• 생명과학회지
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• 제18권9호
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• pp.1312-1315
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• 2008

DAPI 염색을 이용하여 세포사멸 유도물질의 초고속스크리닝에 적합한 protocol을 제작하였다. Liquid handler에서 dispensing과 multilabel counter에서의 상대적인 fluorescent intensity를 측정하는 과정을 자동화한 protocol을 제작하였고, 이의 과정은 매우 효율적으로 가동되었음을 확인하였다. DAPI에 binding된 DNA의 intensity를 측정하는 조건검토에서 가장자리의 36개를 reading하지 않는 것이 edge effect를 줄일수 있는 것을 확인하였고, 0.1 sec reading으로 시간을 절약할 수 있었다. 이의 결과를 효과적으로 이용할 경우, 세포사멸 유도물질의 초고속스크리닝 또는 이에 적합한 스크리닝이 가능함을 확인하였다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제14권4호
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• pp.419-426
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• 2014

This paper proposes a high-throughput encoding process and encoder architecture for quasi-cyclic low-density parity-check codes in IEEE 802.11ac standard. In order to achieve the high throughput with low complexity, a partially parallel processing based encoding process and encoder architecture are proposed. Forward and backward accumulations are performed in one clock cycle to increase the encoding throughput. A low complexity cyclic shifter is also proposed to minimize the hardware overhead of combinational logic in the encoder architecture. In IEEE 802.11ac systems, the proposed encoder is rate compatible to support various code rates and codeword block lengths. The proposed encoder is implemented with 130-nm CMOS technology. For (1944, 1620) irregular code, 7.7 Gbps throughput is achieved at 100 MHz clock frequency. The gate count of the proposed encoder core is about 96 K.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• 대한방사성의약품학회지
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• 제8권2호
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• pp.95-101
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• 2022

Microfluidic radioimmunoassay (RIA) platform called µ-RIA spends less reagent and shorter reaction time for the analysis compared to the conventional tube-based radioimmunoassay. This study reported the design of µ-RIA chips optimized for the gamma counter which could measure the small samples of radioactive materials automatically. Compared with the previous study, the µ-RIA chips developed in this study were designed to be compatible with conventional RIA test tubes. And, the automatic gamma counter could detect radioactivity from the ¹²⁵I labeled anti-PSA attached to the chips. Effects of the multi-layer microchannels and two-phase flow in the µ-RIA chips were investigated in this study. The measured radioactivity from the ¹²⁵I labeled anti-PSA was linearly proportional to the number of stacked chips, representing that the radioactivity in µ-RIA platform could be amplified by designing the chips with multi-layers. In addition, we designed µ-RIA chip to generate liquid-gas plug flow inside the microfluidic channel. The plug flow can promote binding of the biomolecules onto the microfluidic channel surface with recirculation in the liquid phase. The ratio of liquid slug and air slug length was 1 : 1 when the ¹²⁵I labeled anti-PSA and the air were injected at 1 and 35 µL/min, respectively, exhibiting 1.6 times higher biomolecule attachment compared to the microfluidic chip without the air injection. This experimental result indicated that the biomolecular reaction was improved by generating liquid-gas slugs inside the microfluidic channel. In this study, we presented a novel µ-RIA chips that is compatible with the conventional gamma counter with automated sampler. Therefore, high-throughput radioimmunoassay can be carried out by the automatic measurement of radioactivity with reduced radiowaste generation. We expect the µ-RIA platform can successfully replace conventional tube-based radioimmunoassay in the future.

• 한국식품저장유통학회지
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• 제12권5호
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• pp.482-488
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• 2005

산화적인 스트레스(Oxidative stress)는 신경염증의 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충추출물을 이용하여 항산화 물질을 찾고자 초고속 적용가능한 스크리닝 방법을 적용하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 이들의 억제를 동반하는 추출물을 먼저 선별하였다. 항산화 실험과 관련하여 DPPH (1,1-Diphenyl-2-picrylhydrazyl), FRAP(Ferric ion reducing antioxidant power), HO (Hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 assay하였고 hydrogen peroxide(H2O2)에 의한 세포사멸 억제 활성을 보기 위해 MTT assay를 실시하였다. 실험 결과, 사마귀, 늦반딧불이, 무당벌레에서 다른 library에 비교하여 항산화 활성이 높게 나타났다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2005년도 추계종합학술대회
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• pp.837-840
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• 2005

