• 대한수의학회지
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• 제43권3호
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• pp.323-331
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• 2003

We performed this study to determine the late biological effect of gamma radiation and effect of Bu-Zhong-Yi-Qi-Tang (BZYQT), a prescription of traditional Oriental medicine, on radiation-induced late biological effect (survival, hematological change, carcinogenesis) of mice irradiated with 3 Gy of gamma-radiation. There were little difference in body weights between normal and irradiated mice. Survival rate were decreased in irradiated mice and the survival rate and mean survival time of the groups treated with BZYQT were far better than the irradiation control group. A significant elevation of total leukocyte or lymphocyte counts was seen at week 4 and 12 of the group treated with BZYQT. Stimulated recovery by the extract from BZYQT was also observed in thrombocyte. Main gross findings of irradiated mice were appeared as enlargement of spleen, thymus and liver, tumorous nodules of lung and cyst or mass of ovary. Microscopically, there were various findings including hematopoietic and lymphoid tumor, lung cancer, ovarian cancer and cancer of other lesions. BZYQT reduced the incidence of tumor development. Further studies are needed to characterize better the protective nature of ingredients and active compounds.

• 한국환경과학회지
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• 제16권5호
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• pp.665-670
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• 2007

본 연구에서는 실험동물을 이용하여, $NO_2$의 반복적으로 폭로($0.025mg/m^3$, 8h/day /4 weeks)하여, 실험군 및 대조군에서의 혈액학 및 생화학적 요인들의 변화와 상관성을 평가하였 였으며, 그 결과를 요약하면 다음과 같다. $NO_2$의 흡입 폭로로 인한 혈액학적 요인의 변화는 백혈구의 경우 동물실험에서의 폭로군에서 통계적으로 유의하게 증가하였다(p<0.05). hematocrit의 경우 폭로군에서 통계적으로 유의하게 감소하는 경향을 나타내었다. 또한 hemoglobin과 hematocrit는 전혈 중에서 적혈구의 비율을 나타내는 것이므로 3가지 종류 모두 유사한 경향을 나타내었다. methemoglobin의 경우 동물실험에서 폭로군에서 유의하게 증가하였다(p<0.05). 생화학적 요인에 대한 $NO_2$의 영향은 동물실험에서 total protein과 triglyceride이 동물 실험에서의 폭로군에서 감소하였으나 통계적으로 유의하지는 않았다. albumin이 폭로군에서 감소하였고, blood urea nitrogen과 creatinine의 경우 폭로군에서 높은 경향으로 나타났다. uric acid 및 혈청 중 lactate dehydrogenase의 경우 폭로군이 대조군 보다 높은 농도를 나타내어 $NO_2$의 폭로에 의해 호흡기 조직세포 내 이상을 일으킨 것으로 판단된다. 따라서 실내오염물질 중 산화가스의 하나인 $NO_2$에 대한 위해성 평가는 저농도에서의 폭로연구가 장기적이고 지속적으로 이루어져야 할 것으로 사료된다.

• 사상체질의학회지
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• 제17권3호
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• pp.91-102
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• 2005

1. Objectives The purpose of this study is to find out effects of Hyeongbangpaedok-san(HBPDS) and Dokhwaljihwang(DHJH) against decline of physical function as aging. 2. Methods Administrating HBPDS and DHJH to 40-week-old Wistar rat for 10 weeks so, I researched weight change, weight change of internal organs, and hematological and serological changes. 3. Results & Conclusions 1. Both examining groups, which were taken HBPDS and DHJH, got more weight than control group. But that was regardless. 2. Both examining groups got more weight on internal organs than control group. But that was regardless, too. 3. Both examining groups decreased in amount of MDA in serum, as contrasted with control group. But it was regardless. 4. Both examining groups improved on hematological condition. WBC, RBC, Hgb, monocytes and eosinophil rates were decreasing and HCT and PLT were increasing. Especially monocytes(p<0.001) and eosinophil(p<0.05) rate of DHJH taken group was decreased remarkably. 5-1. Both examining groups show decline in each item of functional examination of liver, such as ALT, AST, T-bilirubin, T-protein, ALB, A/G. T-chol, TG, etc. HBPDS taken group showed meaningful decline in Albumin(p<0.01) and A/G(p<0.01) and DHJH taken group showed meaningful decline in T-bilirubin(p<0.01). 5-2. Both examining groups showed decline in items of functional examination of kidneys. Specially HBPDS taken group showed meaningful decline in CRN rate(p<0.05) and DHJH taken group showed meaningful decline in BUN rate(p<0.05). As those results, HBPDS and DHJH are effective against decline of physical function as aging.

