• 제목/요약/키워드: gyrA

검색결과 161건 처리시간 0.034초

Color Ratios of Parallel-Component Polarization as a Maturity Indicator for the Lunar Regolith

  • Kim, Sungsoo S.;Jung, Minsup;Sim, Chae Kyung;Kim, Il-Hoon;Park, So-Myoung;Jin, Ho
    • 천문학회보
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    • 제40권1호
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    • pp.62.1-62.1
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    • 2015
  • Polarization of the light reflected off the Moon provides information on the size and composition of the particles in the lunar regolith. The mean particle size of the regolith can be estimated from the combination of the albedo and degree of polarization, while the color ratio of the parallel-component polarization (CP) has been suggested to be related to the amount of nanophase metallic iron (npFe^0) inside the regolith particles. Both the mean size and npFe^0 abundance of the particles have been used as maturity indicators of the regolith since sustained impacts of high energy particles and micro-meteoroids cause comminution of particles and production of npFe^0. Based on our multispectral polarimetric observations of the whole near side of the Moon in the U, B, V, R, and I bands, we compare the maps of the mean particle size, CP, and the optical maturity (OM). We find that the mean particle size map is sensitive to the most immature (~0.1 Gyr) soil, the OP map to the intermediate immaturity (a few 0.1 Gyr) soil, and the CP map to the least immature (~1 Gyr) soil.

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Star-Gas Misalignment in Galaxies: II. Origins Found from the Horizon-AGN Simulation

  • Khim, Donghyeon J.;Yi, Sukyoung K.
    • 천문학회보
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    • 제46권1호
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    • pp.29.1-29.1
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    • 2021
  • There have been many studies aiming to reveal the origins of the star-gas misalignment found in galaxies, but there still is a lack of understanding of the contribution from each formation channel candidate. We explore the properties, origins, and lifetimes of the star-gas misalignment using Horizon-AGN, a large-volume cosmological simulation. First, the misalignment fraction shows a strong anti-correlation with the kinematic morphology (V/sigma) and the cold gas fraction of the galaxy. This result is consistent with the result of integral field spectroscopy observations. Second, we have identified four main formation channels of misalignment and quantified their level of contribution: mergers (35%), interaction with nearby galaxies (23%), interaction with dense environments or their central galaxies (21%), and secular evolution including smooth accretion from neighboring filaments (21%). Third, the decay timescale of the misalignment is strongly linked with the kinematic morphology of the galaxy: early-type galaxies (2.28 Gyr) tend to have a longer misalignment lifetime than LTGs (0.49 Gyr). We also found that the morphology and cold gas fraction are both and independently anti-correlated with the misalignment lifetime.

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Evolution of Galaxy Habitability

  • Hong, Sungwook E.;Gobat, Raphael
    • 천문학회보
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    • 제41권2호
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    • pp.53.3-54
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    • 2016
  • We combine a semi-analytic model of galaxy evolution with constraints on circumstellar habitable zones and the distribution of terrestrial planets in order to probe the suitability of galaxies of different mass and type to host habitable planets, and how it evolves with time. We find that the fraction of stars with terrestrial planets in their habitable zone (known as habitability) depends only weakly on galaxy mass, with a maximum around $4{\times}10^{10}M_{\odot}$. We estimate that 0.7% of all stars in Milky Way-type galaxies to host a terrestrial planet within their habitable zone, consistent with the value derived from Kepler observations. On the other hand, the habitability of passive galaxies is slightly but systematically higher, unless we assume an unrealistically high sensitivity of planets to supernovae. We find that the overall habitability of galaxies has not changed significantly in the last ~8 Gyr, with most of the habitable planets in local disk galaxies having formed ~1.5 Gyr before our own solar system. Finally, we expect that ${\sim}1.4{\times}10^9$ planets similar to present-day Earth have existed so far in our galaxy.

