• The Korean Journal of Physiology
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• v.7 no.2
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• pp.49-58
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• 1973

The distensibility of the major arteries has been investigated extensively, but the value expressed as Young Modulus varies widely by the different schools of the investigators, the major reason undoubtedly being the difficulties encountered in the measurement. In the present study, an attempt was made to elucidate the distensibility of the external carotid artery of the rabbit, which was placed in saline immediately after removing from the apparently healthy, normal rabbit without anesthesia. The circular section strip and longitudinal section strip were made from the whole artery, and Young Modulus of the whole artery, circular section and longitudinal section strips was calculated from the length-tension curve of each sample. Also, the similar samples of the artery seperately obtained were placed in ATP solution in the concentration of 0.15 mM and 0.30 mM, and Young Modulus was similary calculated. Experiments were performed at 15,45 and 75 min after the artery was removed from the rabbit, and the results thus obtained are summarized as follows. 1) Young Modulus of the whole external carotid artery of the rabbit in saline was $4.74{\times}10^7dyne/cm^2$ at 15 min, but lower values were obtained at 45 and 75 min, Young Modulus being $4.62{\times}10^7dyne/cm^2\;and\;4.13{\times}10^7dyne/cm^2$, respectively. When the arterial samples were placed in ATP solutions, Young Modulus did not change much throughout the experiment, and lower Young Moduli were obtained in 0.30 mM ATP solution than in 0. 15 mM ATP solution. 2) Young Modulus Of the Circular Section Strip in Saline was $4.11{\times}10^7dyne/Cm^2,\;3.75{\times}10^7dyne/cm^2\;and\;3.90{\times}10^7dyne/cm^2,$ respectively, at 15, 45 and 75 min, the value at 15 min being the highest. However, when the strip was placed in ATP solutions, no appreciable change was observed throughout the experiment, and Young Moduli were lower in 0.30 mM ATP solution than in 0.15 mM ATP solution. 3) Young Modulus of the longitudinal section strip in saline was $2.12{\times}10^7dyne/cm^2,\;2.48{\times}10^7dyne/cm^2\;and\;2.46{\times}10^7dyne/cm^2$, respectively, at 15, 45 and 75 min, Young Modulus being slightly elevated in the latter part of the experiment. A similar tendency was observed when the strip was placed in ATP solutions.

• Korean Journal of Psychosomatic Medicine
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• v.18 no.1
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• pp.19-29
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• 2010

Objectives：Authors hypothesized that bipolar tendency of non-psychiatric person would be associated with stress vulnerability. To test the hypothesis, we compared perceived stress level, Type A Behavioral Pattern (TABP) and unhealthy lifestyle between person with and without bipolar tendency. Methods：The study cohort consisted of 1987 subjects without past and current psychiatric history. In this study, bipolar tendency was determined by MDQ response which requires endorsement of at least 7 of the 13 "yes or no" questions. We compared the scores of Perceived Stress Scale, A/B lifestyle questionnaire, and unhealthy lifestyle(alcohol, smoking, lack of exercise, irregular meal) between MDQ positive and negative respondents. Results：We identified the bipolar tendency group determined by the MDQ positive response were 329 subjects(16.6%). The overall score of the bipolar tendency group was significantly high on the A/B lifestyle questionnaire compared to the non-bipolar tendency group(125.4 vs. 115.9, p<0.001), and the rate of the subjects defined as TABP was also significantly high(41.3% vs. 30.1%, p<0.001). In comparison of subjectively perceived stress level, the bipolar tendency group was observed to be significantly higher than the non-bipolar tendency group (18.5% vs. 16.5%, p<0.001). The bipolar tendency group was significantly higher than the non-bipolar tendency group on the rate of answering：'irregular eating habit' (20.1% vs. 14.3%, p=0.002), '4-or-more-times alcohol intake per week' (29.8% vs. 16.5%, p<0.001), 'currently smoking' (41.9% vs. 23.0%, p<0.001), '2-or-less-times physical exercises per week' (63.2% vs. 55.1%, p=0.007). Conclusion：By clinical diagnosis, even a person who does not meet the criteria of bipolar disorder has a high risk of stress-related behavioral pattern, and perceived stress when possessing bipolar tendencies such as the (hypo) maniac tendency or affective instability. Such individual has significantly higher risk of having unhealthy life-style such as smoking, drinking, irregular eating habit and lack of exercise compared to non-bipolar tendency group. Since these risk factors lead to increase the risk of obesity, metabolic syndrome, diabetes, and cardiovascular diseases, the individual with the bipolar tendency requires a more effort to manage stress and to maintain healthy lifestyle.

