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http://dx.doi.org/10.7847/jfp.2017.30.2.079

Genetic identification of Aeromonas species using a housekeeping gene, rpoD, in cultured salmonid fishes in Gangwon-Do  

Lim, Jongwon (Department of Marine Biotechnology, Gangneung-Wonju National University)
Koo, Bonhyeong (Kangwon Fisheries disease control center)
Kim, Kwang Il (Aquaculture Industry Division, East Sea Fisheries Research Institute)
Jeong, Hyun Do (Department of Aquatic Life Medicine, Pukyong National University)
Hong, Suhee (Department of Marine Biotechnology, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.30, no.2, 2017 , pp. 79-88 More about this Journal
Abstract
At the present, fish farms are suffering a lot of economic losses due to infectious diseases caused by various pathogens including aeromonad. Aeromonad is ubiquitous bacteria that causes infectious diseases. At least 26 species in the genus Aeromonas have been reported to cause fatal infections not only in salmonid fishes, but also in other freshwater and seawater fishes. Molecular techniques based on nucleic acid sequences of 16S rDNA and housekeeping genes can be used to identify the Aeromonas species. In this study, The genus Aeromonas was isolated from salmonid fishes of sixteen fish farms in Gangwon-Do, Korea and phylogenetically identified based on the sequences of 16S rDNA and housekeeping genes for Aeromonad, i.e. RNA polymerase sigma factor ${\sigma}^{70}$ (rpoD) or DNA gyrase subunit B (gyrB). Consequently, 96 strains were collected from Atlantic salmon (Salmo salar), coho salmon (Oncorhynchus kisutch), masou salmon (Oncorhynchus masou) and rainbow trout (Oncorhynchus mykiss), and 36 isolates were identified as the genus Aeromonas by 16S rDNA analysis. Thirty six Aeromonad isolates were further analysed based on rpoD or gyrB gene sequences and found Aeromonas salmonicida (24 isolates), A. sobria (10 isolates), A. media (1 isolates) and A. popoffii (1 isolates), indicating that A. salmonicida is a main infectious bacteria in Salmonid fishes in Gangwon-Do. It was also proved that the phylogenetic identification of Aeromonas species based on the sequences of housekeeping gene is more precise than the 16S rDNA sequence.
Keywords
Aeromonas; Salmonid; Identification; rpoD; 16S rDNA;
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