• 제목/요약/키워드: health statistical programs

검색결과 395건 처리시간 0.025초

동료평가를 활용한 실습교육평가방법이 핵심기본간호술 수행능력, 학습태도, 비판적사고 및 셀프리더십에 미치는 효과 (The Effects of Practice Education Evaluation using Peer Evaluation on Core Fundamental Nursing Skills Competency, Learning Attitude, Critical thinking and Self-Leadership)

  • 김영희
    • 한국산학기술학회논문지
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    • 제20권9호
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    • pp.351-361
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    • 2019
  • 본 연구는 동료평가를 활용한 핵심기본간호술의 실습교육평가방법이 졸업반 간호학생의 핵심기본간호술 수행능력, 학습태도, 비판적사고 및 셀프리더십에 미치는 효과를 파악하고자 하였다. 연구대상자는 핵심기본간호술 교과목 수강자 4학년 학생으로 총 326명이었으며, 자료수집은 2018년 한 학기동안 실시하였다. 자료는 AMOS 20.0과 SAS 9.3 프로그램을 이용하였으며 t-test, ANOVA, Pearson correlation coefficient, Scheffe test로 분석하였다. 연구결과로, 동료평가를 활용한 핵심기본간호술 수행능력의 평균평점은 3.77(${\pm}.62$)점, 학습태도 3.17(${\pm}.47$)점, 비판적 사고는 3.44(${\pm}.41$)점, 셀프리더십은 3.60(${\pm}.49$)점으로 나타났다. 핵심기본간호술 수행능력은 성별, 간호학 전공만족도, 핵심기본간호술 실습만족도, 동료평가 전 사전학습여부, 동료평가 만족도에 따라 유의한 차이를 나타냈다. 핵심기본간호술 수행능력은 학습태도(r=.295, p<.001)와, 비판적사고는 핵심기본간호술 수행능력(r=.384, p<.001), 학습태도(r=.623, p<.001)와, 셀프리더십은 핵심기본간호술 수행능력(r=.353, p<.001), 학습태도(r=.529, p<.001), 비판적사고(r=.690, p<.001)와 유의한 순상관 관계를 나타냈다. 핵심기본간호술 수행능력에 영향을 미치는 요인은 구조방정식 모형을 적용하였으며 비판적사고, 셀프리더십으로 나타났다. 따라서 이러한 연구결과를 바탕으로 핵심기본간호술 수행능력 향상을 위해 학습태도, 비판적사고 및 셀프리더십을 고려한 핵심기본간호술 실습교육프로그램 개발이 필요할 것이다.

사회적 배제 잠재유형이 노후의 경제적 불안에 미치는 영향: 주관적 계층의식의 조절효과 (The effects of latent classes in social exclusion on the economic instability of old age)

  • 김수진;김주현;주경희
    • 한국노년학
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    • 제40권1호
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    • pp.33-49
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    • 2020
  • 본 연구는 사회적 배제의 잠재유형을 구분하고 노후의 경제적 불안에 미치는 영향을 실증적으로 분석하였다. 또한 주관적 계층의식에 따라 그 영향력이 달라지는지를 함께 살펴보고자 하였다. 이를 위해 2016년 한국종합사회조사(KGSS) 14차 자료를 사용하여, 조사 당시 기준 만 18세의 성인남녀 1,041명의 응답지를 분석하였다. STATA14와 MPLUS 7 통계프로그램을 이용하여 기술통계분석과 t-test, 잠재계층분석(LCA), 잠재집단에 대한 다항로지스틱분석을 실시하였다. 마지막으로 다중회귀분석을 실시하여 변수 간 영향관계 및 조절 효과를 확인하였다. 연구결과 사회적 배제의 유형은 3집단으로 나타났으며, 사회활동배제집단(49.3%), 다차원적 배제집단(30.9%), 사회 활동 적극집단(19.7%)의 순으로 나타났다. 사회활동배제집단은 경제, 고용, 건강 배제의 가능성이 가장 낮지만, 공식/비공식 사회활동의 배제가 두드러진 집단이며, 다차원적 배제집단은 모든 영역에서 배제를 경험할 가능성이 50% 이상인 집단으로 나타났다. 사회활동 적극집단은 비공식 사회활동에 매우 적극적으로 참여하는 것이 특징인 집단이다. 다항로지스틱 분석결과 사회활동배제집단은 다른 집단에 비해 청년층이 많이 포함되었으며, 다차원적 배제집단에는 배우자가 없는 여성노인이 많이 포함되어 있는 것으로 나타났다. 마지막으로 다중회귀분석 결과 사회적 배제 유형은 주관적 계층의식과 상호작용하여 노후의 경제적 불안에 영향을 미치는 것으로 나타났다. 이러한 연구결과를 바탕으로 노후의 경제적 불안을 예방하기 위한 정책적·실천적 방안을 제언하였다.

