• Title/Summary/Keyword: high through-put analysis

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Antioxidant Activity of Solvent Fraction from Broccoli Sprouts Cultivated at the Plant Factory System (식물공장에서 재배한 브로콜리 새싹 용매 분획물의 항산화 활성)

  • Kim, Eun-Ji;Kim, Mi-Hye
    • The Korean Journal of Food And Nutrition
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    • v.28 no.1
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    • pp.1-8
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    • 2015
  • This study was designed to determine the antioxidant activity of solvent fractions of broccoli sprouts grown by controlling the growing environment at the plant factory system. Fractionation was achieved with chloroform, n-hexane, ethyl acetate, butanol, water by 70% EtOH extract of the broccoli sprouts. Each solvent fraction was put through TLC and HPLC to separate active components. Higher antioxidant activities were observed for the butanol and ethyl acetate layers. Further evaluation of each of the 5 layers (LH1 to LH5) of the butanol fraction showed that the refined LH3 extract had a high antioxidant effect. Components with similar Rf values from TLC had the same retention times and peaks in the HPLC analysis. It was also determined that the sulforaphane content was high at the chloroform and butanol layers and the sulforaphane was responsible for, the high antioxidant activity. Thus, to use for functional materials, the butanol extract/layer of broccoli sprouts is recommended as the most effective.

Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis (MALDI-MS 기반 당단백질 당쇄의 정량분석 기술 개발 연구 동향)

  • Kim, Kyoung-Jin;Kim, Yoon-Woo;Hwang, Cheol-Hwan;Park, Han-Kyu;Jeong, Jae Hyun;Kim, Yun-Gon
    • KSBB Journal
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    • v.30 no.5
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    • pp.230-238
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    • 2015
  • Abnormal glycosylation can significantly affect the intrinsic functions (i.e., stability and solubility) of proteins and the extrinsic protein interactions with other biomolecules. For example, recombinant glycoprotein therapeutics needs proper glycosylation for optimal drug efficacy. Therefore, there has been a strong demand for rapid, sensitive and high-through-put glycomics tools for real-time monitoring and fast validation of the biotherapeutics glycosylation. Although liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the most powerful tools for the characterization of glycan structures, it is generally time consuming and requires highly skilled personnel to collect the data and analyze the results. Recently, as an alternative method, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS), which is a fast, robust and easy-to-use instrumentation, has been used for quantitative glycomics with various chemical derivatization techniques. In this review, we highlight the recent advances in MALDI-MS based quantitative glycan analysis according to the chemical derivatization strategies. Moreover, we address the application of MALDI-MS for high-throughput glycan analysis in many fields of clinical and biochemical engineering.

Study on Influence and Diffusion of Word-of-Mouth in Online Fashion Community Network (온라인 패션커뮤니티 네트워크에서의 구전 영향력과 확산력에 관한 연구)

  • Song, Kieun;Lee, Duk Hee
    • Journal of the Korean Society of Costume
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    • v.65 no.6
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    • pp.25-35
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    • 2015
  • The purpose of this study is to investigate the characteristics of members and communities that have significant influence in the online fashion community through their word-of-mouth activities. In order to identify the influence and the diffusion of word-of-mouth in fashion community, the study selected one online fashion community. Then, the study sorted the online posts and comments made on fashion information and put them into the matrix form to perform social network analysis. The result of the analysis is as follows: First, the fashion community network used in the study has many active members that relay information very quickly. Average time for information diffusion is very short, taking only one or two days in most cases. Second, the influence of word-of-mouth is led by key information produced from only a few members. The number of influential members account for less than 20% of the total number of community members, which indicate high level of degree centrality. The diffusion of word-of-mouth is led by even fewer members, which represent high level of betweenness centrality, compared to the case of degree centrality. Third, component characteristic shares similar information with about 70% of all members being linked to maximize information influence and diffusion. Fourth, a node with high degree centrality and betweenness centrality shares similar interests, presenting strain effect to particular information. Specially, members with high betweenness centrality show similar interests with members of high degree centrality. The members with high betweenness centrality also help expansion of related information by actively commenting on posts. The result of this research emphasizes the necessity of creation and management of network to efficiently convey fashion information by identifying key members with high level of information influence and diffusion to enhance the outcome of online word-of-mouth.

