• Title/Summary/Keyword: high-temperature tolerance

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From food wastes into useful probiotics: Development of Lactic acid bacteria as useful probiotics for animal feed without antibiotics using food waste (음식물류폐기물의 자원화를 위한 항세균 활성 유산균의 개발)

  • Seo, Jeong-Yong;Song, In-Geun;Lee, Dae-Gyu;Lee, Ki-Young;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.2
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    • pp.112-120
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    • 2006
  • For the conversion of food waste into a good recycling material such as animal feed without antibiotics, thirteen lactic acid bacteria, which can be used as good probiotics for animal feed, were isolated from the intestine and feces in pigs. All isolates showed strong tolerance to high salt (4% of NaCl), acid(pH4.0), and bile juice (0.8% of oxgall). The growth rate was best at $37^{\circ}C$ in all strains. Among the isolates, Lactobacillus plantarum CJY-22, L. brevis CJY-42, L. arizonensis CJY-3, and Pediococcus sp. CJY-41 showed higher and broader spectrum of antimicrobial activities against six different pathogens such as Salmonella, typhimurium. L. plantarum CJY-22 has also grown well at $25^{\circ}C$, making this strain as an appropriate candidate for the fermentation of food waste at room temperature, thus conducting the fermentation process cost-effectively.

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Modeling the Present Probability of Urban Woody Plants in the face of Climate Change (기후변화에 따른 도시 수종의 기후 적합성 평가모델 - 서울시를 대상으로 -)

  • Kim, Yoon-Jung;Lee, Dong-Kun;Park, Chan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.16 no.1
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    • pp.159-170
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    • 2013
  • The effect of climate change on urban woody plants remains difficult to predict in urban areas. Depending on its tolerances, a plant species may stay and survive or stay with slowly declining remnant populations under a changing climate. To predict those vulnerabilities on urban woody plants, this study suggests a basic bioclimatic envelop model of heat requirements, cold tolerance, chilling requirements and moisture requirements that are well documented as the 'climatic niche'. Each component of the 'climatic niche' is measured by the warmth index, the absolute minimum temperature, the number of chilling weeks and the water balance. Regarding the utility of the developed model, the selected urban plant's present probabilities are suggested in the future climate of Seoul. Both Korea and Japan's thermal thresholds are considered for a plant's optimal climatic niche. By considering the thermal thresholds of these two regions for the same species, the different responses observed will reflect the plant's 'hardening' process in a rising climate. The model illustrated that the subpolar plants Taxus cuspidata and Ulmus davidiana var. japonica are predicted to have low suitability in Seoul. The temperate plants Zelkova serrata and Pinus densiflora, which have a broad climatic niche, exhibited the highest present probability in the future. The subtropical plants Camellia japonica and Castanopsis cuspidata var. sieboldii may exhibit a modest growth pattern in the late 21C's future climatic period when an appropriate frost management scheme is offered. The model can be used to hypothesize how urban ecosystems could change over time. Moreover, the developed model can be used to establish selection guidelines for urban plants with high levels of climatic adaptability.

Distribution of Alexandrium tamarense in Drake Passage and the Threat of Harmful Algal Blooms in the Antarctic Ocean

  • Ho, King-Chung;Kang, Sung-Ho,;Lam Ironside H.Y.;Ho, dgkiss I.John
    • Ocean and Polar Research
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    • v.25 no.4
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    • pp.625-631
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    • 2003
  • While phytoplankton diversity and productivity in the Southern Ocean has been widely studied in recent years, most attention has been given to elucidating environmental factors that affect the dynamics of micro-plankton (mainly diatoms) and nano-plankton (mainly Phaeocystis antarctica). Only limited effects have been given to studying the occurrence and the potential risks associated with the blooming of dinoflagellates in the relevant waters. This study focused on the appearance and toxicological characteristics of a toxic dinoflagellate, Alexandrium tamarense, identified and isolated from the Drake Passage in a research cruise from November to December 2001 The appearance of A. tamarense in the Southern Ocean indicates the risk of a paralytic shellfish poisoning (PSP) outbreak there and is therefore of scientific concern. Results showed that while the overall quantity of A. tamarense in water samples from 30meters below the sea surface often comprised less than 0.1% of the total population of phytoplankton, the highest concentration of A. tamarense (20 cells $L^{-1}$) was recorded in the portion of the Southern Ocean between the southern end of South America and the Falkland Islands. Waters near the Polar Front contained the second highest concentrations of 10-15 cells $L^{-1}$. A. tamarense was however rarely found in waters near the southern side of the Polar Front, indicating that cold sea temperatures near the Antarctic ice does not favor the growth of this dinoflagellate. One strain of A. tamarense from this cruise was isolated and cultured for further study in the laboratory. Experiments showed that this strain of A. tamarense has a high tolerance to temperature variations and could survive at temperatures ranging from $5-26^{\circ}C$. This shows the cosmopolitan nature off. tamarense. With regard to the algal toxins produced, this strain of A. tamarense produced mainly C-2 toxins but very little saxitoxin and gonyailtoxin. The toxicological property of this A. tamarense strain coincided with a massive death of penguins in the Falkland Islands in December 2002 to January 2003.