MZ·X세대의 사회적지지와 노후준비도 관계에서 노인일자리사업 관심도와 회복탄력성의 매개효과 (Mediating Effect of Interest in Elderly Job Project and Resilience on the Relationship between MZ and X Generation's Social Support and Retirement Readiness)

  • 김건희;홍상욱
    • 산업진흥연구
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    • 제7권4호
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    • pp.63-74
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    • 2022
  • 본 연구는 MZ·X세대의 사회적지지와 노후준비도의 관계에서 노인일자리사업 관심도와 회복탄력성의 매개효과를 살펴보는데 목적이 있다. 본 조사는 D광역시와 G도의 G시, Y시, A시의 MZ·X세대 359명의 수집자료를 SPSS 27.0과 AMOS 27.0 프로그램을 사용하여 신뢰도 및 빈도분석, t / F-검증, 기술통계 분석, 상관관계분석, 구조적관계의 통계를 사용하여 분석을 하였다. 연구결과, 첫째, 노인일자리사업 관심도, 사회적지지, 회복탄력성과 노후준비도는 유의미한 상관관계가 있는 것으로 나타났다. 둘째, 사회적지지와 노후준비도 관계에서 노인일자리사업 관심도와 회복탄력성은 부분 매개가 검증되었다. 따라서, MZ·X세대의 노인일자리사업에 대한 관심과 회복탄력성을 높이기 위해서 사회적지지체계로서 정서적, 평가적, 정보력, 물질적 지지체계의 마련과 보건복지부, 고용노동부, 여성가족부와 같은 정부기관의 노인일자리 사업정책의 현실성 있는 정책이 필요하다.

노인의 의미 있는 활동에 관한 융합 연구 (A Convergence Study on the Meaningful Activity Experience of the Elderly)

  • 황혜정;강경희;김두리;장경희;김광환
    • 한국융합학회논문지
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    • 제13권5호
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    • pp.45-52
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    • 2022
  • 이 연구에서는 노년기 의미 있는 활동의 요인을 분석할 목적으로 시행되었으며, 최종연구대상은 65세 이상 110명을 대상으로 자기기입식 설문을 수행하였다. 연구방법은 SPSS 통계프로그램을 이용하여 빈도분석 및 student t-test와 one-way ANOVA를 실시하였으며, 위계적 회귀분석을 통해 노인의 의미 있는 활동에 미치는 요인을 파악하였다. 연구결과 '나는 가족과 함께 하는 일(활동)이 보람 있다고 생각 한다'가 3.95±0.64점으로 가장 높게 나타났다. 위계적 회귀분석 결과 노인의 의미 있는 활동에 미치는 요인은 1.2.3단계 각각 거주지(서울)(β=-.308, p=.002), (β=-.330, p=<.001), (β=-.281, p=<.001)로 나타났고. 2단계에서 연령(β=-.215, p=.026) 나타나 노인의 의미 있는 활동에 미치는 요인은 거주지(서울)와 연령으로 나타났다. 결과적으로 앞으로 노년기 의미 있는 활동을 위해서는 지역을 고려하여 연소노인(65-74세) 대상으로 한 가족과 함께 하는 일(활동)이면서 대화 및 의사소통에 우선한 프로그램의 개발이 필요하다. 추후 노년기 의미 있는 활동을 위한 다양한 프로그램의 체계적인 적용이 필요할 것이다.

