• Textile Coloration and Finishing
• /
• v.15 no.5
• /
• pp.301-309
• /
• 2003

Of the various types of dye that can be used to apply polyamide fibers, acid dyes and pre-metallised acid dyes are great in use so far. However, since these acid dyeings suffer from dye loss during laundering, recourse to an aftertreatment is usually necessary to achieve adequate fastness to washing. In the case of reactive dyes, the characteristically high washfastness of dyeings comes with the concomitant advantages of brightness and low environmental impact. Despite the obvious advantages, the commercialization of reactive dyes for polyamide fibers has not gained widespread success. In this context, the rewards for approach are likely to be considerable. Heterobifunctional reactive dyes were applied to polyamide fibers using various conditions. Optimum conditions and fixation were determined. The forms in which the vinylsulphone and hydrolyzed moieties were examined using HPLC-Mass. In addition, it was found that the extent of the washfastness secured, in terms of both change in color and staining of adjacent multifiber was being acceptable.

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2002.04a
• /
• pp.17-20
• /
• 2002

• Analytical Science and Technology
• /
• v.23 no.6
• /
• pp.537-543
• /
• 2010

Dopamine (DA) is an important neurotransmitter molecule of catecholamines. Its deficiency could lead to brain disorder such as Parkinson's disease and schizophrenia. Therefore, it is necessary to establish a suitable analytical technique with sensitivity and simplicity. A competitive enzyme-linked immunosorbent assay for DA has been optimized and characterized. Assay sensitivity is controlled by two factors in competitive immunoassay. One is a nature and concentration of competitor, and the other is those of binder, antibody. Thus, optimization was performed: BSA-DA conjugate and antibody-avidin conjugate were prepared by dual heterobifunctional coupling method using SATA and SMCC. Assay condition was optimized with $6.66\;{\mu}gmL^{-1}$ of BSA-DA and $4.17{\times}10^{-10}\;M$ of antibody-avidin conjugate. A dose-response curve was constructed, and a limit of detection and a dynamic range for DA were accomplished to $2.3{\times}10^{-2}\;{\mu}g\;mL^{-1}$ and four orders of magnitude ($1.0{\times}10^{-7}\;M$ to $1.0{\times}10^{-3}\;M$), respectively. Calibration curve was constructed on dynamic range and least-squares regression of this data gave the following relationship: absorbance = -0.1098 log[DA]+0.0353 ($R^2$ = 0.9956).

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2004.11a
• /
• pp.190-191
• /
• 2004

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.701-702
• /
• 2000

The thiolated anti-Escherichia coli antibody prepared by thiolation with a thiolcleavable heterobifunctional cross-linker, sulfosuccinimidyl 6[3- (2-pyridyldithio) propionamido]hexanoate (sulfo-LC-SPDP) was chemisorped onto one gold electrode of the piezoelectric quartz crystal surface. In the QCM system employing a batch-type well holder, a steady-state frequency decrease was attained within 20 min when $100{\sim}200\;{\mu}L$ suspensions of Escherichia coli having viable cell counts of $10^5{\sim}10^6\;CFU/mL$ were added. The stability of sensor response was improved compared to the system with a batch-type dip holder.

• 한국신재생에너지학회:학술대회논문집
• /
• 2010.06a
• /
• pp.227.2-227.2
• /
• 2010

본 연구에서는 양극산화된 $TiO_2$ 전극(anodized tubular $TiO_2$ electrode, ATTE)을 수소제조용 PEC(Photoelectrochemical)시스템에서 광어노드와 기존의 백금전극을 대체하고 $H^+$ 환원능을 향상시키기 위하여 엔자임(Pyrococcus furiosus, Pfu)을 고정화한 후 캐소드로 동시에 활용하였으며, 엔자임 고정을 위한 crosslinker 종류 및 금속담지 여부, ATTE 길이를 통한 수소발생양에 미치는 영향을 연구하였다. ATTE 표면과 엔자임의 amine group의 연결을 위하여 heterobifunctional crosslinker로써 사슬 길이가 상대적으로 짧은 Sulfo-SDA가 유리하였으며, 금속담지의 경우 짧은 튜브의 경우 1% 내에서 효과가 증진되었으나 긴 튜브의 경우는 오히려 광전류 및 궁극적으로 수소발생속도에 불리하게 작용하였다. 또한, 튜브 길이가 긴 ATTE가 짧은 ATTE 보다 수소발생양에서 더욱 효율적임을 알 수 있었다. 텅스텐산화물 담지의 가시광감응에의 담지 효과는 예비 실험 결과로 나타나지 않아, 추가적인 연구가 필요한 것으로 판단된다.