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Mutations in the GyrA Subunit of DNA Gyrase and the ParC Subunit of Topoisomerase IV in Clinical Strains of Fluoroquinolone-Resistant Shigella in Anhui, China

  • Hu, Li-Fen;Li, Jia-Bin;Ye, Ying;Li, Xu
    • Journal of Microbiology
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    • 제45권2호
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    • pp.168-170
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    • 2007
  • In this research 26 Shigella isolates were examined by PCR and direct nucleotide sequencing for genetic alterations in the quinolone-resistance determining regions (QRDRs). We tested for the presence of qnr genes by PCR in 91 strains, but no qnr genes were found. The results did show, however, some novel mutations at codon 83 of gyrA ($Ser{\rightarrow}Ile$) and codon 64 of parC ($Ala64{\rightarrow}Cys,\;Ala64{\rightarrow}Asp$), which were related to fluroquinolone resistance.

충청지역의 임상검체로부터 분리된 Acinetobacter calcoaceticus-baumannii Complex를 대상으로 항균제 내성 유전자 비교분석 (Distribution of Antimicrobial Resistant Genes in Acinetobacter calcoaceticus-baumannii Complex Isolated from Clinical Specimens in Chungcheong, Korea)

  • 성지연
    • 대한임상검사과학회지
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    • 제49권4호
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    • pp.427-434
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    • 2017
  • Acinetobacter calcoaceticus-baumannii (Acb) complex에 속한 종들은 빈번하게 병원감염 및 기회감염을 일으킨다. 또한 다제내성인 경우가 많아 이 균들의 감염증 치료를 위한 항균제 선택이 매우 제한적이다. 본 연구에서는 ciprofloxacin 내성 Acinetobacter species 53균주를 대상으로 fluoroquinolone 내성기전을 조사했다. 항균제 감수성 양상을 조사하기 위해 디스크확산법이 시행되었다. Fluoroquinolone 내성과 관련된 유전자 및 돌연변이 검출을 위해 PCR과 염기서열분석이 이루어졌다. 본 연구에서 수집된 53균주의 ciprofloxacin 내성 Acinetobacter 중 47균주가 gyrA 유전자의 83번째 serine 아미노산 잔기와 parC 유전자의 80번째 serine 아미노산 잔기가 leucine 잔기로 치환된 sense mutations 가지고 있는 것으로 나타났다. gyrA와 parC 유전자에 sense mutations을 가지고 있는 47균주 중 44균주가 A. baumannii 였고 3균주는 A. pittii였다. 본 연구에서 조사대상이 되었던 Acb complex 균주들 중 plasmid-mediated quinolone resistance (PMQR) determinants를 가지고 있는 균주는 한나도 없었다. 46 균주의 ciprofloxacin 내성 A. baumannii 는 A, B, 또는 F형의 banding pattern을 보였는데 이는 충청지역에 위치한 일개의 병원에 ciprofloxacin 내성 A. baumannii가 수평확산 되어 있음을 의미한다. Fluoroquinolone 내성 Acb complex 균주의 집락화 및 확산을 막기 위해서 다제내성 균주들을 대상으로 항균제 내성인자들을 지속적으로 조사하고 모니터링할 필요가 있을 것으로 사료된다.

강원도 양식 연어과 어류에서 분리된 에로모나스 종의 유전학적 동정 (Genetic identification of Aeromonas species using a housekeeping gene, rpoD, in cultured salmonid fishes in Gangwon-Do)

