• Asian Journal of Turfgrass Science
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• v.18 no.4
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• pp.211-219
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• 2004

Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $\beta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1\%-0.3\%$.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.1-8
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• 2008

The widespread and diverse sulfonylurea (SU) resistance problem has found in Korea, where one-shot-treatment herbicides such as pylazosulfuron/molinate and bensulfuron/molinate have been used continuously since 1989. The SU-resistant weeds of 7 annual weeds and 3 perennial weeds as of 2008 have confirmed in paddy fields in Korea. An effective management to SU-resistant weeds requires an integrated approach toward the weed control system, in particular, as to the drastic changes of herbicides development. Recent trend of new paddy herbicides in Japan has been developing to maximize the management of SU-resistant weeds. In the future, it is expected that the development of paddy herbicides in Korea is likely to be shifted toward the new "one-shot-treatment" included with herbicides of over 3-ways to maximize the control of resistant weeds. Bromobutide and carfentrazone are effective against sedges and broad-leaved weeds, respectively, and benzobicyclone and pyrimisulfam are effective against sedges and broad-leaved weeds.

• Weed & Turfgrass Science
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• v.6 no.1
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• pp.32-39
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• 2017

This study was carried out to investigate the ACCase inhibiting herbicide resistant Echinochloa oryzicola collected in a Honam rice field and to investigate the control of E. oryzicola in directly seeded paddy rice field. The study result showed that about 44% of the 67 biotypes collected in the rice field in Honam region were resistant to ACCase-inhibiting herbicides. The efficacy of several chemical herbicides on these resistant E. oryzicola biotypes was evaluated according to their application timings. Among herbicides applied 4 days before seeding, pretilachlor, butachlor, oxadiazon, oxadiargyl, oxadiazon+pretilachlor, and fentrazamide+oxadiargyl, provided 95% or higher control of the resistant E. oryzicola biotypes. Among the herbicides applied 10 days after seeding, bromobutide+fentrazamide+imazosulfuron, benzobicyclon+fentrazamide+imazosulfuron, pyrazosulfuron-ethyl+thiobencarb, fentrazamide+imazosulfuron, bromobutide+imazosulfuron+mefenacet and bromobutide+imazosulfuron+pyraclonil provided ${\geq}95%$ control of the weeds. Bromobutide+imazosulfuron+mefenacet and bromobutide+pyrazosulfuron-ethyl+pyriminobac-methyl, applied 15 days after seeding, provided ${\geq}95$ and 90% control of the weeds. Foliar application of cyhalofop-butyl+propanil at 30 days after seeding provided ${\geq}90%$ control of the weeds. In addition, oxaziclomefone provided ${\geq}90%$ control for 40 days after soil application. These results show that E. oryzicola biotypes resistant to ACCase-inhibiting herbicides could be effectively controlled in directly seeded rice in submerged paddy fields using single or tank-mixed chemical herbicides currently available on the market.

• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.28-33
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• 2004

This study was conducted to simple transformants selection by herbicide Basta treatment after transformation with Agrobacteium tumefaciens MP90/PAT in hybrid poplar(Populus alba ${\times}$ P. glandulosa No. 3). In preliminary study, we determined that the lethal concentration of herbicide Basta was 1.0mg/L in callus culture, adventitious bud formation and axillary bud elongation experiment. By the treatment of 1.0mg/L Basta, we could be selected the transformed shoots effectively from the various cultures. In addition, the treatment was useful on selection of transformants which are growing in soil pot. Finally, we also confirmed the transformation by PAT assay, Above results show that the herbicide Basta treatment and PAT assay can be a very simple and effective method for the identification of PAT gene transformed hybrid poplar.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.50-56
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• 2012

The objectives of this research were to quantify resistance levels of transgenic rice expressing the bar gene to glutamine synthetase (GS)-inhibiting, and methionine sulfoximine and photosynthesis-inhibiting herbicide, paraquat, and compare the ammonium accumulation, chilling injury, and yield between transgenic and non-transgenic rice. The transgenic rice lines were 45-96-fold more resistant to glufosinate-ammonium than non-transgenic rice. The transgenic rice lines were also 18-fold more resistant to methionine sulfoximine, but was not resistant to paraquat, which has different target site. Glufosinate-ammonium increased the ammonium accumulation in leaves of non-transgenic rice plants, but had minimal or no effect on leaves of transgenic lines. The transgenic lines except for 258, 411, 607 and 608 were more susceptible during chilling and recovery than non-transgenic rice plants. The yield of transgenic lines 142, 144, 258 and 608 was similar or higher than that of non-transgenic rice in pot conditions.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.98-98
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.288-289
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.424-424
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.84-85
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• 2004

• Korean journal of applied entomology
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• v.56 no.4
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• pp.331-338
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• 2017

In the present study, we investigated the effects of two herbicide-resistant genetically modified rice (GM rice) varieties, Milyang 204 and Iksan 483, recently developed in Korea on non-target insects and a spider. No difference in host preferences of the English grain aphid Sitobion avenae and the brown planthopper Nilaparvata lugens were observed between GM rice and non-GM rice. Wolf spider Pirata subpiraticus, feeding on N. lugens reared on GM rice or non-GM rice, revealed no significant difference in body weight. P. subpiraticus, fed with N. lugens reared on Milyang 204, showed survival rates similar to that in P. subpiraticus fed with N. lugens reared on non-GM rice. However, P. subpiraticus feeding on N. lugens reared on Iksan 483 demonstrated significantly lower survival rates than that in P. subpiraticus feeding on N. lugens reared on Milyang 204 or non-GM rice. In addition, when larvae of the western honeybee Apis mellifera were supplied with Iksan 483 pollen, a significantly longer pupal period occurred, as compared with that of A. mellifera supplied with pollen of Milyang 204 or non-GM rice. As GM rice has negative effects on P. subpiraticus, which is an important predator in agricultural ecosystems, and on A. mellifera, which plays important roles in pollination and honey production, additional studies on risk assessment of GM rice should be conducted before releasing newly developed herbicide-resistant GM rice to the agricultural environment.