• Title/Summary/Keyword: higher pairs

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Eclosion and Mating Behavior of Matsumuraeses phaseoli (Matsumura) (Lepidoptera: Tortricidae) (팥나방(Matsumuraeses phaseoli ) (나비목: 잎말이나방과)의 우화와 교미 행동)

  • Cho, Jum Rae;Jung, Jin Kyo;Yang, Chang Yeol;Seo, Bo Yoon;Yum, Ki Hong
    • Korean journal of applied entomology
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    • v.52 no.3
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    • pp.249-253
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    • 2013
  • The adzuki pod worm, Matsumuraeses phaseoli (Matsumura) is an insect pest that attacks the flowers and pods of Vigna angularis. The circadian rhythms of eclosion and mating, and the effects of age and antenna ablation on mating success, were investigated. With a photoperiodic regime of 16:8 (Light:Dark) h, most adult eclosion was observed within 4 h after lights-on. Most adult mating was observed during the scotophase, although some occurred within 2 h after lights-on. However, the adults did not copulate on the day of emergence. The mating rate increased with adult age: being higher in the 4-d-old adult pairs than pairs of lower age. Most of the antennectomized males or females did not copulate with normal individuals of the opposite sex.

Overexpression of Tbx3 Predicts Poor Prognosis of Patients with Resectable Pancreatic Carcinoma

  • Wang, Hong-Cheng;Meng, Qing-Cai;Shan, Ze-Zhi;Yuan, Zhou;Huang, Xin-Yu
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.4
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    • pp.1397-1401
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    • 2015
  • Background: To determine the expressions of Tbx3, a member of subgroup belonging to T-box family, and its prognostic value in pancreatic carcinoma. Materials and Methods: We determined the expression levels of Tbx3 on both mRNA and protein levels in 30 pairs of fresh tumor tissues and paratumor tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. In addition, protein level of Tbx3 were identified using immunochemistry in 80 pairs of paraffin-embedded specimen. The correlations between Tbx3 expression and various clinicopathological parameters as well as overall survival were evaluated. Results: Tbx3 mRNA and protein levels in tumor tissues were significantly higher than in the paratumor tissues by qRT-PCR ($0.05{\pm}0.007$ vs. $0.087{\pm}0.001$, p<0.001) and western blotting ($1.134{\pm}0.043$ vs. $0.287{\pm}0.017$, p<0.001). The statistical analysis based on immunohistochemical evaluation suggested that Tbx3 aberrant expression was significantly associated with several conventional clinicopathological variables, such as gender, age, tumor position, preoperative CA19-9 level, pathological T staging and N staging. Univariate and multivariate analyses revealed that Tbx3 expression was an independent prognostic factor for overall survival (<0.001). Conclusions: Our results suggest that overexpression of Tbx3 is associated with poor prognosis of pancreatic cancer patients. However, additional clinical trials are needed to accurately validate this observation.

Effect of Crystallographic Orientation on Fracture Mechanism of Ni-Base Superalloy

  • Han, Chang-Suk;Lim, Sang-Yeon
    • Korean Journal of Materials Research
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    • v.25 no.11
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    • pp.630-635
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    • 2015
  • The fatigue strength of a nickel-base superalloy was studied. Stress-controlled fatigue tests were carried out at $700^{\circ}C$ and 5 Hz using triangular wave forms. In this study, two kinds of testing procedures were adopted. One is the conventional tension-zero fatigue test(R = 0). The other was a procedure in which the maximum stress was held at 1000 MPa and the minimum stress was diverse from zero to 1000 MPa at 24 and $700^{\circ}C$. The results of the fatigue tests at $700^{\circ}C$ indicate that the fracture mechanism changed according to both the mean stress and the stress range. At a higher stress range, ${\gamma}^{\prime}$ precipitates are sheared by a/2<110> dislocation pairs coupled by APB. Therefore, in a large stress range, the deformation occurred by shearing of ${\gamma}^{\prime}$ by a/2<110> dislocations, which brought about crystallographic shear fracture. As the stress range was decreased, the fracture mode gradually changed from crystallographic shear fracture to gradual growth of fatigue cracks. At an intermediate stress range, as it became more difficult for a/2<110> dislocation pairs to shear ${\gamma}^{\prime}$ particles, cracks started to propagate in the matrix, avoiding the harder ${\gamma}^{\prime}$ particles. High mean stress induced creep deformation, that is, ${\gamma}^{\prime}$ particles were sheared by {111}<112> slip systems, which led to the formation of stacking faults in the precipitates. Thus, the change in fracture mechanism brought about the inversion of the S-N curves.

