• Title/Summary/Keyword: high culture

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Effects of Addition of Pichia anomala SKM-T and Galactomyces geotrichum SJM-59 on Baechu Kimchi Fermentation (Pichia anomala SKM-T와 Galactomyces geotrichum SJM-59 첨가가 배추김치 발효에 미치는 영향)

  • Mo, Eun-Kyoung;Ly, Sun-Yung;JeGal, Sung-A;Sung, Chang-Keun
    • Food Science and Preservation
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    • v.14 no.1
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    • pp.94-99
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    • 2007
  • To investigate the effects of Pichia anomala SKM-T and Galactomyces geotrichum SJM-59 on Baechu kimchi fermentation, lyophilized yeasts were added to Baechu kimchi and co-cultured at room temperature ($20{\pm}2^{\circ}C$) for 7 days. Desirable pH and acidity levels appeared by 3 days of fermentation in both the control culture and that with added G. geotrichum SJM-59. Furthermore, the culture with G. geotrichum SJM-59 sustained a desirable pH and acidity level until 5 days of co-culture. The pH of the culture with P. anomala SKM-T decreased slowly and was significantly higher than that of control throughout the experimental period. As fermentation time increased, the acidity of the culture with P. anomala SKM-T increased gradually. However, this culture maintained a desirable acidity level throughout the experiment. The number of lactic acid bacteria in the culture with P. anomala SKM-T was higher than in the culture with G. geotrichum SJM-59, or the control culture, throughout the experiment. The highest LA/TM ratio appeared after 3 nays of fermentation in the control culture, and on the 5 day of the yeasts added co-cultures. On sensory evaluation, no differences were detected between control and the culture with G. geotrichum SJM-59 arter 3 days of fermentation. The co-cultures with yeasts received high marks in umami taste. The co-culture with P. anomala SKM-T showed better texture properties than did the control culture. It was considered that fermentation times were delayed by addition of G. geotrichum SJM-59 or P. anomala SKM-T to Baechu kimchi fermentation.

Construction of the Honam Culture Information System(HCIS) using Web GIS (WebGIS를 이용한 호남문화정보시스템(HCIS) 구축)

  • Yang, Hea-Kun;Shin, Hye-Jin
    • Journal of the Korean association of regional geographers
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    • v.12 no.2
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    • pp.291-304
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    • 2006
  • Individual culture information has been the mainstream in studies on culture information so far, and the studies have focused on zones using paper map. As a result, intuitive analysis in map and extremely restricted measuring space analysis are limited in summarizing and utilizing complicated and huge cultural materials systematically and scientifically. Introduction of GIS can be regarded as an indispensable element for solution of this problem as it can analyze temporal-spatial dynamics of culture information as a whole and to construct effective management system for regional culture information. In particular, supply of two-way information rather than one-way information becomes more and more important in the society structure where value is diversified and the culture gets faster owing to high-level information industry like today. Accordingly, this study is considered to be meaningful in that WebGIS-based regional culture information system allows temporal-spatial analysis and spatial analysis for various culture information for the users using internet. Regional culture information system like culture information system in Honam region can not only contribute to comparative study between regions and to creation of new information through analysis of statistics between culture elements but also allow easy and comprehensive approach to regional information.

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Effect of Glycine Betaine on Follicle-Stimulating Hormone Production by Chinese Hamster Ovary Cells at Low Culture Temperature (CHO 세포의 저온배양에서 Glycine Betaine이 재조합 FSH의 생산에 미치는 영향)

  • Yoon, Sung-Kwan;Ahn, Yong-Ho
    • KSBB Journal
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    • v.22 no.2
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    • pp.109-113
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    • 2007
  • Suspension culture of recombinant Chinese hamster ovary (CHO) cells producing follicle-stimulating hormone was performed to investigate the effect of glycine betaine on cell growth and FSH production at low culture temperature. At 28$^{\circ}C$, cell growth was suppressed, but cell viability remained high for a longer culture period. When the culture temperature was lowered from 37$^{\circ}C$ to 28$^{\circ}C$, more than 14-fold increase in the maximum FSH titer was achieved. In batch culture at 28$^{\circ}C$, the use of 15 mM glycine betaine (GB) to culture medium resulted in the enhancement of maximum cell density and FSH titer by 11% and 17%, respectively, compared to the culture without GB. In pseudo-perfusion culture at 28$^{\circ}C$ with the exchange of fresh medium containing 15 mM GB, a final FSH of $2,058{\mu}g$ which is approximately 1.4-fold higher as compared to the culture without GB was obtained. This enhanced FSH production with 15 mM GB was not just because of enhanced specific FSH productivity (qFSH), but mainly because of the extended culture longevity. Taken together, this result demonstrates that the application of GB at low culture temperature is feasible to enhance the production of recombinant proteins in rCHO cells.