우리나라 임상간호사의 당뇨병 지식 및 지식 확산도 조사연구 (Knowledge and Diffusion of Knowledge for Nursing Care of Patients with Diabetes Mellitus among Clinical Nurses)

  • 홍명희;유주화;김순애;이정림;노나리;박정은;구미옥
    • 임상간호연구
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    • 제15권3호
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    • pp.61-74
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    • 2009
  • Purpose: In order to increase the quality of nursing care for patients with diabetes mellitus, it is important for clinical nurses to accept changes in diabetes knowledge and correct their approach immediately. This approach will also contribute to effective nursing practice. Methods: The study was designed to investigate the level of knowledge and diffusion of knowledge for nursing care of patients with diabetes mellitus among clinical nurses. It was conducted with nurses from 29 general hospitals in Korea from November 3 to December 5, 2008. The questionnaire consisted of 129 items and it was sent to the participants by mail. Of the 1,060 questionnaires returned, only 930 were valid for use in the statistical analysis. Results: 1) The average score for clinical nurses' knowledge of diabetes mellitus was 0.67 out of 1.0. 2) The level of persuasion of knowledge for nursing care of patients with diabetes mellitus averaged 0.64 out of 1.0 3) The level of practical application of knowledge for nursing care of patients with diabetes mellitus averaged 1.05 out of 2.0, indicating that they applied their knowledge 'sometimes'. 4) The level of diffusion of knowledge for nursing care of patients with diabetes mellitus was 2.37 out of 4.0 and level was estimated as the stage of 'persuasion'. 5) There were significant differences in nursing knowledge of diabetes mellitus, according to experience in practical education for diabetes mellitus. Conclusion: The results indicate that nurses with a lower level of knowledge of diabetes mellitus have a lower level of persuasion of knowledge for nursing care of patients with diabetes mellitus and lower practical application. To improve the level of nurses' knowledge of diabetes mellitus, practical training programs are needed for areas in which knowledge level is low, such as 'diagnosis and management of diabetes mellitus', 'oral diabetes medication', and 'glucose control in special conditions'.

지역사회의 여가활성화 요인 탐색 연구 (A Study on Factors to Activate Community Leisure Activities)

  • 전형상
    • 한국체육학회지인문사회과학편
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    • 제54권3호
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    • pp.421-437
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    • 2015
  • 본 연구는 지역사회의 여가활성화 요인을 탐색하고 중요도를 산출하여, 정부와 광역정부에 기초적인 자료를 제시하는데 목적이 있다. 이러한 목적을 달성하기 위하여 전문가 인터뷰 및 선행연구분석, 지역주민의 개방형 설문을 통한 질적연구와 실증적 연구를 위한 인천, 대전, 광주, 부산 4개 권역별 지역주민을 대상으로 100부씩 400부를 배포한 후 수거하여 SPSS 18.0을 이용한 기술통계분석, 탐색적요인분석, 신뢰도분석을 토대로 양적연구를 수행한 결과 다음과 같이 나타났다. 첫째, 전문가 면접을 통해 15개의 요인이 추출되었으며, 개방형설문과 전문가의견, 기존선행연구 결과를 통해 시설, 프로그램, 조직 및 전문인력, 홍보, 정책, 기타의 6개 요인으로 추출되었다. 둘째, 추출된 6개의 요인을 통해 지역주민이 인식하는 여가활성화 필요 요인 도출을 위해 400부의 설문을 분석한 결과 6개 요인이 요인분석을 통해 시설 요인 4개, 여가정책과 기타요인이 각각 2개의 요인으로 분류되었으며, 프로그램, 조직 및 전문인력 각각 1개 요인으로 추출되었으나 홍보요인은 문항 전체가 타당도와 신뢰도가 결여되어 삭제를 통해 총 10개의 요인으로 추출되었다. 셋째, 각 문항별 중요도는 대부분의 문항에서 5점 이상의 평균값을 나타냈다.

가족계획과 모자보건 통합을 위한 조산원의 투입효과 분석 -서산지역의 개입연구 평가보고- (An Intervention Study on Integration of Family Planning and Maternal/Infant Care Services in Rural Korea)

