• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.05a
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• pp.588-591
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• 2018

Baculovirus was originally isolated from the alfalfa looper and contains a 134-kbp genome with 154 open reading frames (ORF). The major capsid protein VP39 together with some minor proteins forms the nucleocapsid ($21nm{\times}260nm$) that encloses the DNA with p6.9 protein. They are double-stranded, circular, supercoiled DNA molecules in a rod-shaped capsid. Wild-type baculoviruses exhibit both lytic and occluded life cycles that develop independently throughout the three phases of virus replication. Recombinant baculoviruses can transfer their vectors and express their recombinant proteins in a wide range of mammalian cell types. Especially, inclusion of a dominant selectable marker in these baculoviral vectors can express diverse recombinant genes in many cells. Baculoviral vectors were reconstructed with cytomegalovirus (CMV) promoter,uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These reconstructed vectors were infected into various cell and cell lines. We performed transfection and expression of these recombinant vectors comparison with other control vectors. From this study, we knew that transfection and expression of these recombinant vectors have higher efficacy than any control vector. This work was supported by a grant from Mid-Career Researcher Program(NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.11
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• pp.691-700
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• 2016

This study was performed to provide data for developing a more effective and practical pilates exercise program for young women. To meet this purpose, 20 young women were assigned to both groups. The experiment was conducted according to the experimental procedure. Ten subjects in the pilates exercise group performed exercise for 60 minutes everyday at a frequency of 3 days per week for 8 weeks. The subjects in both groups were measured for their physical composition, isokinetic muscular strength in legs, and body balance before and after the experiment. After 8 weeks, the pilates exercise group's body composition showed significantly increased muscular amount (p <.01). There was a statistical increase in knee joint muscle function. For the right knee joint and left knee joint, right knee joint extensor muscle's maximum muscular strength and total work significantly increased under conditions of $60^{\circ}/sec$ (p<.05). Under conditions of $180^{\circ}/sec$, there were statistically significant increases in left knee joint flexor muscle's maximum muscular strength (p<.05). For balance, balance of both legs and right leg showed statistically increases (p<.05).

• Journal of dental hygiene science
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• v.14 no.4
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• pp.442-447
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• 2014

The aim of this study was to analyze perception of risk and using status of hand-held dental x-ray unit among dental hygienists in metropolitan area. This research was based on perception of risk and using status of hand-held dental x-ray unit survey in 257 dental hygienists from July 7 to 31, 2014. Data were analyzed with t-test, one way ANOVA, and multivariable logistic regression analysis using SPSS Windows 21.0 program and significance level was set at p<0.05. The hand-held dental x-ray unit to cover all type patients was 24.8%. The 62.6% of patients and 81.3% of operator doesn't wear lead apron, the experience rate of radiation education was 40.1%. The average of risk perception was 3.08 points. The associated factors of risk perception were career, number of radiation exposure per day, and possession of fixed x-ray unit. The increased of accumulated dose when the occupationally exposed work continues, it is necessary to effort of defense against dental radiation.

• The Journal of Internal Korean Medicine
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• v.25 no.3
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• pp.461-472
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• 2004

Objectives : For the screening of neuroprotective effects of medicinal herbs, the complex system of animal models suffer some disadvantages in controlling critical parameters such as blood pressure and body temperature. Additionally, application of drugs to the appropriate brain area sometimes is difficult, due to poor permeability though the blood brain barrier, and so potential protective effects might be masked. Methods : Organotypic hippocampal slice culture (OHSC) method has the advantages of being relatively easy to prepare and of maintaining the general structure, including tissue integrity and the connections between cells. Drugs can easily be applied and neuronal damage can easily be quantified by using tissues and culture media. This study demonstrates neuroprotective effects of Puerariae radix (葛根, PR), Salviae miltiorrhizae radix (丹蔘, SR), Rhei rhizoma (大黃, RR), and Bupleuri radix (柴胡, BR). These were screenedand compared to MK-801, antagonist of NMDA receptors, by using OHSC of 1 week-old Sprague-Dawley rats. Oxygen/glucose deprivation (OGD) were conducted in an anaerobic chamber $(85%\;N_2,\;10%\;CO_2\;and\;5%\;H_2)$ in a deoxygenated glucose-free medium for 60 minutes. Water extracts of each herbs were treated to culture media with $5\;{\mu}g/ml$ for 48 hours. Results : Neuronal cell death in the cultures was monitored by densitometric measurements of the cellular uptake of propidium iodide (PI). PI fluorescence images were obtained at 48 hours after the OGD and medicinal herb treatment. Also TUNEL-positive cells in the CAI and DG regions and LDH concentrations in culture media were measured at 48 hours after the OGD. According to measured data, MK-801, PR, SR and BR demonstrated significant neuroprotective effect against excessive neuronal cell death and apoptosis induced by the OGD insult. Especially, PR revealed similar neuroprotective effect to MK-801 and RR demonstrated weak neuroprotective effect. Conclusions : These results suggest that OHSC can be a suitable method for screening of neuroprotective effects of medicinal herbs. (This work was supported by the research program of Dongguk University and Grant 01-PJ9-PG1-01CO03-0003 from Ministry of Health & Welfare.)

