• Applied Microscopy
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• 제48권4호
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• pp.96-101
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• 2018

The release of nanoscale membrane-bound vesicles is common in all three domains of life. These vesicles are involved in a variety of biological processes such as cell-to-cell communication, horizontal gene transfer, and substrate transport. Prokaryotes including bacteria and archaea release membrane vesicles (MVs) (20 to 400 nm in diameter) into their extracellular milieu. In spite of structural differences in cell envelope, both Gram-positive and negative bacteria produce MVs that contain the cell membrane of each bacterial species. Archaeal MVs characteristically show surface-layer encircling the vesicles. Filamentous fungi and yeasts as eukaryotic microbes produce bilayered exosomes that have varying electron density. Microbes also form intracellular vesicles and minicells that are similar to MVs and exosomes in shape. Electron and fluorescence microscopy could reveal the presence of DNA in MVs and exosomes. Given the biogenesis of extracellular vesicles from the donor cell, in situ high-resolution microscopy can provide insights on the structural mechanisms underlying the formation and release of microbial extracellular vesicles.

• Journal of Periodontal and Implant Science
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• 제39권3호
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• pp.367-373
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• 2009

Purpose: Following tooth extraction caused by severe periodontitis, alveolar ridge dimension lose their original volume. To reduce the alveolar ridge dimension, the ridge preservation technique has been introduced and tested in many clinical studies with membrane alone or membrane plus graft, achieving reduced ridge loss compared to extraction only. The aim of the present clinical study was to compare the post-extraction dimensional changes in the membrane exposure group to non-exposure group during healing period following ridge preservation technique. Methods: Ridge preservation was performed in 44 extraction sites. After extraction, deproteinized bovine bone mineral coated with synthetic oligopeptide (Ossgen-$X15^{(R)}$) or deproteinized bovine bone mineral (Bio-$Oss^{(R)}$) was implanted into the socket. A collagen membrane (Bio-$Gide^{(R)}$) was trimmed to cover the socket completely and applied to the entrance of the socket. Four clinical parameters were compared between baseline and 6 months. Results: During healing period, membrane exposure was observed at 19 sites. At the re-entry, hard newly formed tissue were observed at the ridge preservation site. The grafted socket sites were well preserved in their volume dimension. In both groups, horizontal ridge width was reduced and vertical height was increased. There were not statistically significant differences in horizontal (-1.32 mm vs -1.00 mm) and vertical ridge change (2.24 mm vs 2.37 mm at buccal crest, 1.36 mm vs. 1.53 mm at lingual crest) between two groups. Conclusions: The ridge preservation approach after tooth extraction effectively prevented resorption of hard tissue ridge in spite of membrane exposure during healing period.

• Journal of Periodontal and Implant Science
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• 제50권6호
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• pp.406-417
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• 2020

Purpose: This study investigated whether the placement of ribose cross-linked collagen (RCLC) membranes without primary soft tissue closure predictably resulted in sufficient alveolar ridge preservation in contained and non-contained extraction sockets. Methods: Membranes were positioned across extraction sockets, undermining full-thickness flaps, and the gingival margins were fixed by double-interrupted sutures without crossed horizontal mattress sutures for 1 week. In non-contained sockets, a bone substitute was used to support the membrane within the bony envelope. Radiographs and clinical images obtained 4 months later were analyzed by ImageJ software using non-parametric tests. Results: In 18 patients, 20 extraction sockets healed uneventfully and all sites received standard-diameter implants (4.1, 4.8, or 5.0 mm) without additional bone augmentation. Soft tissues and the muco-gingival border were well maintained. A retrospective analysis of X-rays and clinical photographs showed non-significant shrinkage in the vertical and horizontal dimensions (P=0.575 and P=0.444, respectively). The new bone contained vital bone cells embedded in mineralized tissues. Conclusions: Within the limitations of this pilot study, open healing of RCLC membranes may result in sufficient bone volume for implant placement without additional bone augmentation in contained and non-contained extraction sockets.

• Journal of Periodontal and Implant Science
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• 제45권6호
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• pp.229-237
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• 2015