  • 방숙;한성현;이정자;안문영;이인숙;김은실;김종호
    • Journal of Preventive Medicine and Public Health
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    • 제20권1호
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    • pp.165-203
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    • 1987
  • This project was a service-cum-research effort with a quasi-experimental study design to examine the health benefits of an integrated Family Planning (FP)/Maternal & Child health (MCH) Service approach that provides crucial factors missing in the present on-going programs. The specific objectives were: 1) To test the effectiveness of trained nurse/midwives (MW) assigned as change agents in the Health Sub-Center (HSC) to bring about the changes in the eight FP/MCH indicators, namely; (i)FP/MCH contacts between field workers and their clients (ii) the use of effective FP methods, (iii) the inter-birth interval and/or open interval, (iv) prenatal care by medically qualified personnel, (v) medically supervised deliveries, (vi) the rate of induced abortion, (vii) maternal and infant morbidity, and (viii) preinatal & infant mortality. 2) To measure the integrative linkage (contacts) between MW & HSC workers and between HSC and clients. 3) To examine the organizational or administrative factors influencing integrative linkage between health workers. Study design; The above objectives called for quasi-experimental design setting up a study and control area with and without a midwife. An active intervention program (FP/MCH minimum 'package' program) was conducted for a 2 year period from June 1982-July 1984 in Seosan County and 'before and after' surveys were conducted to measure the change. Service input; This study was undertaken by the Soonchunhyang University in collaboration with WHO. After a baseline survery in 1981, trained nurses/midwives were introduced into two health sub-centers in a rural setting (Seosan county) for a 2 year period from 1982 to 1984. A major service input was the establishment of midwifery services in the existing health delivery system with emphasis on nurse/midwife's role as the link between health workers (nurse aids) and village health workers, and the referral of risk patients to the private physician (OBGY specialist). An evaluation survey was made in August 1984 to assess the effectiveness of this alternative integrated approach in the study areas in comparison with the control area which had normal government services. Method of evaluation; a. In this study, the primary objective was first to examine to what extent the FP/MCH package program brought about changes in the pre-determined eight indicators (outcome and impact measures) and the following relationship was first analyzed; b. Nevertheless, this project did not automatically accept the assumption that if two or more activities were integrated, the results would automatically be better than a non-integrated or categorical program. There is a need to assess the 'integration process' itself within the package program. The process of integration was measured in terms of interactive linkages, or the quantity & quality of contacts between workers & clients and among workers. Intergrative linkages were hypothesized to be influenced by organizational factors at the HSC clinic level including HSC goals, sltrurture, authority, leadership style, resources, and personal characteristics of HSC staff. The extent or degree of integration, as measured by the intensity of integrative linkages, was in turn presumed to influence programme performance. Thus as indicated diagrammatically below, organizational factors constituted the independent variables, integration as the intervening variable and programme performance with respect to family planning and health services as the dependent variable: Concerning organizational factors, however, due to the limited number of HSCs (2 in the study area and 3 in the control area), they were studied by participatory observation of an anthropologist who was independent of the project. In this observation, we examined whether the assumed integration process actually occurred or not. If not, what were the constraints in producing an effective integration process. Summary of Findings; A) Program effects and impact 1. Effects on FP use: During this 2 year action period, FP acceptance increased from 58% in 1981 to 78% in 1984 in both the study and control areas. This increase in both areas was mainly due to the new family planning campaign driven by the Government for the same study period. Therefore, there was no increment of FP acceptance rate due to additional input of MW to the on-going FP program. But in the study area, quality aspects of FP were somewhat improved, having a better continuation rate of IUDs & pills and more use of effective Contraceptive methods in comparison with the control area. 2. Effects of use of MCH services: Between the study and control areas, however, there was a significant difference in maternal and child health care. For example, the coverage of prenatal care was increased from 53% for 1981 birth cohort to 75% for 1984 birth cohort in the study area. In the control area, the same increased from 41% (1981) to 65% (1984). It is noteworthy that almost two thirds of the recent birth cohort received prenatal care even in the control area, indicating that there is a growing demand of MCH care as the size of family norm becomes smaller 3. There has been a substantive increase in delivery care by medical professions in the study area, with an annual increase rate of 10% due to midwives input in the study areas. The project had about two times greater effect on postnatal care (68% vs. 33%) at delivery care(45.2% vs. 26.1%). 