• Molecules and Cells
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• v.19 no.2
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• pp.198-204
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• 2005

Both erythropoietin (EPO) and the short-form thrombopoietin (TPO) were expressed at low levels whereas the long-form TPO was expressed at high levels in transgenic animals. To elucidate the role of carboxy-terminal half of the long-form TPO which is absent in the short-form, we generated recombinant TPO or EPO expression vectors which contain or lack the carboxy-terminal half of TPO and examined their expression in the HC11 and 293 cells. The long-form TPO was expressed higher than the short-form regardless of the cell types, transfection modes, and promoters. When 3'-half of the long-form TPO cDNA was placed downstream of the EPO cDNA to act as a 3'-untranslated region, expression of EPO was moderately increased at the RNA level, however, no remarkable increase was observed at the protein level. These results suggest that the low expression of EPO, as like as the short-form TPO, is due to absence of the 3'-half in the full-length TPO that confers stability both at the RNA and protein levels.

• Korean Journal of Bronchoesophagology
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• v.7 no.1
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• pp.54-58
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• 2001

Granular cell tumor or also called Abrikossoff's tumor, is a rare benign tumor that can occur anywhere in the body. Granular cell tumor may single or multiple (synchronous or metachronous). Approximately half of all granular cell tumors occur in the head and neck. most commonly in the tongue. But granular cell tumors of the larynx are rare, accounting for only 7 to 10% of all reported cases. Laryngeal tumors are usually small and men are more frequently affected than women. The exact histogenesis is still not known but most recent data support the neurogenic Schwann cell origin. The preferred treatment is local excision using cold knife or laser. Radiation therapy is of little benefit. We report a case of granular cell tumor of the larynx which was confirmed by histopathology.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.2
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• pp.207-216
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• 1998

Certain types of somatic cells, as well as follicular cumulus cells associating with mammalian oocytes, are known to produce beneficial effects on in vitro fertilization and pre implantation development of mammalian eggs when they are present in oocyte culture medium. To investigate the nature of the effects of somatic cells, the resistance of mouse oocytes against chymotrypsin treatment was examined after culture within various cell conditioned media. When mouse oocytes matured for 17-18 hr in the presence of cumulus cells were treated with 1 % chymotrypsin, half of them remained still alive even after 240 min $(t_{50}>240.0)$. In contrast half of mouse oocytes cultured without cumulus cells underwent degeneration within 65.0 min $(t_{50}=65.0{\pm}13.2min)$ of the same treatment. To see if the effects were duc to the secretory products of cumulus cells, mouse cumulus cells were cultured for 20 hr in medium containing 0.4% BSA and the supernatant of culture medium (conditioned medium) was taken. After maturation in the cumulus cell conditioned medium, mouse oocytes exhibited $t_{50}=190.0{\pm}10.8$ min upon chymotrypsin treatment whereas half of oocytes cultured without conditioned medium degenerated within 25.5 min. Human granulosa cell conditioned medium gave similar effects such that oocytes matured in conditioned medium exhibited $t_{50}=183.3{\pm}19.1$ min while $t_50$ of control group oocytes was $60.0{\pm}6.8$ min, Oocytes matured in vero cell conditioned medium exhibited $t_{50}=196.7{\pm}8.8$ min. On the other hand, amniotic cell conditioned medium resulted in the chymotrypsin resistance of $t_{50}=80.0{\pm}8.4$ min which was not statistically different from the control value of $t_{50}=48.0{\pm}13.2$ min. Based upon these results, it is suggested that certain somatic cell types including cumulus cells might change the biochemical properties of mouse oocyte membrane during meiotic maturation as revealed by the enhanced resistance against chymotrypsin treatment. Such effects of somatic cells appear to be mediated via the secretory products rather than direct communication between somatic cells and oocytes.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1999.05a
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• pp.634-637
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• 1999

The amorphous vanadium oxide as a cathode material is very preferable for fabricating high performance micro-battery. The amorphous vanadium oxide cathode is preferred over the crystalline form because three times more lithium ions can be inserted into the amorphous cathode, thus making a battery that has a higher capacity. The electrochemical properties of sputtered films are strongly dependent on the oxygen partial pressure in the sputtering gas. The effect of different oxygen partial pressure on the electrochemical properties of vanadium oxide thin films formed by r.f. reactive sputtering deposition were investigated. The stoichiometry of the as-deposited films were investigated by Auger electro spectroscopy. X-ray diffraction and atomic force microscopy measurements were carried out to investigate structural properties and surface morphology, respectively. For high oxygen partial pressure(>30% ), the films were polycrystalline V$_2$O$_{5}$ while an amorphous vanadium oxide was obtained at the lower oxygen partial pressure(< 15%). Half-cell tests were conducted to investigate the electrochemical properties of the vanadium oxide film cathode. The cell capacity was about 60 $\mu$ Ah/$\textrm{cm}^2$ m after 200 cycle when oxygen partial pressure was 20%. These results suggested that the capacity of the thin film battery based on vanadium oxide cathode was strongly depends on crystallinity.y.

• Journal of the Korean Society of Hazard Mitigation
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• v.2 no.1 s.4
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• pp.143-152
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• 2002

The objectives of this paper were to obtain the fundamental data to analyze the causes of deterioration of 39 freeway concrete viaducts in Seoul metropolitan area. To investigate the degree of concrete deterioration, carbonation depth, soluble chloride concentration in hardened concrete and half-cell potentials of reinforcement were measured. The number of structures which carbonation depth penetrates to reinforcement was 25% of total. The model of carbonation .ate was induced to 3.92 $\sqrt{t}$, which was 5% faster than 3.727 $\sqrt{t}$ assumed 60% water-cement ratio, R=1 in that of kishitani. After measuring chloride concentration in concrete, it was concluded that about 24% of all readings on samples from concrete exceed the critical content to minimize the risk of chloride-induced corrosion. About 31% of the freeway viaducts structures had a value lower than -350mV(vs. CSE), so it could conclude that the excessive chloride concentration was the major cause of reinforcement corrosion. Among the structures which measured half-cell potentials less than -350mV, about 50% exceeds the maximum acceptable limit of chloride concentration.