Low Power NDIR CO2 Sensor Using LED Light Source with a Smart Device Interface (스마트 기기와 결합 가능한 LED 광원을 사용하는 저전력용 비분산 적외선 CO2센서)

  • Kim, Jong-heon;Lee, Chan-joo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.8
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    • pp.1606-1612
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    • 2015
  • In this paper, a portable high efficiency nondispersive infrared(NDIR) $CO_2$ sensor module with a smart device interface is developed. For low power consumption design, an IR LED was used instead of tungsten lamp for light source and an optical waveguide optimized to the sensor module is designed. With the smart device interface, power of the sensor module is applied from the battery of smart phone. The measured data of the sensor module such as $CO_2$ concentration, temperature, and humidity are displayed on the smart phone using android application. From measured results, the developed sensor module shows ${\pm}60ppm$ tolerance error from 0 to 3,000ppm $CO_2$ concentration range among $-10^{\circ}C$ and $50^{\circ}C$ ranges.

The Operation of Polymer Electrolyte Membrane Fuel Cell using Hydrogen Produced from the Combined Methanol Reforming Process

  • Park, Sang Sun;Jeon, Yukwon;Park, Jong-Man;Kim, Hyeseon;Choi, Sung Won;Kim, Hasuck;Shul, Yong-Gun
    • Journal of Electrochemical Science and Technology
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    • v.7 no.2
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    • pp.146-152
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    • 2016
  • A combined system with PEMFC and reformer is introduced and optimized for the real use of this kind of system in the future. The hydrogen source to operate the PEMFC system is methanol, which needs two parts of methanol reforming reaction and preferential oxidation (PROX) for the hydrogen fuel process in the combined operation PEMFC system. With the optimized methanol steam reforming condition, we tested PROX reactions in various operation temperature from 170 to 270 ℃ to investigate CO concentration data in the reformed gases. Using these different CO concentration, PEMFC performances are achieved at the combined system. Pt/C and Ru promoted Pt/C were catalysts were used for the anode to compare the stability in CO contained gases. The alloy catalyst of PtRu/C shows higher performance and better resistance to CO than the Pt/C at even high CO amount of 200 ppm, indicating a promotion not only to the activity but also to the CO tolerance. Furthermore, in a system point of view, there is a fluctuation in the PEMFC operation due to the unstable fuel supply. Therefore, we also modified the methanol reforming by a scaled up reactor and pressurization to produce steady operation of PEMFC. The optimized system with the methanol reformer and PEMFC shows a stable performance for a long time, which is providing a valuable data for the PEMFC commercialization.

Isolation and Characterization of Protease Producing B. amyloliquefaciens JH-35 from Food Waste (음식물 쓰레기로부터 Protease를 생산하는 B. amyloliquefaciens JH-35의 분리 및 특성)

  • Yoo, Jae Hong;Joo, Jin Ho;Kim, Sung Gug;Jang, In-Hwan
    • Korean Journal of Environmental Agriculture
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    • v.35 no.4
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    • pp.294-301
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    • 2016
  • BACKGROUND: Recent studies have described the importance of microbes and enzymes that can compost food waste. This study was carried out to improve production of protease of isolated microbes from food waste. METHODS AND RESULTS: Seven bacteria isolated from various sources were screened for protease production by adding skim milk into the agar medium. About 7 microbes producing protease were tested, and strain JH-35 showed the highest protease activity among them. The strain was identified as Bacillus amyloliquefaciens JH-35 based on morphological, cultural, physiological characteristics and 16S rRNA. In the fermentation experiment, the assay B. amyloliquefaciens JH-35 showed the highest protease activity in the condition of 1% glucose, 1.5% yeast extract and 0.2%$ K_2HPO_4$. The optimal condition of culture with temperature $35^{\circ}C$, initial pH of 7 and shaking speed of 200 rpm and 24 hr. CONCLUSION: The protease of the B. amyloliquefaciens JH-35 had its activity at pH 7 and the optimal culture time was 24 hr. Also, B. amyloliquefaciens JH-35 was high salt tolerance. Our results suggest that B. amyloliquefaciens JH-35 from food waste may have the potential to degrade protein and carbohydrate in food waste.

