• Title/Summary/Keyword: high culture

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Biodegradiation of Benzoate by Pseudomonas sp. (Pseudomonas sp.에 의한Benzoate의 생분해)

  • 김교창;정준영
    • Journal of Food Hygiene and Safety
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    • v.11 no.3
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    • pp.165-170
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    • 1996
  • The biodegradation of high concentration of benzoate by enrichment culture with Pseudomonas sp. was investigated. During 50 days continuous culture, average of removal rate of benzoate and COD were 90% and 83%, respectively. And the enzymatic activity of catechol 2,3-dioxygenase was determined in the continuous culture but not Catechol 1,2-dioxygenase. On the other hand, Pseudomonas sp in the culture was investigated with SEM and the result was revealed that the cell shape was more demage according concentration of benzoate.

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Continuous high cell density culture of Anaerobiospirillum succiniciproducens with membrane filtration for the production of succinic acid

  • Lee, Pyeong-Cheon;Lee, U-Gi;Lee, Sang-Yeop;Jang, Ho-Nam
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.338-341
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    • 2000
  • An internal membrane bioreactor system was employed for continuous succinic ac id production from glucose in order to prove its performance and practicality. Succinic acid-producing Anaerobiospirillum succiniciproducens required more $CO_2$ for the proper growth and succinic acid production in cell recycled continuous culture than in batch culture. The maximum productivity obtained in cell recycled continuous culture was about 3.3 g/L-h which was ca. 3.3 times higher than that obtained in batch culture.

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Improvement of Plants by Biotechnology (세포공학을 이용한 식물개량)

  • 윤의수
    • Korean Journal of Plant Resources
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    • v.3 no.1
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    • pp.1-30
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    • 1990
  • The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

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Removal of High Odor Concentration with Biofilter using Mixture of Earthworm Cast and Distillery Sludge (지렁이 분변토와 주정슬러지 혼합 배양액을 이용한 Biofilter에서의 고농도 악취제거)

  • Park, Jong-Woong;Jang, Seg-Joo
    • Journal of Environmental Health Sciences
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    • v.40 no.2
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    • pp.127-136
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    • 2014
  • Objectives: This study was conducted to investigate the removal of high odor concentration from swine wastewater treatment facility by full scale biofilter using liquid with deodorant mixed with earthworm cast and distillery sludge. Methods: The supply of the culture liquid to the microorganism on the media in the biofilter increases the activity and growth of biomass. The experimental equipment was biofilter tower with treatment capacity of 90 m 3/min. The experimental conditions included gas flow of $60m^3/min$, retention time of 20 sec, and gas/liquid ratio of 67. Results: With changing season from winter to summer, the inlet odor concentration of ammonia increased from 2.5 ppm to 29 ppm, and of hydrogen sulfide from 21 ppm to 91 ppm, respectively. The odor treatment system with biofilter using the culture liquid was stable when the high loading rate increased and showed excellent removal grade with an average of 96.7% for ammonia, and an average of 93.7% for hydrogen sulfide. The pH and SCOD in the recirculating culture liquid near the bottom of the biofilter tower decreased with operation time, but its influence on the odor removal rate was negligible, because the organic matter (SCOD) was replaced by some culture liquid supplied 2-4 times per day. Conclusions: The biofilter using culture liquid could successfully remove high odor concentration which was generated from swine wastewater treatment facility.

Systematic Propagation of High Quality Garlic (Allium sativum L.) Through Shoot Apical Meristem Culture III. Micropropagation by Involucre Culture (생장점배양에 의한 우량 마늘의 체계적 증식 III. 총포배양에 의한 무병주 대량증식)

  • Lee, Eun-Mo;Lee, Young-Bok
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.5
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    • pp.277-280
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    • 1994
  • This study was conducted to obtain some basic information needed for the propagational system of high quality garlic trough the culture of healthy tissues. non shoot-tips of bulbil obtained in mid May were cultured on MS medium containing 8% sucrose supplemented with 0.1 mg/L NAA, in vitro bulbli were formed, but the shoots were formed at the early to middle in June. Multiple shoots were induced by the culture of receptacles on MS medium supplemented with 0.1 mg/L NAA and 10mg/L BA..Among the flower bud, bulbil and receptacle, receptacle showed most suitable in terms of shoot formation efficiency, More than 50 shoots per single involucre were produced under the optimum condition. Results indicate that in vitro culture of involucre has a high potential for the micropropagation of high quality seed bulbs.

