• BMB Reports
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• v.35 no.6
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• pp.562-567
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• 2002

In order to find the cellular interaction factors of the Heliothis armigera nuclear polyhedrosis virus capsid protein VP39, a Heliothis armigera cell cDNA library was constructed. Then VP39 was used as bait. The host actin gene was isolated from the cDNA library with the yeast two-hybrid system. This demonstrated that VP39 could interact with its host actin in yeast. In order to corroborate this interaction in vivo, the vp39 gene was fused with the green fluorescent protein gene in plasmid pEGFP39. The fusion protein was expressed in the Hz-AM1 cells under the control of the Autographa californica multiple nucleopolyhedrovirus immediate early gene promoter. The host actin was labeled specifically by the red fluorescence substance, tetramethy rhodamine isothicyanete-phalloidin. Observation under a fluorescence microscopy showed that VP39, which was indicated by green fluorescence, began to appear in the cells 6 h after being transfected with pEGFP39. Red actin cables were also formed in the cytoplasm at the same time. Actin was aggregated in the nucleus 9 h after the transfection. The green and red fluorescence always appeared in the same location of the cells, which demonstrated that VP39 could combine with the host actin. Such a combination would result in the actin skeleton rearrangement.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.77.3-77.3
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• 2019

Aims. The evolution of scaling relations between SMBHs and their host galaxies becomes uncertain at high redshifts. The HULQ project proposes to use gravitational lensing to measure the masses of QSO host galaxies, an otherwise difficult goal. SMBH masses of QSOs are relatively easy to determine using either reverberation mapping or the single-epoch method. These measurements, if made for a substantial number of QSOs at various redshifts, will allow us to study the co-evolution of SMBHs and their host galaxies. To determine the feasibility of this study, we present how to estimate the number of sources lensed by QSO hosts, i.e. the number of deflector QSO host galaxies (hereafter QSO lenses). Method and results. Using SMBH masses measured from SDSS DR14 spectra, and the M_BH - Sigma relation, the Einstein radii are calculated as a function of source redshift, assuming singular isothermal sphere mass distributions. Using QSOs and galaxies as sources, the probability of a QSO host galaxy being a QSO lens is calculated, depending on the limiting magnitude. The expected numbers of QSO lenses are estimated for ongoing and future wide-imaging surveys, and additional factors that may affect these numbers are discussed.

• Microbiology and Biotechnology Letters
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• v.50 no.4
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• pp.441-456
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• 2022

Shiga toxins (Stxs) are major virulence factors from the enterohemorrhagic Escherichia coli (EHEC), a subset of Stx-producing Escherichia coli. Stxs are multi-functional, ribosome-inactivating proteins that underpin the development of hemolytic uremic syndrome (HUS) and central nervous system (CNS) damage. Currently, therapeutic options for the treatment of diseases caused by Stxs are limited and unsatisfactory. Furthermore, the pathophysiological mechanisms underpinning toxin-induced inflammation remain unclear. Numerous works have demonstrated that the various host ribotoxic stress-induced targets including p38 mitogen-activated protein kinase, its downstream substrate Mitogen-activated protein kinase-activated protein kinase 2, and apoptotic signaling via ER-stress sensors are activated in many different susceptible cell types following the regular retrograde transportation of the Stxs, eventually leading to disturbing intercellular communication. Therapeutic options targeting host cellular pathways induced by Stxs may represent a promising strategy for intervention in Stx-mediated acute renal dysfunction, retinal damage, and CNS damage. This review aims at fostering an in-depth understanding of EHEC Stxs-mediated pathogenesis through the toxin-host interactions.

• The Journal of Society for e-Business Studies
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• v.24 no.2
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• pp.55-70
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• 2019

It is essential to consider the relationships between each component in the communication infrastructure in order to build and optimize the infrastructure. In this paper, based on the major factors to consider for the optimized communication infrastructure, we propose an enhanced MACSec-based deployment mechanism for communication infrastructure. The proposed MACSec mechanism can replace the IPSec without the additional devices and redesign of the communication infrastructure. In addition, we evaluate the performance of MACSec and IPSec in terms of the major factors such as message overhead, encryption processing, and host mobility. According to the evaluation results, we can say that MACSec is superior to IPSec with regard to mobility as well as hop delay and message overhead.

