Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Mutations in the GyrA Subunit of DNA Gyrase and the ParC Subunit of Topoisomerase IV in Clinical Strains of Fluoroquinolone-Resistant Shigella in Anhui, China

  • Hu, Li-Fen (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Li, Jia-Bin (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Ye, Ying (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University) ;
  • Li, Xu (Department of Infectious Diseases, the First Affiliated Hospital of Anhui Medical University)
  • Published : 2007.04.30
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Abstract

In this research 26 Shigella isolates were examined by PCR and direct nucleotide sequencing for genetic alterations in the quinolone-resistance determining regions (QRDRs). We tested for the presence of qnr genes by PCR in 91 strains, but no qnr genes were found. The results did show, however, some novel mutations at codon 83 of gyrA ($Ser{\rightarrow}Ile$) and codon 64 of parC ($Ala64{\rightarrow}Cys,\;Ala64{\rightarrow}Asp$), which were related to fluroquinolone resistance.

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References

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