Building Extraction and Digital Surface Models Generation from Stereo pairs of Aerial Images (입체 항공사진영상을 이용한 DSM생성 및 건물경계추출)

  • 유환희;김성우;성민규
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.16 no.2
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    • pp.177-185
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    • 1998
  • There is an increasing request for 3D data and outlines on building for urban planning and design. This paper describes an approach to extract building using Digital Surface Models(DSM) and stereo pairs of aerial images. DSM contain informations not only about the topographic surface like Digital Elevation Models(DEM), but also about buildings and other objects higher than the surrounding topographic surface, e.g. tees. We therefore describe our approach consisting of two step procedures. The first step of the approach is to generate DSM by stereo matching using Maximum Likelihood Estimation and Dynamic Programming. The proposed stereo matching is using the cost function for finding the disparity between the left and right image, and the Dynamic Programming for solving the stereo matching problem. The second step is to detect building outlines using the DSM and the edge informations extracted from a digital aerial image by Sobel Operator. The overlay analysis of the DSM and the edge information by Sobel Operator was efficient to detect building outlines.

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Optimization of a microarray for fission yeast

  • Kim, Dong-Uk;Lee, Minho;Han, Sangjo;Nam, Miyoung;Lee, Sol;Lee, Jaewoong;Woo, Jihye;Kim, Dongsup;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • v.17 no.3
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    • pp.28.1-28.9
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    • 2019
  • Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

Effect of SMA on the Interfacial Shear Strength for Single Glass Fiber and PC/SAN Blends (SMA가 PC/SAN 블렌드와 유리섬유간의 계면결합력에 미치는 영향)

  • Lee, Ui-Hwan;Nam, Gi-Jun;Lee, Jae-Uk
    • Polymer(Korea)
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    • v.25 no.4
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    • pp.512-520
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    • 2001
  • One of the most important factors which affect the mechanical properties of fiber-reinforced composite materials is the interfacial shear strength (IFSS). The IFSS of glass fiber and polycarbonate (PC)/styrene-co-acrylonitrile (SAN) blend system has been measured by the single fiber fragmentation test (SFFT). SAN contents were varied up to 30 wt% and the IFSS increased with the SAN contents. Styrene-co-maleic anhydride (SMA) was used as the compatibilizer and the glass fiber was surface treated with organosilane coupling agents. Addition of small amount of SMA in PC/SAN blend improved the IFSS by chemical bonding between maleic anhydride and silanol. The optimum MA content was 0.4 wt% of total matrix contents. Also, IFSS was greatly affected by the miscibility condition of SAN/SMA blends, which depended on the copolymer composition of SAN and SMA. It was found out that, higher IFSS could be obtained when the SAN/SMA blend was in miscible pairs. In case of SAN/SMA miscible pairs, the IFSS depended on the MA content in total matrix, not on the MA content in SMA.

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The Relationship between Maternal Psychosocial Competence, Acceptance and Children's Social Competence (어머니의 사회심리적(社會心理的) 역량(力量) 및 자녀에 대한 수용성(受容性)과 아동의 사회적(社會的) 역량(力量)과의 관계)

  • Park, Ho-Joon;Chong, Young-Sook
    • Korean Journal of Human Ecology
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    • v.2 no.1
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    • pp.1-15
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    • 1993
  • The purpose of this study is to identify what relationship exists between the children's social competence and psychosocial competence of mother, mother's acceptance for her children and is to clarify how children's sex, the demographic backgrounds of the families are affecting for the children's social competence. The detailed research areas which were dealt with in this study can be described as follows :1) Are there any relationships between mother's psychosocial competence and children's social competence? 2) Are there any relationships between mother's acceptance and children's social competence? 3) Are there any differences in children's social competence according to their sex? 4) Are there any differences in children's social competence according to familie's demographic backgrounds? Third grade 314 children who attend primary-school in Chongju were randomly selected as the subjects for the study and their mothers were also involved as subjects. Among them, mother-daughter pairs are 151 and mother-son pairs are 163. The result of this study is as follows: 1. There appeared significant positive correlations between the mothers' psychosocial competence and children's social competence. 2. There appeared significant positive correlations between the maternal acceptance and children's social competance. As the result of a stepwise multiple regression analysis, maternal acceptance appeared as a variable which indicates children's social competence. 3. There appeared differences in competence between boys and girls, and girls' social competence was higher than that of boys. 4. There appeared children's social competence differences according to the socioeconomic status of a family. Among demographic backgrounds of a family, mothers' academic careers, mothers' age, fathers' jobs, income were the variables which affect children's social competence.

