Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Homogeneous Enzyme-Linked Binding Assay Mediated by the Interaction of Avidin with Biotin: Mistletoe Lectin I Assay

  • Published : 2004.01.20
Download PDF
⟨ Previous Next ⟩

Abstract

We have examined the feasibility of using the specific interaction between mistletoe lectin I (ML I) and ${\beta}$-Dgalactose instead of the anti-ML I antibody in developing a homogeneous type competitive binding assay for ML I. We also have examined the feasibility of adapting the biotin/avidin mediated homogeneous assay for this system. Alkaline phosphatase (AKP) was employed as a single substrate enzyme label. The dose-response curve shows a detection range of 1-25 ${\mu}$g/mL and a linear response with a correlation coefficient of 0.99. To demonstrate the analytical utility of this method, 10 ${\mu}$g/mL of ML I was spiked into distilled water. The results show that the mean recovery was 10.03 ${\mu}$g/mL with an SD of 0.18. The difference between the spiked value and the mean recovery was 0.03 ${\mu}$g/mL, with a relative error of 0.3 and 1.6 % of RSD.

Keywords

References

  1. Jaggy, C.; Musielski, H.; Urech, K.; Schaller, G. Arzneim.-Forsch.Drug Res. 1995, 45, 905.
  2. Franz, H. Advances in Lectin Research; Franz, H. Ed.; VerlagGesundheit and Springer Verlag: Berlin, 1991; Vol. 4, p 33.
  3. Hajto, T.; Hostanska, K.; Fornalski, M.; Kirsch, A. Dtsch. Zschr.Onkol. 1991, 23, 1.
  4. Stein, G. M.; Edlund, U.; Pfuller, U.; Bussing, A.; Schietzel, M.Anticancer Res. 1999, 19, 3907.
  5. Eck, J.; Langer, M.; Mockel, B.; Wilthohn, K.; Zinke, H.; Lentzen,H. Eur. J. Biochem. 1999, 265, 788. https://doi.org/10.1046/j.1432-1327.1999.00784.x
  6. Eschenburg, S.; Krauspenhaar, R.; Mikhailov, A.; Stoeva, S.;Betzel, C.; Voelter, W. Biochem. Biophys. Res. Comm. 1998, 247,367. https://doi.org/10.1006/bbrc.1998.8760
  7. Soler, M. H.; Stoeva, S.; Voelter, W. Biochem. Biophys. Res.Comm. 1998, 246, 596. https://doi.org/10.1006/bbrc.1998.8670
  8. Bussing, A.; Multani, A. S.; Pathak, S.; Pfuller, U.; Schietzel, M.Cancer Letters 1998, 130, 57. https://doi.org/10.1016/S0304-3835(98)00124-4
  9. Ziska, P.; Franz, H.; In Lectins; Bog-Hansen, T. C.; Breboriowicz,J., Eds.; Walter de Gruyter; Berlin-New York, 1985; Vol. 4,p 473.
  10. Vang, O.; Pil Larsen, K.; Bog-Hansen, T. C.; In Lectins; Bog-Hansen, T. C.; van Driesche, E., Eds.; Walter de Gruyter: Berlin-New York, 1986; Vol. 5, p 637.
  11. The Immunoassay Handbook, 2nd ed.; David, W., Ed.; NaturePublishing group: U.K. 2001.
  12. Kim, M. J.; Kim, H. J.; Kim, J. M.; Kim, S. H.; Cha, G. S. Anal.Biochem. 1995, 231, 400. https://doi.org/10.1006/abio.1995.9980
  13. Shim, Y. N.; Paeng, I. R. Bull. Korean Chem. Soc. 2003, 24, 70. https://doi.org/10.5012/bkcs.2003.24.1.070
  14. Choi, M. H.; Kim, M. W.; Cho, H. C.; Kim, M. S.; Lee, E. A.;Paeng, I. R.; Cha, G. S. Bull. Korean Chem. Soc. 2001, 22, 417.
  15. Kjellstrom, T. L.; Bachas, L. G. Anal. Chem. 1989, 61, 1728. https://doi.org/10.1021/ac00190a027
  16. Cha, G. S.; Meyerhoff, M. E. Anal. Chim. Acta 1988, 208, 31. https://doi.org/10.1016/S0003-2670(00)80733-3
  17. Bachas, L. G.; Meyerhoff, M. E. Anal. Biochem. 1986, 156, 223. https://doi.org/10.1016/0003-2697(86)90177-6
  18. Beuth, J.; Ko, H. L.; Tunggal, L.; Geisel, J.; Pulverer, G. Arzneim.-Forsch. Drug Res. 1993, 43, 2.

Cited by

  1. Anti-influenza virus principles from Muehlenbeckia hastulata vol.64, pp.2, 2010, https://doi.org/10.1007/s11418-009-0386-9
  2. Bioluminescence Single-Site Immunometric Assay for Methamphetamine Using the Photoprotein Aequorin as a Label vol.27, pp.3, 2006, https://doi.org/10.5012/bkcs.2006.27.3.407
  3. Comparison of three distinct ELLA protocols for determination of apparent affinity constants between Con A and glycoproteins vol.94, pp.None, 2012, https://doi.org/10.1016/j.colsurfb.2012.01.036