• BMB Reports
• /
• 제35권6호
• /
• pp.562-567
• /
• 2002

In order to find the cellular interaction factors of the Heliothis armigera nuclear polyhedrosis virus capsid protein VP39, a Heliothis armigera cell cDNA library was constructed. Then VP39 was used as bait. The host actin gene was isolated from the cDNA library with the yeast two-hybrid system. This demonstrated that VP39 could interact with its host actin in yeast. In order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pEGFP39. The fusion protein was expressed in the Hz-AM1 cells under the control of the Autographa californica multiple nucleopolyhedrovirus immediate early gene promoter. The host actin was labeled specifically by the red fluorescence substance, tetramethy rhodamine isothicyanete-phalloidin. Observation under a fluorescence microscopy showed that VP39, which was indicated by green fluorescence, began to appear in the cells 6 h after being transfected with pEGFP39. Red actin cables were also formed in the cytoplasm at the same time. Actin was aggregated in the nucleus 9 h after the transfection. The green and red fluorescence always appeared in the same location of the cells, which demonstrated that VP39 could combine with the host actin. Such a combination would result in the actin skeleton rearrangement.

• 천문학회보
• /
• 제44권1호
• /
• pp.77.3-77.3
• /
• 2019

Aims. The evolution of scaling relations between SMBHs and their host galaxies becomes uncertain at high redshifts. The HULQ project proposes to use gravitational lensing to measure the masses of QSO host galaxies, an otherwise difficult goal. SMBH masses of QSOs are relatively easy to determine using either reverberation mapping or the single-epoch method. These measurements, if made for a substantial number of QSOs at various redshifts, will allow us to study the co-evolution of SMBHs and their host galaxies. To determine the feasibility of this study, we present how to estimate the number of sources lensed by QSO hosts, i.e. the number of deflector QSO host galaxies (hereafter QSO lenses). Method and results. Using SMBH masses measured from SDSS DR14 spectra, and the M_BH - Sigma relation, the Einstein radii are calculated as a function of source redshift, assuming singular isothermal sphere mass distributions. Using QSOs and galaxies as sources, the probability of a QSO host galaxy being a QSO lens is calculated, depending on the limiting magnitude. The expected numbers of QSO lenses are estimated for ongoing and future wide-imaging surveys, and additional factors that may affect these numbers are discussed.

• 한국미생물·생명공학회지
• /
• 제50권4호
• /
• pp.441-456
• /
• 2022

Shiga toxins (Stxs) are major virulence factors from the enterohemorrhagic Escherichia coli (EHEC), a subset of Stx-producing Escherichia coli. Stxs are multi-functional, ribosome-inactivating proteins that underpin the development of hemolytic uremic syndrome (HUS) and central nervous system (CNS) damage. Currently, therapeutic options for the treatment of diseases caused by Stxs are limited and unsatisfactory. Furthermore, the pathophysiological mechanisms underpinning toxin-induced inflammation remain unclear. Numerous works have demonstrated that the various host ribotoxic stress-induced targets including p38 mitogen-activated protein kinase, its downstream substrate Mitogen-activated protein kinase-activated protein kinase 2, and apoptotic signaling via ER-stress sensors are activated in many different susceptible cell types following the regular retrograde transportation of the Stxs, eventually leading to disturbing intercellular communication. Therapeutic options targeting host cellular pathways induced by Stxs may represent a promising strategy for intervention in Stx-mediated acute renal dysfunction, retinal damage, and CNS damage. This review aims at fostering an in-depth understanding of EHEC Stxs-mediated pathogenesis through the toxin-host interactions.

• 한국전자거래학회지
• /
• 제24권2호
• /
• pp.55-70
• /
• 2019

통신 인프라 구축과 최적화를 위해서는 각 구성 요소들의 연관성을 고려할 필요가 있다. 본 논문에서는 최적화된 통신 인프라 구축을 위해 필요한 주요 고려사항을 바탕으로 성능이 향상된 MACSec 기반의 통신 인프라 구성 방안을 제시한다. 제안된 MACSec 기반 기술은 통신 인프라를 처음부터 다시 설계하지 않고 IPSec 기술을 별도의 장비를 추가하지 않고 대체할 수 있다. 아울러, 구성 시 주요 고려사항인 메시지 오버헤드와 암호화 처리 성능, 그리고 이동성 측면에서 실험을 통해 IPSec과 성능을 평가한다. 시험 결과에 따르면 MACSec으로 구현된 암호화 네트워크에서 IPSec보다 Hop 지연과 메시지 오버헤드와 같은 일반적 성능뿐만 아니라 연결 지점 변경 시험을 통해 이동성 성능이 우위에 있다.

• IMMUNE NETWORK
• /
• 제21권3호
• /
• pp.19.1-19.18
• /
• 2021

Clinical and molecular phenotypes of asthma are complex. The main phenotypes of adult asthma are characterized by eosinophil and/or neutrophil cell dominant airway inflammation that represent distinct clinical features. Upper and lower airways constitute a unique system and their interaction shows functional complementarity. Although human upper airway contains various indigenous commensals and opportunistic pathogenic microbiome, imbalance of this interactions lead to pathogen overgrowth and increased inflammation and airway remodeling. Competition for epithelial cell attachment, different susceptibilities to host defense molecules and antimicrobial peptides, and the production of proinflammatory cytokine and pattern recognition receptors possibly determine the pattern of this inflammation. Exposure to environmental factors, including infection, air pollution, smoking is commonly associated with asthma comorbidity, severity, exacerbation and resistance to anti-microbial and steroid treatment, and these effects may also be modulated by host and microbial genetics. Administration of probiotic, antibiotic and corticosteroid treatment for asthma may modify the composition of resident microbiota and clinical features. This review summarizes the effect of some environmental factors on the upper respiratory microbiome, the interaction between host-microbiome, and potential impact of asthma treatment on the composition of the upper airway microbiome.

