• The Korea Journal of Herbology
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• v.27 no.5
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• pp.65-75
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• 2012

Objectives : Cosmeceutical activities such as anti-oxidative and anti-aging effects of the Doinseunguitang and its composition, a traditional prescription, were evaluated. Methods : We performed MTT assay, melanin synthesis inhibition assay, DPPH free radical scavenging assay, SOD-like activity, xanthine oxidase inhibition assay, astringent activity assay, tyrosinase inhibition assay, elastase and collagenase inhibition assay. Results : The results were obtained as follows : DPPH free radical scavenging of water extract Doinseunguitang (DISG) and ethanol extract DISG was 60% and 50% at 1,000 ppm. Xanthine oxidase inhibition effect of ethanol extract showed more than 80% at 500 ppm. Tyrosinase inhibition and inhibition melanin synthesis activities were measured in 40% and 50% at 1,000 ppm and 100 ppm. Elastase and collagenase inhibition rate of ethanol extracts DISG 40% and 80% at 1,000 ppm. It was concluded that compositive ingredients (Persicae Seman, Glycyrrhizae Radix, Cinnamomi Ramulus, Rhei Rhizoma) influenced the most results. Conclusions : The results indicated that, ethanol extract which is superior in its anti-oxidative and anti-aging effects is useful to be applied in herbal cosmetic industry.

• Korean Journal of Korean Medical Institute of Dermatology and Aesthetics
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• v.1 no.1
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• pp.98-113
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• 2005

This paper provides comparison and analysis between brand images of Korean herbal cosmetics and general, imported cosmetic brands, focusing on product's symbolic and functional images. The objective is to evaluate brand images of Korean herbal cosmetics to suggest a powerful brand image strategy that will be different and competitive. Data collection was performed on 300 adult women who were over 20 years of age and lived in Seoul or KyongKi area. Analysis was carried out using SPSS version 11.5 and frequency analysis, T-Test and $X^2$ verification was also performed. Analysis results for brand images of Korean herbal cosmetics are as follows. First, satisfaction scale as a purchase feature for users of Korean herbal cosmetics were 'Normal' 51.5%, 'Satisfactory' 35.1%, 'Very Satisfactory' 8.2% and 'Unsatisfactory' 5.2%. Regarding improvements, 'Efficacy, Effect' was the highest with 46%. Second, 'SEOLHWASOO' and 'BAEKOKSENG' among Korean herbal cosmetic brands, 'IOPE' and 'ISA KNOX' among general cosmetic brands and 'LANCOME' and 'CHANEL' among imported cosmetic brands displayed high recognition. Third, the symbolic and functional images of Korean herbal cosmetic brands were seen to be better compared to their general and imported counterparts. Especially, product related features of Korean herbal cosmetic brands received a more positive response compared to imported brands, which suggests that with research and improvement regarding non-product related features, functional images of herbal brands may be appraised ahead of imported brands. Fourth, there is a significant difference in brand preference according to the satisfaction of groups who actually use herbal cosmetics and the purchasing attitudes of groups who do not. Therefore, an active relationship marketing strategy is required to maximize satisfaction and to elicit favorable attitudes. By developing functional native ingredients, constructing data that can prove its efficacy and effects, and appealing its unique, differentiated symbolic value together with Orientalism, herbal medicines will be able to take off to be acknowledged worldwide.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.4 s.59
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• pp.283-298
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• 2006

The domestic market of oriental herbal cosmetics has rapidly grown in the last few years. In this study, we investigated female consumers' cosmetics-buying behavior to know their appreciation of oriental herbal cosmetics. We also tried to deduce the strategies for growth and development of the oriental herbal cosmetics market in the future. A survey was conducted with women ranging in age from 20 to 50, who resided in Seoul; a total of 400 questionnaires were distributed and then 344 copies of those. were collected and analyzed statistically. This study provides basic information about the present status of oriental herbal cosmetics market and the real state of the cosmetic consumption. The consumers needs identified in this study, provides basic data in setting a marketing strategy for the oriental herbal cosmetics market.

• The Korea Journal of Herbology
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• v.24 no.4
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• pp.101-106
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• 2009

Objectives : We investigated the anti-aging effects on skin with 9 Repetitive steaming and fermenting herbal composition extract (FHE). Methods : Herbal composition is comprised of Panax Ginseng, Rehmannia glutinosa, Polygonum multiflorum and Polygonatum Sibiricum. They steamed and fermented with Saccharomyces cerevisiae and Lactobacillus brevis by turns at 9 times. We measured various effects related to skin aging such as scavenging activity against free radical, cell proliferation, collagen synthesis and toxicity of 9 repetitive steaming and fermenting herbal composition extract were evaluated and compared with herbal composition extract (HE). Results : As the number of steaming and fermenting cycle increased free-radical scavenging activity were increased. But cell proliferating ratio was not increased when the number of steaming and fermenting cycle. The FHE could significantly increase the collagen synthetic ratio compared with HE treated group. And, FHE was showed no toxicity at all tested concentrations. Conclusions : The results of our study propose that FHE has good anti-aging effects on skin.

• Journal of Haehwa Medicine
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• v.22 no.1
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• pp.79-90
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• 2013

Objectives : With increasing interests in hair cosmetic to keep their scalp and hair healthy we must have much more information about the oriental medical cosmetic goods. Methods : Shampoos which contain herbal ingredients are commonly used to stopping their hair loss, alopecia. On the basis of oriental pharmacology, we analyzed several shampoos which have herbal ingredients and thought about the oriental cosmetic methods for a hair loss. Results : We found that many herb based shampoos are developed on the basis of the principle of oriental medicine. In addition, the interest in oriental medical cosmetic methods that used the principles of the oriental medical science has been increased and cosmetic goods based on the oriental medicine have a positive market. Conclusions : With many possibilities for the oriental medical cosmetic methods, clinical records to prove scientific facts are urgently needed. In addition, the oriental medical cosmetic methods based on oriental medicine to examine scalp and hair should be offered.

• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.262-267
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• 2013

The purpose of this study was to research for anti-oxidation and anti-wrinkle effects of Acar mono Sap (AM). To cosmetic effect of AM, safety effect (MTT assay), anti-wrinkle effect (elastase, MMP-1 inhibition assay) and anti-oxidant effect (DPPH assay) were measured. When water extract of AM was used for cell viability, it was over 100% at 6% (6 ml/100 ml in phosphate buffer) concentration. AM showed 45.7% elastase inhibition and 23.7% MMP-1 inhibition at 50% (50 ml/100 ml in phosphate buffer) concentration so that it had good anti-wrinkle characteristic. And AM showed 68.9% antioxidation capacity at 50% concentration by using a DPPH assay. Consequently, AM can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the anti-wrinkle effect, for cosmetic compositions.

• The Korea Journal of Herbology
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• v.30 no.4
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• pp.95-100
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• 2015

Objectives : The purpose of this study was to research the whitening effects and developing by cosmetics of the extract fromDioscoreae Rhizoma, which is one of the most popular health-promoting herb in herbal medications.Methods : We performed tyrosinase inhibition assay, reverse transcription-polymerase chain reaction (RT-PCR) and western blot for whitening effects. Also we measured MTT assay for cell viability.Results : The results were obtained as follows : For whitening effect, tyrosinase inhibition rate of extract fromDioscoreae Rhizomashowed more than 42.28% at 1,000 ㎍/㎖ concentration. Cell toxicity effect on melanoma cells (B16F10) of extract fromDioscoreae Rhizomashowed 81.97% with toxicity at 50 ㎍/㎖ concentration. So we were measured at a concentrations of 5, 10 and 50 ㎍/㎖ in all experiments involving cell. In addition, whitening related mRNAs including microphthalmia associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), tyrosinase were reduced byDioscoreae Rhizoma. We also foundDioscoreae Rhizomatransiently decreased protein kinase A (PKA) which is known to be upstream to the down regulation of MITF and tyrosinase. But phosphorylation of extracellular signal related kinase (pERK) were increased byDioscoreae Rhizoma. These results imply thatDioscoreae Rhizomadecrease melanogenesis via ERK activation and subsequent down regulation of MITF and tyrosinase.Conclusions : Therefore, all these findings suggested the potent usage ofDioscoreae Rhizomaas materials of functional cosmetics by confirming whitening activity related with melanin content.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.157-163
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• 2014

Objectives : Gamisoyo-san complex prescription were made with Angelicae Gigantis Radix, Paeoniae Radix, Atractylodes rhizome white, Hoelen, Bupleuri Radix, Moutan Cortex Radicis, Gardeniae Fructus, Zingiberis Rhizoma Crudus, Menthae Herba. The purpose of this study was to research the whitening effect of the extract from Gamisoyo-san, which is one of the used herbal complex prescription. Methods : This study investigated inhibitory effect of Gamisoyo-san in tyrosinase activity. Cell viability were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Then, Gamisoyo-san measured reversed-transcription-PCR for mRNA expression using B16F10 mouse melanoma cells. Results : For whitening effects, the tyrosinase inhibition effect of extract was shown to 52.4% at $5,000{\mu}g/m{\ell}$ concentration. The cell viability on B16F10 melanoma cells of Gamisoyo-san extract showed higher than 75% at $1,000{\mu}g/m{\ell}$ concentration. In this study, an experiment was performed by setting the non-toxic concentration range of 50, 150, $250{\mu}g/m{\ell}$. The Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a positive control. The microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), tyrosinase mRNA expression inhibitory by reverse transcription-PCR of Gamisoyo-san extract were decreased by 95.3%, 98.8%, 96.3% and 49.5% at $250{\mu}g/m{\ell}$ which the highest concentration. Conclusions : All these findings could verify that whitening effects of Gamisoyo-san extract by tyrosinase inhibitory activity and mRNA expression. The Gamisoyo-san could be used as material for functional cosmetics, such as skin whitening products.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.6
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• pp.380-384
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• 2017

From March 1, 2017 to May 31, 2017, subjects were allowed to apply a wrinkle-improving herbal cosmetic product containing the extract of Douchi for 8 weeks. The effectiveness of herbal cosmetics was evaluated through wrinkle improvement efficacy parameter analysis, subjective wrinkle improvement through questionnaires, and adverse reaction. The herbal cosmetic products containing the extract of Douchi showed a significant decrease in the wrinkle improvement efficacy parameter analysis result. The results of the subjective satisfaction analysis after use were also evaluated positively. We could confirm the possibility of wrinkle improvement effect of Douchi extract, and it will be possible to contribute to the expansion of korean medicine field through more studies in the future.

• Archives of Pharmacal Research
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• v.19 no.2
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• pp.122-125
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• 1996

We examined the effect of topical application of Shimotsu-to, a traditional Chinese herbal prescription, on carrageenin-induced edema in rats and ultraviolet radiation-induced erythema in guinea pigs. Shimotsu-to (5% in water) markedly suppressed an acute edema of rat hindpaw induced by 1% carrageenin, and was more effective than any other single crude drug componcent of Shimotsu-to, Topical treatment with this prescription also inhibited ultraviolet erythema on the back skin of guinea pigs (a human sunbrun model). These results suggest the therapeutic effect on acute inflammation by topical application of Shimotsu-to.