• Title/Summary/Keyword: high acid resistance

Search Result 565, Processing Time 0.026 seconds

Some Probiotic Properties of Some Lactic Acid Bacteria and Yeasts Isolated from Jeot-gal. (젓갈 프로바이오틱 생균의 내산성 및 내담즙 특성)

  • 이나경;김현욱;최신양;백현동
    • Microbiology and Biotechnology Letters
    • /
    • v.31 no.3
    • /
    • pp.297-300
    • /
    • 2003
  • In order to select probiotics with a high survival rate in gut and the growth inhibition of virulent pathogens to human beings or animals, we have screened lactic acid bacteria and yeasts from Jeot-gal to assess resistance against the artificial gastric acid and bile juice. Lactic acid bacteria and yeasts isolated were incubated for 24 h in artificial bile juice after incubation for 2 h in artificial gastric acid. Especially, strain HW 161 and strain NK 181 showed the higher survival for 2 h incubation in artificial gastric acid. All of 3 strains of lactic acid bacteria and 2 strains of yeast were showed higher growth rate than the control in artificial bile. The antimicrobial activity of lactic acid bacteria and yeasts was also investigated to prove efficacy as probiotic organisms. Lactic acid bacteria were shown the inhibition of Gram positive and negative bacteria, but yeasts narrow inhibition.

Probiotic Characterization of Fructobacillus fructosus F2 Isolated from Honeybee Larvae (꿀벌 유충에서 분리된 Fructobacillus fructosus F2의 프로바이오틱스 특성 분석)

  • Woo Young Bang
    • Journal of Life Science
    • /
    • v.34 no.10
    • /
    • pp.691-700
    • /
    • 2024
  • Frutobacillus spp. strains (Fs) were isolated from honeybee larvae to evaluate their functionality and potential use as probiotics. Anti-microbial activity was generally observed in F strains against Gram-negative, Gram-positive, and yeast strains, with F2 and F3 being superior, particularly F2. Kerosene emulsification was similarly observed in strains other, except for F4. Emulsification analysis based on carbon sources showed that F2 had high emulsification in the presence of fructose but lower than the standard strain and 16S rDNA sequence analysis revealed that the F2 was identified as Fructobacillus fructosus. The growth curve of F2 showed maximum growth at 18 hr, followed by a slight increase. Furthermore, antimicrobial activity and pH showed maximum and minimum values at 18 hr, respectively, and remained constant thereafter. Lactic acid content showed a slight decrease after reaching its maximum value at 24 hr of culture. Acid resistance was observed up to pH 2.5, but completely lost at pH 2.0. Bile acid resistance was generally strong. F2's adhesion to mucin was higher compared to S. Typhimurium, which increased until 18 hr of culture and then decreased. Enzyme activity according to anaerobic culture time was high for carboxymethylcellulase (CMCase), avicelase, and mannase, regardless of the presence of fructose. Auto- and co-aggregation were higher compared to the standard strain, and surface hydrophobicity was high for chloroform, which indicates electron donor properties. Therefore, the Fructobacillus fructosus F2 can be considered a potential probiotic due to its excellent anti-microbial, emulsification, acid resistance, bile resistance, CMCase, mannase, and auto- and co-aggregation properties.

Elucidation of Intergranular Corrosion of UNS N08810 alloys (UNS N08810 합금의 입계부식손상과 원인 분석)

  • Kim, Youngsik;Hwangbo, Deok
    • Corrosion Science and Technology
    • /
    • v.11 no.5
    • /
    • pp.196-204
    • /
    • 2012
  • Corrosion failure of petrochemical facilities is one of the difficulties in maintenance, since operating conditions of crude oil production, storage, and refinement are very aggressive. UNS N08810, which has been used for crude oil transportation pipes and storage tanks in petrochemical industries, shows good resistance to general corrosion and localized corrosion in several environments. Among its environments, UNS N08810 showed better corrosion resistance in fuel gas containing sulfuric acid and phosphoric acid and sulfur. However, ductility and toughness at high temperature over about $500^{\circ}C$ were greatly reduced due to microstructural change. In general, welding process is the representative method to join the parts in industrial components. Because the alloy by welding can be sensitized and corroded, the manufacturing process should be controlled. In this work, UNS N08810 was used and heat treatment conditions including solution and stabilization treatments were controlled. Oxalic acid etch test by ASTM A262 Practice A was done to evaluate the qualitative sensitization in room temperature. Huey test by ASTM A262 Practice C was done to evaluate the intergranular corrosion rate in boiling 65% $HNO_3$ solution. Also, the microstructure by thermal history was analyzed. Experimental alloy showed high intergranular corrosion rate and its corrosion mechanism was elucidated.

