• Mass Spectrometry Letters
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• v.4 no.2
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• pp.25-29
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• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• Journal of the Korean Chemical Society
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• v.14 no.4
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• pp.327-332
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• 1970

A simple and convenient device for deflection-type direct reading the variations in electrolytic conductance is described and applied to the analysis of sulfur by combustion-$H_2O_2$ oxidation method. The apparatus consisted of a high resistance-ratio bridge in which the other adjacent arms are the differential cells. By adopting unusually high a-c voltage source for the bridge excitation, the a-c method for unbalanced bridge is established, decreased sensitivity owing to reduced bridge factor, 0.01, is overcome and also the absolute sensitivity and linearity are greatly improved. Over 50% variations in impedance of the balanced cell, within 1% deviation from the linearity can be attained with a volt (rms)order of output which was detected directly with VTVM without further amplification. Analysis of the bridge shows that these useful features are natural result of the constant current character of the high source impedance generator and the performance of the device agreed with the theoretical predictions. A standard procedure for the rapid analysis of sulfur using the bridge is also given, the analytical accuracy was approximately 1%. A determination takes not more than 5 minutes.

• Korean Journal of Pharmacognosy
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• v.50 no.2
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• pp.133-140
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• 2019

In our previous study, we found uracil, adenosine, protocatechuic acid, syringin (eleutheroside B) and scoparone (6, 7-dimethoxycoumarin) in the Oplopanax elatus Nakai Stem. High-performance liquid chromatography (HPLC) -UV was used to quality and quantify the internal marker compounds in the O. elatus extract after validation of method with linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy and precision. The specificity assessment visually confirmed that the substance was detected without the introduction of other substances. The established method showed high linearity of the calibration curve and coefficient of correlation ($R^2$) of over the 0.999. HPLC was reported as five standard compounds equivalent using the following linear equation based on the calibration curve. The accuracy of measurement was 84.34 ~ 119.74% and the relative standard deviation (RSD) value was 0.28 ~ 1.60%. In addition, our established method showed high repeatability. The RSD value was 1.10 ~ 6.81%. So, we found the amount of the internal marker compounds in the O. elatus extract. These results demonstrated that can be used to quality evaluation of the O. elatus.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.9
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• pp.55-59
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• 2008

In this paper, the linearity of Doherty amplifier has been improved by applying a compact slow-wave microstrip branch-line coupler on the output of Doherty amplifier. The proposed branch coupler has four microstrip high-low impedance resonant cells periodically placed inside the branch-line coupler to result in high slow-wave effect. The new coupler not only effectively reduces the occupied area to 30% of the conventional branch-line coupler at 1.8GHz, but also has high second harmonic suppression performance. We obtained the 3rd-order intermodulation distortion ($IMD_3$) of -31.16 dBc for CDMA applications with that of maintaining the constant power added efficiency (PAE). The IMD3 performance is improved as much as -7 dBc compared with a Doherty amplifier.

• JSTS:Journal of Semiconductor Technology and Science
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• v.4 no.4
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• pp.293-299
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• 2004

An active RC 2nd order Butterworth filter suitable for a baseband channel-selection filter of a direct conversion receiver is presented. The linearity of the 2nd order Butterworth filter is analyzed. In order to improve the linearity of the filter, the operational amplifiers should have a high linear gain and low 3rd harmonic, and the filter should be designed to have large feedback factor. This second order Butterworth filter achieves-14dBV in-channel (400kHz, 500kHz) IIP3, +29dBV out-channel (10MHz, 20.2MHz) IIP3 and 15.6 $nV/\sqrt{Hz}$ input-referred noise and dissipates 10.8mW from a 2.7-V supply. The analysis and experimental results are in good agreement

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.2
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• pp.80-85
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• 2010

In this paper, the efficiency of power amplifier has been maximized by the application of EER structure, and the linearity has been improved by using metamaterial structure. This paper has proposed a design of power amplifier in class-F to get the PAE, and to control dynamic power using envelope detector. CRLH structure gets high-linearity by removing harmonics arisen from the mismatching of matching circuit. The PAE and the 3rd order IMD have been improved 5.93 %, 12.83 dB compared with those of conventional Class-F amplifier, respectively.

• Journal of Ocean Engineering and Technology
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• v.15 no.2
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• pp.72-78
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• 2001

The bead is used to provide properly restraining force in the sheet metal forming process. This bead process includes bending and geometrical non-linearity, and affects the state of binderwrap. Therefore, the analysis of bead process is very important to obtain the desired formability. In this paper, the research about the influence of the punch stroke of bead on the draw-bead process was conducted. Results from the analysis will give useful information to the effective tool design of blank forming process. To analyze the bead process, and elasto-plastic finite element formulation is constructed from the equilibrium equation and the considered boundary conditions involved a proper contact condition. The static-explicit finite element method as a numerical method for the analysis was applied to the analysis program code. It was found that this method could solve too much computation time and convergence problem owing to high non-linearity of bead forming process.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.10C
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• pp.993-998
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• 2002

At Power amplifier, distortion of output is appeared because of non-linearlity, so we must study method of compensation for non-linearity. In this paper, it was studied about the characteristic of predistorter using serial schottky diode for an amplifier. As a result, we confirmed that power amplifier was able to linearize when we put predistorter using non-linearity of schottky diode before power amplifier. When input carrier level was low, input carrier was delivered directly into power amplifier but input carrier level was high, input carrier was delivered into power amplifier through predistorter with suppressed level. As a result power amplifier always was at saturation region. Through simulation using serenade 8.0, we have concluded that efficiency was improved about 3%, and predistorter got best linearity at 1.8㎓ between 800㎒∼2.2㎓.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.6 s.121
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• pp.584-590
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• 2007

In this paper, the efficiency of power amplifier has been maximized by the application of EER structure, and the linearity has been improved by using PBG structure. This paper has proposed a design of power amplifier in class-F to get the PAE, and to control dynamic power using envelope detector. PBG structure gets high-linearity by removing harmonics arisen from the mismatching of matching circuit. The PAE and the 3rd order IMD have been improved 34.64%, 6.65 dB compared with those of conventional Doherty amplifier, respectively.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.913-914
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• 2016

This paper analyzes the impact of the satellite TWTA non-linearity charateristics for shared band transmission technology to improve spectral efficiency in satellite communication system. In shared band transmission technology for star network, a hub and terminals use same frequency band. In order to receive terminals's signal a hub have to remove the its own DVB-S2 carrier. In this paper the non-linearity impact of satellite TWTA is analyzed for self-interference cancellation.