• 제목/요약/키워드: high-pressure extract method

검색결과 51건 처리시간 0.035초

퍼지 알고리듬을 이용한 오프라인 서명 검증에 관한 연구 (A Study on Off Line Signature Verification using by Fuzzy Algorithm)

  • 이상범;박남수;최한석;이계영
    • 전자공학회논문지B
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    • 제31B권7호
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    • pp.1-8
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    • 1994
  • There are many research activities in various recognition areas using high calibered computing power. Among many areas, the signature recognition and verification have more difficulties than any other recognition area because signature itself contains many problems caused by a variation of psychological status of signer and other environment. In the case of signature, therefore, it is important to extract the better parameters required for the higher verification ratio. In this paper, signature pressure is extracted and used as feature parameters to determine whether the input signature is ture or forgery, and then input signature is verified by fuzzy similarity method. As a result of appling the fuzzy similarity method to the recognition system it is proven that the system has by far better verification ratio about 10% than existing methods.

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초고압을 이용한 반고형 사과 이유식 개발 및 품질평가 (Development of Semi-Solid Apple Baby Food using High Pressure Processing and Quality Evaluation)

  • 조형용;조은경;김병철;신해헌
    • 한국식품영양학회지
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    • 제24권4호
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    • pp.777-785
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    • 2011
  • 비열처리가공기술 중 하나로 새롭게 주목받고 있는 초고압처리를 반고형 형태의 친환경 유기농 이유식 제조에 적용하여 완제품 형태로 제조 후 냉장 조건으로 저장하여 저장기간에 따른 제품 안전성을 조사하였다. 가열 처리를 하지 않고 550 MPa 압력에서 3분 동안 초고압 처리하여 냉장 상태로 15일 동안 저장한 결과, 일반세균과 대장균 모두 검출되지 않아 미생물학적 안전성을 확인할 수 있었으며, 초고압 처리를 하지 않은 대조군에서 일반세균이 $2.54{\times}10^3$, $7.85{\times}10^2$ CFU/g 수준으로 검출되었던 것과 비교했을 때 초고압 처리를 하는 것이 제품의 신선도를 살리고 제품의 안전성을 유지하기 위해 적합한 처리법이라고 판단되어진다. 초고압 처리를 통한 물성 변화는 처리 전 대조구와 점도, 스푼풀 현상을 비교하였을 때 큰 차이가 나타나지 않는 것으로 나타났고, 그 결과 사과의 신선한 상태를 유지하고 미생물학적 안전성 역시 유지하면서 냉장상태로 약 2주간 저장이 가능한 친환경 반고형 이유식을 제조할 수 있었다.

자외선이 조사된 인간피부섬유아세포에 복분자 추출물이 미치는 영향 (Effects of Rubus coreanus Extracts on Ultraviolet-A Irradiated Cultured Human Skin Fibroblasts)

  • 정향숙;하지혜;김영;오성호;김승섭;정명훈;이현용
    • 한국약용작물학회지
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    • 제17권5호
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    • pp.321-327
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    • 2009
  • Sunlight, and in particular its UV component, is the major environmental trigger that underlies the major signs of human skin and skin cancer in general. Therefore, this study was carried out to investigate the UV protection effects of R. coreanus. R. coreanus was extracted by ultra high pressure extraction process at 500 MPa and $30^{\circ}C$ for 5 and 15 minutes. The cytotoxicity of the extracts extracted by ultra high pressure process on human dermal fibroblast cell CCD-986sk, human kidney normal cell HEK293, and human lung normal cell HEL299 was measured as 17.5%, 16.5% and 14.0%, respectively in adding $1.0\;mg/m{\ell}$ of the samples, which was much lower than that from conventional water extraction method at $100^{\circ}C$ as 23.2%, 22.5%, 21.2%. The secretion of $NO^-$ from macrophage showed $15.9\;{\mu}M$ on the R. coreanus extract from this process, which was higher than others. Prostaglandin $E_2$ ($PGE_2$) production from UV-induced human skin cells was also greatly decreased down to $510\;pg/m{\ell}$, compared to the control. From the results, we considered that the extracts from R. coreanus could be potent natural materials for skin anti-inflammation agent, and could be used as a potential anti-aging for the photo-damaged skin.