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2011.11a
• /
• pp.21-21
• /
• 2011

개인적 삶의 질 향상과 함께 사회적인 삶의 가치를 추구하여 인체친화적이며 동시에 환경 친화적인 제품 소비에 가치를 두는 LOHAS 트렌드의 영향으로 의류 제품에서는 소비자의 요구 수준에 맞춘 고급화, 고기능화된 의류 상품 및 소재 중심의 소비와 함께 친환경 소재, 리사이클 소재 등에 대한 관심이 증가하고 있다. 최근 Ramie 복합 소재는 이러한 트렌드에 적합한 대표적인 소재로서 고감성/웰빙 신섬유 제품 개발을 통해 신시장 개척이 가능한 아이템으로 주목받고 있다. 한편, Ramie는 땀에 대한 흡습성과 속건성이 우수하고 청량감이 있어 주로 여름 소재로 활용되어 왔으나 촉감이 좋지 못하고 광택성이 떨어지는 단점이 있어 이를 보완하기 위해 촉감이 좋고 광택성이 뛰어난 레이온 계열의 소재와 혼방하여 고급스럽고 환경 친화적인 의류소재로써 개발이 진행되고 있다. 본 연구에서는 이러한 레이온계 섬유 중에서 상품성이 modal 섬유 Tencel 섬유를 각각 혼방한 Ramie/Modal(28's,30's), Ramie/Tencel(28's) 복합소재의 반응성 염료에 대한 염색특성을 비교 고찰해 보았다. 실험은 Heterobifunctional type 반응성 염료 3종(Red, Yellow, Blue)을 이용하여 흡진 거동, 빌드업 특성, 겉보기 색상농도 등을 비교하였으며 1/1 standard 농도에서의 제반 견뢰도를 비교 평가하였다.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.14
• /
• pp.5977-5981
• /
• 2015

Background: Worldwide, breast cancer is the most common cancer diagnosed among women and a leading cause of cancer deaths. The age of onset in Iran has become reduced by a decade for unknown reasons. Herceptin, a humanized monoclonal antibody, is a target therapy for breast cancer cells with over expression of HER2-neu receptors, but it is an expensive drug with only 20% beneficial rate of survival. This study introduces a novel approach to enhance the efficacy of this drug through immunoconjugation of the antibody to botulinum toxin. Decreasing the cost and adverse effects of the antibody were secondary goals of this study. Materials and Methods: Botulinum toxin was conjugated with Herceptin using heterobifunctional cross linkers, succinimidyl acetylthiopropionate (SATP) and sulfo-succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (SMCC) according to the supplier's guidelines and tested on two breast cancer cell lines: SK-BR-3 and BT-474. Toxin and Herceptin were also used separately as controls. The cytotoxicity assay was also performed using the new bioconjugate on cultured cells with Alamar blue and a fluorescence plate reader. Results: Herceptin-Toxin bioconjugation significantly improved Herceptin efficacy on both breast cancer cell lines when compared to the control group. Conclusions: Toxin-Herceptin bioconjugation can be a potential candidate with increased efficiency for treating breast cancer patients with over expression of the HER2 receptor.

• Archives of Pharmacal Research
• /
• v.22 no.5
• /
• pp.459-463
• /
• 1999

The effects of the hindered and non-hindered water soluble long-chain disulfide bonds on the stability and cytotoxicity of the ricin A chain (RTA) immunotoxin were examined. The RTA immunotoxins were prepared with the Fab fragments of anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody (Fab-RTA) using sulfosuccinimidyl-6-[(-methyl-(-2-pyridyldithio)toluamido]toluamido]hexanoate (S-LC-SMPT) and sulfosuccinimidyl-6-[3-(2-pyridyldithio-propionamido]hexanoate (S-LC-SPDP). The prepared Fab-RTA immunotoxins were evaluated for their conjugation yield, immunoreactivity, thermal and disulfide bond stability and cytotoxicity. The conjugation yield of the Fab-RTA immunotoxin from the water soluble long chain crosslinking agents, S-LC-SMPT and S-LC-SPDP, were comparable. Both Fab-RTA immunotoxins exhibited a similar immunoreactivity and thermal stability in aqueous solution. However, S-LC-SMPT -mediated Fab-RTA, sterically hindered, showed an enhanced disulfide bond stability in vitro over S-LC-SPDP mediated one. In the cytotoxicity against antigenic cell Daudi, the S-LC-SMPT -mediated RTA immunotoxin maintained a comparable cytotoxicity, compared with S-LC-SPDP mediated Fab-RTA immunotoxin.

• Macromolecular Research
• /
• v.13 no.4
• /
• pp.293-296
• /
• 2005

Transferrin ($T_{f}$) has been used as a targeting ligand for delivering liposome/interleukin-12 (IL-12) pDNA complexes to cancer cells mostly due to the greater number of transferrin receptors ($T_{f}R$) found on tumor cells than on normal cells. $T_{f}$ was conjugated to liposomes via the reaction of MPB-PE with thiol groups of $T_{f}$ introduced by a heterobifunctional cross-linking agent, N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP). Four days after C26 inoculation when the tumor volume reached ${\sim}100mm^{3}$, tumor-bearing Balb/c mice were injected intravenously with $T_{f}-liposome/IL-12 pDNA$complexes twice a week for 3 weeks. Significant suppression of tumor growth was achieved in the group treated with the $T_{f}-liposome/IL-12 pDNA$ complexes, with a dose of $10{\mu}g$ of IL-12 pDNA showing the highest suppression effect among the tested doses. Similar results were obtained when the therapy was initiated one day after tumor inoculation, although in this case $30{\mu}g$ IL-12 pDNA/$T_{f}-liposome$ complexes showed a significant suppression of tumor growth between 19 and 23 days after tumor inoculation. This result indicates that the transferrin receptor-targeted liposomal system is an efficient delivery agent of therapeutic genes, such as IL-12, in mice and that its potential clinical use warrants further research investigation.