• Title/Summary/Keyword: guinea pig

Search Result 512, Processing Time 0.033 seconds

Cholinergic Effects of Nizatidine on the Guinea Pig Ileum (Mizatidine의 기니픽 회장에서의 Choline성 작용)

  • Chang, Woo Sung;Chung, Jae Kyung;Kim, Chang Kyun;Jung, Kuk Hyun;Lee, Seok Yong;Cho, Tae Soon
    • Korean Journal of Clinical Pharmacy
    • /
    • v.8 no.2
    • /
    • pp.113-121
    • /
    • 1998
  • To clarify whether nizatidine, a $H_2$ receptor antagonist, has the cholinergic activity, the effects of nizatidine on the guinea pig ileum and on the acetylcholinesterase in human serum were studied. And, the mechanism of excitatory effect of nizatidine on the cholinergic system in ileum was also studied. Nizatidine caused a concentration-dependent contractile response by the guinea pig ileum. The $EC_{50}\;was\;53\;{\mu}M$ and the maximum response was at $300\;{\mu}M$. Ranitidine also caused a contractile response by the guinea pig ileum, but cimetidine and famotidine did not. The pretreatment with $H_1$ receptor antagonist did not affect the actions of nizatidine on the guinea pig ileum, but the pretreatment with atropine completely blocked them. Nizatidine significantly enhanced the acetylcholine-induced response of the guinea pig ileum, but not the pilocarpine-induced response. Nizatidine did not affect the histamine-induced response of the guinea pig ileum. Nizatidine still exerted the small excitatory effect on the guinea pig ileum pretreated with the high concentration of physostigmine. Nizatidine significantly inhibited the acetylcholinesterase in human serum. These results suggest that nizatidine exerts an excitatory effect on guinea pig ileum which seems to be associated with the cholinergic system, probably through an indirect mechanism, inhibition of acetylcholinesterase and/or increased release of acetylcholine.

  • PDF

Effects of Jasoeumja Extract On the Contraction of Isolated Guinea pig Trachea Smooth Muscle (자소음자(紫蘇飮子)가 GUINEA PIG의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響))

  • Song, Jin-Oh;Han, Sang-Whan
    • The Journal of Internal Korean Medicine
    • /
    • v.11 no.1
    • /
    • pp.77-91
    • /
    • 1990
  • This study was carried out to investigate the effect of Jasoeumja extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows: 1. The isolated trachea smooth muscle was suspended in the Organ bath with oxygenated Kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer (Nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Jasoeumja extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by Jasoeumja extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was considerably inhibited by Jasoeumja extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydroxytryptamine $10^{-4}M$ was considerably inhibited by Jasoeumja extract.

  • PDF

Effects of Haepyoyangjintang and Haepyoejintang Extract On the Contraction of Isolated guinea pig trachea smooth muscle (해표양진탕(解表兩陳湯) 및 해표이진탕(解表二陳湯)이 Guinea Pig의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響))

  • Park, Cheon-Su;Han, Sang-Hwan
    • The Journal of Internal Korean Medicine
    • /
    • v.11 no.2
    • /
    • pp.68-79
    • /
    • 1990
  • This study was carried out to investigate the effect of Heapyoyangjintang and Haepyoejintang extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows : 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer(nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Haepyoyangjintang and Haepyoejintang extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine$10^4M$ Was significantly inhibited by Heapyoyangjintang and Haepyoejintang extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine$10^4M$ was considerably inhibited by Haepyoyangjintang and Haepyoejintang extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydorxytrtrypptamine $10^4M$ was considerably inhibited by Haepyoyangjintang and haepyoejintang extract.

  • PDF

Effects of Samyootang and Shinchulsan Extract on the Contraction of Isolated Guinea pig's Trachea Smooth Muscle (삼요탕(三拗湯) 및 신출산(神朮散)이 GUINEA PIG의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響))

  • Oh, Young-Oug
    • The Journal of Internal Korean Medicine
    • /
    • v.13 no.2
    • /
    • pp.84-94
    • /
    • 1992
  • This study was carried out to investigate the effect of samyootang and shinchulsan extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate it's mechanism The isolated were obtained as follows ; 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recored with Isometric transducer (nacro F-60) The resting tension was approximately 0.5g 2. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was considerably inhibited by samyootang and shinchulsan extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine $10^{-4}M$ was significantly inhibited by samyootang and shinchulsan extract.

