under the age of 9 or over 60, and the provision of medical features such as the degree of consultation and operational functions were inadequate. When we classify inpatient treatment cost for each resource application as the medical cost being the center of patient care function, the equipment and human resource application sector are constituted over half. Accordingly, the following suggestions are made as plans to strengthen the competitiveness for the growth of general hospitals located in the provincial areas on the basis of analytical results. First, it is necessary to have the characterization matching to the age and disease groups with a high frequency. Second, it is necessary to increase the degree of hospital resource utilization according to the characterization. Third, it is necessary to concentrate on public relations. The above suggestion, as a method for securing image improvement and competitive power as a general hospital, and through expansion of social function that a regional general hospital needs to secure not only as an individual institution but also as a general hospital, it can be seen that a general improvement of image as a regional general hospital is possible.