• The Plant Pathology Journal
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• v.24 no.2
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• pp.101-111
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• 2008

The fungal genus Alternaria is comprised of many saprophytic and endophytic species, but is most well known as containing many notoriously destructive plant pathogens. There are over 4,000 Alternaria/host associations recorded in the USDA Fungal Host Index ranking the genus 10th among nearly 2,000 fungal genera based on the total number of host records. While few Alternaria species appear to have a sexual stage to their life cycles, the majority lack sexuality altogether. Many pathogenic species of Alternaria are prolific toxin producers, which facilitates their necrotrophic lifestyle. Necrotrophs must kill host cells prior to colonization, and thus these toxins are secreted to facilitate host cell death often by triggering genetically programmed apoptotic pathways or by directly causing cell damage resulting in necrosis. While many species of Alternaria produce toxins with rather broad host ranges, a closely-related group of agronomically important Alternaria species produce selective toxins with a very narrow range often to the cultivar level. Genes that code for and direct the biosynthesis of these host-specific toxins for the Alternaria alternata sensu lato lineages are often contained on small, mostly conditionally dispensable, chromosomes. Besides the role of toxins in Alternaria pathogenesis, relatively few genes and/or gene products have been identified that contribute to or are required for pathogenicity. Recently, the completion of the A. brassicicola genome sequencing project has facilitated the examination of a substantial subset of genes for their role in pathogenicity. In this review, we will highlight the role of toxins in Alternaria pathogenesis and the use of A. brassicicola as a model representative for basic virulence studies for the genus as a whole. The current status of these research efforts will be discussed.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.414-420
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• 2005

The ShortStack Micro Server enables any product that contains a microcontroller or microprocessor to quickly and inexpensively become a networked, Internet-accessible device. The ShortStack Micro Server provides a simple way to add LonWorks networking to new or existing smart devices. . It implements the LonTalk protocol and provides the physical interface with the LonWorks communication. The ShortStack host processor can be an 8, 16, or 32-bit microprocessor or microcontrollers. The ShortStack API and driver typically require about 4kbytes of program memory on the host processor and less than 200 bytes of RAM. The interface between host processor and the ShortStack Micro Server may be a Serial Communication Interface (SCI). The LonWorks control module with a high performance is developed, which is composed of the 8 bit PIC Microprocessor for host processor and the smart neuron chip for the ShortStack Micro Server. This intelligent control board is verified as proceeding the various function tests from experimental system with an boost pump and inverter driving systems. It is also confirmed that the developed control module provides stably 0-10VDC linear signal to the input signal of inverter driving system for varying the induction motor speed. Thus, the experimental results show that the fabricating intelligent board carried out very well the various functions in the wide operating ranges of boost pump system. This developed control module expect to apply to industrial fields to require the comparatively exact control and monitoring such as multi-motor driving system with inverter, variable air volume system and the boost pump water supply systems.

• Mycobiology
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• v.49 no.5
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• pp.461-468
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• 2021

The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.

• Parasites, Hosts and Diseases
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• v.57 no.2
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• pp.83-92
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• 2019

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and $H_2O_2$ levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.

• The Plant Pathology Journal
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• v.27 no.2
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• pp.128-137
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• 2011

Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at $30^{\circ}C$ and $37^{\circ}C$, average size of microsclerotia, and time to microsclerotia formation. The feathery colony phenotype was the most common (63.7%) on the chlorate selective medium and represented the chlorate sensitive phenotype of the Iranian Macrophomina phaseolina population. Meantime, inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and phenotypic characteristics. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the phenotypic and the genotypic characteristics investigated and needs more studies using neutral molecular tools to get a deeper insight into this complex species.

• The KIPS Transactions:PartC
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• v.18C no.6
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• pp.405-412
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• 2011

Our proposed mobility management scheme is based on Multicasting and HIP(Host Identity Protocol) in PMIPv6(Proxy Mobile IPv6) Networks, and allows users to handoff within and across different administrative domains. The main advantage of our scheme is to enable the inter-domain handoff of both types of nodes with a reduced signalling overhead and packet losses. Specifically, the scheme enables the interworking between host-based and network-based mobility support, by means of the interaction between PMIPv6 with Multicasting and HIP. Performance evaluations demonstrate that our scheme improves the handoff latency and packet losses compared to other global mobility management protocols.

• Journal of Information Processing Systems
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• v.9 no.4
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• pp.651-659
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• 2013

In the Host Identity Protocol (HIP), the existing host-based handover scheme tends to induce large handover delays and packet loss rates. To deal with this problem, we are proposing a network-based handover scheme for HIP in the mobile networks, in which the access routers of the mobile node will establish a handover tunnel and will perform the route optimization for data transmission. We also discuss how to extend the HIP Update message to use the proposed handover scheme. From ns-2 simulations, we can see that the proposed handover scheme can significantly reduce the handover delay and packet losses during handover, as compared to the existing handover schemes.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.12
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• pp.3100-3116
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• 2012

Reversible data hiding enables the embedding of messages in a host image without any loss of host content, which is proposed for image authentication that if the watermarked image is deemed authentic, we can revert it to the exact copy of the original image before the embedding occurred. In this paper, we present an improved histogram-based reversible data hiding scheme based on prediction and sorting. A rhombus prediction is employed to explore the prediction for histogram-based embedding. Sorting the prediction has a good influence on increasing the embedding capacity. Characteristics of the pixel difference are used to achieve large hiding capacity while keeping low distortion. The proposed scheme exploits a two-stage embedding strategy to solve the problem about communicating peak points. We also present a histogram shifting technique to prevent overflow and underflow. Performance comparisons with other existing reversible data hiding schemes are provided to demonstrate the superiority of the proposed scheme.

• The Plant Pathology Journal
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• v.31 no.3
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• pp.212-218
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• 2015

In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of $1pg/{\mu}l$ per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.23-34
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• 2018

The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis.