The modified simultaneous differential staining technique, which enables double staining of cartilage and bones, needs to be improved to prevent soft tissues from being damaged during the staining process. Key factors influencing the extent to which soft tissues are damaged include the fixative used, macerating time, potassium hydroxide concentration, incubation temperature and the removal of skin from specimens. Here we describe a protocol that enables the hardening of tissues during bleaching and maceration. We also describe a method for objectively measuring rates of cartilage and bone growth. The use of formalin as a fixative rendered soft tissues more rigid due to the resulting chemical bonds formed between proteins. Blotted specimens were immersed in 1% potassium hydroxide (KOH) and incubated at $37^{\circ}C$ for 1 day (smaller specimens) or 2-3 days (larger specimens). The 1% KOH solution was also used as the diluent solution for the subsequent immersion in a graded series of 30%, 50%, 70%, 90%, 100% glycerol solutions, a procedure that made soft tissues even more transparent and hardened. It was not necessary to remove the skin of specimens shorter than 2 cm, since the macerating solution could easily penetrate their thin skin layer and continuously remove those pigments hindering visibility. Since excessive osmosis is another factor that can damage soft tissues in the macerating process by causing the rupture of those cells not able to withstand the osmotic pressure, here it was minimized by balancing the salt concentration between the interior and exterior of cells with the addition of 0.9% sodium chloride (NaCl) in the macerating solution. Finally, to determine the proportions of cartilage and bone growth, photographs of the stained specimens were taken with a dissecting microscope and sections corresponding to the cartilage and bones were cut out from the printed pictures and weighed. Our results show that this method is suitable for the objective evaluation of bone and cartilage growth.
Journal of The Korean Association of Information Education
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제8권2호
/
pp.227-238
/
2004
While the first step of the comprehensive plan for educational informationization focused on the infrastructure establishment, the second step aims to develop creativity and problem solving skills of students through the use of Information and Communication Technology(ICT). The purpose of this research is to investigate the current status of the educational use of ICT in elementary schools, and to give guidelines for future educational informationization. Four aspects of ICT(i.e., school environments, teachers' abilities to use ICT, methods for using ICT in class, and factors hindering the use of ICT in schools) were examined in the survey. From the survey, it is found that ICT is now used in schools more actively than before. However, it is also found that more efforts need to be made in some areas such as instructional strategies using ICT in class, and the design and the management of teacher training programs.
Journal of The Korean Association of Information Education
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제4권1호
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pp.83-97
/
2000
The utilization of Information and Communication Technology(ICT) has become increasingly important in educational endeavors. The purpose of this paper is to analyze the current status of the utilization of ICT in elementary schools and to investigate problems and tasks to be solved for the effective use of ICT. Concerning the first purpose, a survey was conducted in February 2000, and the results are reported in this paper. For the second purpose, hindering factors are analyzed in relation to hardware, software, teacher training, curriculum, and administrative and financial support. Undoubtedly, ICT has been playing an important role in education. The effectiveness of this role largely depends upon how ICT is utilized in educational setting. Thus educators should carefully consider all aspects of the implementation of ICT, and devote efforts to solve problems occurring in the process of the implementation.
Purpose Among various psychosocial factors, negative social comparison, attention blocking to stimuli, and cognitive interference via information overload are considered most critical in hindering the effective performance improvement of computer-based idea generation. Given that the effect of negative social comparison along with a plausible solution based on the notion of performance feedback and goal setting has been successfully addressed, this study focused on the remaining issues of "attention blocking to stimuli and cognitive interference via information overload" and attempted to find a way to alleviate the effect of such process losses on performance. Design/methodology/approach A 2 × 4 between-subjects design was used, crossing cognitive ability (high and low) and personality (extroversion and introversion). Five subjects per each treatment were randomly selected to make the sample size equal. The group simulator was used to measure individual-level performance. The dependent variables were the quantity of and quality score of ideas. The manner by which these performance measures were operationalized was consistent with prior studies. An additional analysis using the number of diverse ideas was also conducted. Findings Three arguments were made in this study: (1) high cognitive individuals would perform better than low cognitive individuals, (2) extraverted individuals would perform better than introverted individuals, and (3) cognitive ability and personality would interact such that individuals in Q1 would have the highest performance. Cognitive ability had an effect on quality not quantity. Personality had an effect on both quantity and quality. An interaction between cognitive ability and personality was not found due to small sample size despite the use of the group simulator.