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Wavelet Compression Experiments of the Remotely Sensed Images for Three Kinds of Wavelet Families

  • Jin, Hong-Sung;Han, Dong-Yeob
    • Spatial Information Research
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    • v.17 no.4
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    • pp.455-462
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    • 2009
  • A method to find the nearly optimal PSNR values for compression was tried to remotely sensed images. There is no rule to find the best wavelet pairs for image processing. The expected wavelet pairs following the suggested algorithm showed the optimal result for various kinds of images. Firstly, the PSNR variations with three wavelet families were analyzed. In many cases the longer wavelet filter shows the higher PSNR value, but the rate is getting less in orthogonal wavelet families. Wavelets with moderate filter length are suggested at the point of computational cost. For biorthogonal families it was hard to predict from the length of filters. Multiresolution wavelet analysis was used up to level 3 with three kinds of wavelet families. Biorthogonal wavelet family showed irregular pattern to get the maximum PSNR values, while orthogonal wavelet families showed regular pattern. In orthogonal wavelet families the nearly optimal wavelet pair can be predicted from the level 1.

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In vitro evaluation of color and translucency reproduction of maxillofacial prostheses using a computerized system

  • Nemli, Secil Karakoca;Gungor, Merve Bankoglu;Bagkur, Meral;Bal, Bilge Turhan;Arici, Yeliz Kasko
    • The Journal of Advanced Prosthodontics
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    • v.10 no.6
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    • pp.422-429
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    • 2018
  • PURPOSE. Accurate color matching of maxillofacial prostheses to skin is important for esthetics. A computerized color matching system specific to human skin has recently been developed. The purpose of this study was to evaluate the accuracy in color and translucency matching of the computerized color matching system across different skin colors. MATERIALS AND METHODS. The silicone was colored to simulate 28 different skin colors (n=5) to serve as "target skin colors". Using a spectrocolorometer (e-skin), color codes were determined for "replicate skin color" fabrication. CIELAB Delta-E between target skin color-replicate skin color pairs and translucency parameter were calculated. CIELAB Delta-E values were compared with one-way ANOVA and Tukey multiple-comparison. The agreement between $L^*$, $a^*$, $b^*$ and translucency parameter of target skin colors and replicate skin color were calculated by a two-way mixed average measures intraclass correlation coefficient. Translucency parameter of target skin color- replicate skin color pairs were compared with Paired t-test (${\alpha}=.05$). RESULTS. The mean CIELAB Delta-E value was 3.83 and significant differences were found among colors. The intraclass correlation coefficient showed excellent reliability for $L^*$, $a^*$, $b^*$ and good reliability for translucency parameter (P<.001). The mean translucency parameter of replicate skin colors was significantly higher than that of translucency parameter. CONCLUSION. The computerized color matching system specific to human skin was found to be reliable in terms of color and translucency between target skin colors and replicate skin color.

Porcine tissue factor promoter induces specific expression of target gene in response to human serum in porcine aortic endothelial cells

  • No, Jin Gu;Lee, Haesun;Lee, Na Hyun;Sun, Wu-Sheng;Hwang, In-Sul;Lee, Minguk;Oh, Keon Bong
    • Korean Journal of Agricultural Science
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    • v.48 no.2
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    • pp.353-365
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    • 2021
  • Previously, we reported that tissue factor (Tf) was included in the list of differentially expressed genes as an upregulated gene in a rejected porcine heart after xenotransplantation into monkey. In this study, we analyzed that expression of Tf in aortic endothelial cells (pAEC) isolated from alpha 1,3-galactosyltransferase knockout pig in response to allogeneic porcine serum and xenogeneic human serum. The consequence was significant upregulation of Tf expression by responding to human serum compared with porcine serum. To analyze the function of Tf gene as a promoter, we constructed reporter vectors for expression of luciferase linked to 1,246 and 787 base pairs of porcine Tf (pTF1246 and pTF787), and 535 base pairs of human TF (hTF535) sequences including putative promoter regions and AP-1 biding site at the 5' end. The reporter vectors were transfected into pAEC including cytomegalovirus enhancer/chicken β-actin (CAG)-luciferase vector as a control. Luciferase assay showed that all of the promoters were insufficient to express luciferase compared with CAG promoter in basic culture conditions. Notably, pTF1246, pTF787, and hTF535 led to a significant increase of luciferase expression in response to human serum compared with porcine serum while no change of CAG. pTF1246 and pTF787 showed higher expression than hTF535. Taken together, our findings suggest that pTF1246 and pTF787 promoters could mediate target gene expression specifically at xenogeneic stress condition.