4. The study area had better reproductive efficiency (wanted pregancies with FP practice & healthy live births survived by one year old) than the control area, especially among women under 30 (14.1% vs. 9.6%). The proportion of women who preferred the 1st trimester for their first prenatal care rose significantly in the study area as compared to the control area (24% vs 13%). B) Effects on Interactive Linkage 1. This project made a contribution in making several useful steps in the direction of service integration, namely; i) The health workers have become familiar with procedures on how to work together with each other (especially with a midwife) in carrying out their work in FP/MCH and, ii) The health workers have gotten a feeling of the usefulness of family health records (statistical integration) in identifying targets in their own work and their usefulness in caring for family health. 2. On the other hand, because of a lack of required organizational factors, complete linkage was not obtained as the project intended. i) In regards to the government health worker's activities in terms of home visiting there was not much difference between the study & control areas though the MW did more home visiting than Government health workers. ii) In assessing the service performance of MW & health workers, the midwives balanced their workload between 40% FP, 40% MCH & 20% other activities (mainly immunization). However, $85{\sim}90%$ of the services provided by the health workers were other than FP/MCH, mainly for immunizations such as the encephalitis campaign. In the control area, a similar pattern was observed. Over 75% of their service was other than FP/MCH. Therefore, the pattern shows the health workers are a long way from becoming multipurpose workers even though the government is pushing in this direction. 3. Villagers were much more likely to visit the health sub-center clinic in the study area than in the control area (58% vs.31%) and for more combined care (45% vs.23%). C) Organization factors (admistrative integrative issues) 1. When MW (new workers with higher qualification) were introduced to HSC, it was noted that there were conflicts between the existing HSC workers (Nurse aids with less qualification than MW) and the MW for the beginning period of the project. The cause of the conflict was studied by an anthropologist and it was pointed out that these functional integration problems stemmed from the structural inadequacies of the health subcenter organization as indicated below; i) There is still no general consensus about the objectives and goals of the project between the project staff and the existing health workers. ii) There is no formal linkage between the responsibility of each member's job in the health sub-center. iii) There is still little chance for midwives to play a catalytic role or to establish communicative networks between workers in order to link various knowledge and skills to provide better FP/MCH services in the health sub-center. 2. Based on the above findings the project recommended to the County Chief (who has power to control the administrative staff and the technical staff in his county) the following ; i) In order to solve the conflicts between the individual roles and functions in performing health care activities, there must be goals agreed upon by both. ii) The health sub·center must function as an autonomous organization to undertake the integration health project. In order to do that, it is necessary to support administrative considerations, and to establish a communication system for supervision and to control of the health sub-centers. iii) The administrative organization, tentatively, must be organized to bind the health worker's midwive's and director's jobs by an organic relationship in order to achieve the integrative system under the leadership of health sub-center director. After submitting this observation report, there has been better understanding from frequent meetings & communication between HW/MW in FP/MCH work as the program developed. Lessons learned from the Seosan Project (on issues of FP/MCH integration in Korea); 1) A majority or about 80% of the couples are now practicing FP. As indicated by the study, there is a growing demand from clients for the health system to provide more MCH services than FP in order to maintain the achieved small size of family through FP practice. It is fortunate to see that the government is now formulating a MCH policy for the year 2,000 and revising MCH laws and regulations to emphasize more MCH care for achieving a small size family through family planning practice. 2) Goal consensus in FP/MCH shouBd be made among the health workers It administrators, especially to emphasize the need of care of 'wanted' child. But there is a long way to go to realize the 'real' integration of FP into MCH in Korea, unless there is a structural integration FP/MCH because a categorical FP is still first priority to reduce the rate of population growth for economic reasons but not yet for health/welfare reasons in practice. 3) There should be more financial allocation: (i) a midwife should be made available to help to promote the MCH program and coordinate services, (in) there should be a health sub·center director who can provide leadership training for managing the integrated program. There is a need for 'organizational support', if the decision of integration is made to obtain benefit from both FP & MCH. In other words, costs should be paid equally to both FP/MCH. The integration slogan itself, without the commitment of paying such costs, is powerless to advocate it. 4) Need of management training for middle level health personnel is more acute as the Government has already constructed 90 MCH centers attached to the County Health Center but without adequate manpower, facilities, and guidelines for integrating the work of both FP and MCH. 5) The local government still considers these MCH centers only as delivery centers to take care only of those visiting maternity cases. The MCH center should be a center for the managment of all pregnancies occurring in the community and the promotion of FP with a systematic and effective linkage of resources available in the county such as i.e. Village Health Worker, Community Health Practitioner, Health Sub-center Physicians & Health workers, Doctors and Midwives in MCH center, OBGY Specialists in clinics & hospitals as practiced by the Seosan project at primary health care level.