• The Journal of Korean Physical Therapy
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• v.13 no.3
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• pp.585-596
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• 2001

The twenty one individuals with total knee replacement who were admitted to Kang-Dong Hospital for comprehensive physical therapy were studied in order to demonstrate the effectiveness of an isokinetic test program using the Cybex 6000 machine. The subjects were divided into three groups with the isometric exercise group receiving isometric exercise, the isokinetic eccentric exercise group, and isokinetic concentric exercise group receivind isokinetic exercise(eccentric, concentric) to knee flexors and extensors muscles for a six weeks' period using the Cybex 6000. The results are follow: 1. The extensors were increased significantly at all groups after 6 weeks training(p<.05). The flexors were increased significantly at isokinetic eccentric and isokinetic concentric group but no significantly differences at isometric group(p>.05) 2. At the effect of extensors and flexors after 6 weeks training. higher to 30$^{\circ}$ /sec of isokinetic concentric exercise, lower to 120$^{\circ}$ /sec of isometric exercise. 3. The peak torque was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 4. The total work was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 5. The ratio of peak torque to body weight were more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 6. The average power was more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 7. The average R.O.M in the pre-exercise and post-exercise was not different significantly in all three groups. According to the above results, In the muscle strength recovery for total knee replacement patients, isokinetic concentric exercise group was significantly greater than the isokinetic eccentric and isometric exercise groups after a six weeks training.

• Journal of Distribution Science
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• v.16 no.7
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• pp.77-86
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• 2018

Purpose - In the insurance distribution channel, the salespeople plays a role of representing the company, and recognizes the needs of the customers and plays a role in responding to them. Therefore, their turnover can have a great influence on the company performance. The purpose of this study is to investigate the structural relationship between salespeople's personal - environmental fit and organizational commitment and turnover intention. Research design, data, and methodology - Data collection was conducted a self-filling questionnaire for salespeople for about one month from July 24, 2017 to August 30, 2017. The subjects of the questionnaire were the insurance salespeople who work in the sales line such as K life, S fire. A total of 450 copies were distributed and 432 copies of the questionnaire were used for final analysis. The analysis program used SPSS 22.0 and AMOS 22.0 programs. Analysis method was Frequency Analysis, Reliability & Confirmatory Factor Analysis, Correlation Analysis and Structural Equation Model(SEM). Results - As a result of Hypothesis 1, Person-Supervisor Fit had significant influence only on Continuance Commitment. Person-Job Fit did not have a significant effect on Organizational Commitment. Person-Coworker Fit had a significant effect on Continuance Commitment and Affective Commitment. Person-Organization Fit had a significant impact on Affective Commitment. Therefore, only , , , , were adopted. As a result of Hypothesis 2, Continuance Commitment had a significant effect on turnover intention. Therefore, only was adopted. Conclusions - This study suggests that it is necessary to manage the human resources in the sales field through studies related to salespeople's extension of the research scope and salespeople's turnover intention. Based on the results of this study, the conclusion suggests some implications for the efficient human resources management of insurance companies in sales channels. It is expected that it will be helpful for the salespeople to find out what kind of Person-Environment Fit affects the organizational commitment and how to manage the organizational commitment among the three dimensions of organizational commitment to reduce turnover intention.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.2
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• pp.601-609
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• 2016

This study evaluated the dynamic balance and pain after 12 weeks aquatic exercise in old osteoarthritis patients. The dynamic balances were assessed using 2 different experimental conditions (Open Eye Condition; OEC, Closed Eye Condition; CEC). Twenty-two subjects were assigned one of the two experimental groups: arthritis group (ART, age=$65.25{\pm}5.9$, n=11), control group(CON, age=$64.50{\pm}7$, n=11). All subjects participated in the aquatic exercise program three times a week for 12 weeks. The dynamic balances (OEC (p<.001), CEC (p<.05)) capability were improved significantly in the ART group after 12 week aquatic exercise. Visual Analogue Scale (VAS) was decreased significantly [Factor 10(Soft chair, p<0.01), 11 (Lying down, p<0.05), 12(Handicap, p<0.05), 13 (Work interference, p<0.01)] in the ART group. In conclusion, aquatic exercise was effective in improving the overall health status and the capacity of dynamic balances and reducing the degree of pain in osteoarthritis patients.

