• Molecules and Cells
• /
• v.39 no.6
• /
• pp.447-459
• /
• 2016

Many studies have been conducted to understand plant stress responses to salinity because irrigation-dependent salt accumulation compromises crop productivity and also to understand the mechanism through which some plants thrive under saline conditions. As mechanistic understanding has increased during the last decades, discovery-oriented approaches have begun to identify genetic determinants of salt tolerance. In addition to osmolytes, osmoprotectants, radical detoxification, ion transport systems, and changes in hormone levels and hormone-guided communications, the Salt Overly Sensitive (SOS) pathway has emerged to be a major defense mechanism. However, the mechanism by which the components of the SOS pathway are integrated to ultimately orchestrate plant-wide tolerance to salinity stress remains unclear. A higher-level control mechanism has recently emerged as a result of recognizing the involvement of GIGANTEA (GI), a protein involved in maintaining the plant circadian clock and control switch in flowering. The loss of GI function confers high tolerance to salt stress via its interaction with the components of the SOS pathway. The mechanism underlying this observation indicates the association between GI and the SOS pathway and thus, given the key influence of the circadian clock and the pathway on photoperiodic flowering, the association between GI and SOS can regulate growth and stress tolerance. In this review, we will analyze the components of the SOS pathways, with emphasis on the integration of components recognized as hallmarks of a halophytic lifestyle.

• Tribology and Lubricants
• /
• v.15 no.3
• /
• pp.248-256
• /
• 1999

Many tribological components in automobile engine undergo high load and sliding speed with thin film thickness. The lubrication characteristics of the components are regarded as ether hydrodynamic lubrication or boundary lubrication, whereas in a working cycle they actually have both characteristics. Many modem engine lubricants have various additives for better performance which make boundary film formation even under hydrodynamic lubrication regime. Conventional Reynolds equation with the viewpoints of continuum mechanics concerns only bulk viscosity of lubricant, which means that its simulation does not give insights on boundary lubrication characteristics. However, many additives of modern engine lubricant provide mixed modes of boundary lubrication characteristics and hydrodynamic lubrication. Especially, high molecular weight polymeric viscosity index improvers form boundary film on the solid surface and cause non-Newtonian fluid effect of shear thinning. This study has performed the investigation about journal bearing system with the mixed concepts of boundary lubrication and hydrodynamic lubrication which happen concurrently in many engine components under the condition of viscosity index improver added.

• Fisheries and Aquatic Sciences
• /
• v.17 no.3
• /
• pp.275-290
• /
• 2014

The marine ecosystem represents a vast and dynamic array of bio-resources attributed with its huge diversity and considered as potential untapped reservoirs for the development of functional foods for future health markets. Basically, marine microorganisms, sponges, algae, invertebrates such as crustaceans and mollusks along with marine fish species can be considered as marine bio-resources, which can be utilized to obtain different health benefits for humans, directly or after processing. Most of the bio-molecular components, such as lipids and proteins from these marine bio-resources, which can be extracted in large scale using the modern and advanced biotechnological approaches, are suitable drug candidates for the pharmaceutical industry as well as functional food ingredients for the food industry. Moreover, the furtherance of high throughput molecular biological techniques has already been incorporated with identification, mining and extraction of molecular components from marine bio-resources. In this review, potential marine bio-resources with respect to their extractable bio-molecules were described in details, while explaining the present and prospective methods of identification and extraction, which are integrated with advanced techniques in modern biotechnology. In addition, this provides an overview of future trends in marine biotechnology.

• Publications of The Korean Astronomical Society
• /
• v.9 no.1
• /
• pp.9-20
• /
• 1994

We observed the molecular transitions of $^{12}CO(1-0)$, $^{13}CO(1-0)$, $C^{18}O(1-0)$, CS(2-1), $HCO^+(1-0)$, and HCN(1-0) toward the high-latitude mole cular cloud MBM12. We derived total $H_2$ column densities for the two velocity components using the optically thin $C^{18}O$ transition. Molecular abundances have been derived for the observed species at the core of this cloud, which appear to be less than an order of magnitude in fractional abundances relative to $H_2$, compared to typical cold dark clouds.

• Food Science of Animal Resources
• /
• v.21 no.3
• /
• pp.246-255
• /
• 2001

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of muscles extracted with distilled water, saline solution, SDS or Trition X-100 showed simular protein patterns between Hanwoo and Holstein meat, indicating that SDS-PAGE technique may not be useful for the identification between Hanwoo and Holstein meat. Lectine blot analysis of muscle extracted with distilled water demonstrated that Hanwoo and Holstein meat had similar affinities for concanavalin A (Con A), ricinus communis agglutinin (RCA-120), ulex europaeus agglutinin (UEA-1) or peanut agglutinin (PNA) lectins. However, approximately 32.1 kDa component of Hanwoo meat showed high affinity for dolichos biflorus agglutinin (DBA) lectin. On the contrary, high molecular weight components of Holstein meat had the specific affinity for wheat germ agglutinin (WGA) lectin. Hanwoo meat-specific components were observed by lectin staining of heat-denatured meat at 100$^{\circ}C$ for 30 sec. Also, the component of heat-denatured meat at 100$^{\circ}C$ for 30 sec, which was slightly smaller than Hanwoo meat-specific component, was concentrated specifically in Holstein meat.

