• Title/Summary/Keyword: homogeneous

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Homogeneous Enzyme-Linked Binding Assay Mediated by the Interaction of Avidin with Biotin: Mistletoe Lectin I Assay

  • Rhee Paeng, In-Suk;Lee, Eun-Ah;Kim, Hyun-Sook
    • Bulletin of the Korean Chemical Society
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    • v.25 no.1
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    • pp.115-118
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    • 2004
  • We have examined the feasibility of using the specific interaction between mistletoe lectin I (ML I) and ${\beta}$-Dgalactose instead of the anti-ML I antibody in developing a homogeneous type competitive binding assay for ML I. We also have examined the feasibility of adapting the biotin/avidin mediated homogeneous assay for this system. Alkaline phosphatase (AKP) was employed as a single substrate enzyme label. The dose-response curve shows a detection range of 1-25 ${\mu}$g/mL and a linear response with a correlation coefficient of 0.99. To demonstrate the analytical utility of this method, 10 ${\mu}$g/mL of ML I was spiked into distilled water. The results show that the mean recovery was 10.03 ${\mu}$g/mL with an SD of 0.18. The difference between the spiked value and the mean recovery was 0.03 ${\mu}$g/mL, with a relative error of 0.3 and 1.6 % of RSD.

EFFICIENT IMAGE SEGMENTATION FOR MANIFESTING VISUAL OBJECTS

  • Park, Hyun-Sang;Lim, Jung-Eun;Ra, Jong-Beom
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 1999.06a
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    • pp.159-164
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    • 1999
  • Homogeneous but distinct visual objects having low-contrast boundaries are usually merged in most of the segmentation algorithms. To alleviate this problem, an efficient image segmentation algorithm based on a bottom-up approach is proposed by using spatial domain information only. For initial image segmentation, we adopt an efficient marker extraction algorithm conforming to the human visual system. Then, two region-merging algorithms are successively applied so that homogeneous visual objects can be represented as simple as possible without destroying low-contrast real boundaries among them. The resultant segmentation describes homogeneous visual objects with few regions while preserving semantic object shapes well. Finally, a size-based region decision procedure may be applied to represent complex visual objects simpler, if their precise semantic contents are not necessary. Experimental results show that the proposed image segmentation algorithm represents homogeneous visual objects with a few regions and describes complex visual objects with a marginal number of regions with well-preserved semantic object shapes.

Effect of EGR and Supercharging on the Diesel HCCI Combustion (디젤 예혼합 압축착화 엔진에서 배기가스 재순환과 과급의 영향)

  • Park, Se-Ik;Kook, Sang-Hoon;Bae, Choong-Sik;Kim, Jang-Heon
    • Transactions of the Korean Society of Automotive Engineers
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    • v.14 no.5
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    • pp.58-64
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    • 2006
  • Homogeneous charge compression ignition(HCCI) combustion is an advanced technique for reducing the hazardous nitrogen oxide(NOx) and particulate matter(PM) in a diesel engine. NOx could be reduced by achieving lean homogeneous mixture resulting in combustion temperature. PM could be also reduced by eliminating fuel-rich zones which exist in conventional diesel combustion. However previous researches have reported that power-output of HCCI engine is limited by the high intensive knock and misfiring. In an attempt to extend the upper load limit for HCCI operation, supercharging in combination with Exhaust Gas Recirculation(EGR) has been applied: supercharging to increase the power density and EGR to control the combustion phase. The test was performed in a single cylinder engine operated at 1200 rpm. Boost pressures of 1.1 and 1.2 bar were applied. High EGR rates up to 45% were supplied. Most of fuel was injected at early timing to make homogeneous mixture. Small amount of fuel injection was followed near TDC to assist ignition. Results showed increasing boost pressure resulted in much higher power-output. Optimal EGR rate influenced by longer ignition delay and charge dilution simultaneously was observed.