• Applied Microscopy
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• v.39 no.3
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• pp.205-218
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• 2009

This experiment was performed to evaluate the morphological responses of the gastric epithelial cells of the mouse, inoculated with Ehrlich carcinoma cells in the inguinal area, following administration of BCG. Healthy adult ICR mice weighing 25 gm each were divided into normal and experimental groups (tumor control group and BCG-treated group). In the experimental groups, each mouse was inoculated with $1{\times}10^7$ Ehrlich carcinoma cells subcutaneously in the inguinal area. From next day after inoculations, 0.2 mL of saline or BCG (0.5 mL/25 g B.W.: $0.03{\times}10^8{\sim}0.32{\times}10^8$ CFU) were injected subcutaneously to the animals every other day, respectively. The day following the 7th injection of saline or BCG, each mouse was injected with a single dose of 0.7 ${\mu}Ci/g$ of methyl-$^3H$-thymidine (25 Ci/mmol, Amersham Lab., England) through tail vein. Seventy minutes after the thymidine injection, animals were sacrificed, and gastric tissues were taken and fixed in 10% neutral formalin. Deparaffinized sections were coated with autoradiographic emulsion EM-1 (Amersham Lab., England) in a dark room. The number of labeled epithelial cells in the gastric mucosae (mean number of labeled epithelial cells per 3.5 mm length of mucosa) were observed and calculated. And for electron microscopic observation, gastric tissues were prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide solution. On the light microscopic study, gastric mucosae had no morphological changes following the injection of BCG. On the electron microscopic study, in the BCG-treated mice, myelin figures and multivesicular bodies within the gastric epithelial cells were observed more frequently than in those of the normal control ones. On the autoradiographic study, number of the labeled cells of normal control, tumor control and BCG-treated mice were 380.2 (${\pm}31.35$), 426.1 (${\pm}28.43$) and 301.8 (${\pm}34.63$), respectively. In the BCG-treated mice, poorly-labeled cells containing only a few silver grains of 3H-thymidine were observed more frequently as compared in those of the normal control and tumor control ones. From the above results, BCG may suppress the DNA synthesis of the gastric epithelial cells, but does not results severe fine structural defect on the gastric epithelial cells. These results suggest that BCG is expected as one of the effective supplemental anticancer drugs.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.2
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• pp.6-14
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• 1980

One of the physiological disease, sudden wiliting of Yushin variety suggested that low sunlight, excessive nitrogen application, and highly reduced soil condions either singly or combined, might be possible causes of the disorder. Some visual symptom of sudden wilting are discoloration of leaves, development of nodal roots above the soil surface, total root rot, and lodging. Those observations led to the hypothesis that suffocation of root tissues was a direct cause of the wilting. The oxygen transport characteristics of Yushin, IR262 and Tongil were examined by two methods. First, Soil-cultured plants of the three varieties were subjected to paraffin treatment to decrease the oxygen supply from the air to root tissues through the soil-water system, liquid paraffin was applied to the water surface in the pots at panicle formation stage. In this experiment, sudden wilting was observed of Yushin and IR262 at about a week after the treatment, but Tongil remained green and healthy. Wilting-resistant variety Tongil had higher oxygen release, whereas the susceptible Yushin and IR262 had lower oxygen release. Second, the number and size of the air spaces in each internode were investigated in the 5th internode from the top, all three varieties have a similar number of air spaces, although the air spaces of Thongil were larger. In the 4th internode, Tongil had plenty air spaces, Yushin and one of the Yushin's parents IR262 had scanty or none. The observations indicated that the ability of Yushin and IR262 for oxygen transport is very limited compared with that of Tongil. The limited oxygen supply due to poor development of air space in internode of rice plants may cause suffocation of root tissues, weaken metabolic activity of the tissues, and induce root rot, subsequently inducing sudden wilting and lodging under unfavorable weather, soil and cultural conditions.

• The Korean Journal of Pesticide Science
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• v.10 no.4
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• pp.313-319
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• 2006

Two hundred bacterial strains were isolated from the soil around healthy tomato plants in a polyvinyl house, where most of the other plants showed bacterial wilt symptoms. The strains were screened in vitro for their antibacterial activity. Among them, a strain, KPB3 showed strong bactericidal activity against bacterial wilt pathogen, Ralstonia solanacearum. The strain KPB3 was identified using physiological and biochemical tests, and 16S rRNA analyses. Based on these tests, the strain was found to be closer to genus Paenibacillus. To control the bacterial wilt caused by R. solanacearum, greenhouse experiments were conducted to determine the effectiveness of the Paenibacillus strain KPB3. Drench application of this strain ($4{\times}10^8$ CFU $mL^{-1}$) into the pots containing tomato plants, post-inoculated with the pathogen, R. solanacearum could drastically reduce the disease severity, compared to the non-treated plants. To evaluate effectiveness of this strain under field conditions, experiments were carried out in polyvinyl houses infested with R. solanacearum, during spring and autumn of the year 2006. It was observed that, during spring, bacterial wilt was more prevalent compared to the autumn. During spring, 50.9% disease incidences occurred in non-treated controls, while, Paenibacillus strain KPB3 treated plants showed 24.6% disease incidences. Similarly, during autumn, around 17.2% plants were infected with bacterial wilt in non- treated polyvinyl houses, compared to the Paenibacillus strain KPB3 treated plants, which showed 7.0% disease incidences. These results demonstrated that, Paenibacillus strain KPB3 is a potential biological control agent against bacterial wilt caused by R. solanacearum, effective under greenhouse as well as field conditions. This is the first report showing biocontrol of R. solanacearum using a Paenibacillus spp. under field conditions.