Analysis on Organization Performance Based on Hospital Culture (기독병원과 일반병원의 조직문화 특성에 따른 조직성과 분석)

  • Kim, Woon-Shin;Nam, Eun-Woo
    • Korea Journal of Hospital Management
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    • v.4 no.2
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    • pp.242-265
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    • 1999
  • Our study attempt is to see organizational performance according to the differences between types of hospital cultures. To determine theoretical relationship between the organizational culture and the performance, we select two hospitals in Pusan which are different in the purposes and shapes of establishment. We surveyed their members on a questionnaire based on the type of the organizational culture of the two institutions, analyzed, and review its organizational effectiveness. For the survey with questionnaires, which had been preliminary studied to raise its validity, question forms were distributed to 528 persons in April, 1999 based on the self-responses and recollected within 48 hours. The recollection rate was high(89.96%) and the quantity of questionnaires used for our final analysis was 430(81.44%). The Cronbach Coefficient Alpha of the questionnaires was 0.742. Regarding statistical techniques for analysis of the written materials, dispersion analysis(ANOVA) was adapted to test the organizational effectiveness of the two hospitals having the different organizational cultures, and Pearson Correlation was applied to determine correlations was among all variables. T-test was performed to test organizational effectiveness based on the differences in the extent of sharing the culture, organizational committment and work satisfaction between the two health institutions. From our analysis, we obtain the following conclusions. First, concerning with organizational culture of the two hospital, one of which is a christian hospital and the other is a private foundation hospital, the former is conservative and human-oriented but the latter focuses on renovation and accomplishment. Second, the private establishment has a relatively higher organizational effectiveness that the religious hospital as a result of analyzing the extent of sharing culture, organizational committment and work satisfaction. Third, it has been found that the correlations between the extent of the sharing culture and the organizational committment, the extent and work satisfaction, and the committment and the satisfaction are respectively positive influencing organizational effectiveness, especially work satisfaction. Fourth, cultural factors by which the christian hospital is affected more positively including human relations among its members, belief, its idea of establishment, tradition, work responsibility, power, and wage. On the other hand, factors such as director's leadership, personnel management, wage, hospital regulations and department managers' management ability have been seen as negative influences in order. And fifth, for the private foundation hospital human relations among its members, wages, work responsibility, director's leadership and department managers' management ability were positive in their sequence while wages, personnel management, hospital regulations, welfare and department managers' management ability were considered as negative influences in order. As these results of this study, the higher extent of sharing organizational culture, the more increasing in both organization committment and work satisfaction, the higher the effectiveness. Although it was somewhat difficult to generalize the results whose subjects were the two hospitals only, it was obvious that organizational culture was an important influential factor of organizational effectiveness. It is questionable that the extent of sharing organizational culture, organizational committment and work satisfaction as variables affecting the effectiveness have their validity, but this study has its significance in that it provided an approaching to evaluate the organizational culture of individual hospitals making allowances for such variables related to the general activities in its hospital. We hope the results of the study could be useful for the managerial strategies of the institutions.

