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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis

MALDI-MS 기반 당단백질 당쇄의 정량분석 기술 개발 연구 동향

  • Kim, Kyoung-Jin (Department of Chemical Engineering, Soongsil University) ;
  • Kim, Yoon-Woo (Department of Chemical Engineering, Soongsil University) ;
  • Hwang, Cheol-Hwan (Department of Chemical Engineering, Soongsil University) ;
  • Park, Han-Kyu (Department of Chemical Engineering, Soongsil University) ;
  • Jeong, Jae Hyun (Department of Chemical Engineering, Soongsil University) ;
  • Kim, Yun-Gon (Department of Chemical Engineering, Soongsil University)
  • 김경진 (숭실대학교 화학공학과) ;
  • 김윤우 (숭실대학교 화학공학과) ;
  • 황철환 (숭실대학교 화학공학과) ;
  • 박한규 (숭실대학교 화학공학과) ;
  • 정재현 (숭실대학교 화학공학과) ;
  • 김윤곤 (숭실대학교 화학공학과)
  • Received : 2014.08.06
  • Accepted : 2015.10.05
  • Published : 2015.10.27
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Abstract

Abnormal glycosylation can significantly affect the intrinsic functions (i.e., stability and solubility) of proteins and the extrinsic protein interactions with other biomolecules. For example, recombinant glycoprotein therapeutics needs proper glycosylation for optimal drug efficacy. Therefore, there has been a strong demand for rapid, sensitive and high-through-put glycomics tools for real-time monitoring and fast validation of the biotherapeutics glycosylation. Although liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the most powerful tools for the characterization of glycan structures, it is generally time consuming and requires highly skilled personnel to collect the data and analyze the results. Recently, as an alternative method, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS), which is a fast, robust and easy-to-use instrumentation, has been used for quantitative glycomics with various chemical derivatization techniques. In this review, we highlight the recent advances in MALDI-MS based quantitative glycan analysis according to the chemical derivatization strategies. Moreover, we address the application of MALDI-MS for high-throughput glycan analysis in many fields of clinical and biochemical engineering.

Keywords

References

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