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http://dx.doi.org/10.5010/JPB.2010.37.4.472

Analysis of the Oxidative Stress-Related Transcriptome from Capsicum annuum L.  

Lee, Hyoung-Seok (School of Biological Sciences, College of Natural Sciences, Seoul National University, Division of Life Sciences, Korea Polar Research Institute, KORDI)
Lee, Sang-Ho (School of Biological Sciences, College of Natural Sciences, Seoul National University, Department of Plant Biology, University of Minnesota)
Kim, Ho-Bang (School of Biological Sciences, College of Natural Sciences, Seoul National University, The Natural Science Research Institute, Myongji University)
Lee, Nam-Houn (School of Biological Sciences, College of Natural Sciences, Seoul National University)
An, Chung-Sun (School of Biological Sciences, College of Natural Sciences, Seoul National University)
Publication Information
Journal of Plant Biotechnology / v.37, no.4, 2010 , pp. 472-482 More about this Journal
Abstract
For the massive screening of the genes related to oxidative stress, a cDNA library was constructed from hot pepper (Capsicum annuum L. cv. Nockkwang) leaves treated with methyl viologen. From this library, 1,589 cDNA clones were sequenced from their 5' ends. The sequences were clustered into 1,252 unigenes comprised of 152 contigs and 1,100 singletons. Similarity search against NCBI protein database identified 1,005 ESTs (80.3%) as Known, 197 ESTs (15.7%) as Unknown, and 50 ESTs (3.99%) as No hit. In the ESTs, oxidative stress-related genes such as ascorbate peroxidase, catalase, and osmotin precursor were highly expressed. The cDNA microarray containing 1,252 unigenes was constructed and used to analyze their expression upon methyl viologen treatment. Analyses of the hybridization revealed that various stress-related genes such as peroxidase, tyrosine aminotransferase, and omega-6 fatty acid desaturase, were induced and some metabolism related genes such as aldolase and ketol-acid reductoisomerase, were repressed by methyl viologen treatment, respectively. The information from this study will be used for further study on the functional roles of oxidative stress-related genes and signaling network of oxidative stress in hot pepper.
Keywords
Capsicum annuum; EST; hot pepper; methyl viologen; microarray; oxidative stress;
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1 Pei ZM, Murata Y, Benning G, Thomine S, Klusener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406:731-734   DOI
2 Quan LJ, Zhang B, Shi WW, Li HY (2008) Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integr Plant Biol 50:2-18   DOI
3 Quinn J, Findlay VJ, Dawson K. Millar JBA, Jones N (2002) Distinct regulatory proteins control the graded transcriptional response to increasing $H_2O_2$ levels in fission yeast Schizosaccharomyces pombe. Mol Biol Cell 13:805-816   DOI
4 Supanjani KDL (2006) Hot pepper response to interactive effects of salinity and boron. Plant Soil Environ 52:227-233
5 van Loon LC, Rep M, Pieterse CM (2006) Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44:135-162   DOI
6 Wahid A, Perveen M, Gelani S, Basra SM (2007) Pretreatment of seed with $H_2O_2$ improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164:283-294   DOI
7 Wasternack C (2007) Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot (Lond) 100:681-697   DOI
8 Wolff SP (1994) Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol 233:182-189   DOI
9 Yang T, Poovaiah BW (2002) Hydrogen peroxide homeostasis activation of plant catalase by calcium/calmodulin. Plant Biol 99: 4097-4102.
