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Antitumor Effect and Immunology Activity of Seaweeds toward Sarcoma-180 (청각과 김에서 추출한 당단백질의 Sarcoma-180에 대한 항암효과 및 면역활성)

  • CHO Kyung-Ja;LEE Young-Suk;RYU Beung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.5
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    • pp.345-352
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    • 1990
  • This study was investigated on the antitumor of protein-polysaccharide fraction(PPF) extracted from seaweeds such as sea-staghorn and laver toward sarcoma-180 cells. In the PPF extracted from these sewaweeds, the polysaccharide contents of sea-staghorn and laver were $62.26\%$ and $65.78\%$, respectively. The highest levels of polysaccharides found in seaweeds was fructose. The major amino acids were aspartic acid, glutamic acid, glycine and cystein. The solid tumor growth inhibition showed the highest level of $53.30\%$ when 50mg/kg sea-staghorn was administrated. The life prolongation effect was $17.35\%$ at 50 mg/kg of laver. In the effects of immunologic activity, when 100mg/kg sea-staghorn was administrated, the number of circulating leucocyte showed the highest level of $82.23\%$ but decreased leucocyte for prolonged times. The number of total peritoneal exudate cells of the sea-staghorn administerated group was increased significantly in comparison with the control group. The hematobiolgoical analysis of the experimental group was similar with that of the control group. This experiments indicated that hemeastasis still maintained nor-mal state and not showed any harmful effects in normal mice.

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Studies on the carotenoids in the viscera of abalone (Haliotis discus hannai) (전복(Haliotis discus hannai) 내장(內臟)의 Carotenoid에 관(關)한 연구(硏究))

  • Ahn, Seung-Yo
    • Applied Biological Chemistry
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    • v.17 no.4
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    • pp.257-274
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    • 1974
  • The studies on the carotenoids in the viscera of abalone (Haliotis discus hannai) have been-carried out. The pigments were extracted with acetone-methanol mixture (4 : 1) from the viscera of abalones which were caught around the coastal water of Korea from March to August. The individual carotenoid in the extracts was separated and purified by the silica gel TLC, $Mg(OH)_2$ impregnated paper chromatography and $Mg_2(OH)_2CO_3$ TLC. The isolated eleven carotenoids were investigated and identified by epoxide test, partition test, reduction with sodium borohydride, alkaline hydrolysis, co-chromatography and comparative test with reference carotenoids and electronic and IR absorption spectrophotometry. ${\alpha}$-Carotene, ${\beta}$-carotene. lutein, zeaxanthin, siponaxanthin, siponein, fucoxanthin, loroxan-thin-like and fucoxanthinol-like have been identified among the eleven carotenoids isolated. It has been found that fucoxanthin, on alkaline treatment, was transformed to the product of which chromophore was the same one as fucochrome and semifucoxanthol. Among the identified nein carotenoids siphonaxanthin, siponein, fucoxanthin, loroxanthin-like and fucoxanthinol-like have not been reported previously to be contained in the shellfish.

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Cooking and Sorption Characteristics of Black Soybeans (검정콩의 조리(調理) 및 흡습성질(吸濕性質))

  • Shin, Ea-Sook;Kim, Chong-Kun;Zong, Moon-Shik;Kim, Woo-Jung
    • Applied Biological Chemistry
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    • v.28 no.2
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    • pp.51-55
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    • 1985
  • Dried black soybeans were soaked in water at the temperature range of $4^{\circ}C{\sim}100^{\circ}C$ and in salt or sugar solution at $100^{\circ}C$ in order to investigate their effects on the cooking ratio and diffusion property of color pigments. As the temperature increased, the equilibrated absorbance at 490nm of soaking water after 120min was linearly increased. The activation energy for diffusion of color pigments were found to be 4.23 Kcal/mole at the range of $60^{\circ}C{\sim}100^{\circ}C$ and 8.31 kca1/mo1e at $4^{\circ}C{\sim}60^{\circ}C$. The cooking ratio, % cooked beans after heating, of black soybeans were more affected by the salt concentration that of sugar. Both concentrations reduced the cooking ratio as they increased. The sorption isotherm of Kongjaban showed a quite different curves between the storage at $4^{\circ}C$ and $25^{\circ}C$. The sorption and desorption rates during storage could be represented by the equation of $log({\frac{dw}{dt}}{\times}10^3))=alogt+logb$ with a very high correlation.