  • 임종원;구본형;김광일;정현도;홍수희
    • 한국어병학회지
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    • 제30권2호
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    • pp.79-88
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    • 2017
  • 현재 양식장에서는 Aeromonad를 비롯한 다양한 병원균에 의한 전염병으로 인해 많은 경제적 손실을 겪고 있다. 연어과 어류뿐만 아니라 담수 및 해수어류에도 치명적인 감염을 야기하는 Aeromonas 종은 적어도 26종 이상이 보고되어왔으며, 전염병을 유발하는 유비쿼터스 세균이다. Aeromonas 종을 확인하기 위해 16S rDNA 및 하우스 키핑 유전자의 핵산 서열을 기반으로 한 분자생물학적 기술이 사용될 수 있다. 본 연구에서 Aeromonas 종은 강원도 16개 양식장의 연어과 어류로부터 분리되었으며 Aeromonad의 16S rDNA와 하우스 키핑 유전자의 서열, 즉 RNA polymerase sigma factor ${\sigma}^{70}$ (rpoD) 또는 DNA gyrase subunit B (gyrB)를 기반으로 계통 발생 학적으로 동정했다. 그 결과 대서양 연어 (Salmo salar), 은연어 (Oncorhynchus keta), 산천어 (Oncorhynchus masou masou), 무지개송어 (Oncorhynchus mykiss)에서 96 개의 균주가 수집되었으며, 36개의 균주가 16S rDNA 분석에 의해 Aeromonas 속으로 확인되었다. 확인된 Aeromonas 속 균주는 rpoD 또는 gyrB 유전자 서열을 기반으로 추가 분석되어 Aeromonas salmonicida (24 균주), A. sobria (10 균주), A. media (1 균주) 및 A. popoffii (1 균주)로 검출되었으며, 이 것은 Aeromonas salmonicida가 강원도의 연어과 어류에서 주요 감염균임을 나타낸다. 또 하우스 키핑 유전자의 서열에 기초한 Aeromonas 종의 계통발생학적 동정은 16S rDNA 서열보다 더 정확하다는 것이 또한 증명되었다.

Identification of sperm motility subpopulations in Gyr falcon (Falco rusticolus) ejaculate: a tool for investigating between subject variation

  • Seyedasgari, Fahimeh;Asadi, Behnam;Sebastyen, Sandor;Guillen, Roberto
    • 한국동물생명공학회지
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    • 제37권3호
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    • pp.193-201
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    • 2022
  • Subgroups of sperm which share similar motility features documented in mammals indicate between-subject variations that might be related to fertilizing potential of the respective ejaculates. The objectives of this study were to define subpopulations of motile sperm in Gyr falcon semen using kinematic parameters driven by Computer Assisted Semen Analysis (CASA) and to investigate the subject-related variations in these subpopulations. A total of 24 fresh ejaculates from 6 falcons were used to assign each of the 20473 sperms into 3 subpopulations by a multivariate cluster analysis. The proportion of sperms in different sub-populations were compared among subjects by a generalized linear model and repeatability of sperm frequency in different subpopulations was investigated by corelation analysis. The resulting 3 categories of sperm indicated significant differences in all kinematic parameters (p < 0.05). Subpopulation 1 (15.91%) contained sperms with the highest velocity and progressiveness of movement trajectory while subpopulation 3 (6.4%) included the least progressively motile sperms. Proportion of rapid and medium progressive sperm were consistently higher in the ejaculate of three falcons compared to the two other birds which also had the highest proportion of slow non-progressive sperms (p < 0.05). Respective proportion of sperms in each subpopulations indicated significant repeatability over multiple measurements (p < 0.05). In conclusion, subpopulations of motile sperm in Gyr falcon can be identified using kinematic parameters generated by CASA. Individual differences in the proportion of these subpopulations might have potential application for identifying the males with higher fertilizing capacity.

Phylogenetic Analysis of Harmful Algal Bloom (HAB)-Causing Dinoflagellates Along the Korean Coasts, Based on SSU rRNA Gene

  • Kim, Se-Hee;Kim, Keun-Yong;Kim, Chang-Hoon;Lee, Woo-Sung;Chang, Man;Lee, Jung-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제14권5호
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    • pp.959-966
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    • 2004
  • Twenty-three cultures of harmful algal bloom (HAB)-(causing dinoflagellates were isolated from the coastal waters of Korea. For each of the 14 morphospecies, the nuclearencoded small subunit (SSU) rDNA was analyzed to determine the phylogenetic relatedness of the species. Despite temporal and spatial isolation, 3-4 clonal cultures of Alexandrium catenella, Cochlodinium polykrikoides, and Gymnodinium catenatum had 100% identical SSU rDNA sequences. In contrast, heterogeneities in the SSU rDNA sequences were observed in Akashiwo sanguinea and Lingulodinium polyedrum strains. Extreme sequence polymorphism was shown within the SSU rRNA genes of an Al. tamarense clonal culture. A homology search in GenBank revealed that 11 dinoflagellate species were located in clusters corresponding to their morphological classification. The SSU rDNA sequences of C. polykrikoides, Gyrodinium instriatum, and Pheopolykrikos hartmannii, which were determined for the first time in this study, showed the following phylogenetic relationships: C. polykrikoides formed an independent branch separated from other dinoflagellates; Gyr. instriatum was placed in a monophyletic group with Gyr. dorsum and Gyr. uncatenum; and Ph. hartmanii, which forms a distinct two-celled pseudocolony, belonged to Gymnodinium sensu Hansen and Moestrup.