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The impact of post-warming culture duration on clinical outcomes of vitrified-warmed single blastocyst transfer cycles

  • Hwang, Ji Young;Park, Jae Kyun;Kim, Tae Hyung;Eum, Jin Hee;Song, Haengseok;Kim, Jin Young;Park, Han Moie;Park, Chan Woo;Lee, Woo Sik;Lyu, Sang Woo
    • Clinical and Experimental Reproductive Medicine
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    • v.47 no.4
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    • pp.312-318
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    • 2020
  • Objective: The objective of the study was to compare the effects of long-term and short-term embryo culture to assess whether there is a correlation between culture duration and clinical outcomes. Methods: Embryos were divided into two study groups depending on whether their post-warming culture period was long-term (20-24 hours) or short-term (2-4 hours). Embryo morphology was analyzed with a time-lapse monitoring device to estimate the appropriate timing and parameters for evaluating embryos with high implantation potency in both groups. Propensity score matching was performed to adjust the confounding factors across groups. The grades of embryos and blastocoels, morphokinetic parameters, implantation rate, and ongoing pregnancy rate were compared. Results: No significant differences were observed in the implantation rate or ongoing pregnancy rate between the two groups (long-term culture group vs. short-term culture group: 56.3% vs. 67.9%, p=0.182; 47.3% vs. 53.6%, p=0.513). After warming, there were more expanded and hatching/hatched blastocysts in the long-term culture group than in the short-term culture group, but there was no significant between-group difference in embryo grade. Regarding pregnancy outcomes, the time to complete blastocyst re-expansion after warming is shorter in women who became pregnant than in those who did not in both culture groups (long-term: 2.19±0.63 vs. 4.11±0.81 hours, p=0.003; short-term: 1.17±0.29 vs. 1.94±0.76 hours, p=0.018, respectively). Conclusion: The outcomes of short-term culture and long-term culture were not significantly different in vitrified-warmed blastocyst transfer. Regardless of the post-warming culture time, the degree of blastocyst re-expansion 3-4 hours after warming is an important marker for embryo selection.

Safety Assessment of the Level of Safety Culture of National Critical Infrastructure Expressway Operating Organizations (국가핵심기반 고속도로 운영기관의 안전문화 수준진단에 관한 소고)

  • Seo, Jeong-soo;Cheung, Chong-soo
    • Journal of the Society of Disaster Information
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    • v.18 no.3
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    • pp.636-645
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    • 2022
  • Purpose: This study intends to suggest alternatives for improving the level of safety culture by measuring/analyzing safety culture targeting employees of national core-based highway operating organizations. Method: Using the 'Safety Awareness Level Diagnosis Tool' of the Korea Occupational Safety and Health Agency, 16 sub-factor measurement tools reflecting 4 safety culture areas and 4 safety culture activities were evaluated for a total of 144 items. were surveyed/analyzed by online questionnaire. Result: As for the results by safety culture area, "safe operation" was the highest, and "safe communication" was the lowest. As for the results of each safety culture activity, "safe execution (D)", which evaluates whether the plan was implemented, was high. The lowest level of safety culture is "Safety Improvement (A)" Conclusion: When establishing a company's safety and health management measures, the most important aspect of management is the level of safety culture. The ultimate goal is to improve the level of safety culture. In this study, it was possible to confirm the safety culture level of the national core-based expressway operating institution. In the future, we intend to conduct a study on how safety culture affects business continuity management system (BCMS).

Effects of Soil Moisture Control and Truss Limited High Density Culture on the Growth and Yield of Tomato(Lycopersicon esculentum Mill. cv. Momotaroyork) (토양수분조절과 저단밀식재배가 토마토의 생육 및 수량에 미치는 영향)

  • Kim, Gi-Don;Yoon, Wha-Mo
    • The Journal of Natural Sciences
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    • v.14 no.2
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    • pp.103-114
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    • 2004
  • This study aims at investigating the effect of soil moisture control and truss limited high density culture on the growth, fruit yield and quality of tomato. To minimize of loss yield of tomato, flower cluster in number was limited to two and three truss and planting density was raised. Soil moisture control was started from 40 days after anthesis and irrigation point was set in -30kPa and -50kPa, which were compared with -10kPa For high density culture, the planting number of truss limited high density culture was planted twice as many as control. Soil moisture repression reduced the growth of stem diameter, leaf and plant height. Leaf chlorophyll content was higher in -50kPa and -30kPa than control. No significant differences, however, shows in -10kPa. The occurrence rate of bloom-end rot and cracking was increased by growing of irrigation repression. Pinching three fruit truss was higher than pinching two fruit truss in the occurrence rate of them. Soil moisture repression resulted in the reduction of fruit weight and in special, truss limited high density was distinctly decreased in -50kPa. The number of fruit was not affected by soil moisture control, but 3rd flower cluster was lower than 2nd flower cluster in the number of fruits and 2nd one was lower than 1st one. Under irrigation repression, rate of dry matter tended to grow in -30kPa, -50kPa compared with control and pinching three fruit truss was higher than two truss. Marketable yield dropped to 36.7%m 46.3 in -30kPa, -50kPa on pinching two fruit truss and dropped to 27.3%, 32.3% in 3rd flower cluster compared with control.