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라이프스타일 재설계 프로그램이 뇌졸중 노인의 시간 사용과 삶의 질에 미치는 효과 (The Effects of Lifestyle Redesign Program on Time Usage and Quality of Life for Elderly with Stroke)

  • 김형민;전병진
    • 대한지역사회작업치료학회지
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    • 제9권3호
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    • pp.21-30
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    • 2019
  • 목 적 : 본 연구는 작업 균형(Occupational Balance)과 성공적인 노화연구(Well Elderly Research Study)를 이론적 근거로 라이프스타일 재설계 프로그램을 적용하여 뇌졸중 노인의 시간 사용과 삶의 질에 미치는 효과를 확인하고자 하였다. 연구방법 : 요양병원에 거주하는 뇌졸중 노인 총 40명(실험군 20명, 대조군 20명)을 대상으로 통제집단 사전사후검사 설계(pretest-posttest control group design)를 시행하였다. 라이프스타일 재설계 프로그램에 참여하기 전 2주 동안 사전평가를 실시하였고, 이 후 10주 동안 주 7회 라이프스타일 재설계 프로그램을 적용하였으며, 이 후 1주 동안은 사후 평가를 실시하였다. 대상자의 시간 사용은 통계청(2009) 생활시간 조사표를 사용하였고, 삶의 질의 경우 세계보건기구 삶의 질 평가도구(WHOQOL-BREF)를 사용하였다. 결과 : 프로그램 적용 전과 후의 시간 사용량 비교 결과 실험군의 경우 휴식과 수면(t=-4.89, p<.001), 여가(t=-4.67, p<.001)에서 통계적으로 유의한 차이를 확인할 수 있었다. 또한 집단 간 비교 결과 역시 휴식과 수면(t=-2.24, p<.01) 그리고 여가(t=3.57, p<.01)에서 통계적으로 유의한 차이가 나타났다. 삶의 질 비교 결과 두 그룹 간 통계적으로 유의한 차이가 나타났다(t=6.80, p<.001). 삶의 질 하위영역을 살펴보면 신체적 건강영역(t=6.08, p<.001), 심리적 건강영역(t=5.21, p<.001), 생활 환경영역(t=3.60, p<.01)에서 통계적으로 유의한 차이를 확인할 수 있었다. 결론 : 라이프스타일 재설계 프로그램은 요양병원에 거주하는 뇌졸중 노인의 휴식과 수면의 시간 사용은 줄이고, 여가생활에 참여하는 시간 사용을 늘려주었으며, 그로 인해 삶의 질이 향상되는 결과를 나타내었다.

가정배달 노인급식 수혜자의 위생지식 및 가정에서의 위생관리 습관 (Food Safety Knowledge and Home Food Safety Practices of Home-delivered Meal Service Recipients)

  • 이경은;이나영;박정연
    • 한국식품영양과학회지
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    • 제38권5호
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    • pp.618-625
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    • 2009
  • 본 연구의 목적은 가정배달 노인급식 수혜자의 위생지식수준과 가정에서의 위생관리 수행도를 조사하는 것으로 서울지역에서 가정배달 노인급식서비스를 제공하는 노인복지관과 종합사회복지관 2곳을 선정하고 수혜자를 무작위로 선정하여 설문조사를 실시하였다. 총 120명을 선정하였고 조사에 참여한 97명의 응답을 자료 분석에 이용하였다. 응답자의 대부분은 여성이었고, 70세 이상이었으며, 초등학교 졸업이하의 학력을 보였다. 절반가량이 혼자 살고 있었고, 여러가지 만성질환으로 약을 복용하고 있었으며 스스로도 자신의 건강을 좋지 않게 인식하고 있었다. 위생지식 평가 결과 손 씻기의 필요성은 인식하고 있었으나 그 구체적인 방법에 대한 지식은 부족하였고, 가열처리 없이 섭취하는 과일의 세척, 소독 방법, 남은 음식의 보관 방법, 행주의 사용에 대한 지식수준이 낮게 나타났다. 배달급식을 받는 노인들의 가정 내에서 위생관리 수행도 평균 점수는 2.8점으로 높지 않았다. 남은 음식의 완전한 재가열, 유통기한 준수가 가장 잘 수행되고 있는 반면, 손 상처의 부적절한 처리, 행주와 도마의 관리, 달걀 등 오염도가 높은 식품 취급 후 손 씻기가 잘 수행되고 있지 않았다. 급식 수혜노인의 위생지식과 식품 취급습관은 일부를 제외하고는 일반 사항에 따른 유의적인 차이가 없었다. 위생지식 총점과 수행도 평균은 유의적인 양의 상관관계를 보였고(p<0.01), 세부 영역별로는 구분사용과 세척 및 소독 위생지식이 관련 수행도와 유의적인 양의 상관관계를 보였다(p<0.01). 독거노인과 노인가정이 증가하면서 가정에서 노인들의 식품취급은 증가할 것이고, 외부에서 조리된 음식을 가정에서 섭취할 경우에도 섭취 전까지 보관이 식품의 안전성 확보에 중요하므로 노인들을 대상으로 하는 위생교육 프로그램의 개발에 본 연구 결과가 이용될 수 있을 것이다. 보다 효과적인 위생교육 프로그램의 개발을 위해서는 위생교육을 통해 습득된 위생지식이 실생활에서 실천되는 과정에 영향을 미칠 수 있는 요인들에 대한 연구 역시 지속되어야 할 것이다.