10 Yuasa T, Ichimura K, Mizoguchi T, Shinozaki K (2001) Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinase. Plant Cell Physiol 42:1012-1016   DOI
11 Mahalingam R, Gomez-Buitrago A, Eckardt N, Shah N, Guevara-Garcia A, Day P, Raina R, Fedoroff NV (2003) Characterizing the stress/defense transcriptome of Arabidopsis. Genome Biol 4:R20   DOI
12 Mittler R, Berkowitz G (2001) Hydrogen peroxide, a messenger with too many roles? Redox Rep 6:69-72   DOI
13 Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490-498   DOI
14 Moscone EA, Baranyi M, Ebert I, Greilhuber J, Ehrendorfer F, Hunziker AT (2003) Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry. Ann Bot 92:21-29   DOI
15 Nishiuchi S, Liu S, Takano T (2007) Isolation and characterization of a metallothionein-1 protein in Chloris virgata Swartz that enhances stress tolerances to oxidative, salinity and carbonate stress in Saccharomyces cerevisiae. Biotechnol Lett 29:1301-1305   DOI
16 Niyogi KK (1999) Photoprotection revisited: genetic and molecular approaches. Annu Rev Plant Physiol Plant Mol Biol 50:333-359   DOI
17 Ort DR, Baker NR (2002) A photoprotective role for $O_{(2)}$ as an alternative electron sink in photosynthesis? Curr Opin Plant Biol 5:193-198   DOI
18 Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P (1999) Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science 284:654-657   DOI   ScienceOn
19 Palmeira CM, Moreno AJ, Madeira VM (1995) Mitochondrial bioenergetics is affected by the herbicide paraquat. Biochim Biophys Acta 1229:187-192   DOI
20 Park JH, Halitschke R, Kim HB, Baldwin IT, Feldmann KA, Feyereisen R (2002) A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis. Plant J 31:1-12   DOI
21 Knapp, S (2002) Tobacco to tomatoes: a phylogenetic perspective on fruit diversity in the Solanaceae. J Exp Bot 377:2001-2022
22 Kwon, SI and An CS (2003) Cloning and expression of mitochondrial MnSOD from the small radish (Raphanus sativus L.). Mol Cells 16:194-200
23 Langebartels C, Schraudner M, Heller W, Ernst D, Sandermann H (2000) Oxidative stress and defense reactions in plants exposed to air pollutants and UV-B radiation. In Inze D, Montagu MV, eds, Oxidative Stress in Plants, Harwood Academic Publishers, London, pp 105-135
24 Lee H, Cho HH, Kim I-C, Yim JH, Lee HK, Lee YK (2008) Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica. Mol Cells 25:258-264
25 Lee H, Hur CG, Oh CJ, Kim HB, Pakr SY, An CS (2004) Analysis of the root nodule-enhanced transcriptome in soybean. Mol Cells 18:53-62
26 Lee S, Yun S-C (2006) The ozone stress transcriptome of pepper (Capsicum annuum L.). Mol. Cells 21:197-205
27 Eulgem T, Rushton PJ, Robatzek S, Somssich IE (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci 5:199-206   DOI
28 Liu Y, Ren D, Pike S, Pallardy S, Gassmann W, Zhang S (2007) Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade. Plant J 51:941-954   DOI
29 Loake G, Grant M (2007) Salicylic acid in plant defence-the players and protagonists. Curr Opin Plant Biol 10:466-472   DOI
30 Mahalingam R, Fedoroff N (2001) Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays. Proc Natl Acad Sci USA 98:7420-7425   DOI
31 Foyer CH, Descourvières P, Kunert KJ (1994) Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell Environ 17:507-523   DOI
32 Gadjev I, Vanderauwera S, Gechev TS, Laloi C, Minkov IN, Shulaev V, Apel K, Inze D, Mittler R, Van Breusegem F (2006) Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis. Plant Physiol 141:436-445
33 Gao Q, Zhang L (2008) Ultraviolet-B-induced oxidative stress and antioxidant defense system responses in ascorbatedeficient vtc1 mutants of Arabidopsis thaliana. J Plant Physiol 165:138-148   DOI
34 Gechev TS, Van Breusegem F, Stone JM, Denev I, Laloi C (2006) Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays 28:1091-1101   DOI
35 Jagadeeswaran G, Saini A, Sunkar R (2009) Biotic and abiotic stress down-regulate miR398 expression in Arabidopsis. Planta 229:1009-1014   DOI
36 Grant JJ, Yun BW, Loake GJ (2000)Oxidative burst and cognate redox signalling reported by luciferase imaging: identification of a signal network that functions independently of ethylene, SA and Me-JA but is dependent on MAPKK activity. Plant J 24:569-582   DOI
37 Guo Y, Gan S (2005) Leaf senescence: signals, execution, and regulation. Curr Top Dev Biol 71:83-112   DOI
38 Hua ZM, Yang X, Fromm ME (2006) Activation of the NaCland drought-induced RD29A and RD29B promoters by constitutively active Arabidopsis MAPKK or MAPK proteins. Plant Cell Environ 29:1761-1770   DOI
39 Bosland PW, Votava EJ (1999) ‘NuMex Primavera’ jalapeno. HortScience 33:1085-1086
40 Apel k, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373-399   DOI
41 Desikan R, A-H-Mackerness S, Hancock JT, Neill SJ (2001) Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol 127:159-172   DOI
42 Desikan R, Burnett EC, Hancock JT, Neill SJ (1998) Harpin and hydrogen peroxide induce the expression of a homologue of gp91-phox in Arabidopsis thaliana suspension cultures. J Exp Bot 49:1767-1771   DOI
43 Dodge AD (1971) The mode of action of the bipyridylium herbicides, paraquat and diquat. Endeavour 30:130-135   DOI