• Safety and Health at Work
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• 제2권3호
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• pp.290-300
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• 2011

Objectives: There is limited data regarding the toxicity of methylcyclohexane, despite its wide use in rubber adhesives, paint diluents, and cleansing agents. This study aimed to verify the toxicity and influence on the reproductive system of methylcyclohexane after its repeated injection in Sprague Dawley (SD) rats. Methods: Methylcyclohexane was injected subcutaneously into male and female SD rats once a day, five times a week, for 13 weeks at different doses (0, 10, 100, and 1,000 mg/kg/day) for each group. The toxicity of testing material was verified by observing the change in body and organ weight, hematological change, pathological findings, and effect on the reproductive system at each different concentration. Results: In the 1,000 mg/kg/day group, there were cases of animal deaths. In animals that survived, hematological changes, including a decrease in the red blood cell count, were observed. A considerable weight gain or loss and pathological abnormalities in the liver, kidney, and other organs were found. However, the 10 and 100 mg/kg/day groups did not cause deaths or other specific abnormalities. In terms of reproductive toxicity, there were changes in hormone levels, including a significant decrease in hormones such as estradiol and progesterone (p < 0.001) in male animals. Menstrual cycle change for female animals did not show concentration dependency. Conclusion: When injected repeatedly for 13 weeks, methylcyclohexane proved to be toxic for the liver, heart, and kidney at a high dose. The absolute toxic dose was 1,000 mg/kg/day, while the no observed adverse effect level was less than 100 mg/kg/day. The substance exerted little influence on the reproductive system.

• 혜화의학회지
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• 제20권1호
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• pp.91-104
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• 2011

Background : Although chronic non-bacterial prostatitis is increasing, it is hard to treat effectively. In western medicine, antimicrobials drug, ${\alpha}$-adreno-ceptor antagonists, anti-inflammatory drugs, tricyclic antidepressants and anticholinergic agents are used commonly, but chronic prostatitis/chronic pelvic pain syndromes is confusing and frustrating for urologist. IDS(Indongsoyeom-bang) is used in treatment of chronic prostatitis/chronic pelvic pain syndromes. And it is reported that GLS(Gleditsiae spina) and TOF(Toosendan fructus) components of IDS have significant effect on protection of the glandular epithelial cells. Objective : In this study was conducted to investigate the therapeutic effects and action machanism of IDS in the rat model of non-bacterial prostatitis induced by castration and testosterone treatment. Methods : We observed six experimental objects of normal group, control group, testosterone group, and IDS 50 mg/kg, 200mg/kg, 400mg/kg group. Rats were treated with 17 ${\beta}$-estradiol after castration for induction of experimental non-bacterial prostatitis, which is similar to human chronic prostatitis in histophatological profiles. IDS and testosterone were administered as an experimental specimen and a positive control, respectively. The prostates were evaluated by histological parameters including the epithelial score and epithelio-stromal ratio for glandular damage. Also, the prostates were observed by Hematological alterations of WBC, RBC, hemoglobin and platelet. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with IDS-50 showed a diminished range of the tissue damage. Epithelial score was improved in IDS than that of the control. The epithelio-stromal ratio was lower in IDS when compared to that of the control. Also, the examination of bloods were not observed hematological change. Conclusion : These finding suggests that IDS may protects the glandular epithelial cells. We concluded that IDS could be a useful remedy agent for treating chronic non-bacterial prostatitis.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.181-191
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• 2017

A comparison of the growth, hematological values, fatty acids, and gonadal and growth hormonal changes of river puffer, Takifugu obscurus, tiger puffer, T. rubripes, their hybrids (river puffer ${\times}$ tiger puffer) and hybrid triploids was performed during 3 months of their early growth period. Several features were observed during these 3 months: hybrids showed the highest levels of specific growth rate, 1.48%; hybrid triploids showed the smallest change in viscera fat (P<0.05), but GSI was not significantly different among groups (P>0.05). Considering hematological parameters, hybrid triploids had increased mean corpuscular volume and mean corpuscular hemoglobin (P<0.05), but other parameters were not significantly different between groups (P>0.05). With respect to fatty acids, puffer fish, hybrids and hybrid triploids contained fatty acids such as SFAs, MUFAs, n-3 PUFAs and n-6 PUFAs. There were significantly different amounts of total fatty acids between groups (P<0.05), however, rates of changes in fatty acids did not differ significantly between groups (P>0.05). Gonadal hormone (estradiol and testosterone) changes in the river puffer and tiger puffer were significantly higher than that observed in hybrids and hybrid triploids. The hybrids and tiger puffers had higher amounts of growth hormone (thyroid stimulating hormone and thyroxine) than the hybrid triploids and river puffers (P<0.05).