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Effects of Aloe on Liver Function and Lipid Metabolism in Alcohol-Consuming Rats (Aloe가 알코올을 섭취한 흰쥐의 간 기능 및 지질대사에 미치는 영향)

  • Choi, Hye-Gyoung;Lee, Joon-Ho
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.3
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    • pp.325-334
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    • 2014
  • The effects of aloe on liver function and lipid metabolic disorders induced by alcohol consumption were studied in rats using aloe power (0.25%, 0.5%, 1%) and 10% ethanol. 35 Sprague-Dawley (male, 4 weeks old) rats were divided into five groups and fed experimental diets for six weeks. Body weights of rats tended to be lower in all alcohol supplemented groups than in the control. Food intakes and dry feces per day were significantly lower in all alcohol supplemented groups than in the control. Atherogenic indices (AI) were highest in the alcohol group and decreased in proportion with aloe amount. Serum triglyceride level was significantly higher in the alcohol group than in the control, but tended to be lower in the aloe supplemented groups. In relation to liver function, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), and alkaline phosphatase (ALP) activities tended to be higher in the alcohol groups than in the control, but lower in the aloe groups, especially in the alcohol+0.5% AO group. The levels of liver cholesterol were significantly lower in the alcohol group than in the control and aloe supplemented groups. In the histochemical evaluation, fat droplets appeared extensively on the liver-lobule in the alcohol group, whereas they decreased slightly in the alcohol+0.25% AO group and apparently disappeared in the alcohol +0.5% AO. On the other hand, fat droplets appeared again on the liver-lobule in the alcohol+1% AO group, but were reduced compared with the alcohol group. Regarding the fatty acid composition of adipose tissue triglycerides, the level of linoleic acid (18:2) was significantly higher in the aloe supplemented group. Regarding the fatty acid composition of liver phosphatidylcholine (PC), the level of linoleic acid was higher in the alcohol group and alcohol+1% AO group than the other groups. In contrast, the level of arachidonic acid was significantly lower in the alcohol group. As a result, arachidonic / linoleic acid ratios were significantly lower in the alcohol group compared to the control group, whereas the ratios of the aloe supplemented groups were similar to that of the control group. Therefore, aloe had some beneficial effects on lipid metabolic disorders induced by alcohol and affected desaturation of fatty acids.

A Study on Elementary School Dietitian's Status and Recognition of Nutrition Education in Incheon (인천지역 초등학교 영양사의 영양교육 실태 및 인식에 관한 연구)

  • Park Jeong Ah;Chang Kyung Ja
    • Korean Journal of Community Nutrition
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    • v.9 no.6
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    • pp.716-724
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    • 2004
  • The purpose of this study was to investigate the elementary school dietitian's status and recognition of nutrition education (NE) in Incheon. A cross-sectional study was carried out using a self-administered questionnaire and subjects were 100 elementary school dietitians. The results are as follows. As for training in NE, $61.2\%$ of the dietitians attended training in NE. After training in NE, $86.5\%$ of the dietitians who attended training in NE were more concerned about NE. Also $59.5\%$ of the dietitians gave students NE and most of them did as a weekly printout 2-4 times per month. There was a significant difference in experience of NE for teachers between subgroups by experience of training in NE; while $48.1\%$ of the dietitians with training in NE gave teachers NE, $20.0\%$ of the dietitians without training in NE gave teachers NE. The main reason for not giving NE was too much work load and low concern of school administration. Also $96.4\%$ of the dietitians answered that NE is necessary in elementary school and the main reason for being necessary was correction of unbalanced diet and good table manner, As for proper time to start NE for children, $51.8\%$ of dietitians answered 'kindergarten' and $45.8\%$ of them answered 'lower grade of elementary school'. As for effective type for NE, $59.5\%$ of the dietitians answered 'NE as a part of other subject' and $23.8\%$ of them answered 'NE as a separate subject'. Also $79.5\%$ of the dietitians answered 'teacher' as the suitable person for NE. Most of the dietitians recognized menu formation as the ideal major work load and office work as the most time-consuming work load. As to job satisfaction, most of them were dissatisfied with office work and NE. Therefore, it is nationally supported for elementary school students' health and well-being that school dietitians as NE specialists give NE with minimizing their office work and developing a standardized NE program.

