• Title/Summary/Keyword: high culture

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Residential Values Analyzed in Daily Newspapers (주요일간지의 아파트관련 기사에 나타난 주거가치 특성에 관한 연구)

  • Lee, Sung-Mi;Lee, Yeun-Sook
    • Proceedings of the Korean Institute of Interior Design Conference
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    • 2004.05a
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    • pp.91-94
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    • 2004
  • The purpose of this study is to inquire the change of residential value in the phenomena related with apartment living culture for recent 10 years. To investigate the apartment culture and residential values, articles in daily newspapers, which is related to 'apartment,' has been analysed by theme divided into physical, behavioral, valuable aspects. As a result, it han found that there were higher concern about economic value the any other values. Also, some of recent trands was found out in this study, such as, high quality, diversification, identity, healthy life, etc. Here this study is purposed to serve exploring the basis of healthier living culture for the future.

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Plasmid Stability and Cloned-Gene Expression in Continuous Culture of Recombinant Escherichia Coli Under Derepressed Condition

  • Nam, Soo-Wan;Kim, Byung-Kwan;Kim, Jung-Hoe
    • Journal of Microbiology and Biotechnology
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    • v.4 no.1
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    • pp.1-6
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    • 1994
  • Continuous culture was carried out with a recombinant Escherichia coli W3110/pCR185, which encodes trp-operon enzymes when the temperature is shifted from $37^{circ}C\;t;42^{\circ}C$. Under derepressed condition of $42^{\circ}C$. plasmlid stability and gene expression were analysed as function of the dilution rate. The stability of plasmid increased with the dilution rate, but maximal levels of gene expression (tryptophan concentration) and plasmid DNA content were obtained at the lowest dilution rate, $0.075\;hr^{-1}$. The plasmid instability, observed at low dilution rates, could be explained by the unbalanced biosynthetic state of the recombinant cell harboring a high copy number of plasmid.

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Production of ${\gamma}-Aminobutyric$ Acid (GABA) by Lactobacillus buchneri Isolated from Kimchi and its Neuroprotective Effect on Neuronal Cells

  • Cho, Yu-Ran;Chang, Ji-Yoon;Chang, Hae-Choon
    • Journal of Microbiology and Biotechnology
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    • v.17 no.1
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    • pp.104-109
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    • 2007
  • Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.

Hypolipidemic Effect of Exo- and Endo-Biopolymers Pmduced from Submerged Mycelial Culture of Ganoderma lucidum in Rats

  • Yang, Byung-Keun;Jeong, Sang-Chul;Song, Chi-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.12 no.6
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    • pp.872-877
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    • 2002
  • The hypolipidemic effect of the exe-biopolymer (EXBP) and endo-biopolymer (ENBP) produced from a submerged mycelial culture of Ganoderma lucidum was investigated in dietary-induced hyperlipidemic rats. Hypolipidemic effects were achieved in both the EXBP- and ENBP-treated groups, however, the former proved to be more potent than the latter. The administration of the EXBP (100 mg/kg body weight) substantially reduced the plasma total cholesterol, low-density lipoprotein (LDL) cholesterol, triglyceride, phospholipid levels, and atherogenic index by 31.0, 39.0, 35.4, 28.1, and 53.5%, respectively, when compared to the control group. The EXBP also lowered the liver total cholesterol, triglyceride, and phospholipid levels by 22.4, 23.1, and 12.9%, respectively. Furthermore, the high-density lipoprotein (HDL) cholesterol and ratio of HDL cholesterol to total cholesterol were significantly increased by as much as 24.2% and 47.6%, respectively.

Legal Culture and Commercial Arbitration in the United States and Japan

  • Kim, Chin-Hyon;Chung, Yong-Kyun
    • Journal of Arbitration Studies
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    • v.23 no.3
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    • pp.185-212
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    • 2013
  • In this paper, a conceptual model of legal culture based on Ehrlich's "living law" theory and Cole's social-cultural explanation can explain the low utilization rates of arbitration of Japan and the high utilization rates of arbitration in the United States, simultaneously. This model highlights the clash between social norms and legal provisions in Japan. Japan has developed a two-tiered system of dispute resolution. At the official level, Japanese people accept the legal system imposed by the outside world. But, at a deeper level, they utilize diverse forms of informal dispute resolution mechanisms, such as reconcilement and conciliation, reflecting their own social norms. In contrast, there is no conflict between social norms and legal provisions in United States. This study may show that there are distinctions between American-style arbitration and Japanese-style arbitration, reflecting their own respective social norms. The question of reconciliation between the American style of arbitration and the Japanese style of arbitration can be resolved by an international arbitrator.