  • PDF

Effect of Chunggeumeum Extract on The Contraction of Isolated Guinea Pig Trachea Smooth Muscle (청금음(淸金飮)이 GUINEA PIG의 기관지(氣管支) 평골근(平滑筋)에 미치는 영향(影響))

  • Lee, Cheol-Hyeon;Han, Sang-Whan
    • The Journal of Internal Korean Medicine
    • /
    • v.16 no.1
    • /
    • pp.146-159
    • /
    • 1995
  • These studies were carried out to investigate the effect of Chunggeumeum water extract on the inhibitory contractile action of acetylcholine in guinea pig. The results of these studies were as follows; 1. Contractile force of acetylcholine from trachea smooth muscle in guinea pig was significantly inhibited by Chunggeumeum. 2. Dose-response of acetylcholine pretreated Chunggeumeum in guina pig was not significantly changed. 3. Inhibitory contractile action of acetylcholine pretreated propranolol in guinea pig was decreased. 4. Inhibitory contractile action of acetylcholine pretreated indomethacin was not significantly changed by Chunggeumeum. 5. Inhibitory contractile action of acetylcholine pretreated methylene blue was not significantly changed by Chunggeumeum.

  • PDF

Comparison of Skin Response Between New Zealand White Rabbit and Hartley Guinea Pig to Glycolic Acid (Glycolic Acid에 대한 토끼와 기니픽간의 피부자극성 비교)

  • 안광수;정경미;김용규;이종권;박기숙;김은정;이선희;홍진태
    • Toxicological Research
    • /
    • v.17 no.1
    • /
    • pp.59-63
    • /
    • 2001
  • In this study, we compared skin response between Hartley guinea pig and New Zealand white rabbit. New Zealand white rabbit was treated by a glycolic acid (0, 8, 24, 40, 56 mg/$cm^2$) and UVB (0, 0.4, 3.0 J/$cm^2$) for 14 days. Skin irritation by glycolic acid and UVB were increased in dose and time-dependent manners, and the combination treatment of UVB increased glycolic acid-induced skin irritation. Comparison the skin irritation index between guinea pig and rabbit showed that guinea pig was much more sensitive to glycolic acid and UVB. This study indicated that selection of reliable species of animal could be considered in chemical-induced skin irritation study.

  • PDF

Effects of Banhaonpaetang(半夏溫肺湯) extract and its Constituent herbs on the Contraction of Isolated Guinea Pig Trachea Smooth Muscle (반하온폐탕(半夏溫肺湯) 및 그 구성약물(構成藥物)이 실험(實驗) 동물(動物)에 미치는 영향(影響))

  • Song, Jin-Oh;Han, Sang-Hwan
    • The Journal of Internal Korean Medicine
    • /
    • v.13 no.2
    • /
    • pp.1-15
    • /
    • 1992
  • This study has been carried out to investigate the effects of Banhaonpaetang extract and its constituent herbs on the contractile force of Isolated Guinea pig trachea smooth muscle and to elucidate its mechanism. The results were as follows; 1. Banhaonpaetang significanly inhibitd the contractile force of isolated guinea pig trachea smooth muscle pretreated by histamine. 2. Banhaonpaetang significantly inhibited the contractile force of isolated guinea pig trachea smooth muscle pretreated by acetylcholine. 3. Radix Asari(細辛), Flos Inulae(旋覆花), Pericarpium Citri Nobilis(陳皮) and Radix Ginseng(人蔘) extract significantly inhibited the contractile force of isolated guinea pig trachea smooth muscle pretreated by histamine. 4. Radix Asari(細辛), Cortex Cinnamomi(桂心), Flos Inulae(旋覆花), Pericarpium Citri Nobilis(陳皮) and Radix Ginseng(人蔘) extract significantly inhibited the contractile force of isolated guinea pig trachea smooth muscle pretreated by acetylcholine. 5. The effects of Banhaonpaetang extract on the changes of plasma ACTH in rats were shown to be insignificant. 6. The effects of Banhaonpaetang extract on the changes of serum electrolytes were shown to be significant in $K^+$ and $Ca^{2+}$.

