• 생약학회지
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• 제26권3호
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• pp.265-272
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• 1995

Inhibitory effects of 130 medicinal plants on hyaluronidase activity were analyzed. The medicinal plants are clinically used as herbal medicines for korean traditional prescriptions. Six out of the 130 herbal medicines exhibited more than 50% of inhibition on hyaluronidase activity by their total methanol extracts with 5mg/ml as a final concentration. The active total methanol extracts were prepared from cortex of Acantbopanax gracilistylus, lignum of Caesalpinia sappan, radix of Glycyrrhiza uralensis, radicis cortex of Morus alba, herba of Prunella vulgaris, and radix of Sanguisorba officinalis. These active total methanol extracts were sequentially fractionated with dichloromethane, ethyl acetate, n-butanol, and then water. Among the solvent-fractionated extracts, the butanol fractions of Acantbopanax gracilistylus and Glycyrrhiza uralensis with 1 mg/ml as the final concentration exhibited more than 50% of inhibition on hyaluronidase activity, and the other fractions with the same concentration did less than 20% of inhibition.

• 한국버섯학회지
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• 제17권1호
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• pp.34-39
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• 2019

한국과 중국에서 재배한 약용버섯인 노루궁뎅이, 동충하초, 영지의 열수추출물과 70% ethanol 추출물에 대해서 DPPH 라디컬 소거능, 아질산 소거능, 총 폴리페놀 함량, 베타글루칸 함량을 비교하기 위하여 측정하였다. DPPH 라디컬 소거능은 열수추출물과 70% ethanol추출물에서 한국산 노루궁뎅이와 동충하초가 월등히 높았으며, 추출용매별로 비교하였을 경우, 노루궁뎅이와 동충하초는 열수추출물의 항산화효능이 높았다. 아질산염 소거능의 경우, 한국산과 중국산 영지의 열수추출물에서 각각 41%와 39%로 나타나 70% ethanol 추출물에 비하여 높았다. 총 폴리페놀 함량은 한국산 노루궁뎅이와 동충하초는 열수추출물에서 높은 함량을 보였으며, 영지의 경우는 70% ethanol 추출물에서 더 높은 함량치를 나타냈다. 베타글루칸 함량은 한국산 동충하초가 45.11%를 보이며 가장 높았으며, 영지 역시 중국에서 재배한 것에 비하여 높은 함량을 나타냈다. 노루궁뎅이는 중국산이 한국산에 비하여 높은 베타글루칸 함량을 보였다. 위와 같은 결과로부터 각각의 버섯류에 맞는 최적의 용매를 사용해야 최대의 유효성분을 추출할 수 있다고 사료된다. 본 연구에서 사용한 노루궁뎅이, 동충하초, 영지가 한국 및 중국에서 재배되는 버섯의 대표성을 나타내기에는 부족하나 이러한 연구결과를 토대로 더 많은 비교샘플을 확보하여 약용버섯류에 대한 기능성 평가를 위한 기초자료로 활용하고자 한다.

• 대한본초학회지
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• 제38권3호
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• pp.1-10
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• 2023

Objective : Obesity in modern society has a significant impact on pathological, psychological and social problems. Therefore, many studies on obesity treatment using herbal extracts with fewer side effects have been conducted. This study was designed to investigate the effect of inhibiting fat accumulation in vitro in order to Mongolian medicinal plants find anti-obesity candidate among. Methods : We measured pancreatic lipase inhibitory activity in Mongolian medicinal plants extract. Cytotoxicity of these extracts was monitored in 3T3-L1 cells by MTT assay. In addition, the anti-obesity effects was confirmed at concentrations of 0.2, 0.1 and 0.05 mg/㎖ through Oil red O staining. Results : Among Mongolian medicinal plants, Rheum undulatum roots in September (RURS), Paeonia anomala L. (PAL), and Fragaria orientalis (FO) showed the highest pancreatic lipase inhibitory activity. As a result of the MTT assay, more than 80% was judged to be non-toxic, and the concentration was determined, and as a result of evaluating the lipid accumulation inhibitory effect, 6 types were selected as candidates. Conclusion : Based on these results, the top 7 species expected to be used as anti-obesity functional materials were selected. However, additional efficacy verification and mechanism of action need to be established in the future. So, it is expected that the medicinal plants verified through this will be used as functional materials for the prevention and treatment of obesity.