구상성단 M30의 UBVI CCD 측광연구 (UBVI CCD Photometry of the Globular Cluster M30)

  • 이호;전영범
    • 한국지구과학회지
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    • 제27권5호
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    • pp.557-568
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    • 2006
  • 구상성단 M30의 $20'.5{\times}20'.5$ 영역에 대한 CCD UBVI 측광 관측을 수행하여 색-등급도로부터 주계열 전향점과 색지수 $V_{TO}=18.63{\pm}0.05,\;(B-V)_{TO}=0.44{\pm}0.05,\;(V-I)_{TO}=0.63{\pm}0.05$를 얻었으며, 색-색도로부터 $E(B-V)=0.05{\pm}0.01$과 중원소 함량의 지표인 UV 색 초과량 ${\delta}(U-B)=0.27{\pm}0.01$을 얻었다. 측광학적인 방법과 분광 관측 자료를 이용하여 중원소 함량 $[Fe/H]=-2.05{\pm}0.09$를 구하였다. 관측된 M30의 광도 함수는 이론적 모델에 비하여 전향점 부근에 비하여 적색 거성열의 초과 현상을 보였다. Hipparcos 위성에서 측정된 삼각 시차로부터 거리가 알려진 준왜성을 이용하여 주계열 맞추기를 하여 거리 지수 $(m-M)_o=14.75{\pm}0.12$를 구하였다. 헬륨 함량을 구하기 위하여 R과 R' 방법을 사용하여 $Y(R)=0.23{\pm}0.02,\;Y(R')=0.29{\pm}0.02$를 얻었다. 성단의 나이는 적용하는 방법과 모델에 따라서 10.7 Gyr에서 17 Gyr까지 분산을 보인다.

Evaluation of the Diversity of Cyclodextrin-Producing Paenibacillus graminis Strains Isolated from Roots and Rhizospheres of Different Plants by Molecular Methods

  • Vollu Renata Estebanez;Fogel Rafael;Santos Silvia Cristina Cunha dos;Mota Fabio Faria da;Seldin Lucy
    • Journal of Microbiology
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    • 제44권6호
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    • pp.591-599
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    • 2006
  • To address the diversity of cyclodextrin-producing P. graminis strains isolated from wheat roots and rhizospheres of maize and sorghum sown in Australia, Brazil, and France, restriction fragment length polymorphism analysis of part of genes encoding RNA polymerase (rpoB-RFLP) and DNA gyrase subunit B (gyrB-RFLP) was used to produce genetic fingerprints. A phylogenetic tree based on rpoB gene sequences was also constructed. The isolates originated from Brazil could be separated from those from Australia and France, when data from the rpoB-based phylogenetic tree or gyrB-RFLP were considered. These analyses also allowed the separation of all P. graminis strains studied here into four clusters; one group formed by the strains GJK201 and $RSA19^T$, second group formed by the strains MC22.02 and MC04.21, third group formed by the strains TOD61, TOD 221, TOD302, and TOD111, and forth group formed by all strains isolated from plants sown in Cerrado soil, Brazil. As this last group was formed by strains isolated from sorghum and maize sown in the same soil (Cerrado) in Brazil, our results suggest that the diversity of these P. graminis strains is more affected by the soil type than the plant from where they have been isolated.