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High-Level Production of Astaxanthin by Fed-Batch Culture of Mutant Strain Phaffia rhodozyma AJ-6-1

  • KIM, SU-JIN;GEUN-JOONG KIM;DON-HEE PARK;YEON-WOO RYU
    • Journal of Microbiology and Biotechnology
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    • v.13 no.2
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    • pp.175-181
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    • 2003
  • The production of a carotenoid astaxanthin, a growth-associated principal pigment, is limited in a batch cultivation, because a high glucose concentration severely inhibits the cell growth and also influences the carotenoid production. Therefore, a fermentation strategy including effective chemicals for the high-level production of cells and astaxanthin by a mutant strain Phaffia rhodozyma AJ-6-1 was developed in a fed-batch culture. First, a production medium for maximizing the cell and astaxanthin yields was formulated and optimized. Using this optimized medium, the highest cell and astaxanthin concentrations obtained were about 38.25 g/1 and 34.77 mg/1, respectively. In addition, an attempt was made to increase the amount of astaxanthin using effective chemicals such as ethanol and acetic acid, which are known at an inducer and/or precursor of carotenoid synthesis. When either 10g/1 ethanol or 5 g/1 acetic acid was added to investigate the resulting astaxanthin content, a relatively high astaxanthin concentration or 45.62 mg/l and 43.87 mg/1, respectively, was obtained, and the cell concentrations also increased slightly under these conditions. Therefore, these results imply that a fed-batch culture of the mutant strain P. rhodozyma AJ-6-1 could be effectively employed in the commercial production of astaxanthin, although the factors affecting the productivity remain to be elucidated.

The Effectiveness of Jeju Island Mixed Reality Tourism Application: Focusing on the Moderating Role of Users' K-Culture Involvement

  • Yoo, Seungchul;Jung, Kwanghee;Nguyen, Vinh T.;Piscarac, Diana
    • International Journal of Advanced Culture Technology
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    • v.8 no.2
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    • pp.116-128
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    • 2020
  • In this study, we introduce a multi-modal mixed reality (MR) application for advertising the main touristic landmarks on Jeju Island, and evaluate its effectiveness based on the Technology Acceptance Model (TAM) with a focus on the user's involvement in Korean culture (K-Culture). Specifically, we deployed several stimuli to enable users to retrieve information, visualize a variety of heritage content such as text, images, videos, virtual artifacts, and immerse in a VR environment to experience the heritage locations. The results of the online survey with 436 participants showed that for consumers with low K-Culture involvement, Perceived Usefulness and Perceived Ease-of-Use did not affect their Intention to Visit the island. Still, it had a significant impact on their Intention to Use the application. On the other hand, for the group with high K-Culture involvement, Perceived Usefulness had no effect on the Intention to Use and Intention to Visit. However, Perceived Ease-of-Use had a significant impact on their Intention to Use the application and Intention to Visit Jeju Island. Therefore, when user involvement in K-Culture is high, convenience of use has played a significant role in sensory media usage and the intention to visit, regardless of the usefulness of the mixed reality content. In contrast, users with low K-Culture involvement primarily focused on the functional aspects of the application. These findings open the path for follow-up studies on K-Culture involvement and immersive media tourism marketing, which will benefit digital tourism marketing and Korea's tourism industry.