추출법에 따른 당귀수산과 작약감초탕의 성분과 활성의 비교 (Comparison of Ingredients and Activities of Danggwisoo-san and Jakyakgamcho-tang by Extraction Method)

  • 이대연;이호성;조주휘;이영우;김성진;강경래;권태욱;양승구;이인희
    • 한방재활의학과학회지
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    • 제30권4호
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    • pp.31-39
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    • 2020
  • Objectives Danggwisoo-san and Jakyakgamcho-tang are frequently prescribed for traffic accident patients in Korea. The aim of this study was to examine index compound analysis, antioxidant activity and amount of starch measurement by extraction method. Methods Danggwisoo-san and Jakyakgamcho-tang were extracted with water and 70% ethanol. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power according to the standard protocol. The contents of the indicator components nodakenin, paeoniflorin, and glycyrrhizin were measured by high-performance liquid chromatography, respectively. All starches were hydrolyzed and then total D-glucose was measured and compared. Results Antioxidant activity was excellent in 70% ethanol in all assays. The index component was jagged because its solubility was different depending on the extraction solvent. Starch content was significantly lower in 70% alcohol extract than water extract. Conclusions The results of this study showed that physiological activities and components are different according to extraction conditions. Each herbal medicine has a suitable extraction solvent. Also, the difference in starch content is an object to be considered as it may affect digestion and absorption.

Liquid Chromatographic Determination of Etofenprox Residues in Foods with Mass-Spectrometric Confirmation

  • Lee, Young-Deuk;Kwon, Chan-Hyeok;Kwon, Ki-Sung
    • 한국환경농학회지
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    • 제30권4호
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    • pp.432-439
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    • 2011
  • BACKGROUND: An official analytical method was developed to determine etofenprox residues in agricultural commodities using high-performance liquid chromatography (HPLC). METHODS AND RESULTS: The etofenprox residue was extracted with acetone from representative samples of five raw products which comprised rice grain, apple, mandarin, cabbage, and soybean. The extract was then serially purified by liquid-liquid partition and Florisil column chromatography. For rice and soybean samples, acetonitrile/n-hexane partition was additionally coupled to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate etofenprox from co-extractives. Intact etofenprox was sensitively detected by ultraviolet absorption at 225 nm. Recovery experiment at the quantitation limit validated that the proposed method could apparently determine the etofenprox residue at 0.02 mg/kg. Mean recoveries from five crop samples fortified at three levels in triplicate were in the range of 93.6~106.4%. Relative standard deviations of the analytical method were all less than 10%, irrespective of crop types. A selected-ion monitoring LC/mass spectrometry with positive atmospheric-pressure chemical ionization was also provided to confirm the suspected residue. CONCLUSION(s): The proposed method is simple, rapid and sensitive enough to be employed in routine inspection or monitoring of agricultural products for the etofenprox residue.

고로쇠 수피 초고압 추출물의 항암활성 증진 (Enhancement of Anticancer Activity of Acer mono by High Pressure Extraction Process)

