• 생약학회지
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• 제2호2호
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• pp.95-98
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• 1971

In order to detect poisonous or physiologically active constituents in the Higher Fungi growing in Korea, forty three species including two varieties were collected in Kyung Ki and Chung Cheong Buk Do Provinces and subjected to systematic extraction with two kinds of organic solvents. The extracts were examined for alkaloid by adding alkaloid reagents. Of these fungi, three species, Geaster fimbriatus, Lampteromyces japonicus and Pseudocolus schellenbergiae, gave positive confirmatory tests.

• 한국균학회지
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• 제3권1호
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• pp.27-30
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• 1975

1957년(年)에 저자(著者)가 발표(發表)한 한국균심목록중 49종(種)이 아직 우라나라 이름이 었으므로 이를 신칭(新稱)하고자 1974년(年) 11월(月) 30일(日) 제(第)3회(回) 한국균학회학술발표회(韓國菌學會學術發表會)에 제안발표(提案發表)하여 명명(命名)하였다(Part I). 또 최근(最近)에 저자(著者)들에 의하여 연구(硏究)된 기록종(記錄種) 16종(種)도 명명(命名)하였다(Part II). 부록(附錄) I에는 홍순우(洪淳佑), 정학성(鄭學聲) 동(同) II에는 이응래(李應來), 정학성(鄭學聲)이 발표(發表) 명명(命名)한 보통명(普通名)을, 그리고 동(同) III에는 임정한(林鼎漢)의 기록(記錄) 5종(種)을 저자(著者)가 명명(命名)한 것을 첨가(添加)하였다.

• Journal of Ginseng Research
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• 제36권2호
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• pp.211-217
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• 2012

Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and flower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

• Mycobiology
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• 제31권2호
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• pp.86-94
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• 2003

The effect of four sub-lethal concentrations(400, 800, 1,200 and 1,600 ${\mu}g/ml$) of three amino acids such as isoluecine, aspartic acid and phenylalanine on vegetative growth and sexual and asexual reproduction of Achlya racemosa, A. proliferoides and Saprolegnia furcata was investigated. The density of vegetative growth and diameters of vegetative colonies of species of the Oomycetes fungi decreased with rising the concentration of the applied amino acid. Vegetative hyphae of treated fungi almost appeared branched in case of S. furcata, thick in case of A. racemosa and distorted in case of A. proliferoides as compared with control. The different treatments with amino acids depressed both sporangial formation and discharge, which were dependent on the tested species of zoosporic fungi, the amino acid and its dosage. Phenylalanine was the most effective amino acid in inhibiting sporulation and S. furcata was the most sensitive fungal species. Aspartic acid and isoleucine stimulated germination of discharged spores through the formation of germlings. Gemmae formation by the three fungi was reduced at the low concentrations of amino acids and nearly missed at high concentrations. Sex organs(oogonia and antheridia) were affected partly; rudiment oogonia were observed at low concentrations(400 and 800 ${\mu}g/ml$) and disappeared at higher concentrations, whereas antheridial branch formation was stimulated as the fungi were treated with isoleucine and to some extent phenylalanine.

• 한국환경복원기술학회지
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• 제3권1호
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• pp.70-79
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• 2000

Arbuscular mycorrhizal(AM) fungi have significant role for ecosystem structure and function. They are the major component of forest soil ecosystems and critically important for water and nutrient cycling in the system. To understand the ecology of AM fungi the fungal spores were collected, identified and counted in forest soils under various climatic and edaphic conditions. In relation to soil depth 90% of AM fungi spores and mycorrhizas distributed within 15cm soil depth. Number of spores per $100m{\ell}$ forest soil volume was 5 to 36 spores from 1 to 3 fungal species. AM fungal species diversity was higher in warmer climates, and more moist and fertile soils. The most frequently found species were Gigaspora decipiens irrespective of soil moisture and Gi. gigantea irrespective of soil fertility. In the Jeju island the soils of Cryptomeria japonica plantations and Miscanthus sinensis var. purpurascens meadow had more AM spores than the other soils. We suggest AM fungi be considered as keystones species when restoring a disturbed forest ecosystem.