This paper presents 3D graphics lighting processor based on vector processing using pipeline chaining. The lighting process of 3D graphics rendering contains many arithmetic operations and its complexity is very high. For high throughput, proposed processor uses pipelined functional units. To implement fully pipelined architecture, we have to use many functional units. Hence, the number of functional units is restricted. However, with the restricted number of pipelined functional units, the utilization of the units is reduced and a resource reservation problem is caused. To resolve these problems, the proposed architecture uses vector processing using pipeline chaining. Due to its pipeline chaining based architecture, it can perform 4.09M vertices per 1 second with 100MHz frequency. The proposed 3D graphics lighting processor is compatible with OpenGL ES API and the design is implemented and verified on FPGA.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 ICEIC The International Conference on Electronics Informations and Communications
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• pp.176-180
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• 2004

In this paper we introduce a bandwidth allocation algorithm and admission control policy for IEEE 802.16 broadband wireless access standard. The proposed mechanism is practical and compatible to the IEEE 802.16. Our scheme provides QoS support to high priority traffic and high throughput in low priority traffic. The simulation show that the proposed scheme includes QoS support for real-time traffic and we presented that BS determine a efficient contention mini-slot size. We have shown the relationship between traffic size and its QoS requirements and the network performance.

• Journal of Communications and Networks
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• 제15권3호
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• pp.292-300
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• 2013

Nowadays, energy saving has become a hot topic and information and communication technology has become a major power consumer. In long term evolution advanced (LTE-A) networks, heterogeneous deployments of low-power nodes and conventional macrocells provide some new features, such as coverage extension, throughput enhancement, and load balancing. However, a large-scale deployment of low-power nodes brings substantial energy consumption and interference problems. In this paper, we propose a novel switching strategy (NS), which adaptively switches on or off some low-power nodes based on the instantaneous load of the system. It is compatible with the microcells' load balancing feature and can be easily implemented on the basis of existing LTE-A specifications. Moreover, we develop an analytical model for analyzing the performance of system energy consumption, block rate, throughput, and energy efficiency. The performance of NS is evaluated by comparison with existing strategies. Theoretical analysis and simulation results show that NS not only has a low block rate, but also achieves a high energy efficiency.

• Journal of Communications and Networks
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• 제15권3호
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• pp.312-320
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• 2013

Home evolved Node-B (HeNB), also called a femtocell or a femto base station, is introduced to provide high data rate to indoor users. However, two main problems arise in femtocell networks: (1) Small coverage area of HeNB, which results in limited cell-splitting gain and ping-pong handover (HO) problems and (2) high inter-femtocell interference because HeNBs may be densely deployed in a small region. In this study, an efficient cooperation mechanism called an HeNB-aided virtual-HO (HaVHO) scheme is proposed to expand the coverage area of femtocells and to reduce inter-femtocell interference. The cooperation among neighbor HeNBs is exploited in HaVHO by enabling an HeNB to relay the data of its neighbor HeNB without an HO. The HaVHO procedure is compatible with the existing long term evolution specification, and the information exchange overhead in HaVHO is relatively low. To estimate the signal to interference plus noise ratio improvement, the area average channel state metric is proposed, and the amount of user throughput enhancement by HaVHO is derived. System-level simulation shows that HaVHO has a better performance than the other four schemes, such as lesser radio link failure, lesser ping-pong handover, lesser short-stay handover, and higher user throughput.

• The Plant Pathology Journal
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• 제27권4호
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• pp.349-353
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• 2011

The ascomycete fungus Fusarium graminearum is an important plant pathogen responsible for Fusarium head blight in small grains and ear rot on maize. This fungus also produces the estrogenic metabolite, zearalenone (ZEA) that causes estrogenic disorders in humans and animals. Previously, we developed a conditional gene expression system for this fungus using a ZEA-inducible promoter (Pzear). In the present study, four other estrogenic compounds, including ${\beta}$-estradiol, estriol, estrone, and secoisolariciresinol, were screened as possible substitutes for ZEA in this system. Among them, ${\beta}$-estradiol was able to successfully induce the expression of a gene controlled by Pzear, while estrone was only able to partially induce its expression but the other two compounds were not effective. In combination, these results demonstrate that ${\beta}$-estradiol can replace ZEA in this conditional gene expression system, thereby eliminating the need to use the more expensive reagent, ZEA, and facilitating high-throughput functional analyses of F. graminearum in future studies.