Review on Changes in Surface Properties and Performance of Polyamide Membranes when Exposed to Acidic Solutions (산성용액 노출 시 폴리아마이드 분리막의 표면성질 및 투과성능 변화에 관한 총설)

  • Lee, Hyung Kae;Dao, Huyen Thi Thanh;Kang, Wooseok;Kwon, Young-Nam
    • Membrane Journal
    • /
    • v.30 no.5
    • /
    • pp.283-292
    • /
    • 2020
  • Various kind of solutions need to be separated, purified, and concentrated using membranes in the field of industries. However, when the solution contains strong acids, the use of membrane is limited. Acid resistant membrane currently available in market does not show high efficiency of flux. This review explains the causes and mechanisms of changes in surface properties and performance of polyamide membranes when exposed to acidic solutions, and this can be used in the development of a membrane with acid resistance and high flux.

Prevalence and Characterization of Plasmid-Mediated Quinolone Resistance Determinants qnr and aac(6')-Ib-cr in Ciprofloxacin-Resistant Escherichia coli Isolates from Commercial Layer in Korea

  • Seo, Kwang Won;Lee, Young Ju
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.8
    • /
    • pp.1180-1183
    • /
    • 2020
  • The prevalence and characterization of plasmid-mediated quinolone resistance (PMQR) determinants in ciprofloxacin-resistant Escherichia coli isolated from a Korean commercial layer farm were studied. A total of 45 ciprofloxacin-resistant E. coli isolates were recovered and all isolates were multidrug-resistant. Eight isolates have the PMQR genes aac(6')-Ib-cr, qnrS1, and qnrB4, and seven isolates exhibited double amino acid exchange at both gyrA and parC, and have high fluoroquinolone minimum inhibitory concentrations. Five transconjugants demonstrated transferability of PMQR and β-lactamase genes and similar antimicrobial resistance. Because PMQR genes in isolates from commercial layer chickens could enter the food supply and directly affect humans, control of ciprofloxacin resistance is needed.

Corrosion resistance improvement of 6061 aluminum alloy using anodizing process

  • Zuraidawani Che Daud;Muhammad Faidzi Shukri;Mohd Nazree Derman
    • Advances in materials Research
    • /
    • v.13 no.3
    • /
    • pp.195-202
    • /
    • 2024
  • Aluminum alloy is a material that is frequently used in the aerospace and transportation industries due to its high mechanical and corrosion resistance qualities. Unfortunately, aluminum alloys are prone to corrosion, limiting their application in some harsh situations such as when submerged in aqueous environments. The purpose of this study is to investigate how anodizing can increase the corrosion resistance of 6061 Aluminum alloy. The anodizing process was carried out using two different parameters which are voltage (5V, 10V, 15V) and electrolyte sulfuric acid (H2SO4) concentration (0.3M, 0.5M) for 1 hour. The anodized samples were performed using several analyses such as X-ray diffraction (XRD) analysis, morphology analysis, and corrosion test. From this study, it is found that the difference in anodizing parameters affects the corrosion resistance of the samples. Sample anodized at 15V, 0.5M gives the best corrosion resistance.

EXPERIMENTAL STUDIES OF THE EFFECT OF EROSION ON THE TOOTH ENAMEL SURFACE BY ACID BEVERAGES PURCHASED ON THE RETAIL MARKET (수종의 시판 청량음료가 치아표면 법랑질 침식에 미치는 영향에 관한 실험적 연구)

  • Ha, Joo-Sik;Kim, Chong-Youl
    • The Journal of the Korean dental association
    • /
    • v.19 no.11 s.150
    • /
    • pp.949-956
    • /
    • 1981
  • The author collected some acid containing beverages to observe the influence which acid containing beverages affect the tooth surface tissue and the resistance of tooth which was treated with fluoride and counterpart tooth in the acid containing beverages and comparatively observing the erosion of enamel surface, the author came to the following conclusion. 1. Acid containing beverages on the market eroded the tooth surface tissue and the erosion degree was determined by the pH concentration of beverages. 2. the erosion aspect of the tooth surface in terms of acid containing beverages indicated some on the surface and surrounding enamel rod in the beverages which the pH was comparatively high, and the erosion was observed greatly in all enamel including the enamel rod in the beverages which pH was high. 4. When one drinks beverages, if beverges' pH is high, there are no serious harmful, but the author recommended not drinking the beverages which pH is low under the situation lack of the proper preventive dentistry technique.