Design development of athleisure bike wear to be a casual daily wear (일상복 겸용의 애슬레저 바이크 웨어 디자인 개발 연구)

  • Lee, Dal A;Ahn, In-Sook
    • The Research Journal of the Costume Culture
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    • v.24 no.6
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    • pp.788-802
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    • 2016
  • Sportswear transforms itself into a new fashion item to satisfy desires for beauty in addition to having functionality and movability for sports activities. Sportswear is not in fashion just temporarily but comes in various forms along with sociocultural phenomenon that put emphasis on well-being and leisure. This research aims to study the needs of the bikers who ride bicycles or cycles has become a popular outdoor activity, and give suggestions for high functional sports design that can satisfy the needs of the highly demanding taste of athletes. To understand the design development direction and design preference, practical consumer needs were derived through a survey analysis on bike wear purchasing satisfaction and actual wearing state by bike riders. For consumer needs analysis, focus group interview, in-depth interview and questionnaire method were used. Based on analysis of those researches, athleisure bike wears appropriate for urban sports activities were categorized into sports, life, fitness and leisure 4 styles for athleisure bike wear design development. It is believed that this study will contribute to in the category expansion of sports wear market and satisfy the consumer demands for functional and specialized casual bike wears as well as fashion factors optimized to life style of modern people.

A Study on Reinforced Concrete Beams with Perforation (철근콘크리트 유공보에 관한 연구)

  • Park, Kyong-Ho
    • Journal of Industrial Technology
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    • v.21 no.A
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    • pp.7-14
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    • 2001
  • In building structure, the story height can be minimized by providing openings in beams which serves for the utility equipments passing through. The dead space in false ceiling thus put to economical use in the form of a substantial reduction in materials and construction cost. In the case of steel structure, there is no critical risk in the structural strength because of reinforcing methods of stiffness and steel plate but in the case of reinforced concrete structure, proper provision should be made in designing these openings, otherwise there is a risk that these opening will possibly weaken the structural strength of the building frame to a critical degree. In this paper, for the numerical analysis of the reinforced concrete beams with circular opening in the web, expecting stress concentration of the circular opening, reinforcing methods were studied. Twenty test pieces with each different reinforcing methods were tested and their resisting forces were defined. From the numerical analysis and test results, the followings were founded;(1)high shear stress distributed around the openings reduce the shearing strength, (2)from the numerical analysis, the maximum tensile stress occurred at opening nodes 1,7, these phenomena were agreed with the test results, (3)reinforcing method around openings have to carried out for stopping diagonal cracks, and (4)both, by steel plate, and wire mesh, are effective reinforcing methods.

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Peak ground acceleration attenuation relationship for Mazandaran province using GEP algorithm

  • Ahangari, Hamed Taleshi;Jahani, Ehsan;Kashir, Zahra
    • Earthquakes and Structures
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    • v.15 no.4
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    • pp.403-410
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    • 2018
  • The choice of attenuation relationships is one of the most important parts of seismic hazard analysis as using a different attenuation relationship will cause significant differences in the final result, particularly in near distances. This problem is responsible for huge sensibilities of attenuation relationships which are used in seismic hazard analysis. For achieving this goal, attenuation relationships require a good compatibility with the target region. Many researchers have put substantial efforts in their studies of strong ground motion predictions, and each of them had an influence on the progress of attenuation relationships. In this study, two attenuation relationships are presented using seismic data of Mazandaran province in the north of Iran by Genetic Expression Programming (GEP) algorithm. Two site classifications of soil and rock were considered regarding the shear wave velocity of top 30 meters of site. The quantity of primary data was 93 records; 63 of them were recorded on rock and 30 of them recorded on soil. Due to the shortage of records, a regression technique had been used for increasing them. Through using this technique, 693 data had been created; 178 data for soil and 515 data for rock conditions. The Results of this study show the observed PGA values in the region have high correlation coefficients with the predicted values and can be used in seismic hazard analysis studies in the region.

Study of Flow Characteristics behind a Sunroof Wind Deflector for Wind Noise Reduction (바람소리 저감을 위한 선루프 디플렉터 주위의 유동에 관한 연구)

  • Lee, Dug-Young;Yoon, Jong-Hwan;Shin, Jae-Hyuk;Kim, Sang-Kon
    • Transactions of the Korean Society of Automotive Engineers
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    • v.17 no.1
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    • pp.182-189
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    • 2009
  • The noise from the sunroof can be divided into the low frequency buffeting noise and the high frequency turbulence noise generated when a car runs at the high driving speed. The wind deflector suppresses the buffeting noise generation by accelerating the vortex shedding from the front edge of sunroof opening, and guides the flow direction so that air can pass smoothly over the sunroof opening. To reduce the buffeting noise and the high frequency noise, it is very important to locate a deflector in a proper position depending on the driving speed and the sunroof opening width. The deflector's sectional shape also plays an important role in efficiently reducing the buffeting and high frequency noise. In this paper, we determined the optimum deflector's sectional shape and examined the flow characteristics behind a sunroof deflector through CFD analysis with changing the deflector height, the driving speed and the sunroof opening width. It is found that the deflector needs to be located in the higher location to control the buffeting noise by shedding the higher frequency vortices to accelerating vortices from the sunroof front edge. The deflector may act as a new noise source at the high driving speed, then it is desirable to put the deflector at the proper height to reduce the flow fluctuations and the noise generation. We also made a road test to verify CFD analysis results in this study.