급성(急性) 기아(饑餓)마우스의 간단백질(肝蛋白質), 핵산(核酸) 및 Guanine Deaminase 활성(活性)에 관(關)한 연구(硏究) (A Study on The Content of Liver Protein, Nucleic Acids, and Guanine Deaminase Activity of Mouse During Acute Starvation)

  • 박승희;김승원
    • Journal of Nutrition and Health
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    • 제1권2호
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    • pp.107-115
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    • 1968
  • Number of aspects, not only nutritional but social as well as political involved in human starvation pose nowadays global problems. In order to help establish the minimum nutritional requirements in the daily life of a man and to free people as well from either undernourishment, malnutrition or even starvation many workers have devoted themselves so far on the research programs to know what and how number of metabolic events take place in animals in vivo. It is the purpose of the present paper to examine in effect to what extent both of the protein and nucleic acids (DNA & RNA) together with an enzyme, guanine deaminase, which converts guanine into xanthine and in turn ends up to uric acid as an end product, undergo changes, quantitatively during acute starvation, using the mouse as an experimental animal. The mouse was strictly inhibited from taking foods except drinking water ad libitum and was sacriflced 24, 48, and 72 hours following starvation thus acutely induced. The animals consisted of two experimental groups, one control and another starvation groups, each being consisted of 6-24 mice of whose body weights ranged in the vicinity of 10 g. The animals were sacriflced by a blow on the head, followed by immediate excision of their livers into ice-cold distilled water, washing adherent blood and other contaminant tissues. The liver was minced foramin, by an all-glass homogenizer immersing it in an ice-bath, followed by subsequent fractionatin of the homogenate (10% W/V in 0.25M sucrose solution made up with 0.05M phosphate buffer of pH 7.4). For the liver protein and guanine deaminase assay, the 10% homogenate was centrifuged at 600 x g for 10 minutes to eliminate the nuclear fraction; and for the estimation of DNA and RNA, the homogenate was prepared by the addition of 10% trichloroacetic acid in order to free the homogenate from the acid-soluble fraction, the remaining residue being delipidate by the addition of alcohol and dried in vacuo for later KOH (IN) hydrolysis. The changes in body and liver wegihts during acute starvation were checked gravimetrically. Protein contents in the liver were monitored by the method of Lowry et al; and guanine deaminase activities were followed by the assay of liberated ammonia from the substrate utilizing the Caraway's colorimetry. The extraction of both DNA and RNA was performed by the Schmidt-Thannhauser's method, which was followed by Marmur's method of purification for DNA and by Chargaff's method of purification for RNA. The determinations of both DNA and RNA were carried out by the diphenylamine reaction for the former and by the orcinol reaction for the latter. The following resume was the results of the present work. 1. It was observed that the body as well as liver weights fall abruptly during starvation, and that the loss of body weight showed no statistical correlation with the decreases in the content of liver protein. 2. The content of liver protein and activity of liver guanine deaminase activity as well decline dramatically, and the specific activities of the enzyme (activity/protein), however, decreased gradually as starvation proceeded. 3. Both of the nucleic acids, DNA and RNA, showed decrements in the liver of mouse during acute starvation; the latter, however, being more striking in the decline as compared to the former. 4. The decreases in the liver protein content as resulted from the acute starvation had no statistically significant correlation with the decrements of DNA in the same tissue, but had regressed with a significant statistical correlation with the fall of RNA in the tissue. 5. The decrease in the activity of guanine deaminase in the liver of mouse during acute starvation was functionally more proportional to the decrease in RNA than DNA, and moreover correlated with the changes in the content of the liver protein. 6. The possible mechanisms involved during in this acute starvation as bring the decreases in the contents of DNA, protein, and guanine deaminase were discussed briefly.

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