Browse > Article
http://dx.doi.org/10.5423/PPJ.FT.09.2017.0203

Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea  

Cho, Heejung (National Institute of Agricultural Sciences, Rural Development Administration)
Song, Eun-Sung (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Young Kee (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Seungdon (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Seon-Woo (Department of Applied Biology, Dong-A University)
Jo, Ara (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Byoung-Moo (National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Jeong-Gu (National Institute of Agricultural Sciences, Rural Development Administration)
Hwang, Ingyu (Department of Agricultural Biotechnology, Seoul National University)
Publication Information
The Plant Pathology Journal / v.34, no.1, 2018 , pp. 23-34 More about this Journal
Abstract
The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis.
Keywords
diversity; potato bacterial wilt; Ralstonia solanacearum;
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Ramesh, R., Achari, G. A. and Gaitonde, S. 2014. Genetic diversity of Ralstonia solanacearum infecting solanaceous vegetables from India reveals the existence of unknown or newer sequevars of Phylotype I strains. Eur. J. Plant Pathol. 140:543-562.   DOI
2 Safni, I., Cleenwerck, I., De Vos, P., Fegan, M., Sly, L. and Kappler, U. 2014. Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov and R. solanacearum phylotype I and III strains as Ralstonia pseudosolanacearum sp. nov. Int. J. Syst. Evol. Microbiol. 64:3087-3103.   DOI
3 Sagar, V., Jeevalatha, A., Mian, S., Chakrabarti, S. K., Gurjar, M. S., Arora, R. K., Sharma, S., Bakade, R. R. and Singh, B. P. 2014. Potato bacterial wilt in India caused by strains of phylotype I, II and IV of Ralstonia solanacearum. Eur. J. Plant Pathol. 138:51-65.   DOI
4 Buddenhagen, I., Sequeira, L. and Kelman, A. 1962. Designation of races in Pseudomonas solanacearum. Phytopathology 52:726 (Abstract).
5 Castillo, J. A. and Greenberg, J. T. 2007. Evolutionary dynamics of Ralstonia solanacearum. Appl. Environ. Microbiol. 73:1225-1238.   DOI
6 Cho, J.-H., Yim, K.-O., Lee, H.-I., Baeg, J.-H. and Cha, J.-S. 2011. Detection of the causal agent of bacterial wilt, Ralstonia solanacearum in the seeds of solanaceae by PCR. Res. Plant Dis. 17:184-190.   DOI
7 Denny, T. 2006. Plant pathogenic Ralstonia species. In: Plant-Associated Bacteria, ed. by S. S. Gnanamanickam, pp. 573-644. Springer, Dordrecht, Netherlands.
8 Elphinstone, J. G. 2005. The current bacterial wilt situation: a global overview. In: Bacterial wilt: the disease and the Ralstonia solanacearum species complex, eds. by C. Allen, P. Prior and A. C. Hayward, pp. 9-28. American Phytopathological Society, St. Paul, MN, USA.
9 Fegan, M. and Prior, P. 2005. How complex is the Ralstonia solanacearum species complex, pp. 449-462. American Phytopathological Society, St. Paul, MN, USA.
10 Genin, S. and Denny, T. P. 2012. Pathogenomics of the Ralstonia solanacearum species complex, pp. 67-89. In: Annual Review of Phytopathology, Vol 50, eds. by N. K. VanAlfen, J. E. Leach and S. Lindow.