  • 정명훈;김승섭;하지혜;김영;이학주;강하영;박성진;이현용
    • 한국식품영양과학회지
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    • 제38권9호
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    • pp.1243-1252
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    • 2009
  • 본 연구에서는 초고압 공정을 통하여 기존의 일반 열수추출공정과 비교함으로써 초고압 공정에 의한 항산화 활성 증진 및 항암활성 증진 효과를 확인하였다. 기존의 추출공정과 비교하여 높은 압력 하에서 추출수율이 증가한 것은 시료의 세포막이 비가역적으로 분해되어 막 투과성이 증가되어 물질 이동이 용이하게 되어 용매가 세포 안으로 들어가 보다 많은 성분이 세포 밖으로 쉽게 용출되어 나오는 결과라고 추정된다. DPPH radical 소거활성을 측정한 결과, 네 가지의 추출물 중 15분간 초고압 처리한 추출물의 활성이 1.0 mg/mL의 농도에서 94.56%로 가장 높은 활성을 보였고, 또한 BHA의 93.24%보다 높은 활성을 보였다. SOD 유사활성 결과를 살펴보면 15분간 초고압 처리를 한 추출물의 활성이 모든 농도에서 대조군으로 비교한 BHA의 활성보다 높았고, 열수추출의 활성보다 높은 결과를 나타내었다. 인간의 정상 폐 세포인 HEL299를 이용한 세포 독성은 초음파 공정을 15분간 처리한 추출물 1.0 mg/mL의 농도에서 20.12%로, 일반 열수 추출물의 24.53%보다 낮은 세포독성을 나타내어 초고압 공정을 통한 세포독성 저감 효과를 확인할 수 있었다. 또한 인간 정상 신장 세포인 HEK293을 이용한 세포 독성실험에서도 1.0 mg/mL의 농도에서 초고압 처리를 15분 동안 한 추출물이 23.54%를 나타내어 일반 열수 추출물보다 약 3% 낮은 독성을 보였다. 암세포 억제활성 측정은 AGS, A549, MCF-7, Hep3B, Caco-2의 세포를 이용하여 실험을 하였다. 위의 4가지 암세포에서 1.0 mg/mL의 농도에서 70% 이상의 높은 암세포 억제 활성을 보였고, 암세포의 생육활성에 대한 정상 세포의 세포독성의 비로 나타낸 선택적사멸도는 1.0 mg/mL의 농도에서 4 정도로 나타났으며, 초고압 공정에 의한 추출물의 사멸도가 높게 나타났다. 본 실험 결과를 통하여 기존의 일반 열수 추출에 비해 초고압 공정을 통한 추출물의 수율향상 및 세포독성 저감 효과를 확인할 수 있었는데, 이는 초고압에 의한 고로쇠 수피 표면조직의 파괴와 성분 변화로 인한 유용물질 및 신물질의 대량 용출과 함께 독성물질이 파괴되거나 변성되어 나타난 결과라고 생각 된다. 또한 항산화 활성 및 항암활성 증진효과를 확인할 수 있었는데 이를 통해 고로쇠나무의 항산화제 및 항암 신소재로의 가능성을 확인할 수 있었다. 더 나아가 초고압 공정에 의해 용출된 성분의 분리 및 성분 분석에 관한 연구가 더 필요할 것으로 사료된다.

Reaction Flavoring에 의한 진주조개 (Pinctada fucata) 추출물의 풍미개선 (Enhancing the Flavor of Pearl Oyster (Pinctada fucata) Extract Using Reaction Flavoring)

  • 강정구;남기호;강진영;황석민;김정균;오광수
    • 한국수산과학회지
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    • 제40권6호
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    • pp.350-355
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    • 2007
  • The optimal substrates and reaction flavoring conditions were examined to develop pearl oyster extract (POE) flavor using the Maillard reaction under a model system. The sugar for the Maillard reaction was glucose, and the amino acid was cysteine, with glycine as the reaction substrate. A three-dimensional response surface method was used to monitor the dynamic changes of the substrates during the Maillard reaction. To enhance the flavor of POE, a two-step enzymatic hydrolysate (Brix $20^{\circ}$) was reacted with the precursors (1:1, v/v). A 2:1:1 mixture of 0.4 M glucose:0.4 M glycine:0.4 M cysteine (v/v) was selected as a suitable reaction system for the reappearance of baked potato odor and boiled meat odor, and masking the shellfish odor. The two-step enzymatic hydrolysate and selected precursors were reacted in a high-pressure reactor to optimize the reaction parameters. The optimum conditions were 150 minutes at $120\;^{\circ}C$ and pH 7.0. The pH was the most critical factor for the response of the baked potato odor and masking the shellfish odor, while the reaction time affected the reappearance of the boiled meat odor.

고속 푸리에 합성곱을 이용한 파지 조건에 강인한 촉각센서 기반 물체 인식 방법 (Tactile Sensor-based Object Recognition Method Robust to Gripping Conditions Using Fast Fourier Convolution Algorithm)

  • 허현석;김정중;고두열;김창현;이승철
    • 로봇학회논문지
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    • 제17권3호
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    • pp.365-372
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    • 2022
  • The accurate object recognition is important for the precise and accurate manipulation. To enhance the recognition performance, we can use various types of sensors. In general, acquired data from sensors have a high sampling rate. So, in the past, the RNN-based model is commonly used to handle and analyze the time-series sensor data. However, the RNN-based model has limitations of excessive parameters. CNN-based model also can be used to analyze time-series input data. However, CNN-based model also has limitations of the small receptive field in early layers. For this reason, when we use a CNN-based model, model architecture should be deeper and heavier to extract useful global features. Thus, traditional methods like RN N -based and CN N -based model needs huge amount of learning parameters. Recently studied result shows that Fast Fourier Convolution (FFC) can overcome the limitations of traditional methods. This operator can extract global features from the first hidden layer, so it can be effectively used for feature extracting of sensor data that have a high sampling rate. In this paper, we propose the algorithm to recognize objects using tactile sensor data and the FFC model. The data was acquired from 11 types of objects to verify our posed model. We collected pressure, current, position data when the gripper grasps the objects by random force. As a result, the accuracy is enhanced from 84.66% to 91.43% when we use the proposed FFC-based model instead of the traditional model.