DENTAL TREATMENT IN A PATIENT WITH METACHROMATIC LEUKODYSTROPHY UNDER GENERAL ANESTHESIA : A CASE REPORT (이염성 백질 이영양증 환아의 치아우식 치료 증례 보고)

  • Ryu, Jiyeon;Shin, Teo Jeon;Hyun, Hong-Keun;Kim, Young Jae;Kim, Jung-Wook;Jang, Ki-Taeg;Kim, Chong-Chul;Lee, Sang-Hoon
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.12 no.2
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    • pp.96-100
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    • 2016
  • Metachromatic leukodystrophy (MLD) is a progressive and degenerative neurological disease caused by a deficiency of the catabolic enzyme arylsulfatase A. Deficiency of arylsulfatase A results in accumulation of sulfatide in the white matter of the peripheral and central nervous system and it occurs demyelination as a result. The patient gradually goes through mental and motor failure. General symptoms of MLD include gait disturbance, mental deterioration, muscle rigidity and impaired swallowing. Inheritance of the disease is autosomal recessive. We report a dental caries treatment of a 3-year old boy with MLD. The patient underwent hematopoietic stem cell transplantation (HSCT) to slow the progression of the disease. He was suffered from difficulties of mastication and swallowing from the degenerative neurological symptom. He was ingesting food by both oral feeding and tubal feeding after he took percutaneous endoscopic gastrostomy (PEG). The cause of multiple caries was mainly presumed as patient's prolonged time of meal. The treatment was performed under general anesthesia considering patient's incompliance. Severely affected lower primary molars were treated with pulp treatment and restored with stainless steel crown. Others were restored with composite resin. There were no postoperative complications. MLD is life threatening progressive disease and also has an impact on unfavorable condition for oral health. Routine home oral care and periodic professional dental care should be emphasized to the caregiver of patient considering the susceptibility of dental caries. Not only the medical care, but periodic dental office visit would benefit the quality of life of the patient.

An Experience of Korean Consumer's Monitoring on Nanoproducts (국내 나노제품에 대한 시민 모니터링 결과 고찰)

  • Kim, Hoon-Gi
    • Journal of the Korean Vacuum Society
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    • v.19 no.6
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    • pp.442-452
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    • 2010
  • Our team carried out a new program for public engagement on nanotechnology in Korea. We chose 22 monitors, who majored in science or technology and graduated from universities long time ago. Most of them were married and housewives. This 'focus group' had not only general knowledge about science or technology but also much interest in social activities. The 167 nanoproducts to be monitored were for daily life, e.g. home appliances (washing machine, refrigerator, water purifier, etc), clothing, cosmetics, food, toy, and others. And the period of it was one month. The monitors had a sheet with 10 questions, and filled them out in essay form. All of them submitted 2~3 sheets every weekend to our team. Before monitoring, our team had a meeting for introduction and explanation about the potential risk of nanotechnology as well as benefits from it. Another meeting was held after finishing monitoring to share their experience one another. The main results of the monitoring were as follows: the number of nanoproducts describing both the definition of 'nano' and the size of nanomaterials was just 2 (1.2%) the number of them explaining the technical methods enough was 15 (9/0%) the number of them accounting for the reason of functional improvement enough was 14(8.4%); the number of them doubtful as if there would be exaggeration or false knowledge was 27 (16.2%); the number of them commenting potential hazards to human health or environment was almost zero; the number of them describing about safety certification acceptable was 9 (5.4%). The monitors made a proposal containing recommendation to Government and industry. The contents were as follows: industry should make the manual in detail and correctly, Describe Certificate detailed and correctly, Do research on risk and toxicity continually, Educate employee about nanoproducts at consumer's center; Government should make indication of nanoproducts compulsory, Appoint Certificate Authority and make Certificate Mark guaranteeing the safety on nanoproducts, Make detailed explanation about nanoproducts compulsory.