• 한국버섯학회지
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• 제10권4호
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• pp.249-253
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• 2012

밀리타리스 동충하초 추출물은 체내 염증반응을 유도하는 peroxinitrite를 밀리타리스 동충하초 추출물 $128{\mu}g/ml$ 처리로 peroxinitrite를 $60.77{\pm}1.67%$ 저해시켜 뛰어난 저해능을 가지는 것으로 free radical의 생성을 억제하며, Raw 264.7 세포에 LPS $1{\mu}g/ml$을 처리하여 NO 생성이 $3.75{\pm}0.20{\mu}M$까지 증가하였으나 밀리타리스 동충하초 추출물 $32{\mu}g/ml$ 처리군에서 $3.21{\pm}0.10{\mu}M$, $64{\mu}g/ml$ 처리군에서 $3.07{\pm}0.13{\mu}M$로 농도 의존적, 유의적으로 감소 시켰다. 또한, 이로 인해 발현되는 염증 유전자 COX-2의 발현을 유의적으로 저해 한 것을 Western blot으로 확인 하였다. 그러므로, 밀리타리스 동충하초 추출물은 항염증소재로의 가능성이 충분하다고 사료된다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.151-152
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• 2009

• 동아시아식생활학회지
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• 제19권6호
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• pp.1049-1053
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• 2009

본 연구는 두충을 주 원료로 고안된 한방 혼합 다류가 고혈압 질환자의 혈압, 혈청지질 및 혈액학적 변화에 미치는 영향을 평가하기 위하여 수행하였다. 한방 혼합 다류를 시험 4주간 복용한 그룹과 시험 전 그룹을 비교한 결과, 수축기 혈압은 167.14 mmHg에서 134.86 mmHg로, 이완기 혈압은 100.00 mmHg에서 87.10 mmHg로 뚜렷하게 저하되었다. 혈청지질 중 총콜레스테롤(TC), 고밀도-콜레스테롤(HDL-C), 저밀도-콜레스테롤(LDL-C)의 농도 및 동맥경화지수(AI)는 유의적인 차이가 없었다. 반면, 한방 혼합 다류를 복용 시 중성지방(TG) 농도가 237.10 mg/dL에서 145.00 mg/dL로 강하게 감소되었다. 시험 4주 후와 시험개시 전의 혈액학적 지표(적혈구, 백혈구, 헤마토크리트, 헤모글로빈, 혈소판 및 호모시스테인)를 비교한 경우, 유의적 변화는 관찰되지 않는 반면 평균 적혈구 혈색소 농도(MCHC)는 유의적으로 2.70% 증가되었다. 이상의 결과로 부터 두충을 주 원료로 고안된 한방 혼합 다류는 중성지방 농도를 감소시킴으로써 혈압을 저하시키는 것으로 추정하며, 평균 적혈 수혈 색소 농도를 증가시킴을 확인하였다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.272-273
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• 2011

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
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• pp.219-220
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• 2012

• 한국약용작물학회지
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• 제24권5호
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• pp.375-385
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• 2016

Background: The study was conducted to elucidate the extraction conditions under which white ginseng has cognition-improving efficacy. Methods and Results: Extracts from white ginseng under different solvent and temperature conditions were analyzed for ginsenoside content and inhibitory effect on N-methyl-D-aspartate (NMDA) receptor and acetylcholinesterase. The total ginsenoside contents and amounts of ginsenoside Rb1 plus ginsenoside Rg1 from the 1st extracts (prepared with EtOH/$H_2O$ as solvent) were higher than those from the 2nd extracts (extracted with $H_2O$ after the 1st EtOH/$H_2O$ extraction). The contents in the 1st and 2nd extracts produced at $80^{\circ}C$ were also higher than those obtained at $50^{\circ}C$. Samples from the 1st extraction at $80^{\circ}C$ indicated higher inhibitory activities on NMDA receptors-whose excessive activation is thought to mediate the calcium-dependent neurotoxicity associated with several neurodegenerative diseases-than those from the 2nd extraction. Among the samples prepared at varying temperatures, the extract prepared at $50^{\circ}C$ showed the highest suppression activity on NMDA receptors. Note, however, that the extracts from the 2nd extraction at $50^{\circ}C$ inhibited acetylcholinesterase-whose inhibition could be a therapeutic strategy for neurodegenerative diseases with cognitive deficits and memory malfunction-more effectively than those from the 1st extraction. Conclusions: To enhance the cognition-improving activity of white ginseng extract, it is suggested that the extracts be utilized after being combined the 1st extracts (made with EtOH/$H_2O$ solvent) and the 2nd extracts (prepared with $H_2O$) at low temperature.

• 한국약용작물학회지
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• 제18권1호
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• pp.28-33
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• 2010

Glycyrrhiza uralensis (Leguminosae) is a well-known herbal medicine that has long been valued as a demulcent to relieve inflammatory disorders. To compare the influence of different solvents on the anti-inflammatory efficacy of G. uralensis, we measured the inhibition of pro-inflammatory mediators such as NO, TNF-$\alpha$, and $PGE_2$ in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells by extracts produced using different solvents (water, methanol, ethanol, or n-hexane). The results showed that methanol was the most effective solvent for the inhibition of both NO and $PGE_2$ production in RAW 264.7 cells. However, there was no difference among the extracts for inhibition of TNF-$\alpha$. Further study must be performed for the analysis of correlation between the anti-inflammatory activity of extracts produced using different solvents and the content of major bioactive compounds in G. uralensis, such as glycyrrhizin and liquiritin. The present study suggests that methanol may be a more appropriate solvent of G. uralensis than other solvents (water, ethanol, and n-hexane) to yield the greatest anti-inflammatory activity for food additives and medicine.