• Mycobiology
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• 제49권4호
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• pp.396-405
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• 2021

Cinnamomum migao plants often face different degrees of drought in karst habitats, which can lead to plants' death, especially in the seedling stage. Widespread of arbuscular mycorrhizal (AM) fungi in karst soils have the potential to address this drought, which is a threat to C. migao seedlings. We inoculated C. migao seedlings with spores from Glomus lamellosum and Glomus etunicatum, two AM fungi widely distributed in karst soils, to observe seedling growth response after simulated drought. Our results showed that 40 g of G. lamellosum and G. etunicatum significantly promoted the growth of C. migao seedlings, 120 days after inoculation. Following a 15-day drought treatment, root colonization of the seedlings with G. lamellosum or G. etunicatum had lower the accumulation of malondialdehyde (MDA) and increased the accumulation of enzymes and osmotic substances in the seedlings. The relative water content in different organs (roots, stems, and leaves) of the drought-stressed seedlings was higher in plants with G. lamellosum or G. etunicatum than in plants without AM fungi colonization. Our results showed that inoculation with AM fungi was an effective means to improve the drought resistance of C. migao seedlings.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2180-2189
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• 2017

Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of the histidine acid phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified, and characterized. The phytase had an optimum temperature at $25^{\circ}C$, which is the lowest among all the known phytases to our best knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut. At the optimum pH and temperature, MiPhyA showed the maximum activity level ($2,384.6{\pm}90.4{\mu}mol{\cdot}min^{-1}{\cdot}mg^{-1}$, suggesting that the enzyme possesses a higher activity level over many known phytases at low temperatures. The phytate-degrading efficacy was tested on three common feed materials (soybean meal/rapeseed meal/corn meal) and was compared with the well-known phytases of Escherichia coli and A. niger. When using the same amount of activity units, MiPhyA could yield at least $3{\times}$ more inorganic phosphate than the two reference phytases. When using the same weight of protein, MiPhyA could yield at least $5{\times}$ more inorganic phosphate than the other two. Since it could degrade phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, MiPhyA might be a promising candidate as a feed additive enzyme.

• 한국균학회지
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• 제42권1호
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• pp.9-20
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• 2014

2009년 4월부터 2011년 10월까지 월출산국립공원 고등균류를 조사한 결과는 다음과 같다. 조사기간 동안 고등균류는 총 1계 2문 5강 18목 56과 133속 298종이었으며, 담자균문은 12목 47과 120속 278종이, 자낭균문은 6목 9과 13속 20종이 조사되었다. 대부분의 고등균류는 담자균문중 담자균강에 속하는 것으로 나타났다. 주름버섯목이 20과 54속 134종이, 그물버섯목은 7과 20속 43종, 무당버섯목 3과 5속 44종 및 구멍장이버섯목 5과 21속 30종으로 4목의 종수가 총 251종으로 전체 발생 종수의 84. 2%로 대부분을 차지한 것으로 조사되었다. 가장 많이 발생된 균류는 무당버섯과로 37종이었으며, 그물버섯과(33종), 광대버섯과(29종), 주름버섯과(21종) 및 구멍장이버섯과(20종) 순으로 나타났다. 월별 분포에서는 7월(46과 94속 196종)이 가장 많았고 8월(43과 92속 171종), 9월(38과 74속 120종)의 순이었다. 고도별에서는 100~199 m(54과 127속 267종)에서 가장 많았고 200~299 m(42과 85속 160종), 300~399 m(18과 31속 46종) 순으로 나타났다. 대부분의 고등균류는 평균온도 $26.0{\sim}27.9^{\circ}C$, 최고온도 $32.0{\sim}34.9^{\circ}C$, 최저온도 $24.0{\sim}25.9^{\circ}C$ 및 강수량은 100 mm 이상인 시기에 다양한 종들이 발생하는 것으로 나타났다.

• 한국환경보건학회지
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• 제33권4호
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• pp.250-254
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• 2007

The reduction efficiency of air cleaner on particulate contaminants such as dust, airborne bacteria and fungi distributed in the office was relatively higher in case of office with workers than office without workers. This result would be attributed to workers' activities and physical characteristics of particulate contaminants. The air cleaner decreased a concentration of airborne bacteria more than airborne fungi, which implicates that difference of dust adsorption between airborne bacteria and fungi would affect an operation efficiency of air cleaner.

• 펄프종이기술
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• 제36권3호
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• pp.9-14
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• 2004

The useful fungi which secret extracellular enzymes was selected for deinking agent of old newsprint. Five fungal strains were isolated from a paper mill soil ground. The CMCase, FPase and xylanase activities of fungi on the liquid culture were investigated at optimal growth conditions. The results of this study were as follow: The optimal pH and temperature for culture growth were 4～8 and 27～$35^{\circ}C$, respectively. For screening of extracellular enzymes at optimal culture conditions the optimal culture period were less than 6-7 days. Fusarium pallidoroseum and Aspergiilus niger which shows relatively higher CMCase, FPase and xylanase activities than the other species were selected for further enzymatic deinking research.