• Journal of Internet Computing and Services
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• v.17 no.2
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• pp.59-65
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• 2016

The result of analyzing the cognitive reaction due to the color environment has been applied to various filed especially in medical field. Moreover, the study about the identification of patient's condition and examination the brain activity by collecting the bio-signal based on the color environment is being actively conducted. Even though, there were a variety of experiments by convention the color environment using a light or LED color, it still has a problem that affects the psychological information. Therefore, our proposed system using a HMD (Head Mounting display) to provide a completed color environment condition. This system uses the BMS(Biomedical System) to collect the biometric information which responds to the specific color condition and the human body response information can be measured by the development the Memory and Attention test on Mobile phone. The collection of Biometric information includes electro cardiogram(ECG), respiration, oxygen saturation (Sp02), Bio-impedance, blood pressure will store in the database. In addition, we can verify the result of the human body reaction in the color environment by Memory and Attention application. By utilizing the reaction of the human body information that is collected thought the proposed system, we can analyze the correlation between the physiological information and the color environment. And we also expect that this system can apply to the medical diagnosis and treatment. For future work, we will expand the system for prediction and treatment of Alzheimer disease by analyzing the visualization data through the proposed system. We will also do evaluation on the effectiveness of the system for using in the rehabilitation program.

• Journal of Korean society of Dental Hygiene
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• v.9 no.2
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• pp.207-220
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• 2009

This study researched into literature materials in order to utilize basic data on job importance in dental coordinator, and analyzed questionnaire on job importance in dental coordinator for about 2 months from August and September in 2008 targeting dental hygienists and other manpower for dental hospitals & clinics where are located in D region and neighboring region. The following are the results: 1. Dental hospital & clinics where dental coordinators work were surveyed to be totally 66.9%. Dental coordinators were indicated to be 71.1% as for dental hospital$^{\circ}{\S}$clinic(women), and to be 28.4% as for a case with none. There was significant difference according to final academic degree and working place(P<.001). 2. License holders(women) for dental hygienist as dental coordinator accounted for 39.8%. The opposite case accounted for 60.2%. A case of doing duty of dental coordinator given not dental hygienist was indicated to be high. There was significant difference according to working place(p<.01). 3. The task importance on job that a dental coordinator directly performs was indicated to be averagely 3.24, thereby having been recognized to be important. 4. As for the recognition on job importance according to working-year number, it was indicated to have higher recognition on task importance in the more working-year number regarding customer management(p<.01), organization management(p<.05), and self-management(p<.01). 5. Given seeing difference in recognition on task importance according to medical institution, it was indicated to be 3.34 for dental clinic and 3.25 for dental hospital. Thus, the task importance was indicated to be slightly high in a person who works for dental clinic. There was no significant difference. In light of the above results, in order to educate dental coordinator who can successfully perform a role at dental hospital & clinic, a dental hygienist is required who is equipped with dental-clinic career rather than a person without a major. A professionally educational program for dental coordinator needs to be developed. Even in a dental coordinator's task, there is necessity for the curricular development and the specialized education.

• Journal of Digital Convergence
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• v.11 no.10
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• pp.469-481
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• 2013

The purpose of this study was to describe working stress, child-rearing stress and parenting attitude of nurses who have preschool children. Methods: Data were collected by questionnaires from 136 nurses in three university hospital in 2011, and analyzed by the SPSS 12.0 program. Results: Nurses scored 3.48 points on average in working stress, 2.47 points in child-rearing stress, 3.61 points in parenting attitude. According to analysis on working stress depending on general demographic characteristics showed significant differences in workplace, monthly weekend duty frequency and in child-rearing stress depending on husband's age, wife's age, total income, marital period, satisfaction at relationship with husband, working career, number of children, and a person who cares for their children. According to parenting attitude depending on general demographic characteristics showed significant differences in working condition, marital period, position, working conditions, marital period, workplace, monthly weekend duty frequency. Correlations among nurses' working stress, child-rearing stress and parenting attitude, nurses' working stress had significantly correlations with parenting stress. Conclusion: Results of the study provides data on nursing interventions to relieve nurses from their working stress and child-rearing stress and to promote favorable child parenting attitude.