• IMMUNE NETWORK
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• v.21 no.3
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• pp.19.1-19.18
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• 2021

Clinical and molecular phenotypes of asthma are complex. The main phenotypes of adult asthma are characterized by eosinophil and/or neutrophil cell dominant airway inflammation that represent distinct clinical features. Upper and lower airways constitute a unique system and their interaction shows functional complementarity. Although human upper airway contains various indigenous commensals and opportunistic pathogenic microbiome, imbalance of this interactions lead to pathogen overgrowth and increased inflammation and airway remodeling. Competition for epithelial cell attachment, different susceptibilities to host defense molecules and antimicrobial peptides, and the production of proinflammatory cytokine and pattern recognition receptors possibly determine the pattern of this inflammation. Exposure to environmental factors, including infection, air pollution, smoking is commonly associated with asthma comorbidity, severity, exacerbation and resistance to anti-microbial and steroid treatment, and these effects may also be modulated by host and microbial genetics. Administration of probiotic, antibiotic and corticosteroid treatment for asthma may modify the composition of resident microbiota and clinical features. This review summarizes the effect of some environmental factors on the upper respiratory microbiome, the interaction between host-microbiome, and potential impact of asthma treatment on the composition of the upper airway microbiome.

• The Microorganisms and Industry
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• v.19 no.4
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• pp.14-26
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• 1993

Optimizing protein production in recombinant E. coli strains involves manipulation of genetic and environmental factors. In designing a production system, attention must be paid to gene expression efficiency, culture conditions and bioreactor configuration. Although not much emphasis was given to the physiology of host strains in this review, an understanding of the relationship between the physiology of host cell growth and the overproduction of a cloned gene protein is of primary importance to the improvement of the recombinant fermentation processes. Sometimes it is desirable to make use of gene fusion systems, e.g. protein A, polypeptide, gutathione-S-transferase, or pneumococcal murein hydrolase fusion, to facilitate protein purification.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.331-342
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• 2012

The effects of number of host plants and nectar plants on the number and diversity of butterflies were monitored and analyzed by simple and multiple regression from May 2008 to Sep 2009 in the Bubjusa region (Mt. Sokri, Boeun-gun, Chungcheongbuk-do), which was divided into four regions. The results demonstrate that the number of butterflies has a positive correlation with the number of host plant species, the number of nectar plant species, and the number of host plants multiple regression analysis. The number of butterflies also increased habitat area. However, the butterfly species number and diversity index showed lower correlation with the number of host plants and the area size of the divided region, respectively, when analyzed by simple regression. In addition, increased species diversity in a region with more diverse plant flora. These results suggest that the composite distribution of host and nectar plants with diverse plant flora is required for butterfly diversity and population preservation. Additionally, it also suggest that vegetation types complementarity all factors.

• Journal of the Korea Convergence Society
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• v.8 no.1
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• pp.131-138
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• 2017

The purpose of research and bamboo for failure, Damyang-county, Jeollanam-do at 2015 Damyang bamboo the world fairs held according to the attachment to local factors impact of regional development and hosting support. To identify the impact on the property held. Fair after setting up a hypothesis to carry out research in the visitors through after sample surveys and survey and Empirical Analysis. Show areas are improving in the future, due to host tourists want to come up with the purpose of research to become a popular tourist attraction again. Attachment or environmental factors and social factors showed that, and affect the region. The host of social factors turned out to be the biggest influence, and economic factors, environmental factors shown to affect, and to a venue in order.Attachment is a factor appears to be affecting the region was the venue. The property is shown to environmental factors is not statistically significant, and host local affection, support appears to be factors affecting the holding. As a result, Fair, held regional attachment to the Influence Factors of regional development support, and according to the next fair in the Region and through the impact on the property held.Evaluation strategy could contribute to explore the festival.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8041-8045
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• 2016

Cervical cancer is one of the most common cancers in women worldwide. During their life time the vast majority of women become infected with human papillomavirus (HPV), but interestingly only a small portion develop cervical cancer and in the remainder infection regresses to a normal healthy state. Beyond HPV status, associated molecular characterization of disease has to be established. However, initial work suggests the existence of several different molecular classes, based on the biological features of differentially expressed genes in each subtype. This suggests that additional risk factors play an important role in the outcome of infection. Host genomic factors play an important role in the outcome of such complex or multifactor diseases such as cervical cancer and are also known to regulate the rate of disease progression. The aim of this review was to compile advances in the field of host genomics of HPV positive and negative cervical cancer and their association with clinical response.

• Journal of Microbiology
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• v.43 no.5
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• pp.443-450
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• 2005

The multi-host pathogen, Pseudomonas aeruginosa, possesses an extraordinary versatility which makes it capable of surviving the adverse conditions provided by environmental, host, and, presumably, competing microbial factors in its natural habitats. Here, we investigated the P. aeruginosa-Bacillus subtilis interaction in laboratory conditions and found that some P. aeruginosa strains can outcompete B. subtilis in mixed planktonic cultures. This is accompanied by the loss of B. subtilis viability. The bactericidal activity of P. aeruginosa is measured on B. subtilis plate cultures. The bactericidal activity is attenuated in pqsA, mvfR, lasR, pilB, gacA, dsbA, rpoS, and phnAB mutants. These results suggest that P. aeruginosa utilizes a subset of conserved virulence pathways in order to survive the conditions provided by its bacterial neighbors.