Finite Element Analysis of Pilgering Process of Multi-Metallic Layer Composite Fuel Cladding (다중금속복합층 핵연료 피복관의 필거링 공정에 관한 유한 요소 해석 연구)

  • Kim, Taeyong;Lee, Jeonghyeon;Kim, Ji Hyun
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.13 no.2
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    • pp.75-83
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    • 2017
  • In severe accident conditions of light water reactors, the loss of coolant may cause problems in integrity of zirconium fuel cladding. Under the condition of the loss of coolant, the zirconium fuel cladding can be exposed to high temperature steam and reacted with them by producing of hydrogen, which is caused by the failure in oxidation resistance of zirconium cladding materials during the loss of coolant accident scenarios. In order to avoid these problems, we develop a multi-metallic layered composite (MMLC) fuel cladding which compromises between the neutronic advantages of zirconium-based alloys and the accident-tolerance of non-zirconium-based metallic materials. Cold pilgering process is a common tube manufacturing process, which is complex material forming operation in highly non-steady state, where the materials undergo a long series of deformation resulting in both diameter and thickness reduction. During the cold pilgering process, MMLC claddings need to reduce the outside diameter and wall thickness. However, multi-layers of the tube are expected to occur different deformation processes because each layer has different mechanical properties. To improve the utilization of the pilgering process, 3-dimensional computational analyses have been made using a finite element modeling technique. We also analyze the dimensional change, strain and stress distribution at MMLC tube by considering the behavior of rolls such as stroke rate and feed rate.

Changes in Non-Structural Carbohydrate in Overwintering Creeping Bentgrass (Agrostis palustris) (크리핑 벤트그라스의 월동중 비구조적 탄수화물의 변화)

  • Kim, Dae-Hyun;Jung, Woo-Jin;Lee, Bok-Rye;Kim, Kil-Yong;Kim, Tae-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.4
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    • pp.259-264
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    • 2001
  • To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. The concentration of glucose, fructose and sucrose in both shoot and root gradually increased from November to January, and then sharply decreased until April. Sucrose was the largest pool of soluble sugars. Fructan also slightly accumulated in both shoot and roots from November to February. Fructan hydrolysis in both organs was found to be much active between February to April. Shoot contained largely higher carbohydrate content in all compounds examined than roots did. Fructan was found to be a main carbohydrate storage form, showing the highest concentration (176.7 and 126.7 mg g-' DW for shoot and root in February). The depolymerization of fructan from February coincided with the high declines in mono- and disaccharide. These results suggest that the accumulation of non-structural carbohydrate until January could be associated with freezing tolerance, and the active decrease from February with shoot regrowth.

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Optimization of Thermal Deformation in Probe Card (프로브 카드의 열변형 최적화)

  • Chang, Yong-Hoon;Yin, Jeong-Je;Suh, Yong-S.
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.11
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    • pp.4121-4128
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    • 2010
  • A probe card is used in testing semiconductor wafers. It must maintain a precise location tolerance for a fine pitch due to highly densified chips. However, high heat transferred from its lower chuck causes thermal deformations of the probe card. Vertical deformation due to the heat will bring contact problems to the pins in the probe card, while horizontal deformation will cause positional inaccuracies. Therefore, probe cards must be designed with proper materials and structures so that the thermal deformations are within allowable tolerances. In this paper, heat transfer analyses under realistic loading conditions are simulated using ANSYS$^{TM}$ finite element analysis program. Thermal deformations are calculated based on steady-state temperature gradients, and an optimal structure of the probe card is proposed by adjusting a set of relevant design parameters so that the deformations are minimized.

Research to Minimize Endoscope and Objective-lens Sensitivity Using Multi-configurations (다중 구성을 이용한 내시경 및 대물렌즈 광학계 공차 민감도 최소화 설계 기술)

  • Jung, Mee-Suk
    • Korean Journal of Optics and Photonics
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    • v.32 no.6
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    • pp.259-265
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    • 2021
  • Recently, lens manufacturing and assembly technology has greatly improved. However, tight requirements of manufacturing and assembly lead to an increase in cost and manufacturing time, and in some cases the performance of an optical system may deteriorate depending on the operating environment's conditions, such as temperature or vibration. In addition, the use of a compensator is an effective method to reduce sensitivity in an ultra-precision optical system, but in the case of a small lens, such as that in an endoscope, it is difficult to use a compensator due to the size limitation of the lens barrel. Therefore, minimizing lens sensitivity is the most important technology in lens design. For this reason, there have been various attempts to reduce the lens sensitivity, and there is a trend to add functions to reduce the sensitivity in the lens design S/W. In this paper, we introduce a design technology that minimizes lens sensitivity. We first design a lens with quite good performance, then analyze the sensitivity of this lens, make a multi-configuration with high-sensitivity element error, and then reoptimize it. We prove with an example that this design technique is very effective.