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Isolation and Its Optimal Culture Condition for High Agarase-Producing Mutant (한천분해효소의 고생산성 변이주의 분리 및 최적배양조건)

  • 황선희;하순득;김봉조;김학주;공재열
    • KSBB Journal
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    • v.14 no.3
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    • pp.351-357
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    • 1999
  • A marine bacterium Bacillus cereus ASK202, agarase producing strain, was treated with some mutagenic agents, ultraviolte(UV), 1-methyl-3-nitro-1-nitrosoguanidine(NTG), and ethyl methane sulfonate(EMS), several times for the increasing of the agarase production After mutagen treatment, we isolated one mutant strain treated with NTG showed the highest stability and agarase productivity and named as Bacillus cereus ASK202-N3. This Bacillus cereus ASK202-N3 strain was well grown in the modified marine medium containing 0.5%(w/v) agar, 0.3%(w/v) yeast extract, and 5.0%(w/v) NaCl, and the optimal initial pH, temperature and culture time were 7.8, $25^{\circ}C$ and 32h, respectively. In the optimal culture conditions, the agarase production was increased to 5.3 fold(850units/L) compared to that of the wild type.

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A Negative Effect of Animation Character in Mass Culture of Korea (한국의 대중문화에 Animation Character가 미치는 부정적인 영향)

  • 김홍산;신정숙
    • Archives of design research
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    • v.14 no.2
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    • pp.147-154
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    • 2001
  • The Animation Character is high value-added industry in an aspect of economic value. Because Animation Character creates various synergic effects. First and most importantly, the value of animation character is role of communicator in mass culture. The diverse personality and behavior of Animation character affects masses's a way of thinking and act. But the personality and behavior of Animation character express an extreme inclination, for the most part. And presentation of role's model that is necessary for maintenance and continuance of mass society is Auctioning wrongfully.

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Isolation of a Pestalotiopsis Species Degrading Mucilage from Fruit of Opuntia ficus-indica var. Saboten

  • Huh, Yoon-Hee;Ko, Young-Hwan
    • Journal of Applied Biological Chemistry
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    • v.50 no.4
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    • pp.221-226
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    • 2007
  • The high molecular-weight mucilage extracted and purified from cactus fruit of Opuntia ficus-indica var. Saboten was degraded by the cell-free culture filtrate of a fungus isolated from soil. TLC analysis of the polymeric mucilage after incubation with the fungal culture filtrate confirmed its degradation. When the degradation products were tested for their qualitative reactions with ninhydrin and phenol-sulfuric acid, only phenol-sulfuric acid gave positive development, and ninhydrin did not show any observable color reaction. This coloring reaction suggested the presence of a carbohydrate without an amino group within the mucilage. Analyses by HPLC and liquid gel permeation chromatography on sephadex G-100 also provided additional information on degradation of the mucilage by the fungal culture filtrate. The sequences of ITS-5.8S rDNA from the fungal isolate that was cultivated for the preparation of mucilage-degrading enzyme showed 99% similarity to those of Pestalotiopsis aquatica.

Cultural characterization of probiotic Lactobacillus sakei BK19

  • Yang , Byung Gyoo;Song , Choon Bok;Yeo , In Kyu;Lee , Kyoung Jun;Park , Geun Tae;Lee, Sang Hyeon;Son, Hong Joo;Heo, Moon Soo
    • Journal of fish pathology
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    • v.16 no.2
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    • pp.119-123
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    • 2003
  • We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.

Characteristics of Culture for Emulsive Biosurfactant-Strain from the Soil (토양으로부터 분리한 유화성 생체계면활성 균주의 배양 특성)

  • 임윤택;윤용수
    • Journal of environmental and Sanitary engineering
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    • v.11 no.3
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    • pp.69-77
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    • 1996
  • The result of isolated and selected to the strain having the emulsifying activity from soil's strain the strain was identified as Candida genus. The strain was investigated with culture condition at pH culture temperature, flow rate of air, strring rate etc., and physicochemical properties of the biosurfactant were examined. The optimum composition of medium for a strain cultivation were obtained as follow : glucose ; 100g/L, yeast extract ; 10g/L, urea ; 1.0g/L, KH$_{2}$PO$_{4}$ ; 50mg/L, MgSO$_{4}$ ; 500mg/L, and the op condition of cultivation was as follow : pH ; 3.0, temperatlue ; 24$\circ $C, strring rate ; 40rpm. The maximum yield of biosurfactant was obtained by pH ; 3.0-3.5, and temperature ; 25$\circ $C. The degree of emulsification of syntesized biosurfactant was increased clearly by increasing concentration of biosurfactant and it's stability was maintained for a long time. The surface tension of biosurfactant was varied with pH, especially it was showed that the surface tension was high at acidic pH.

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