• Journal of Nutrition and Health
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• v.50 no.1
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• pp.85-97
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• 2017

Purpose: This study was conducted to investigate dietary habits related to weight reduction and snack intake habits of $4^{th}$ to $5^{th}$ grade elementary students located in Jeju-si, Jeju, South Korea. Methods: The $4^{th}$ and $5^{th}$ grade elementary school students (total n = 234, equally matched numbers of normal weight children and overweight/obese children (n = 117/group)) were surveyed using a questionnaire and a 24-hour dietary recall method in Oct 2015. Results: The percentage of students who experienced reduced food intake to control weight was 26.3% in the normal weight group (NG) and 77.6% in the obese group (OG). Most frequently answered meal for reduced intake was snacks in the NG and dinner in the OG. Percentages of daily calorie intake among subjects were 17.2% for breakfast, 33.8% for lunch, 29.7% for dinner, and 19.3% for snacks. Frequency of snack intake was 2.1 times a week in NG and 1.6 times a week in OG, which showed a statistical difference between body weight groups. Types of snack foods were distinctly different depending on where children consumed them, although no difference was observed between NG and OG. In addition, snack intake level of low energy and nutrient-dense foods was significantly lower in the OG compared to the NG, whereas snack intake level of energy dense and low-nutritive foods was not different between the OG and NG. Conclusion: Taken together, snack intake level with consideration of frequency and amount of snack intake showed that children in the OG consumed significantly less low energy and nutrient-dense foods compared to the NG. Therefore, nutritional education for choosing healthy snack foods for children regardless of body weight status is crucial based on family-school links.

• Journal of Chest Surgery
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• v.42 no.6
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• pp.738-743
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• 2009

Background: There is no standardized tool and parameter that can accurately assess the sympathetic function before and after performing sympathectomy in patients with primary palmar hyperhidrosis. We examined the effective-ness of the sympathetic skin response (SSR) study for documenting the change of sympathetic denervation before and after performing selective thoracic sympathicotomy. Material and Method: We prospectively investigated the SSR from 12 healthy subjects who were diagnosed with primary hyperhidrosis. Each SSR was recorded on the right palm or sole with electrical stimuli applied to the skin at the left wrist and foot and vice versa for the controlateral side. This test was performed before, 2 weeks and 1 year after selective thoracic sympathicotomy. The data was corrected for the onset latency and the amplitude of the SSR (n=24). Result: The mean age of the 12 patients was $24.6{\pm}0.4$ years (range: 19~36) and the gender ratio was 1 : 0.7. The mean values of the preoperative, postoperative 2 weeks and postoperative 1 year onset latency and amplitude of the palmar side (n=24) were $1.46{\pm}0.24$ msec and $6,043{\pm}2,339{\mu}V$, $1.63{\pm}0.42$ msec and $823{\pm}638{\mu}V$, and $1.44{\pm}0.39$ msec and $2,412{\pm}1,546{\mu}V$, respectively. The mean values of the plantar side (n=38) were $1.83{\pm}0.42$ msec and $2,816{\pm}1,694{\mu}V$, $2.16{\pm}0.39$ msec and $1,445{\pm}1,281{\mu}V$ and $1.95{\pm}0.25$ msec and $1,622{\pm}865{\mu}V$, respectively. Among the documented parameters, only the palmar amplitude (p=0.002) showed statistical significance in recording the change of the sympathetic system within the same individual for the pre and postoperative period. Conclusion: The SSR amplitude ratio may be a useful parameter for documenting the efficacy of sympathetic denervation after selective sympathicotomy.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.2
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• pp.115-127
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• 1973