• Mass Spectrometry Letters
• /
• v.13 no.1
• /
• pp.2-10
• /
• 2022

Metabolomics makes it possible to analyze the interrelationships between various signaling molecules based on the metabolic pathways involved by using high-resolution devices. This approach can also be used to obtain large-scale metabolic information to identify the relevant pathways for disease diagnosis and prognosis and search for potential biomarkers. In the fields of medicine and forensics, hair analysis is used to detect various metabolites in the body. Hair can be harvested readily in a noninvasive manner and is easier to transport and store than blood and urine. Another advantage from a forensic viewpoint is that hair reflects all the components of body fluids. In addition, because of the unique coating structure of hair, it can be used for measurements without changing or destroying its adsorbed components. In this review, the pretreatments for hair analysis, instrumental conditions and clinical applications are discussed. Especially, the clinical use of hair metabolomics in the diagnosis of various diseases and the limitations of the technique are described.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.33 no.1
• /
• pp.78-82
• /
• 2020

In this study, MOSFETs fabricated on Si-doped, MBE-grown β-Ga₂O₃ are demonstrated. A Si-doped Ga₂O₃ epitaxial layer was grown on a Fe-doped, semi-insulating 1.5 cm × 1 cm Ga₂O₃ substrate using molecular beam epitaxy (MBE). The fabricated devices are circular type MOSFETs with a gate length of 3 ㎛, a source-drain spacing of 20 ㎛, and a gate width of 523 ㎛. The device exhibited a good pinch-off characteristic, a high on-off drain current ratio of approximately 2.7×10⁹, and a high breakdown voltage of 1,080 V, which demonstrates the potential of Ga₂O₃ for power device applications including electric vehicles, railways, and renewable energy.

• Journal of The Korean Astronomical Society
• /
• v.38 no.2
• /
• pp.237-240
• /
• 2005

We present images of L1551 IRS5 at angular resolutions as high as ${\~}$30 mas, corresponding to a spatial resolution of ${\~}$5 AU, made at 7 mm with the VLA. Previously known to be a binary protostellar system, we show that L1551 IRS5 is likely a triple protostellar system. The primary and secondary components have a projected separation of ${\~}$46 AU, whereas the tertiary component has a projected separation of ${\~}$11 AU from the primary component. The circumstellar dust disks of the primary and secondary components have dimensions of ${\~}$15 AU, whereas that of the tertiary component has a dimension of ${\~}$10 AU. Their major axes are closely, but not perfectly, aligned with each other, as well as the major axis of the surrounding flattened, rotating, and contracting molecular condensation (pseudodisk). Furthermore, the orbital motion of the primary and secondary components is in the same direction as the rotational motion of this pseudodisk. We suggest that all three protostellar components formed as a result of the fragmentation of the central region of the molecular pseudo disk. The primary and secondary components, but apparently not the tertiary component, each exhibits a bipolar ionized jet that is centered on and which emergers perpendicular to its associated dust disk. Neither jets are resolved along their base, implying that they are driven within a radial distance of ${\~}$2.5 AU from their central protostars. Finally, we show evidence for what may be dusty matter streams feeding the two main protostellar components.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.14 no.2
• /
• pp.157-163
• /
• 1985

Various cell wall polysaccharides and related enzyme activities in hot pepper fruit were determined at different stages of maturity. The uronic acid content of cell walls decreased between immature green and turning stage fruit and then increased by red ripe stage. In contrast, cellulose content of cell walls changed only a little during ripening. Total neutal sugar content of cell wall material decreased 50% and galactose content of the walls decreased about 80% by the turning stage. Polygalacturonase and ${\beta}-galactosidase$ activities, as well as total hemicellulose from isolated cell walls of ripening hot pepper fruit were studied using gel filtration chromatography. Polygalacturonase activity was not detectable but 5 isozymes of ${\beta}-galactosidase$ were resolved. The activities of the enzymes were relatively high and gel filtration showed that they differed in molecular weight. Hemicellulose content decreased during ripening and softening. The molecular weight profiles shifted from high molecular weight to low molecular weight polymers during ripening. The changes in cell walls that may be associated with fruit softening involve the alteration of hemicellulose prior to the degradation of wall-bound uronic acid. It is suggested that the decrease in cell wall galactose involved changes in turnover of new cell wall components.

• Journal of Ginseng Research
• /
• v.47 no.2
• /
• pp.228-236
• /
• 2023

Background: QiShen YiQi pills (QSYQ) is a Traditional Chinese Medicine (TCM) formula, which has a significant effect on the treatment of patients with myocardial infarction (MI) in clinical practice. However, the molecular mechanism of QSYQ regulation pyroptosis after MI is still not fully known. Hence, this study was designed to reveal the mechanism of the active ingredient in QSYQ. Methods: Integrated approach of network pharmacology and molecular docking, were conducted to screen active components and corresponding common target genes of QSYQ in intervening pyroptosis after MI. Subsequently, STRING and Cytoscape were applied to construct a PPI network, and obtain candidate active compounds. Molecular docking was performed to verify the binding ability of candidate components to pyroptosis proteins and oxygen-glucose deprivation (OGD) induced cardiomyocytes injuries were applied to explore the protective effect and mechanism of the candidate drug. Results: Two drug-likeness compounds were preliminarily selected, and the binding capacity between Ginsenoside Rh2 (Rh2) and key target High Mobility Group Box 1 (HMGB1)was validated in the form of hydrogen bonding. 2 μM Rh2 prevented OGD-induced H9c2 death and reduced IL-18 and IL-1β levels, possibly by decreasing the activation of the NLRP3 inflammasome, inhibiting the expression of p12-caspase1, and attenuating the level of pyroptosis executive protein GSDMD-N. Conclusions: We propose that Rh2 of QSYQ can protect myocardial cells partially by ameliorating pyroptosis, which seems to have a new insight regarding the therapeutic potential for MI.