• Journal of the Korea institute for structural maintenance and inspection
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• v.17 no.6
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• pp.95-103
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• 2013

Half Cell Potential (HPC) technique has been widely adopted for its quantitative evaluation of corrosion possibility. In this study, RC specimens with three different cover depths (10mm, 30mm, and 60mm) and w/c ratios (0.35, 0.55, and 0.70) are prepared and accelerated salt spray test (SST) is performed for 45 days. Steel corrosion occurs in the specimens with 0.55 of w/c and 10mm of cover depth. In the case of 0.70 of w/c and 30mm of cover depth, steel corrosion is also monitored. Considering the effect of cover depth and w/c ratio, HCP evaluation equation is proposed and the condition which can control steel corrosion is obtained. Furthermore, anti-corrosive conditions containing w/c ratio and cover depth are analyzed through Life 365 program and the conditions are compared with the results from this study.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.2
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• pp.75-81
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• 2010

In this study, when concrete properties are changed by concrete mix proportions or blending of admixtures, the characteristics of electro-chemical method for corrosion assessment of the embedded steel are compared and its causes are analyzed. According to the results, when the ratio of corroding area was less than 10%, the half-cell method was affected by concrete properties. In the case of specimen blended admixtures, it is possible to assess the high-corroded steel qualitatively using the half-cell method. For the polarization resistance method, though the corroding area was less than 10%, it has not affected by concrete properties. However, in case of specimen blended admixtures, the corrosion level of steel was underestimated than OPC specimens having a similar corroding area.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.3
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• pp.307-313
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• 2022

In this study, the correlation between Half Cell Potential(HCP) and the corrosion influencing factors was analyzed with considering three levels of water-cement ratio, the concentration of chloride solution, and cover depth. As a result of long-term corrosion monitoring, HCP behavior was close to the critical corrosion potential(-350 mV) in all water-cement ratios in the case of 3.5 % and 7.0 % chloride concentration. Regarding the passed charge test in 548 curing days, the passed charge results were improved to 'Moderate' grade. Multiple regression analysis was performed to evaluate the correlation between corrosion influencing factors and HCP, and it was evaluated that the effects of influencing factors to HCP were in the order of chloride concentration, water-cement ratio, and cover depth. In the case of the relationship between HCP and the passed charge, the coefficient of determination showed a high level of 0.9, which yielded a close correlation between the passed charge and HCP.

• Tuberculosis and Respiratory Diseases
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• v.40 no.3
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• pp.301-307
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• 1993

Intravascular bronchioloalveolar tumor is now recognized as a pulmonary form of hemangioendothelioma(HE). HE is an unusual tumor of adult life which is characterized by proliferation of an "epithelioid" or "spindle" endothelial cell. In the lung it usually presents as multiple bilateral slowly growing nodules less than 2 cm in diameter. The aetiology and pathogenesis of this disease are unknown. Spindle cell HE occurs at any age, but approximately one half of patient are 25 years of age or younger and males are affected twice more frequently than females. On light microscopic examination, the tumor show mild cellular atypia, nearly absent mitoses and electron-microscopic studies reveal evidence of endothelial cell differentiation. Intracytoplasmic localization of Factor VIII-related antigen is demonstrated on immunohistochemical study, which confirmed the endothelial origin of the tumor. No effective therapy is yet known for HE, but survival of this tumor can be quite long. However, one half of the patient have died, usually of progressive pulmonary insufficiency. This 19-yr-old male complained of Rt. chest pain and intermittent hemoptysis. Simple chest film and chest CT scan showed the Rt. pleural effusion, variable sized bilateral pulmonary nodules, irregular large heterogenous tumor with well enhancement and extensive necrosis in the anterior mediastinum. The mediastinal mass was biopsied and diagnosed as spindle cell HE by light microscopic finding and immunohistochemical studies.

• Journal of Electrochemical Science and Technology
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• v.5 no.1
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• pp.1-18
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• 2014

Li-air cell is an exotic type of energy storage and conversion device considered to be half battery and half fuel cell. Its successful commercialization highly depends on the timely development of key components. Among these key components, the catalyst (i.e., the core portion of the air electrode) is of critical importance and of the upmost priority. Indeed, it is expected that these catalysts will have a direct and dramatic impact on the Li-air cell's performance by reducing overpotentials, as well as by enhancing the overall capacity and cycle life of Li-air cells. Unfortunately, the technological advancement related to catalysts is sluggish at present. Based on the insights gained from this review, this sluggishness is due to challenges in both the commercialization of the catalyst, and the fundamental studies pertaining to its development. Challenges in the commercialization of the catalyst can be summarized as 1) the identification of superior materials for Li-air cell catalysts, 2) the development of fundamental, material-based assessments for potential catalyst materials, 3) the achievement of a reduction in both cost and time concerning the design of the Li-air cell catalysts. As for the challenges concerning the fundamental studies of Li-air cell catalysts, they are 1) the development of experimental techniques for determining both the nano and micro structure of catalysts, 2) the attainment of both repeatable and verifiable experimental characteristics of catalyst degradation, 3) the development of the predictive capability pertaining to the performance of the catalyst using fundamental material properties. Therefore, under the current circumstances, it is going to be an extremely daunting task to develop appropriate catalysts for the commercialization of Li-air batteries; at least within the foreseeable future. Regardless, nano materials are expected to play a crucial role in this field.