• 사상체질의학회지
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• 제9권2호
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• pp.203-225
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• 1997

Jihwangbakhotang(地黃白虎楊) is made by Li Je Ma, the creator of the Four Constitutional Medicine. Single and 13 weeks oral repeated dose toxicity studies were conducted in Sprague Dawley rats of both sexes to elucidate the potential acute and subchronic toxicity of JBT extract and reversibility of any effects. In the single dose study, JBT extract was administered orally to rats with the dose of 2 g/kg and 8 g/kg. In the long term administration of 13 weeks, the JBT extract of 125 mg/kg/day, 500 mg/kg/day, 2000 mg/kg/day was administered to rats. The change of blood weight, urine volume, electrolyte in urine, hematological change, the change of blood chemistry, autopsy finding, and histological observation were researched, the results were as follows; 1. The lethal dose of JBT extract seems to be over 10 g/kg, the single administration of JBT extract 8 g/kg showed no toxical signs except little increase of urine volume. 2. The change of body weight had the trend of decrease in the group of, but has no significance, and also the consumption of food and water had no changes. 3. The hematological changes induced by the 13 weeks administration of JBT extract showed the significance in the item of Hb, MCH, MCV, WBC in the group of 125 mg/kg/day. 4. In the test of blood chemistry, total cholesterol showed little decrease and A/G ratio showed little increase, but the change was not clear, and the standard error was large. So the result was obtained insignificantly and the toxicity of JBT extract was not observed. 5. In the male group after recovery period, the level of cholesterol and triglyceride decreased slightly, but the result was not significant. 6. In the urine test, the little change of electrolyte was appeared, but it seemed not to be the result induced by the toxicity of JBT extract. 7. In each group of male and female rats, the weight change of organ and the serum histological changes was observed, but the result did not showed the dose dependent toxicity. So the toxicity of JBT extract was not regarded. In the conclusion, the toxicity of JBT extract was not observed in the single dose treatment and long term repetitive administration of JBT extract.

• Molecules and Cells
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• 제38권6호
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• pp.482-495
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• 2015

Sphingolipids such as ceramide, sphingosine-1-phosphate and sphingomyelin have been emerging as bioactive lipids since ceramide was reported to play a role in human leukemia HL-60 cell differentiation and death. Recently, it is well-known that ceramide acts as an inducer of cell death, that sphingomyelin works as a regulator for microdomain function of the cell membrane, and that sphingosine-1-phosphate plays a role in cell survival/proliferation. The lipids are metabolized by the specific enzymes, and each metabolite could be again returned to the original form by the reverse action of the different enzyme or after a long journey of many metabolizing/synthesizing pathways. In addition, the metabolites may serve as reciprocal biomodulators like the rheostat between ceramide and sphingosine-1-phosphate. Therefore, the change of lipid amount in the cells, the subcellular localization and the downstream signal in a specific subcellular organelle should be clarified to understand the pathobiological significance of sphingolipids when extracellular stimulation induces a diverse of cell functions such as cell death, proliferation and migration. In this review, we focus on how sphingolipids and their metabolizing enzymes cooperatively exert their function in proliferation, migration, autophagy and death of hematopoetic cells, and discuss the way developing a novel therapeutic device through the regulation of sphingolipids for effectively inhibiting cell proliferation and inducing cell death in hematological malignancies such as leukemia, malignant lymphoma and multiple myeloma.

• 대한본초학회지
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• 제22권4호
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• pp.35-43
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• 2007

Objective : The aim was to study experimentally the effects of Daeyoungjeon(DYJ) on the hypothyroidism of rats induced by thiouracil. Methods : Spargue-Dawley rats were separated into four groups. Except normal group, the other three groups were treated with thiouracil 0.50g/kg/days for 30 days by oral administration. Among the three groups, one group was treated DYJ extract 0.75g/kg/days for 20 days separately. Another group was treated DYJ extract 1.50g/kg/days for 20 days separately. T3-uptake, T3, T4, TSH, hematological values, enzyme activity, total cholesterol, triglyceride and Na+ content change in the Serum of rats were measured after medication of solid extract of DYJ. Results : As a result of this study, in the serum T3-Uptake and T4 level, hematological values and sodium were increased in DYJ group compared to control group. The serum TSH, total cholesterol, triglyceride, enzyme activity and body weight were decreased in DYJ group compared to control group. Conclusion : According to the above results, it is suggested that DYJ is effective to cure hypothyroidism.

• 한국수산과학회지
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• 제44권5호
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• pp.484-489
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• 2011

This study examined whether N-acetylcysteine (NAC), a glutathione precursor, could attenuate toxic effects of three fish anti-parasitic agents, trichlorfon, hydrogen peroxide ($H_2O_2$) and formalin, all of which are known to exert side effects through free radical production. Common carp Cyprinus carpio were fed with NAC (approx. 50 mg/kg/day) for 3 consecutive days prior to anti-parasite bathing for a 24 hr period. Mortality rates were examined during this 24 hr bathing period, and selected hematological and biochemical parameters were also assessed at the termination of anti-parasite exposure. The mortality rates and plasma glucose elevations caused by all three anti-parasitics were significantly reduced by NAC pretreatment. Trichlorfon, but not $H_2O_2$ or formalin, elevated plasma levels of aspartatetransaminase (AST) and alanine-transaminase (ALT), and these elevations were attenuated by NAC. There was no change in hematocrit values in any treatment. The results provide evidence for the attenuating effects of NAC against toxicity caused by anti-parasite agents that act through free radical-producing properties. The results found in this study also suggest that NAC may be administered to fish to minimize toxicity in fish parasiticide use.