The Journal of the Convergence on Culture Technology
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제8권1호
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pp.583-590
/
2022
With the release of the Bitcoin source in 2009, cryptocurrencies are continuously developing and expanding the market. Recently, new applicability is expanding centered on NFT coin and metaverse payment service. In particular, the Central Cryptocurrency Exchange actively supports relay transactions between cryptocurrencies or between traditional fiat currencies and cryptocurrencies. The cryptocurrency trading market based on such a central exchange encouraged speculative factors of cryptocurrencies, strongly arousing speculation and futility of cryptocurrencies. In addition, the central cryptocurrency exchange induces the centralization of users and virtual assets, thereby hindering the decentralization and security enhancement strategies of the block chain. Therefore, this study describes the current status and problems of centrally controlled centralized cryptocurrency exchanges in service, and presents a distributed cryptocurrency exchange modeling strategy and major issues as a decentralization model of the exchange. This research can strengthen the anonymity, decentralization, and autonomy of cryptocurrency based on blockchain.
Kim, Y.S.;Oh, T.K.;Kim, C.S.;Lee, N.E.;Hong, C.S.;Lee, S.Y.;Yoon, Y.G.
Journal of the Korean Society of Safety
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제37권1호
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pp.30-40
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2022
The death rate in the construction industry, which is more than twice that of other industries, increases the need to solve chronic safety problems. In a situation in which the safety management measures that have been maintained thus far are unable to reduce construction accidents, the introduction of smart construction safety technology (SCST) that reflects the 4th industrial technology is required for the safety management of workers. Large corporations or public institutions have introduced SCST and are operating pilot sites, but SCST is still in its infancy and there have yet to exist field data regarding necessity and effectiveness. In this study, the concept of SCST, which has still to be established, is defined, and the actual condition of SCST was analyzed through a survey. Through the analysis of the necessity of SCST introduction, convenience, and effectiveness before and after its application, problems such as problems from the field point of view as well as other factors hindering the introduction of such technology are derived in detail. In addition, an effective combination of technologies for safety management at the current level is determined and an activation plan is presented.
Despite the serious problem of idle communal facilities in rural areas, previous studies focused on determining their status and grasping their current status rather than proposing fundamental solutions. The purpose is to suggest a direction for activating these facilities. To identify the key factors hindering the use of idle and under-utilized facilities in rural areas, a survey was conducted on three groups of interested parties, public officials, operators, and rural residents, to identify items that were effective and examine significant cognitive differences between the groups. Based on the results of the research analysis, it was concluded that although there are differences between the groups in the level of awareness, the causes of preventing facility utilization appear to be similar. Although local governments desire to avoid criticism for idleness, it was found that they are most aware of the limitations of administrative support. Even within the village, residents do not recognize the need for additional facilities unconditionally but do realize their own inadequacies. Thus, despite the differences among stakeholders, it was found that there is a consensus regarding the problem of idle communal facilities in rural areas. As a result, the operating entity is delegated to an individual rather than a joint entity so as to allow the facility to be operated responsibly, and local ordinances are enacted by a group of local officials in order to overcome administrative guidelines limitations. It is suggested that the main issue is securing manpower.