Purpose: The aim of this study was to determine the clinical feasibility of using dehydrothermally cross-linked collagen membrane (DCM) for bone regeneration around peri-implant dehiscence defects, and compare it with non-cross-linked native collagen membrane (NCM). Methods: Dehiscence defects were investigated in twenty-eight patients. Defect width and height were measured by periodontal probe immediately following implant placement (baseline) and 16 weeks afterward. Membrane manipulation and maintenance were clinically assessed by means of the visual analogue scale score at baseline. Changes in horizontal thickness at 1 mm, 2 mm, and 3 mm below the top of the implant platform and the average bone density were assessed by cone-beam computed tomography at 16 weeks. Degradation of membrane was histologically observed in the soft tissue around the implant prior to re-entry surgery. Results: Five defect sites (two sites in the NCM group and three sites in the DCM group) showed soft-tissue dehiscence defects and membrane exposure during the early healing period, but there were no symptoms or signs of severe complications during the experimental postoperative period. Significant clinical and radiological improvements were found in all parameters with both types of collagen membrane. Partially resorbed membrane leaflets were only observed histologically in the DCM group. Conclusions: These findings suggest that, compared with NCM, DCM has a similar clinical expediency and possesses more stable maintenance properties. Therefore, it could be used effectively in guided bone regeneration around dehiscence-type defects.

• Journal of Periodontal and Implant Science
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• 제36권2호
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• pp.427-434
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• 2006

The form of furcation influence both the pathogenesis of periodontal destruction and therapeutic results. The present study was performed to evaluate the effect of root trunk length on clinical outcomes of guided tissue regeneration. Total 30 mandibular first molars were evaluated in this study. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were measured at baseline and 6 month after GTR. Correlation coefficients between root trunk length and other clinical measurement were analyzed. The results of this study were as follows 1. The mean root trunk length in lower 1st molar was 2.15 mm. 2. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were significantly reduced at 6 month postoperatively compared to values of baseline 3. Correlation coefficient between root trunk length and vertical defect depth at baseline was 0.406 showing the positive correlation 4. Correlation coefficient between root trunk length and horizontal defect depth at baseline was -0.463 showing the negative correlation. 5. Correlation coefficient between root trunk length and decrease of horizontal defect depth after GTR was 0.654 showing the positive correlation. In conclusion, the root trunk length maybe effector for clinical outcome after guided tissue regeneration.

• Journal of Periodontal and Implant Science
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• 제35권4호
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• pp.877-889
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• 2005

This study was designed to compare the effects of treatment using chitosan membrane $(Nanogide-C^{(R)})$ resorbable barrier with control treated by polylactic acid/polylacticglycolic acid membrane(PLA/PLGA membrane, $Biomesh^{(R)}$). 44 furcation defecs from 44 patients with class 2 furcation degree were used for this study, 22 sites of them were treated by chitosan membrane as experimental group and 22 site were treated by PLA/PLGA membrane as control group. Clinical parameters including probing depth, gingival recession, attachment level and radiographic examination were evlauated at base line, 1 month, 2 month and 3 month. after surgery. Statistical test used to analyze these data included paired t-test, one way ANOVA. The results are as follows : 1. Probing depth was significanlly decreased in the two group and there were significant differences between groups(p<0.05). 2. Gingival recession was not significanlly increased in the two group and there were no significant differences between groups(p<0.05). 3. Loss of attachment was statistically decreased in the two group and there were no significant differences between groups(p<0.05). 4. Horizontal bone level was significanlly increased in the two group and there were significant differences between groups(p<0.05). On the basis of these results, chitoans resorbable membrane has similar potential to PLA/PLGA membrane in GTR for furcation defect.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.80-88
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• 1996

The aim of the present investigation was to see the effect of combined use of PDGF BB and IGF -1 on the guided tissue regeneration(GTR) using barrier membrane in the treatment of human furcation involvement. Twelve patients with initially diagnosed as having moderate to advanced adult periodontitis with mandibular class II buccal furcation defects have been wer selected. Initial scaling and root planing has been performed and baseline data consisting of probing depths and attachment levels have been recorded prior to surgical procedures. The GTR procedures using either barrier membrane(control : ePTFE) alone or together with the application of PDGF - BB and IGF -l(experimental : ePTFE+PDGF/IGF) have been done under the routine guidelines. During the surgery, the distance from CEJ either to the bottom of the bone defects(CEJ - BD) or to the bone crest(CEJ-BC) were measured. Horizontal distance to the deepest area in the furcal defects were measured from the reference line connection the most prominent bony walls of the two buccal roots. 6 months following the GTR therapy, all the measurements were made repeatedly. The probing attachment gain of the experimental and the control grous were 2.14mm and l.07mm, respectively with no statically significnant difference. Amont of vertical bone fill in the experimental and the control groups were 2.43mm and 2.29mm, rexpectively. Amonut of horizontal bone fill were 2.86mm in the experimental group and 2.17mm in the control group, respectively. However, there were no significant differences in the amount of bone fill(both vertical and horizontal)between the two groups.