  • PDF

Hard Anodizing Treatment in Malic Acid Bath mixed with Oxalic Acid (말릭산과 수산혼합욕에서 경질양극 산화처리)

  • Jeong, Yong-Soo;Chang, Do-Yon;Kwon, Sik-Chol
    • Journal of Surface Science and Engineering
    • /
    • v.17 no.3
    • /
    • pp.78-86
    • /
    • 1984
  • Hard anodic oxide film was investigated formed on pure aluminium with various temperature (30$^{\circ}-60^{\circ}C$), current densities (1.5-3.0A/$dm^2$) and concentrations(3-15g/l) of oxalic acid in 0.5M malic acid bath. The resulting characteristic of the anodic oxide film obtained were summarized as follows in the view point of physical and mechanical properties in relation with the above process variables. 1. The film thickness increased with oxalic acid concentration and bath temperature, while the reversed phenomena were obtained at a high concentration of oxalic acid and high temperature due to the severe dissolution of the anodic oxide film. 2. The hardness and the abrasion resistance were improved by lowering the addition of oxalic acid and the bath temperature. This feature was directly dependent on the porosity formed on the anodic oxide film. 3. The maximum hardness of anodic oxide film showed Hv 579 in the temperature of 30$^{\circ}C$ with the current density, 2.5A/$dm^2$ in the 0.5M malic acid bath mixed with 5g/l oxalic acid.

  • PDF

Optimal Design of Dual-Structured Disc of a Safety-Valve for the Specialized Pressure Vessel Considering Thermal Expansion (특수 압력요기용 안전밸브의 2중 구조로 디스크의 최적설계)

  • Kim, Chang-Ho
    • Journal of the Korean Society of Manufacturing Process Engineers
    • /
    • v.6 no.4
    • /
    • pp.81-85
    • /
    • 2007
  • A safety valve is used for protecting the pressure vessel and facilities by discharging the operating fluid into the valve from the accident when the pressure is over the designated value. The fluid is sulfurous acid and nitric acid. etc. in the semi-conductor assembly line. Thus the valve elements material must be acid resistance. Teflon, which is used generally as inner parts of a valve, tends to easily sticks to sliding surface by thermal expansion under high temperature. Some studies are performed to change teflon to another material and shape to have a better fluidity under the condition. The analysis of the thermal expansion is conducted by commercial FEM software to improve the problems. Boundary conditions were temperature and load in this study. From the analysis, the thermal expansion of stainless steel is verified to be lower than that of teflon under high temperature. Thus coupled teflon/stainless steel-made valve is applied to assembly line without danger due to thermal expansion.

  • PDF

Drug Resistance and R. Plasmids of Enterobacteria Isolated from Bovine Udder Infections (젖소 유방염유래(乳房炎由來) 장내세균(腸內細菌)의 약제내성(藥劑耐性) 및 R. Plasmids)

  • Park, Cheong Kyu
    • Korean Journal of Veterinary Research
    • /
    • v.21 no.1
    • /
    • pp.25-31
    • /
    • 1981
  • A total of 250 enteric bacteria (148 Escherichia coli, 41 Klebsiella pneumoniae, 46 Enterobacter spp. and 15 Proteus spp.) isolated from bovine udder infections in 1979 through 1980 were examined for drug resistance and prevalence of R. plasmids. The drug tested were streptomycin (SM), kanamycin (KM), ampicillin (AP), chloramphenicol (CP), tetracycline (TC), gentamicin (GM), oxolinic acid (OA) and nalidixic acid (NA). The detection of R. plasmids was performed with Escherichia coli ML 1410 NAr as the recipient. Of the 148 Escherichia coli isolated, 68(45.9%) were found to be resistant to one or more drugs tested, and about 50% of the resistant strains were multiply resistant. of the 68 drugresistant strains, 13(19.1%) were found to carry R. plasmids which were capable of performing a conjugal transfer. CP resistance was transfered together with the other resistance. Of 41 strains of Klebsiella pneumoniae isolated, 90.2% were resistant to the drugs, alone or in combination thereof. Strains resistant to AP and TC were 63.4%, and 48.8%, respectively. R. plasmids were detected in 78.4% of the drug-resistant strains, and these strains transfered all or a part of their drug resistance pattern. AP and CP resistance were transfered in 100% of AP and CP-resistant strains. Eleven (37.9%) of 29 R. plasmids showed a thermosensitive transfer. Of the 46 strains of Enterobacter spp. isolated, 37(80.4%) were resistant to the drugs tested. A high percentage of resistance was noted for AP(65.2%). All strains resistant to four or more drugs transferred their resistances to Escherichia coli ML 1410, but strains resistant to three or fewer drugs did not transfer the resistances. All of the 15 Proteus strains isolated were resistant to more than two drugs. of them, 6 were quadruple resistance to SM, KM, CP and TC, and 9 were double one to AP and TC. Three (20.0%) of the drug-resistant isolates had R.plasmids conferring AP and TC resistance. GM, OA and NA of the drugs tested were very active to all of 250 Gram-negative enteric bacteria isolated from bovine udder infections.

  • PDF