• Molecules and Cells
• /
• 제46권10호
• /
• pp.637-653
• /
• 2023

The physiology of most organisms, including Drosophila, is heavily influenced by their interactions with certain types of commensal bacteria. Acetobacter and Lactobacillus, two of the most representative Drosophila commensal bacteria, have stimulatory effects on host larval development and growth. However, how these effects are related to host immune activity remains largely unknown. Here, we show that the Drosophila development-promoting effects of commensal bacteria are suppressed by host immune activity. Mono-association of germ-free Drosophila larvae with Acetobacter pomorum stimulated larval development, which was accelerated when host immune deficiency (IMD) pathway genes were mutated. This phenomenon was not observed in the case of mono-association with Lactobacillus plantarum. Moreover, the mutation of Toll pathway, which constitutes the other branch of the Drosophila immune pathway, did not accelerate A. pomorum-stimulated larval development. The mechanism of action of the IMD pathway-dependent effects of A. pomorum did not appear to involve previously known host mechanisms and bacterial metabolites such as gut peptidase expression, acetic acid, and thiamine, but appeared to involve larval serum proteins. These findings may shed light on the interaction between the beneficial effects of commensal bacteria and host immune activity.

• 성인간호학회지
• /
• 제17권1호
• /
• pp.88-99
• /
• 2005

Objective: The purpose of this study were to: (1) describe the individual characteristics, perceived health status, body image, and health promoting behaviors of staff nurses working in a metropolitan hospital and (2) determine the relationship of perceived health status, body image, and health promoting behaviors. Methods: Data were collected from 311 staff nurses working at one of university affiliated hospitals using a self-administered questionnaire for perceived health status(SF 36 V2), body image(BAQ), and health promoting behaviors (HPLP-II) and using the report of year 2002 employee physical check-up results for health related characteristics. Results: Most of nurses were within the normal range of BMI, total cholesterol, and liver enzymes but 42.2% had systolic BP above 120mmHg and 37.3% hemoglobin below 12g/dL. Although 96.4% of BMI score indicated 'underweight' or 'normal', 'feeling fat' showed the highest. Among health promoting behaviors the most frequently reported one was spiritual growth and the least one was engagement in physical activity. In the correlational analysis, health promoting behaviors had the positive relationships with perceived health status, vitality, mental health, attractiveness, strength and fitness (p=.000 - .004). Conclusion: These findings provide information that is relevant in designing interventions to enhance health promoting behaviors among nurses working in a hospital.

• 한방안이비인후피부과학회지
• /
• 제15권2호
• /
• pp.53-79
• /
• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth promoting effect was measured using hair growth index. As a result, Prunus mume, black bean, Brassica campestris subsp. black sesame and Rubi Fructus showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Prunus mume, Eriobotryae Folium showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of the effect of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT - PCR analyses were performed. However, there were no plant extracts, which have profound effect on the gene expression of several growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another tests for inhibition of microbial such as P. acne were also carried out to find whether these plant extracts have anti -microbial activities. Rubi Fructus showed anti -microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 대한한의학회지
• /
• 제41권4호
• /
• pp.27-40
• /
• 2020

Objectives: This study was conducted to evaluate the hair growth-promoting effects by Rumex japonicas Houttuyn ethanol extract (RJHEE) in C57BL/6N mice and HaCaT cells. Methods: The hair growth effect was examined by topical application of RJHEE on the shaved dorsal skin of C57BL/6 mice. Six-week old mice were depilated and separated in 4 groups; CON (vehicle treatment), MXD (2% Minoxidil), and RJHEE (2% and 4%). The treatments were applied daily for 17 days. The hair growth was determined photographically and the hair density, thickness and length were identified by Folliscope. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blotting. In HaCaT cells, the cell proliferation and the protection against H₂O₂-induced cell damage by RJHEE were analyzed. Results: Our results indicate that RJHEE promote the hair growth, hair density, thickness and length. RHE activate the Wnt/𝛽-catenin signaling and induced the expression of cell survival-related proteins, such as pERK/ERK and Bcl-2/Bax. In HaCaT, RJHEE accelerated the cell proliferation and protected the H₂O₂-induced cell damage. Conclusions: Our results strongly suggest that RJHEE promotes hair growth by regulating the activation of Wnt/𝛽-catenin signaling and cell survival signaling and protects oxidative stress-induced hair damage. Therefore, RJHEE has a hair growth activity and can be useful for the treatment of alopecia.