Distribution of Habitats and Ecology of Weedy Melons (Cucumis melo var. agrestis Naud.) in Korea (우리나라 야생잡초 참외의 자생지 분포지역 및 생태)

  • Lee, Woo-Sung
    • Horticultural Science & Technology
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    • v.31 no.5
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    • pp.652-655
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    • 2013
  • Natural habitats of weedy melons were distributed on the islands along and on the west and south coasts of Korean peninsula including Boryeong, Seosan (Taean), Seocheon, Okgu, Buan, Gochang, Yeonggwang, Muan, Shinan, Haenam, Jindo, Wando, Goheung, Yeocheon, Hadong, Namhae, Goseong, Tongyeong, Geoje, and Jeju islands including Jeju city, Bukjeju-gun and Nam Jeju-gun. Weedy melons were found growing wildly in or around the cultivated lands in these regions. Natural habitats of weedy melons were in and around the cultivated lands. Weedy melon plants were found most often in soybean fields, followed by fields of mungbean, sweet potato, pepper, sesame, cotton, and peanuts. The plants were also found growing wild in foxtail millet fields, rice paddy levees along the streams, upland field edges, watermelon fields, corn fields, vegetable gardens near farmhouse, orange fields, compost piles, fallow fields, roadside and home gardens. They inhabited in sunny and a little dry spaces in relatively low-height crop plant fields in general. The time of fruit maturity was from early July to late October with the most frequency in September according to post survey answer. Fruits dropped off from the fruit stalk when matured. This phenomenon was thought beneficial for perpetuation in the wild. The fruits were being used commonly for food and toys for children. It was thought that weedy melons were perpetuating through the cycle of human and animal feeding of the fruits, human and animal droppings, often mixed in compost, and application of the compost to crop fields by human.

Ring Test as Acute Toxicity Test with Korean Freshwater Shrimp, Neocaridina denticulata using 3,4-Dichloroaniline (국내 서식 담수새우 새뱅이(Neocaridina denticulata)를 이용한 3,4-Dichloroaniline의 급성독성 교차시험(Ring test))

  • Shin, Yu-jin;Lee, Jae-woo;Kim, Jieun;Cho, Jaegu;Kim, Ja-Hyun;Kang, Minho;Kim, Kyungtae;Kim, Pil-je;Park, Kyunghwa
    • Journal of Environmental Health Sciences
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    • v.45 no.3
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    • pp.285-294
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    • 2019
  • Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.

Analyzing adolescent family meal vs. alone meal: Focusing on adolescent time use and family characteristics (청소년의 가족식사와 혼밥 비교분석: 청소년의 시간활용과 가족특성을 중심으로)

  • Cha, Seung-Eun;Lee, Hyun Ah
    • Journal of Family Relations
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    • v.22 no.4
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    • pp.135-156
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    • 2018
  • Objectives: This study aim to determine adolescent meal time, which provides the important context of parent-child sharing time. We divided mealtime into family meal and alone meal, and analyzed the time/space context of each meal time as well as the social determinations. Method: We employed adolescents age 10-18 who live with married patents and attends school at the present (n=5,128) from the original data of Korean Time Use Survey. Results: More adolescent were engaged in family meal rather than eating alone in daily bases. However, the trend show difference by day of week and academic grade; family meal are more prevalent in weekends rather than weekdays. As adolescent reaches high school age, the proportion of eating alone beats the proportion of family meal time. Most of the meal occur at home. Having meal outside was relatively scares, especially on weekdays. Tobit and logistic analysis reveal that, on weekday meal, less school hours, more time spent at the private academy, having family leisure event, and long mother's housework hours were positively associated with family meal time. In weekend model, father's education gradient was associated with family meal time, showing higher the father's education level, there were higher chance of having weekend family meal. As for the eating alone, relevant factors were similar with family meal but the directions were the opposite; having family leisure were negatively associated with alone meal, both weekdays and weekend. Long academy hours, meal preparing and leisure alone were positively associated eating alone. Overall, weekday meal time was strongly linked with adolescent daily schedules and time use, while for weekends meal, in both family meal and alone meal, the influence of parent factors were discovered. Conclusion: The results indicates that alone meal and the family meal are not exclusively related but seem to be complementary. Families tend to enjoy family meal yet, there are some necessary situation that adolescent need to be on their own. Increase in ready-made food industries, growing independence of children by age seem partly allow adolescent children to eat alone. Careful attentions may require for monitoring weekends meal situation and the family factor of adolescent in future studies.