A Technique for Improving the Quality of Stereo DEM Using Texture Filters

  • Kim, Kwang-Eun
    • Korean Journal of Remote Sensing
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    • v.18 no.3
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    • pp.181-186
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    • 2002
  • One of the most important procedure in stereo DEM generation is the stereo matching process which finds the conjugate pixels in a pair of stereo imagery. In order to be found as conjugate pixels, the pixels should have distinct spatial feature to be distinguished from other pixels. However, in the homogeneous areas such as water covered or forest canopied areas, it is very difficult to find the conjugate pixels due to the lack of distinct spatial feature. Most of erroneous elevation values in the stereo DEM are produced in those homogeneous areas. This paper presents a simple method for improving the quality of stereo DEM utilizing the texture filters. An entropy filter was applied to one of the input stereo imagery to extract very homogeneous areas before stereo matching process. Those extracted homogeneous areas were excluded from being candidates for stereo matching process. Also a statistical texture filter was applied to the generated elevation values before the interpolation process was applied in odor to remove the remaining anomalous elevation values. Stereo pair of SPOT level 1B panchromatic imagery were used for the experiments. The results showed that by utilizing the texture filters as a pre and a post processor of stereo matching process, the quality of the stereo DEM could be dramatically improved.

An analytical solution for finitely long hollow cylinder subjected to torsional impact

  • Wang, X.;Wang, X.Y.;Hao, W.H.
    • Structural Engineering and Mechanics
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    • v.19 no.3
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    • pp.281-295
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    • 2005
  • An analytical method is presented to solve the elastodynamic problem of finitely long hollow cylinder subjected to torsional impact often occurs in engineering mechanics. The analytical solution is composed of a solution of quasi-static equation satisfied with the non-homogeneous boundary condition and a solution of dynamic equation satisfied with homogeneous boundary condition. The quasi-static solution is obtained directly by solving the quasi-static equation satisfied with the non-homogeneous boundary condition. The solution of the non-homogeneous dynamic equation is obtained by means of finite Hankel transform on the radial variable, r, Laplace transform on time variable, t, and finite Fourier transform on axial variable, z. Thus, the solution for finitely long, hollow cylinder subjected to torsion impact is obtained. In the calculating examples, the response histories and distributions of shear stress in the finitely long hollow cylinder subjected to an exponential decay torsion load are obtained, and the results have been analyzed and discussed. Finally, a dynamic finite element for the same problem is carried out by using ABAQUS finite element analysis. Comparing the analytical solution with the finite element solution, it can be found that two kinds of results obtained by means of two different methods agree well. Therefore, it is further concluded that the analytical method and computing process presented in the paper are effective and accurate.

A Three-dimensional Spectral Model for the Computation of Wind-induced Flows in a Homogeneous Shelf Sea (취송류 재현을 위한 3차원 스펙트랄모형 개발)

  • So, Jae-Kwi;Jung, Kyung-Tae;Lee, Kwang-Soo;Seung, Young-Ho
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.4 no.2
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    • pp.91-107
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    • 1992
  • A numerical formulation is developed to solve the linear three-dimensional hydrodynamic equations which describes wind induced flows in a homogeneous shelf sea. The hydmdynamic equations are at the outset separated into two systems. namely, an equation containing the gradient of sea surface elevation and the mean flow (external mode) and an equation describing the deviation from the mean flow (internal mode). The Galerkin method is then applied to the internal mode equation. The eigenvalues are determined from the eigenvalue problem involving the vertical eddy viscosity subject to a homogeneous boundary condition at the surface and a sheared boundary condition at the sea bed. The model is tested in a one-dimensional channel with uniform depth under a steady, uniform wind. The analytical velocity profile by Cooper and Pearce (1977) using a constant vertical eddy viscosity in channels of infinite and finite length is chosen as a benchmark solution. The model is also tested in a homogeneous, rectangular basin with constant depth under a steady, uniform wind field (the Heaps' Basin of the North Sea scale).

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Experimental and Numerical Study on the Capillary Performance of Non-Homogeneous Micro-Post Arrays (비 균일적 마이크로 원기둥 배열을 이용한 고성능 냉각 표면)

  • Byon, Chan;Kim, Sung Jin
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.12
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    • pp.1091-1097
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    • 2013
  • In this article, an advanced cooling surface based on micro-post arrays with non-homogeneous configurations is investigated and compared with conventional micro-post arrays with homogeneous configuration. The capillary performance of micro-post arrays are characterized using the capillary rate of rise experiments and numerical simulations which take into account the meniscus curvature. The experimental and numerical results show that that the capillary performance of the micro-post wick can be significantly enhanced, compared with the homogeneous type wick, by employing non-homogeneous configurations. The capillary performance is shown to be primarily a function of the solid fraction and increases linearly with decreasing solid fraction, regardless of the wick configuration, when the solid fraction is larger than 0.25. However, the capillary performance is found to be significantly reduced when the solid fraction falls below approximately 0.25.