가죽나무(Ailanthus altissima) 열수 추출물의 생리활성 (Physiological Activities of Hot Water Extract from Ailanthus altissima)

  • 이양숙
    • 한국식품저장유통학회지
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    • 제14권2호
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    • pp.170-176
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    • 2007
  • 한방생약자원으로 사용되는 가죽나무(A. altissima)의 뿌리와 줄기, 잎의 열수 추출물을 농도에 따라 전자공여능, SOD유사활성, 아질산염 소거능을 측정하여 항산화 활성에 대하여 분석하였다. 그리고 통풍을 일으키는 원인으로 알려진 xanthine oxidase와 melanin 색소 합성에 작용하는 tyrosinase저해 활성을 측정하여 가죽나무의 생리활성을 분석하였다. 전자공여능은 1.0 mg/mL의 농도에서 뿌리(91.25%) > 줄기(67.03%) > 잎(47.94%)의 순 이었으며, SOD 유사활성능은 잎(49.07%) > 뿌리(14.31%) > 줄기(10.78%)의 순으로 나타났다. 아질산염 소거능은 pH 1.2의 조건하에서 잎에서는 93.33%, 줄기와 뿌리는 54.07%, 45.97%로 나타났으며, pH 3.0에서도 잎 추출물은 85.40%로 pH 1.2의 줄기와 뿌리 보다 높은 소거효과를 나타내었다. Xanthine oxidase 저해는 2.0mg/mL 농도에서 뿌리와 줄기가 97.44%와 97.41%, 잎에서도 92.09%였으며 1.0 mg/mL의 농도에서도 87% 이상의 높은 저해효과가 나타났다. Tyrosinase저해는 0.1mg/mL의 저농도에서 뿌리 추출물이 63.97%로 줄기(4.27%)와 잎(9.09%)보다 7배 이상 높았으며 2.0 mg/mL의 고농도에서도 뿌리는 67.38%로 줄기와 잎보다 높았으며, 농도의 증가에 따른 저해율은 큰 차이가 없었다. 이상의 결과 가죽나무는 한방생약자원으로 이용되는 뿌리와 줄기뿐만 아니라 잎도 우수한 생리활성을 나타내는 것으로 생각되며 뿌리 추출물은 소량으로도 높은 tyrosinase 저해효과를 나타내는 것으로 분석되었다.

WC-Co계 분말사출성형에서 초임계$CO_2$에 의한 결합제 제거 (Binder Removal by Supercritical $CO_2$ in Powder Injection Molded WC-Co)

  • 김용호;임종성;이윤우;김소나;박종구
    • 한국분말재료학회지
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    • 제8권2호
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    • pp.91-97
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    • 2001
  • The conventional debinding process in metal injection molding is very long time-consuming and unfriendly environmental method. Especially, in such a case of injection molded parts from hard and fine metal powder, such as WC-Co, an extremely long period of time is necessary in the conventional slow binder removal process. On the other hand, supercritical debinding is thought to be the effective method which is appropriate to eliminate the aforementioned inconvenience in the prior art. The supercritical fluid has high diffusivity and density, it can penetrate quickly into the inside of the green metal bodies, and extract the binder. In this paper, super-critical debinding is compared with wicking debinding process. Wax-based binder system is used in this study. The binder removal rate in supercritical $CO_2$ have been measured at $65^{\circ}C$, 75$^{\circ}C$ in the pressure range from 20 MPa to 28 MPa. Pores and cracks in silver bodies after sintering were observed using SEM When the super-critical $CO_2$ debinding was carried out at 75$^{\circ}C$, almost all the wax (about 70 wt% of binder) was removed in 2 hours under 28 MPa and 2.5 hours under 25 MPa.

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