Leaf discoloration of IR667 lines (tropical) and leading locals (temperate) in fields was classified according to the probable causes and nutritional disorder due to soil reduction in 1972 was investigated. 1. The causes of leaf discoloration in IR667 were low air temperature, soil reduction, seed born, insect bite, nitrogen depression, overdose pesticide, strong wind, early senescence and unknown one. 2. Leaf discoloration due to soil reduction which has been called Sageumbyeong by famers, was caused by the heavy application of $Ca(OH)_2$, compost and poor drainage followed by Zn and K deficiency and Fe toxicity. 3. About 30 days after transplanting deficiency concentration of K and Zn in leaf blade appears to be less than 2.0% and 20ppm respectively, and greater than 200ppm, 500ppm, and 1.0% respectively for toxicity or excess of Fe, Mn and Ca. and in the shoot 2.4% for K, 30ppm for Zn and 800ppm for Fe. The value of K/Ca should be greaterthan 2.0 for health. 4. When plants were damaged by soil reduction the contents of N, P, Ca, Mg, Fe, Mn, Na in shoot were increased and those of K, Zn, Si were decreased. 5. IR667 lines show in shoot higher content of N, P, Ca, Mg, Si, Na, and lower content K, Zn, Fe, Mn and lower root activity than local leading varietles in either healthy or disieased case, indicating IR667 lines are likely more suseptible to soil reduction damage. 6. Normal soil was less than 6.5 of pH and greater than -50 mv of Eh, but pH of problem soil was ranged from 6.7 to 7.4 and Eh from -100 to -190. 7. The root activity (${\alpha}$-naphthylamine oxidation) decreased at early stage of soil redudtion damage, then increased with severity and at the end it decreased again, but IR667 lines showed always lower root activity than local ones.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.1
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• pp.52-60
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• 2000

This study was conducted to gain basic data for alleviation of gas emission and conservation of healthy soil environment by investigating an aspect of gas emission and microbial diversity due to the supplement of different fertilizers after application with a livestock manure compost in greenhouse soils. Green pepper was cultivated in clay loamy soil from April to August. Before planting, a livestock manure compost was applied with $741mg\;ha^{-1}$ on the basis of the phosphate content contained in compost. And then, deficient nitrogen for cropping was supplemented with either quick-acting fertilizer of urea or a controlled slow release fertilizer made from urea formaldehyde(U/F). $NH_3$ and R $NH_2$ gases emitted from soil showed a low concentration in the early stage but a maximum in 27 days after planting, then decreased rapidly and not detected after 33 days. Their average concentrations were 42% and 85% lower in the treatment of slow release fertilizer than that of urea fertilizer, respectively. $CO_2$ gas emitted under urea fertilization was ranged from 1,200 to $3,200mg{\ell}^{-1}$ and that in slow release fertilizer was $900{\sim}2,650mg\;{\ell}^{-1}$. The average concentration of urea treatment was $2,260mg{\ell}^{-1}$ and 30% higher than that of slow release fertilizer. The treatment of slow release fertilizer with the lapse of cropping time populated larger in numbers of bacteria, actinomycetes, nitrate bacteria and nitrate reduction bacteria, and ratios of bacteria and actinmycetes to fungi than that of urea fertilizer. But the number of fungi was higher in the treatment of urea fertilizer and denitrifying bacteria showed a similar trend in both treatments. The microbial diversity index, which calculated with numbers of 6 species of microorganisms, was decreased with increasing of growing stage in the range of 0.1 to 0.35 and that was higher in the tratment of slow release fertilizer than urea.

• Journal of Veterinary Clinics
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• v.32 no.4
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• pp.308-312
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• 2015

This study was aimed to evaluate the effects of tramadol hydrochloride on the minimum alveolar concentration of isoflurane ($MAC_{ISO}$) in dogs. Six healthy, female German shepherd dogs (aged 1-2 years) were used in this study. Anesthesia was induced by mask induction and maintained with isoflurane in oxygen. Mechanical ventilation maintained the end-tidal $CO_2$ partial pressure ($P_{ET}CO_2$) from 35 to 45 mmHg throughout the study. A baseline $MAC_{ISO}$ ($MAC_{ISO}B$) was determined starting 45 minutes after induction of anesthesia by clamping a pedal digit until gross purposeful movement was detected. After $MAC_{ISO}B$ determination, dogs received a tramadol loading dose of 3 mg/kg followed by a continuous rate infusion (CRI) of 2.6 mg/kg/h. The determination of $MAC_{ISO}$ after administration of tramadol ($MAC_{ISO}T$) began 20 min after the start of the CRI. Arterial blood pressure and heart rate were recorded continuously and arterial blood samples for blood gas analysis were collected at the end of the equilibration period. Mean ${\pm}$ SD values for the $MAC_{ISO}B$ and $MAC_{ISO}T$ were $1.33{\pm}0.04%$ and $1.23{\pm}0.04%$, respectively. The $MAC_{ISO}B$ decreased significantly by $7.5{\pm}0.2%$ (P < 0.05) after administration of tramadol. The mean heart rate and arterial blood pressure of six dogs were not changed significantly after tramadol administration. The blood gas levels remained constant during the study. In conclusion, tramadol could significantly reduce $MAC_{ISO}$ without depression of cardiorespiratory function. Thus, the use of tramadol on inhalation anesthesia with isoflurane in dogs can improve the stability of anesthesia and the quality of recovery.