• 여성건강간호학회지
• /
• 제17권4호
• /
• pp.337-345
• /
• 2011

Purpose: The purpose of this study is to examine the impact of type D personality on health status and health promoting behaviors in middle-aged women. Methods: A cross-sectional study design was used with 220 middle aged women. All participants completed 3 measuring tools: a 14-item Type D Personality Scale, a 12-item Short Form Health Survey Questionnaire version 2, and Health Promoting Lifestyle Profile-version II. All were completed in November 2010. Results: The prevalence of type D personality was 34.5%. Type D women had significantly lower physical (p=.020) and mental health status (p<.001) compared with non-type D women. In addition Type D women reported significantly poorer performance of health responsibility (p=.015), physical activity (p=.001), nutrition (p=.027), spiritual growth (p<.001), interpersonal relations (p<.001) and stress management (p<.001) techniques in health behaviors than non-type D women. Conclusion: Type D personality is a vulnerability factor that affects health status and is associated with poor health promoting behaviors in middle aged women. Therefore, screening for Type D personality is important to detect women at risk for health status and quality of life in community settings in Korea.

• KSBB Journal
• /
• 제24권4호
• /
• pp.334-340
• /
• 2009

식물성장 촉진 기능을 가지는 PGPR (plant growth-promoting rhizobacteria)균은 특수한 토양시료를 사용하여 내열성을 가지는 400개의 균주를 분리하였다. 이들 분리균주에서 선발한 공시균주 Bacillus sp. YK-5는 항진균성 물질을 생산하였으며, 시험균주 Asp. flavus를 대상으로 생산조건을 검토한 결과, 공시균주의 생육배지는 생육촉진효과와 식물병원균에 대한 방제를 위해 1% peptone 및 yeast extract, 5% black sugar가 함유된 modified LB배지를 사용하였다. 식물병원균에 대한 공시균주의 길항효과는 시험균주인 식물병원균 Fusarium oxysporum KACC 40052균에 대해 배양 7일째까지 강한 생육저해를 보였다. 공시균의 식물성장촉진능은 궁중무 및 벼를 대상으로 pot 실험한 결과, 궁중무의 경우 경엽수, 줄기의 길이 및 뿌리의 길이가 무처리구에 비해 약 60%이상 우수한 성장촉진효과를 나타내었으며, 벼의 경우 이식 후 시비 8일째 시판제품 처리구에 비해 공시균주 처리구는 잎의 길이와 흰 세근의 발달이 우수하여 뿌리의 발육에 현저한 생육촉진 효과를 나타내었다. 공시균주의 동정은 분자계통분류학적 분석 (형태, 배양, 생리, 화학적 및 분자생태학적 측면)에 의해 B. subtilis YK-5로 명명하였다.

• 한국토양비료학회지
• /
• 제43권1호
• /
• pp.96-104
• /
• 2010

무균 및 온실조건에서 indole-3-acetic acid (IAA)의 처리와 1-aminocyclopropane-1-carboxylate deaminase (ACCD) 및 IAA 활성을 갖는 Methylobacterium 균주 접종 시 토마토와 고추의 생장을 비교 평가하였다. 무균조건에서 1.0 ${\mu}g\;mL^{-1}$의 IAA는 고추와 토마토의 뿌리생장을 촉진시키는데 비해 10.0 ${\mu}g\;mL^{-1}$ 이상의 높은 농도에서는 뿌리생장이 억제되었다. 그러나 높은 ACCD 활성을 갖고, IAA 활성은 낮거나 가지고 있지 않은 Methylobacterium 균주들을 접종하였을 때에는 고추와 토마토 모두 IAA 처리구 보다 뿌리생장이 증진되는 것을 확인하였다. 마찬가지로 온실조건에서 Methylobacterium 균주들을 접종했을 때, 마디길이와 잎의 크기 그리고 단위 면적당 잎의 무게 (SLW)에서 유의성 있는 증진효과를 보였다. 전반적인 식물 생장에서 저농도의 IAA 처리 효과는 Methylobacterium의 효과와 비슷한 경향을 나타냈다. 유묘의 지상부 길이는 ACCD 활성과 IAA 생산능을 갖는 Methylobacterium 균주 처리구에서 유의성 있는 증가를 확인할 수 있었으며, 전체 건물중 또한 Methylobacterium 처리 시 유의성 있는 증진 효과를 확인 할 수 있었다. 하지만 고농도의 IAA는 고추와 토마토의 생물량을 억제시켰다. 이러한 결과는 접종 균주의 IAA와 ACCD가 고추와 토마토 유묘의 생장 증진에 영향을 끼친다는 것을 증명한다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제7권2호
• /
• pp.91-96
• /
• 2003