Estimation of Design Rainfall by the Regional Frequency Analysis using Higher Probability Weighted Moments and GIS Techniques(I) (고차확률가중모멘트법에 의한 지역화빈도분석과 GIS기법에 의한 설계강우량 추정(I) -동질성의 지역구분 방법을 중심으로-)

  • 이순혁;박종화;류경식;지호근;전택기;신용희
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.43 no.4
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    • pp.57-68
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    • 2001
  • It is matter of common knowledge to give impetus to the water resources development to cope with increasing demand and supply for the water utilization project including agricultural living and industrial water owing to the economic and civilization development in recent years. Regional design rainfall is necessary or the design of the dam reservoir levee and drainage facilities for the development of various kinds of essential waters including agricultural water. For the estimation of the regional design rainfall classification of the climatologically an geographically homogeneous regions should be preceded preferentially This study was mainly conducted to derive the optimal regionalization of the precipitation data which can be classified by the climatologically and geographically homogeneous regions all over the regions except Cheju and Wulreung islands in Korea. A total of 65 rain gauges were used to regional analysis of precipitation. Annual maximum series for the consecutive durations of 1, 3, 6, 12, 24, 36, 48 and 72hr were used for various statistical analysis. Both K-means clustering and mean annual precipitation methods are used to identify homogeneous regions all over the regions. Nine and five homogeneous regions for the precipitation were classified by the K-means clustering and mean annual methods, respectively. Finally, Five homogeneous regions were established by the trial and error method with homogeneity test using statistics of $\chi$$^2$ distribution.

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The effect of soil heterogeneity and container length on the growth of Populus euramericana in a greenhouse study

  • Rahman, Afroja;Meng, Loth;Han, Si Ho;Seo, Gi Chun;Jung, Mun Ho;Park, Byung Bae
    • Korean Journal of Agricultural Science
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    • v.45 no.2
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    • pp.143-153
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    • 2018
  • Soil characteristics along with various container lengths have an important role in the early survival rate and growth of seedlings by influencing the seedling quality. This experiment was conducted to investigate the effect of container length and different soil mixtures on the growth of poplar in a greenhouse. Two types of soil, homogeneous vs. heterogeneous, were used along with two container lengths (30 vs. 60 cm). The heterogeneous soil was made by dividing 50% vermiculite from a mixture of 25% vermicompost and 25% nursery soil in volume. For the homogeneous soil, the above three soil types were mixed together. Populus euramericana clone cuttings were planted in late April, and then, the growth height, root collar diameter (RCD) and biomass were measured in August. The height of the poplar was not significantly affected by container length and soil type, but the RCD was significantly affected by soil type. Leaf and root biomass was higher at the long container than at the short container for both soil treatments, but stem biomass was lower at the heterogeneous soil than at the homogeneous soil treatment. Root to shoot biomass ratio was higher at the heterogeneous soil treatment than at the homogeneous soil treatment by 12%. In conclusion, heterogeneous soil along with a long container is suitable to increase the carbon allocation into the root.

Assessing applicability of self-organizing map for regional rainfall frequency analysis in South Korea (Self-organizing map을 이용한 강우 지역빈도해석의 지역구분 및 적용성 검토)

  • Ahn, Hyunjun;Shin, Ju-Young;Jeong, Changsam;Heo, Jun-Haeng
    • Journal of Korea Water Resources Association
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    • v.51 no.5
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    • pp.383-393
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    • 2018
  • The regional frequency analysis is the method which uses not only sample of target station but also sample of neighborhood stations in which are classified as hydrological homogeneous regions. Consequently, identification of homogeneous regions is a very important process in regional frequency analysis. In this study, homogeneous regions for regional frequency analysis of precipitation were identified by the self-organizing map (SOM) which is one of the artificial neural network. Geographical information and hourly rainfall data set were used in order to perform the SOM. Quantization error and topographic error were computed for identifying the optimal SOM map. As a result, the SOM model organized by $7{\times}6$ array with 42 nodes was selected and the selected stations were classified into 6 clusters for rainfall regional frequency analysis. According to results of the heterogeneity measure, all 6 clusters were identified as homogeneous regions and showed more homogeneous regions compared with the result of previous study.