• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.80.2-81
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• 2003

Since hot peppers (Capsicum annuum L.) are getting reputation as an important source of vitamins, medicine and many other areas, consumption and cultivation is being increased in the world. In spite of this usefulness, so little attention has been given to the hot pepper plants. To date, less than 500 nucleotide sequences including redundancy has been identified in NCBI database. Therefore we started to EST sequencing project for initial characterization of the genome, because of the large genome size of hot pepper (2.7 3.3 ${\times}$ 109 bp), To date, a set of 10,000 non-redundant genes were identified by EST sequencing for microarray-based gene expression studies. At present, cDNA microarrays containing 4,685 unigene clones are used for hybridization labeled targets derived from pathogen infected and uninoculated leaf tissues. Monitoring of gene expression profiles of hot pepper interactions with soybean pustule pathogen (Xag；Xanthomonas axonopodis pv. glycine) will be presented.

• 식물조직배양학회지
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• 제24권1호
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• pp.37-41
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• 1997

고추의 형질전환율을 높이기 위하여 우선적으로 효율적인 재분화조건을 구명하였다. 고추의 하배축과 자엽 모두 2mg/L zeatin과 0.1 mg/L NAA ( I )에서 51%, 1.0 mg/L BAP와 10.0 mg/L IBA ( II)는 45.1%의 재분화율을 보였으며, 두 배지에 5 $\mu$M AgNO$_3$을 첨가하였을 때 I의 배지에서 보다 건강한 식물체를, II의 배지에서는 재분화율이 약 8%로 증가함을 보였다. 따라서 II의 배지 조건은 가격이 비싼 zeatin의 효과를 대치할 수 있었다. 이렇게 얻어진 효율적인 재분화배지에 고추의 하배축과 자엽을 ADA와 NPT II 유전자를 함유한 Agrobacterium tumefaciens pDY183을 이용하여 형질전환을 유도하여, kanamycin 100 mg/L에서 선발하여 성공적으로 형질전환체를 얻었다. 식물체내로의 ADA와 NPT II 유전자의 도입은 PCR을 이용하여 확인하였으며, Northern blot에 의하여 ADA 유전자의 전사여부를 확인하였다. ADA 효소의 활성도는 spectrophotometer를 이용하여 측정하여 본 결과 고추세포내에서 정상적으로 발현하였으므로 동물유전자인 ADA가 식물체 형질전환시 표시 유전자로서의 사용가능성이 확인되었다.

• Molecules and Cells
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• 제20권2호
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• pp.247-255
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• 2005

Three different catalase cDNA clones (CaCat1, CaCat2, and CaCat3) were isolated from hot pepper (Capsicum annuum L.), and their expression patterns were analyzed at the levels of mRNA and enzyme activity. Northern hybridization showed that the three catalase genes were differentially expressed in various organs, and that expression of CaCat1 and CaCat2 was regulated differently by the circadian rhythm. In situ hybridization revealed different spatial distributions of CaCat1 and CaCat2 transcripts in leaf and stem. In response to wounding and paraquat treatment, CaCat1 mRNA increased at 4-12 h in both paraquat-treated and systemic leaves. In contrast, wounding had no significant effect on expression of the catalase genes. The increase of catalase activity in the paraquat-treated and systemic leaves paralleled that of CaCat1 mRNA, but did not match that of CaCat1 mRNA in paraquat-treated stems. Our results suggest that CaCat1 may play a role in responses to environmental stresses.

• The Plant Pathology Journal
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• 제17권6호
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• pp.391.2-391
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• 2001

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.181.2-181
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• 2003

No Abstract, See Full Text

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.89-94
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• 2001

In vitro plant regeneration of inbred breeding line of hot pepper (Capsicum annuum L.) was established using leaf and petiole segments as explants. About 28 days old plants were excised and cultured on MS medium supplemented with TDZ and NAA or in combination with Zeatin. In all of the media compositions tested, combination of TDZ 0.5 mg/L, Zeatin 0.5 mg/L, and NAA 0.1 mg/L was found to be the best medium for shoot bud initiation. Young petiole was the most appropriate explant type for the plant regeneration as well as genetic transformation in hot pepper. In this study, HpMADS1 gene isolated from hot pepper was introduced using Agrobacterium-mediated transformation system. Based on the analysis of Southern blot and RT-PCR, HpMADS1 gene was integrated in the hot pepper genome. It has been known that floral organ development is controlled by a group of regulatory factors containing the MADS domain. Morphological characteristics in these transgenic plants, especially flowering habit, however, were not significantly altered, indicating this MADS gene, HpMADS1 may be non-functional in this case.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.184-192
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• 2009

The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.472-482
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• 2010

For the massive screening of the genes related to oxidative stress, a cDNA library was constructed from hot pepper (Capsicum annuum L. cv. Nockkwang) leaves treated with methyl viologen. From this library, 1,589 cDNA clones were sequenced from their 5' ends. The sequences were clustered into 1,252 unigenes comprised of 152 contigs and 1,100 singletons. Similarity search against NCBI protein database identified 1,005 ESTs (80.3%) as Known, 197 ESTs (15.7%) as Unknown, and 50 ESTs (3.99%) as No hit. In the ESTs, oxidative stress-related genes such as ascorbate peroxidase, catalase, and osmotin precursor were highly expressed. The cDNA microarray containing 1,252 unigenes was constructed and used to analyze their expression upon methyl viologen treatment. Analyses of the hybridization revealed that various stress-related genes such as peroxidase, tyrosine aminotransferase, and omega-6 fatty acid desaturase, were induced and some metabolism related genes such as aldolase and ketol-acid reductoisomerase, were repressed by methyl viologen treatment, respectively. The information from this study will be used for further study on the functional roles of oxidative stress-related genes and signaling network of oxidative stress in hot pepper.

• 생물환경조절학회지
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• 제18권4호
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• pp.393-400
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• 2009

적심 및 rootone 처리가 고추 10품종의 발근력에 미치는 영향을 조사한 결과, 모두 정상적으로 발근되었으나 품종간에 큰 차이를 보였다. 즉 'Gukbo'와 'Chungyang' 등은 발근력이 낮은 반면, 대목 이용 가능성이 높은 'Geumsang' 등은 매우 높았다. 발근촉진제 rootone 처리는 모든 품종의 발근을 현저히 증가시켜 높은 실용성을 보여주었다. 또한 적심에 따른 발근 지연 현상은 rootone 처리로 거의 완벽하게 보완될 수 있었다. 선발된 대목 10품종과 접수 3품종을 적심과 IBA 단용 또는 병행처리 후 발근력을 검토한 결과, 대목 중에서는 'Tantan'이 가장 높았고, 'Konesian Hot'과 'Wonkwang 1호'도 높았다. 또한 공시된 10개 대목은 대면적에 일반재배 되는 품종인 'Manita', 'Chungyang', 'Nokkwang'보다 발근력이 높았다. IBA 처리는 발근력을 다소 향상시켰으나 rootone 처리에 비해 효과가 뚜렷하지 않았다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.262.2-262
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• 1998

No Abstract, See Full Text