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The Bi-polarized Consumption and Policy Reponses in China (중국의 소비 양극화와 정책 대응)

  • Lee, Jung Hee
    • Journal of International Area Studies (JIAS)
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    • v.13 no.2
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    • pp.315-338
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    • 2009
  • The purpose of this paper is to analyze the situation of bi-polarized consumption in China before and after the global financial crisis, to find the factors causing bi-polarized consumption, and to suggest some policies for Korean enterprises. The findings are as follows. First, before the global financial crisis, in China, there were bi-polarized consumption among classes, and between urban and rural areas, and bi-polarization of individual consumption. The sales of both low price goods and high price goods increased more than that of middle price goods. Second, after the global financial crisis, the trend of bi-polarized consumption in China is stronger than those of other countries. The sales of both low price goods and high price goods in China increase more than in other countries. Third, the factors causing bi-polarized consumption are bi-polarized income, black and grey incomes, "Mianz" culture, the increase of unemployment, and the changing form of family. Especially, the level of formal income is not only high, but the level of black and grey income is also very high. And "Mianz" culture means the conspicuous consumption. The degree of the conspicuous consumption of China is very high in comparison with other countries because "Mianz" culture is strong. Finally, the paper suggests strategies appropriate for bi-polarized consumption with Chinese characteristics.

Effects of Emotional Labor, Nursing Organizational Culture on Self-efficacy in Clinical Nurses (임상간호사의 감정노동과 간호조직문화가 자아효능감에 미치는 영향)

  • Kwon, Myoung-Jin;Kim, Keum-Sook;Ahn, Sung-Yun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.4
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    • pp.2225-2234
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    • 2014
  • The objective of this study was to the effects of emotional labor and nursing organizational culture on the self-efficacy among clinical nurses. The subjects of this study were the clinical nurses working at 6 general hospitals in C & D city. The self-reported questionnaires were administered to 475 clinical nurses. The levels of self-efficacy, nursing organizational culture and emotional labor of the subjects were revealed moderate to high. Self-efficacy and emotional labor, emotional labor and nursing organizational culture showed a negative correlation, Self-efficacy and nursing organizational culture showed a positive correlation. Nursing organizational culture affects the self-efficacy that is significant explanatory variables, of which variables are in general education, job satisfaction, and employee turnover experience. The findings suggested that Nurses' self-efficacy for managing environmental management programs with interventions seem to be necessary.

Anther Culture Efficiency According to Plating Method in Naked Barley (쌀보리 약배양을 위한 약치상 방법별 배양효율)

  • Park, Tae-Il;Jeoung, Sun-Ok;Kim, Young-Jin;Kim, Hyun-Soon;Seo, Jae-Hwan;Park, Ki-Hun;Kim, Jung-Gon;Yun, Song-Joong
    • Journal of Plant Biotechnology
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    • v.34 no.3
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    • pp.237-242
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    • 2007
  • Barley anther culture is hard working to plating picking out anther from the glume and demand long time comparing to be short available development stage for effective culture. Also, it has been treatment massive materials due to low plantlet comparing to get desirable plants intensively. Consequently, this experiment was carried out trying to be more high barley anther culture effectively in terms of save plating effort. Plating materials and culture temperature affected anther culture efficiency are among the inoculation tissues or organs such as anthers, spikelets and whole panicles, culture efficiency was higher with spikelets in two-rowed than six-rowed barley due primarily to a lower contamination, and calli were induced within 30 to 50 days. Callus induction and plant regeneration rates were higher in cultures at $25^{\circ}C$ than at $15^{\circ}C$ and $20^{\circ}C$. Days to callus induction were 25 to 50 days at $25^{\circ}C$ and 50 to 60 days at $20^{\circ}C$.