In Korean folk medicine, several herbs, Glycyrrhizae Radix, Persicae Semen, Salviae Radix, Angelicae Gigantis Radix, Zanthoxyli Fructus, Ginseng Radix Alba, Cnidii Rhizoma, and Carthami Flos, are known to enhance blood circulation and have wound healing or anti-inflammatory effects. These pharmacological actions prompted us to investigate whether these herbs might stimulate hair growth. Thus, using a mixture of their extracts called SPELA 707, we investigated their effects and found that SPELA 707 possessed significant hair cycle converting activity from the telogen phase to the anagen phase in C3H mice. Furthermore, we found that SPELA 707 enhanced the hair density in subjects with hair loss and also promoted the conversion of hair into the anagen phase in subjects with androgenetic alopecia. In addition, hair growth promotion effect of SPELA 707 occurred through inhibition of steroid $5{\alpha}$-reductase activity, which is known to block hair growth. Taken together, these results suggest that SPELA 707 has a potential to be used for the treatment of hair loss.

• 한국미생물·생명공학회지
• /
• 제20권3호
• /
• pp.249-256
• /
• 1992

국내에서 구입 가능한 주요 식이 소재로부터 장내의 대표적인 유해균인 Clostridium perfringens의 생육을 억제시키는 소재를 탐색한 결과 감자즙에서 강력한 항균활성을 발견하였다. 감자즙 중 70% 황산암모늄용액에서 침전되는 단백질성 물질이 항균활성을 나타낸 것으로 밝혀졌으며, 이 감자단백질은 pH 4~10 사이에서는 안정하나 $60^{\circ}C$에서 10분간의 열처리로 그 활성이 소실되었다.

• 한방안이비인후피부과학회지
• /
• 제29권3호
• /
• pp.74-94
• /
• 2016

Objectives : YangHyulEum Gami-Bang(YHEG) is a hair care extracts which is composed of fourteen plant extracts used in oriental medicine. The purpose of this study is to investigate the effect of YangHyulEum Gami-Bang(YHEG) on the alopecia and hair growth.Methods & Results : The herbal extracts from YangHyulEum Gami-Bang(YHEG) was tested using in vivo and in vitro test models. 1. The YHEG extracts showed effect on the DNA proliferation of the hair dermal papilla cells measured by [3H]thymidine incorporation. 2. YHEG showed promoting on the expression of growth factors such as IGF-1, KGF-1 and inhibiting on the expression of inhibitory hair growth factor such as TGF-β1, BMP-2 estimated by qPCR. 3. The YHEG extracts showed effect on the activation of β-catenin in the dermal papilla cells. 4. YHEG showed inhibitory effects of NO synthesis at 0.2% concentrations. 5. YHEG showed effects in the expression of IL-1β, TNF-α, IL-6, COX-2 and iNOS gene in the LPS stimulated RAW 264.7 cells. 6. The hair growth index of the YHEG extracts ranked at over 2 when compared to control group which was ranked at 0. 7. The hair follicle number, length and size of the experimental group were remarkably higher than the control group in the histological observation.Conclusions : These results suggest that YangHyulEum Gami-Bang(YHEG) has hair growth promoting activity and it can be used as a potent treatment agent for preventing hair loss and stimulating hair growth for treatment of alopecia.