11 Han, Y.-K., Min, J.-S., Park, J.-H., Han, K.-S., Kim, D.-H., Lee, J.-S. and Kim, H.-H. 2009. Screening of tomato cultivars resistant to bacterial wilts. Res. Plant Dis. 15:198-201.   DOI
12 Sakthivel, K., Gautam, R. K., Kumar, K., Roy, S. D., Kumar, A., Devendrakumar, C., Vibhuti, M., Neelam, S. and Vinatzer, B. A. 2016. Diversity of Ralstonia solanacearum strains on the Andaman islands in India. Plant Dis. 100:732-738.   DOI
13 Seo, S.-T., Park, J.-H., Han, K.-S., Cheong, S.-R. and Lee, S. 2007. Genetic diversity of Ralstonia solanacearum strains isolated from pepper and tomato plants in Korea. Res. Plant Dis. 13:24-29.   DOI
14 Wicker, E., Grassart, L., Coranson-Beaudu, R., Mian, D., Guilbaud, C., Fegan, M. and Prior, P. 2007. Ralstonia solanacearum strains from Martinique (French west indies) exhibiting a new pathogenic potential. Appl. Environ. Microbiol. 73:6790-6801.   DOI
15 Hayward, A. C. 1964. Characteristics of Pseudomonas solanacearum. J. Appl. Bacteriol. 27:265-277.   DOI
16 Yun, G.-S., Park, S.-Y., Kang, H. J., Lee, K. Y. and Cha, J.-S. 2004. Contamination level of Ralstonia solanacearum in soil of greenhouses cultivating tomato plants in Chungbuk province and characteristics of the isolates. Res. Plant Dis. 10:58-62.   DOI
17 Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29:65-87.   DOI
18 He, L., Sequeira, L. and Kelman, A. 1983. Characteristics of strains of Pseudomonas solanacearum from China. Plant Dis. 67:1357-1361.   DOI
19 Jeong, Y., Kim, J., Kang, Y., Lee, S. and Hwang, I. 2007. Genetic diversity and distribution of Korean isolates of Ralstonia solanacearum. Plant Dis. 91:1277-1287.   DOI
20 Jeong, Y., Cheong, H., Choi, O., Kim, J. K., Kang, Y., Kim, J., Lee, S., Koh, S., Moon, J. S. and Hwang, I. 2011. An HrpB-dependent but type III-independent extracellular aspartic protease is a virulence factor of Ralstonia solanacearum. Mol. Plant Pathol. 12:373-380.   DOI
21 Jung, E. J., Joo, H. J., Choi, Y., Lee, S. Y., Jung, Y. H., Lee, M. H., Kong, H. G. and Lee, S.-W. 2014. Resistance evaluation of tomato germplasm against bacterial wilt by Ralstonia solanacearum. Res. Plant Dis. 20:253-258.   DOI
22 Kang, M. J., Lee, M. H., Shim, J. K., Seo, S. T., Shrestha, R., Cho, M. S., Hahn, J. H. and Park, D. S. 2007. PCR-based specific detection of Ralstonia solanacearum by amplification of cytochrome c1 signal peptide sequences. J. Microbiol. Biotechnol. 17:1765-1771.
23 Kelman, A. 1954. The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance in a tetrazolium medium. Phytopathology 44:693-695.
24 Kim, S. G., Hur, O.-S., Ro, N.-Y., Ko, H.-C., Rhee, J.-H., Sung, J. S., Ryu, K.-Y., Lee, S.-Y. and Baek, H. J. 2016. Evaluation of resistance to Ralstonia solanacearum in tomato genetic resources at seedling stage. Plant Pathol. J. 32:58-64.   DOI
25 Lebeau, A., Daunay, M. C., Frary, A., Palloix, A., Wang, J. F., Dintinger, J., Chiroleu, F., Wicker, E. and Prior, P. 2011. Bacterial wilt resistance in tomato, pepper, and eggplant: genetic resources respond to diverse strains in the Ralstonia solanacearum species complex. Phytopathology 101:154-165.   DOI
26 Lee, H. J., Jo, E. J., Kim, N. H., Chae, Y. and Lee, S.-W. 2011. Disease responses of tomato pure lines against Ralstonia solanacearum strains from Korea and susceptibility at high temperature. Res. Plant Dis. 17:326-333.   DOI
27 Lee, S. D. 1999. Occurrence and characterization of major plant bacterial diseases in Korea. Ph.D. thesis. Seoul National University, Seoul, Korea.
28 Lee, Y. K. and Kang, H. W. 2013. Physiological, biochemical and genetic characteristics of Ralstonia solanacearum strains isolated from pepper plants in Korea. Res. Plant Dis. 19:265-272.   DOI
29 Lim, Y.-S., Lee, M.-J., Cheung, J.-D., Rew, Y.-H. and Kim, B.-S. 2008. Occurrence and biovar classification of bacterial wilt caused by Ralstonia solanacearum in eggplant (Solanum melongena). Res. Plant Dis. 14:10-14.   DOI
30 Martin, C. and French, E. R. 1985. Bacterial wilt of potato (Pseudomonas solanacearum). International Potato Center.
31 Park, S., Gupta, R., Krishna, R., Kim, S. T., Lee, D. Y., Hwang, D. J., Bae, S. C. and Ahn, I. P. 2016. Proteome analysis of disease resistance against Ralstonia solanacearum in potato cultivar CT206-10. Plant Pathol. J. 32:25-32.   DOI
32 Pegg, K. and Moffett, M. L. 1971. Host range of the ginger strain of Pseudomonas solanacearum in Queensland. Animal Prod. Sci. 11:696-698.   DOI
33 Poussier, S., Prior, P., Luisetti, J., Hayward, C. and Fegan, M. 2000. Partial sequencing of the hrpB and endoglucanase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex. Syst. Appl. Microbiol. 23:479-486.   DOI
34 Prior, P. and Fegan, M. 2005. Recent developments in the phylogeny and classification of Ralstonia solanacearum. pp. 127-136. In: I International Symposium on Tomato Diseases 695.