Cross-cultural Research on Visual Values of Korean and Japanese Private Girl's High School Uniform -Focused on Private Girl's High School Uniform in Seoul and Tokyo- (한.일 사립 여고생 교복의 조형성에 관한 비교 문화 연구 -서울과 도쿄의 사립 여자 고등학교 교복을 중심으로-)

  • Yun, Su-Jeong;Kwon, Ha-Jin;Kim, Min-Ja
    • Journal of the Korean Society of Costume
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    • v.57 no.7
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    • pp.30-44
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    • 2007
  • The culture includes every possible objective forms created by humans and their shared aspects of ideas, feelings, and behaviors, beliefs and etc. The dress and its ornaments are part of the culture that expresses the relationships in objective ways and in formless immaterial ways. The school uniform represents significant meanings to the students in their school years, and it is the way of expressing their individuality and full filling their needs for self-presentation. Therefore, understanding and analyzing youth culture means understanding students' school uniform along with fast changing twenty first century civilization. Korea and Japan are very close nations to each other. Both Korean and Japanese girls' school uniform style were adopted western clothing style and went through many changes based on cultural differences between two countries. They are affected by different peculiarities between two nations and youth cultures from different cities. Therefore, this research is on analyzing how different visual values of girl's high school uniforms in Seoul and Tokyo and understanding youth cultures through macro-analysis. The youth cultures in Korea and Japan are mostly similar but there is special development on animation in Japan and possibly can find many styles of girl's high school uniform. Both nations are varying to be opened to sexual culture of adolescents but Japan is more uninhibited than Korea. In Japan, sailor-uniform can be found in fetish way in public culture. On one hand, because of most private girl's high schools in Tokyo takes an entrance examination, top-ranking students are very proud of their school uniforms and even students from other public high schools get private high school uniforms to attend school festival. This analysis showed that private girl's high school uniform in Seoul appealed as slim fitted silhouette which is close to modem woman's suit style while in Tokyo appealed as boxy style matched with short pleated skirt or sailor-uniform style. Comparing that to school uniform photos taken on the street, we can find that alikeness or more extreme styles as examples. These are influenced by different youth cultures in Seoul and Tokyo and cultural differences stands for different aesthetic norm being accepted or rejected.

study on the Transfeal Phenomenon of Anti-fashion into Mother-fashion -In the Case of Hippie Look- (저항패션이 모패션으로 전이된 현상에 관한 연구 -히피풍을 중심으로-)

  • 박명희
    • Journal of the Korean Society of Costume
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    • v.28
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    • pp.239-253
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    • 1996
  • The purpose of this dissertation is to exam-ine the hippie look which is one of the most popular fashion trends in the early 90s, in re-lation to the hippie style in 60s and cultural restoration. in order to investigate the re-lationship this dissertation studies the trans-feral phenomenon of anti-fashion into mother fashion in the basis of social and clutural theories. hippie culture is the anti-culture which appeared in America in the late 60s, it tried to create a new culture by subverting the ruling class culture with their combative life style and peculiar appearance which are distinguished from mother culture. First of all to examine the inner symbolic meaning and outer shape of the hippie style I divided the characteristics of the hippies into ideology social value and attitude on sex. in the aspect of ideology the hippie show strong subjectivity while mother culture shows ob-jectivity. in social value the hippie has the resisting chaacteristics while mother culture has the conformative characteristics. In the aspect of sex the hippie tries to break the fixed idea of mother culture. Hippie culture as anti-culture is symbolically expressed in hippie style fashion which is dif-ferent from mother fashion in shape. There-fore the inner meaning and the outer shape of the hippie style shows strong characteristics of anti-fashion. Hippie style as anti-fashion has an effect on high fashion in the late 60s and the early 70s and on hippie look which is restored in 90s. however hippie-look in 90s is a mother-fashion in which all symbolic mean-ings of the hippie style are weakened and the only outer shape of hippie style is presented and suggested. It doesn't show any cultural backgrounds ideology social value and new attitude on sex of the hippies. The transferal phenomenon of anti-fashion into mother-fashion is summarized as follows. First in the process of tranference to highfashion in 60s and 70s and hippie-look in 90s the resistance of the hippie style is weakened and only suggested through the outer look. Second the hippie style which reappeared in the high fashion and mother fashion in 90s are layered look military uniform mode flower print grunge look romantic look peasant look ethnic look gypsy style handicraft ornaments working suit style handicraft ornaments working suit style and the use of the see-through cloth. Third the fact that the popular and high fashion were influenced by anti fashion in 60s proves filter-up theory in fashion adaption theory. As I examined before the ideology of the hippies are faded in high fashion in 60s-70s and hippie-look in 90s. The symbolic meaning of the hippie style is also weakened and only presented as a fashion trend.

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