• 대한토목학회논문집
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• 제29권6D호
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• pp.719-726
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• 2009

본 연구에서는 슬래브와 보조기층 간의 마찰 특성을 조사하기 위하여 국내 콘크리트 포장에 주로 사용되는 린 콘크리트, 쇄석, 아스팔트 보조기층에 대하여 마찰력 실험을 실시하였다. 이 외에도 분리막 사용 여부와 보조기층의 습윤 상태를 실험변수로 사용하였다. 슬래브에 40mm/hour의 속도로 수평하중을 가한 1차 하중 재하 시와 안정화 상태(2차 및 3차) 및 습윤상태(4차)에서의 슬래브의 수평변위와 마찰계수를 측정하였다. 1차 하중재하 시 린 콘크리트, 아스팔트, 쇄석, 그리고 분리막을 사용한 보조기층 순으로 최대 마찰계수가 컸으며, 안정화 상태에서는 아스팔트, 쇄석, 린 콘크리트 그리고 분리막을 사용한 보조기층 순으로 크게 측정되었다. 분리막을 사용한 보조기층은 습윤 상태에 의한 영향이 거의 없었으나 분리막을 사용하지 않은 쇄석 및 아스팔트 보조기층은 습윤 상태로 인하여 마찰계수가 감소하였다. 향후 슬래브의 두께와 아스팔트의 온도 민감성이 미치는 영향에 대한 추가 마찰력 실험이 수행될 것이다. 그리고, 실험 결과를 이용한 구조해석을 통하여 슬래브와 보조기층 간의 마찰특성이 콘크리트 포장의 거동 및 공용성에 미치는 영향을 조사할 계획이다.

• 한국건설관리학회논문집
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• 제6권3호
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• pp.128-134
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• 2005

방수공사의 중요성에 의해 그간 다양한 방수 재료 및 공법이 개발되었고 옥상바닥 방수공법으로 현재 합성고분자의 신장성, 탄성, 내구성 등이 뛰어날 폴리우레탄 도막방수재가 많이 보편화되어 성능이 높아지면서 시장점유율도 급증하게 되었다. 이에 방수성은 향상되었지만 이것 또한 방수결함이 빈번히 발생되고 있어 하자발생률이 감소되지는 않고 있는 실정이다. 이러한 측면에서 본 연구는 옥상방수에 있어서 폴리우레탄 도박방수를 중심으로 방수결함 유형 및 하자원인을 분석함으로써 향후 하자 재발방지를 위한 기초 자료로 활용하고자 한다. 순천지역을 대상으로 한 13개 현장 조사 결과 바탕의 평면불량으로 인한 물고임 현상이 $25\%$로 가장 많이 나타났고, 바탕의 건조불량으로 인한 부풀음 박리 현상이 $15\%$ 순으로 나타났으며 들뜸 $14\%$, 파단 $11\%$, 기타 순으로 결함이 발생되었다.

• 한국공간구조학회논문집
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• 제2권3호
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• pp.81-92
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• 2002

The objective of this paper is to help to make decision of the appropriate structural types in long span structured building due to range of span. For the intention, based on 7 forces of structural element, it is analized the relationships among 6 configurations of structural element(d/1), 25 structural types, 4 materials, and span-length known with 186 sample from 1850 to 1996. 1) bending forces: $club(1/100{\sim}1/10),\;plate(1/100{\sim}1/10),\;rahmen(steel,\;10{\sim}24m)\;simple\;beam(PC,\;10{\sim}35m)$ 2) shearing forces: $shell(1/100{\sim}1/1000)\;hyperbolic\;paraboloids(RC,25{\sim}97m)$ 3) shearing+bending forces: plate, folded $plate(RC21{\sim}59m)$ 4) compression axial forces: club, $arch(RC,\;32{\sim}65m)$ 5) compression+tension forces: shell, braced dome $shell(RC,\;40{\sim}201m),\;vault\;shell(RC,\;16{\sim}103m)$ 6) compression+tension axial forces: $rod(1/1000{\sim}1/100)$, cable(below 1/1000)+rod, coble+rod+membrane(below 1/1000), planar $truss(steel,\;31{\sim}134m),\;arch\;truss(31{\sim}135m),\;horizontal\;spaceframe(29{\sim}10\;8m),\;portal\;frame(39{\sim}55m),\;domical\;space\;truss(44{\sim}222m),\;framed\;\;membrane(45{\sim}110m),\;hybrid\;\;membrane\;(42{\sim}256m)$ 7) tension forces: cable, membrane, $suspension(60{\sim}150m),\;cable\;\;beam(40{\sim}130m),\;tensile\;membrane(42{\sim}136m),\;cable\;-slayed(25{\sim}90m),\;suspension\;membrane(24{\sim}97m),\;single\;layer\;pneumatic\;structure(45{\sim}231m),\;double\;layer\;pneumatic\;structures(30{\sim}44m)$