An study on the Architectural Visual Identity of Apartment Plan (아파트 계획에 있어서 건축적 시각 아이덴티티에 관한 연구)

  • Lee, Dong-Ho;Lee, Seung-Jo;Lee, Kyoung-Hun;Kim, Yong-Seong
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2003.11a
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    • pp.277-282
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    • 2003
  • The purpose of this study is to investigate the architectural visual identity, and scheme of conceptional extent of architectural visual identity through literature research and sample analysis by design factors for apartment design. Korea National Housing Corporation's Mapo apartment put first caterer which is continued about 30 years to solve shortage of houses by beginning and the impact of IMF and the price decontrol on second half in the 1990s have changed the housing status to commercial goods. Construction companies have introduced brand concept to satisfy consumer's appetite in production of house goods including apartment under market situation. Importance of visual identity in apartment design occupies has risen in heftily. However most companies are in condition of stay on two-dimensional application that uses symbol, logotype, and character assistance. Several high position construction companies are trying architectural visual identity, but those are applied in partial element and those are not going forward to systematic classification and synthetic visual identity plan within peculiarity of apartment complex. This study is to grasp plan and design tendency to acquire identity of design element of construction field and goods through design examination. In conclusion visual identity in apartment design is architectural visual identity on all of 2 dimensions and 3 dimension for application. Architectural visual identity is a language of form that prescribe identity about external appearance in combination with brand image.

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The Behavior of Impinging Spray by Piston Cavity Geometry (PistonCavity 형상에 따른 충돌분류의 분무거동)

  • 이상석;김근민;김봉곤;정성식;하종률
    • Transactions of the Korean Society of Automotive Engineers
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    • v.4 no.3
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    • pp.211-219
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    • 1996
  • In a small high-speed D. I. diesel engine, the injected fuel spray into the atmosphere of the high temperature is burnt by go through the process of break up, atomization, evaporation and process of ignition. These process are important to decide the emission control and the rate of fuel consumption and out put of power. Especially, in the case of injected fuel spray impinging on the wall of piston cavity, the geometry of piston cavity gives great influence the ignitability of injected fuel and the flame structure. Ordinary, the combustion chamber of driving engine have unsteady turbulent flow be attendant on such as the change of temperature, velocity and pressure. So the analysis of spray behavior is difficult. In this study, the spray was impinged on the wall of 3 types of piston cavity such as Dish, Toroidal, Re-entrant type, in order to analyze the combustion process of impinging spray precisely and systematically. And hot wire probe was used for analyze non-steady flow characteristics of impinging spray, and to investigate the behavior of spray, the aspects of concentration c(t), standard deviation σ(t) and variation factor(vf) was measured with the lapse of time.

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Fashion Consumption Culture in the Post-COVID-19 Era Identified through Big Data Analysis -Focusing on Articles in the Chinese Fashion Network LADYMAX.cn- (포스트 코로나19 시대의 패션 소비문화에 대한 빅데이터 분석 -중국 패션 네트워크인 LADYMAX.cn의 기사를 중심으로-)

  • Bin, Sen;Yum, Haejung;Shim, Soo In
    • Journal of Fashion Business
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    • v.25 no.2
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    • pp.80-97
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    • 2021
  • In this study, the changes in fashion consumption culture in the post-COVID-19 era were examined through big data analysis. Considering that the Chinese market plays a pivotal role in the global fashion industry, big data was collected in the most famous and professional fashion network in China, LADYMAX.cn. As a result of text mining and social network analysis, three major changes were identified as the emerging fashion consumption culture in the post-COVID-19 era. First, as a trend in new media consumption, COVID-19 disease and the development of digital technology tended to encourage consumers to put more importance on the relationship between bloggers and fans than previously. Second, as a trend in reward consumption, consumers tended to be rewarded for their hard work to relieve and comfort their high stress caused by spending a long time worrying about the prolonged COVID-19 situation. Third, as a trend in home-economy consumption, consumers tended to prefer homewear and sportswear more because they were spending longer times at home as the social distancing period was prolonged.