  • PDF

Studies on the Effects of Haengsoyeum (杏蘇飮) Water Extract on the Plasma Cortisol Concentration in the Rabbit and the Contraction of Isolated Guinea Pig Tracheal Smooth Muscle (행소음전탕액(杏蘇飮煎湯液)이 가토혈장(家兎血漿) COTISOL농도(濃度) 및 $PCO_2,\;CO_2$와 GUINEA PIG의 기관지(氣管支) 평활근(平滑筋)에 미치는 영향(影響))

  • Lee, Sueng-Ho
    • The Journal of Internal Korean Medicine
    • /
    • v.13 no.1
    • /
    • pp.143-155
    • /
    • 1992
  • The following results were obtained from the observation on the change of plasma cortisol concentration in the experiment of intravenous administration of Haengsoyeum Water Extract in the rabbit. And the effects of Haengsoyeum extract on the contractile force of the isolated guinea pig trachea smooth muscle. 1. In intravenous administration the plasma cortisol concentration increased significantly about 1 hours after with a does of 0.2 ml/kg. 2. In intravenous administration the plasma cortisol concentration increased significantly about from 1 to 3 hours after with a does of 0.4 ml/kg. 3. The contractile response of the trachea smooth muscle of isolated guinea pig to histamine $10^{-4}\;M$ was significantly inhibited by Haengsoyeum extract. 4. The contractile response of the trachea smooth muscle of isolated guinea pig to acetylcholine $10^{-4}\;M$ was considerably inhibited by Haengsoyeum extract. 5. The contractile response of the trachea smooth muscle of isolated guinea pig to 5-hydroxytryptamine $10^{-4}\;M$ was inhibited by Haengsoyeum extract.

  • PDF

Bronchodilator Effect of Higenamine in Isolated Guinea-pig Tracheal Smooth Muscle (Higenamine의 Guinea pig 기관 평활근 이완작용)

  • Yun, Hyo-in;Chang, Ki-chorl;Hong, Seong-geun;Lee, Chang-eop
    • Korean Journal of Veterinary Research
    • /
    • v.27 no.1
    • /
    • pp.35-40
    • /
    • 1987
  • Higenamine, a benzyltetrahydroisopuinoline analog isolated from aconite tuber, has potent isotropic action. Recent studies suggest it may have beta receptor agonistic property in that its inotropic action is blocked by propranolol in isolated rabbit heart. However, no study has been carried out on other organs than heart. Higenamine is expected to have pharmacological actions on smooth muscle on the ground that it has catecholamine moiety and tetrahydrosioquinoline nucleus in its chemical structure, both of which are well known to have smooth muscle relaxation effects. Therefore present study was aimed at determining whether higenamine has bronchodilating effect in isolated guinea pig trachea smooth muscle rich in adrenergic beta receptor and if any, it has agonistic effect on beta receptor. The results were summarized as follows : 1. Higenamine had remarkable bronchodilating effect in guinea pig tracheal smooth muscle in a dose-dependent manner. 2. Bronchodilator effect of higenamine in isolated guinea pig tracheal smooth muscle was blocked competitively by propranolol. The $pD_2$ value of higenamine in isolated guinea pig tracheal smooth muscle was 5.65 and the $pA_2$ value of propranolol against higenamine in the same preparation was 7.97.

  • PDF

Effects of Aqueous Extract from Aconitum Koreanum on the Expression of Tyrosinase-related Proteins by Ultraviolet B Irradiation in Guinea Pig Skin (백부자의 추출물이 자외선 B조사에 의한 기니피그 피부의 tyrosinase-related proteins발현에 미치는 영향)

  • Lee, Sang-Bok;Park, Dong-Il;Kim, Hoon;Gil, Young-Gi;Choi, Byung-Tae
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.22 no.2
    • /
    • pp.346-349
    • /
    • 2008
  • To investigate whether aqueous extract from Aconitum koreanum (AEAK) effects in the process of melanin synthesis, the expression of tyrosinase-related proteins (TRPs) by immunohistochemical methods were performed in ultraviolet B (UVB) irradiated skin of guinea pig. The irradiation of UVB (60 mJ/day) was performed for 3 days and treated with AEAK for 15 days. About the color evaluation, the visual scores of UV B irradiated guinea pig with AEAK treatment were slightly lower than those in the UV B alone irradiated ones. At day 15 after UVB exposure, immunohistochemical analysis for TRPs expression were performed. The intensive expression of tyrosinase was mainly observed over epidermis with skin appendage and in the cells of dermis. Slight increase of these reaction was induced in response to UVB in the spinous and granular layer of epidermis, but similar expression in the AEAK treated guinea pig as normal one. The TRP-1 and TRP-2 expression were not detected in the skin of normal guinea pig. But intensive expression for TRP-1 and TRP-2, especially TRP-2, induced by UV B irradiation in the cells of dermis. These expressions were decreased in the AEAK treated guniea pig. Collectively, these results suggest that AEAK has a potential to inhibit synthesis through regulation of TRPs expression in the skin of guinea pig, but better understanding the function of AEAK, more research should be done in the effects of AEAK on the function of TRPs in melanogesis.