Journal of the Korea Society of Computer and Information
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제29권7호
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pp.81-88
/
2024
Despite the rapid growth of the generative AI market and significant interest from domestic companies and institutions, concerns about the provision of inaccurate information and potential information leaks have emerged as major factors hindering the adoption of generative AI. To address these issues, this paper designs and implements a question-answering system based on the Retrieval-Augmented Generation (RAG) architecture. The proposed method constructs a knowledge database using Korean sentence embeddings and retrieves information relevant to queries through optimized searches, which is then provided to the generative language model. Additionally, it allows users to directly manage the knowledge database to efficiently update changing business information, and it is designed to operate in a private network to reduce the risk of corporate confidential information leakage. This study aims to serve as a useful reference for domestic companies seeking to adopt and utilize generative AI.
Expecting the expansion of the elderly population under long-term home care with the coming of the aged society, this study purposed to propose a prevention and self-reliance support model and to get practical implications for minimizing dependency on care benefits and enhancing the effectiveness of prevention and self-reliance support. Research methods employed for this study were: first, reviewing theoretical literature for clarifying the concept of prevention and self-reliance support in providing long-term care benefits for the elderly; second, identifying factors hindering prevention and self-reliance support through analyzing standard long-term care use plans and documents related to long-term care benefits at elderly welfare centers to which the research subjects belonged; and third, surveying care benefit users on factors hindering their use of prevention and self-reliance support and their needs in the use of care benefits. Based on the results of the three types of qualitative research, we proposed directions for prevention and self-reliance support modeling and suggested practical implications for enhancing the effectiveness of prevention and self-reliance support. For this study, we collected documentary materials and conducted in-depth interviews with the participants with the consents and cooperation of managers and professional social workers at day care centers and elderly welfare centers in D City. According to the results of this study, literature review suggested that long-term care prevention and self-reliance support should be provided in a way of 'strengthening user-centered support systems,' which support elderly long-term care beneficiaries' right to lead a life as the subject of their own life. Document analysis found the absence of benefits related to health and medicine and lack of social support systems for prevention and self-reliance support, and the results of in-depth interviews suggested the necessity to strengthen services related to elderly long-term care beneficiaries' prevention and self-reliance, and the keen needs of the long-term care elders for prevention and self-reliance included: ① loneliness, anxiety, fear; ② missing for and worry about children and people; ③ moving, outing; ④ health and medical services, rehabilitation programs; ⑤ desire to use day care; ⑥ inconvenience of house structure; ⑦desire for meal menus; and ⑧ the occurrence of disuse syndrome. Based on these results, we suggested the base of prevention and self-reliance support modeling with three axes: ① strengthening user-centered support systems; ② strengthening support systems connected to health and medicine; and ③ strengthening social support systems.
Shedding new light on the research trend on entrepreneurial ecosystems in the 40-year history of the Asia Pacific Journal of Small Business, this study aims at exploring a potential measurement framework of ecological inputs and outputs in an entrepreneurial ecosystem that promotes entrepreneurship at geographical and spatial levels. As a result of the analysis of research on the entrepreneurial ecosystem in the journal, we found that prior studies emphasized the managerial importance of various ecological factors on the premise of possible causalities between the factors and entrepreneurship. However, empirical research to verify the premised causality has been underexplored yet. This literature gap may lead to unbalanced development of conceptual and case studies that identify requirements for successful entrepreneurial ecosystems based on experiential facts, thereby hindering the generalization of the research results for practical implications. In that there is a growing interest in creating and operating productive entrepreneurial ecosystems as an innovation engine that drives national and regional economic growth, it is necessary to explore and develop the measurement framework for ecological factors that can be used in future empirical research. Hereupon, we apply a conceptual model of 'input-output-outcome-impact' to categorize individual environmental factors identified in prior studies. Based on the model. We operationalize ecological input factors as the financial, intellectual, institutional, and social capitals, and ecological output factors as the establishment-based, innovation-based, and performance-based entrepreneurship. Also, we propose several longitudinal databases that future empirical research can use in analyzing the potential causality between the ecological input and output factors. The proposed framework of entrepreneurial ecosystems, which focuses on measuring ecological input and output factors, has a high application value for future research that analyzes the causality.
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