• 미생물과산업
• /
• 제19권4호
• /
• pp.14-26
• /
• 1993

Optimizing protein production in recombinant E. coli strains involves manipulation of genetic and environmental factors. In designing a production system, attention must be paid to gene expression efficiency, culture conditions and bioreactor configuration. Although not much emphasis was given to the physiology of host strains in this review, an understanding of the relationship between the physiology of host cell growth and the overproduction of a cloned gene protein is of primary importance to the improvement of the recombinant fermentation processes. Sometimes it is desirable to make use of gene fusion systems, e.g. protein A, polypeptide, gutathione-S-transferase, or pneumococcal murein hydrolase fusion, to facilitate protein purification.

• 한국응용곤충학회지
• /
• 제51권4호
• /
• pp.331-342
• /
• 2012

본 연구는 먹이식물과 흡밀식물의 수 그리고 식생이 나비의 개체수 및 다양성에 미치는 영향을 조사하기 위하여 2008-09년까지 충북 보은군 속리산 법주사 지역을 4개 구간으로 나누어 모니터링을 실시하였으며 이를 단순회귀분석 및 다중회귀분석하였다. 그 결과 나비 개체수와 식물종수, 흡밀식물 그리고 먹이식물 수를 다중회귀분석 한 결과에서 상관관계를 보였으며, 서식지 면적이 클수록 개체수는 많아지는 것으로 나타났다. 그러나 단순회귀 분석 시 나비의 종수와 다양도지수는 먹이식물 수 및 구간면적과 각각 낮은 상관관계를 나타냈다. 또한 보다 다양한 식생을 보이는 구간에서 나비 종수가 증가하였다. 나비의 다양성과 개체군 보전을 위해서는 다양한 수종의 식물과 함께 나비의 먹이식물과 흡밀식물이 복합적으로 분포하고, 식생은 각각의 요소들이 서로 보완관계를 유지할 수 있도록 다른 형태로 존재하는 것이 필요함을 시사한다.

• 한국융합학회논문지
• /
• 제8권1호
• /
• pp.131-138
• /
• 2017

연구의 목적은 대나무의 고장 전라남도 담양군에서 열린 2015 담양 세계 대나무 박람회 개최에 따른 지역발전 영향요인이 지역애착도와 개최지지 및 개최 속성에 미치는 영향을 파악하고자 하였다. 연구를 수행하기 위하여 가설을 설정 한 뒤 박람회장에 방문객을 대상으로 설문조사를 후 표본을 추출하여 설문조사를 통해 실증분석을 하였다. 박람회 개최로 인해 지역이 발전하고 향후에 관광객들이 다시 찾는 관광 융합 연구의 목적을 제시하고자 한다 . 지역 애착도는 환경요인과 사회요인에 영향을 미친 것으로 나타났고. 개최지지, 개최지속성은 환경요인가 사회요인이 가장 큰 영향을 미치는 것으로 나타났고, 경제요인, 환경요인 순으로 영향을 미치는 것으로 나타났으며, 개최지지가 지역애착도 요인에 영향을 미치는 것으로 나타났다. 결과적으로 박람회 개최에 따른 지역발전 영향요인이 지역애착도와 개최지지 및 개최속성에 미치는 영향을 검증을 통해 향후 국내 지역박람회와 축제 평가에 관한 전략 모색에 기여할 수 있을 것이다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권18호
• /
• pp.8041-8045
• /
• 2016

Cervical cancer is one of the most common cancers in women worldwide. During their life time the vast majority of women become infected with human papillomavirus (HPV), but interestingly only a small portion develop cervical cancer and in the remainder infection regresses to a normal healthy state. Beyond HPV status, associated molecular characterization of disease has to be established. However, initial work suggests the existence of several different molecular classes, based on the biological features of differentially expressed genes in each subtype. This suggests that additional risk factors play an important role in the outcome of infection. Host genomic factors play an important role in the outcome of such complex or multifactor diseases such as cervical cancer and are also known to regulate the rate of disease progression. The aim of this review was to compile advances in the field of host genomics of HPV positive and negative cervical cancer and their association with clinical response.

• Journal of Microbiology
• /
• 제43권5호
• /
• pp.443-450
• /
• 2005

The multi-host pathogen, Pseudomonas aeruginosa, possesses an extraordinary versatility which makes it capable of surviving the adverse conditions provided by environmental, host, and, presumably, competing microbial factors in its natural habitats. Here, we investigated the P. aeruginosa-Bacillus subtilis interaction in laboratory conditions and found that some P. aeruginosa strains can outcompete B. subtilis in mixed planktonic cultures. This is accompanied by the loss of B. subtilis viability. The bactericidal activity of P. aeruginosa is measured on B. subtilis plate cultures. The bactericidal activity is attenuated in pqsA, mvfR, lasR, pilB, gacA, dsbA, rpoS, and phnAB mutants. These results suggest that P. aeruginosa utilizes a subset of conserved virulence pathways in order to survive the conditions provided by its bacterial neighbors.