• 한국잡초학회지
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• 제17권3호
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• pp.325-333
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• 1997

ALS저해형 제초제 imazethapyr와 primisulfuron에 내성인 고추품종을 선발하기 위하여 42종 고추품종을 공시하여 고추의 약해, 초장, 생체중 및 건물중을 측정하여 품종 반응을 조사하였다. 1. 레드탑, 해피드라이, 골든 타워, 하교레 품종은 imazethapyr에 내성을, 코리아, 조양, 오리엔탈 글로리, 하남 품종은 감수성을 나타냈다. 레드혼, 조풍, 광복, 왕조는 primisulfuron에 내성을, 코리아, 다홍, 참조아, 풍천 품종은 감수성을 나타냈다. 2. Imazethapyr에 대한 $I_{50}$ 농도는 감수성 품종은 0.075kg/ha, 내성품종은 0.20kg/ha이며, primisulfuron의 경우, 감수성 품종은 0.065kg/ha, 내성품종은 0.16kg/ha이었다. 3. Imazethapyr 처리에 따른 $GR_{50}$을 감수성 품종에서 0.05kg/ha, 내쟁품종에서 0.20kg/ha이며 primisulfuron 처리의 경우 감수성 품종은 0.07kg/ha, 내성품종은 0.16kg/ha이었다. 4. 따라서 ALS저해형 제초제에 대한 내수성품종과 감수성 품종간 내성 정도는 imazethapyr에서 3~4배, primisulfuron에서는 2~3배 차이를 나타냈다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.330-331
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• 2010

• 한국작물학회:학술대회논문집
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• 한국작물학회 1995년도 춘계 학술대회지
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• pp.94-95
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• 1995

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1286-1289
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• 2008

The ability of Lactococcus strains to inhibit the growth of intestinal bacteria was examined. In in vitro cocultures, we observed that among eighteen Lactococcus strains tested, the ability to inhibit growth of Escherichia coli varied, with the L. lactis N7 showing the greatest growth inhibition. Strain N7 ($8.94\times10^{10}$ CFU/day for 7 days) was orally administered to mice, and the viable count of strain N7 in feces appeared at a level of $10^{4-5}$ CFU/g. After administration, the proportion of Bacteroidaceae to total intestinal bacteria decreased. Lactococci may act as probiotic bacteria by inhibiting the growth of harmful bacteria.

• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1507-1513
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• 2015

Pemetrexed is an antifolate agent which has been used for treating malignant pleural mesothelioma and non small lung cancer in the clinic as a chemotherapeutic agent. In this study, pemetrexed inhibited cell growth and induced G1 phase arrest in the A549 cell line. To explore the molecular mechanisms of pemetrexed involved in cell growth, we used a two-dimensional polyacrylamide gel electrophoresis (2-DE) proteomics approach to analyze proteins changed in A549 cells treated with pemetrexed. As a result, twenty differentially expressed proteins were identified by ESI-Q-TOF MS/MS analysis in A549 cells incubated with pemetrexed compared with non-treated A549 cells. Three key proteins (GAPDH, HSPB1 and EIF4E) changed in pemetrexed treated A549 cells were validated by Western blotting. Accumulation of GAPDH and decrease of HSPB1 and EIF4E which induce apoptosis through inhibiting phosphorylation of Akt were noted. Expression of p-Akt in A549 cells treated with pemetrexed was reduced. Thus, pemetrexed induced apoptosis in A549 cells through inhibiting the Akt pathway.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.885-893
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• 2001

This review summarizes some recent research into ways of improving the productivity of ruminal fermentation by increasing protein flow from the rumen and decreasing the breakdown of protein that results from the action of ruminal microorganisms. Proteinases derived from the plant seem to be of importance to the overall process of proteolysis in grazing animals. Thus, altering the expression of proteinases in grasses may be a way of improving their nutritive value for ruminants. Inhibiting rumen microbial activity in ammonia formation remains an important objective: new ways of inhibiting peptide and amino acid breakdown are described. Rumen protozoa cause much of the bacterial protein turnover which occurs in the rumen. The major impact of defaunation on N recycling in the sheep rumen is described. Alternatively, if the efficiency of microbial protein synthesis can be increased by judicious addition of certain individual amino acids, protein flow from ruminal fermentation may be increased. Proline may be a key amino acid for non-cellulolytic bacteria, while phenylalanine is important for cellulolytic species. Inhibiting rumen wall tissue breakdown appears to be an important mechanism by which the antibiotic, flavomycin, improves N retention in ruminants. A role for Fusobacterium necrophorum seems likely, and alternative methods for its regulation are required, since growth-promoting antibiotics will soon be banned in many countries.

• Natural Product Sciences
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• 제19권4호
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• pp.297-302
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• 2013

The current study was conducted to evaluate the antibacterial and antioxidant activities of the essential oil fraction from the roots of Angelica tenuissima Nakai and its main components. We extracted the essential oil fraction from the roots of A. tenuissima using steam distillation and isolated its main components. Their antibacterial activities were determined by broth dilution test against food-borne pathogenic bacteria. Antioxidant activities were evaluated by DPPH-scavenging assay and reducing-power test. Also tested was their ability to inhibit the growth of two gastrointestinal cancer cell lines, Caco-2 and MKN-45. The A. tenuissima oil fraction and its main components, ligustilide and butylidene phthalide exhibited marked inhibitory effects against most of the tested antibiotic-susceptible and antibiotic-resistant bacterial strains with minimum inhibiting concentrations (MICs) from $0.21{\pm}0.08$ to $3.60{\pm}0.89mg/ml$. They also showed growth-inhibiting activity against Caco-2 and MKN-45 cells. The oil fraction showed significant antioxidant activities in DPPH radical scavenging assay and reducing-power test. Taken together, A. tenuissima essential oil could be used as a safe additive for preventing food contamination by pathogenic bacteria. Additionally, its antioxidative activity and the ability to inhibit gastrointestinal carcinoma cell lines could increase its value for functional foods and prevention of cancer.

• 혜화의학회지
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• 제10권2호
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• pp.21-29
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• 2002

To evaluate the antitumor activity and antimetastatic effects of Polygoni Orientalis Fructus(POF), studies were done experimentally. The results were obtained as follows : 1. In cytotoxicity against A549, and XF498 cell concentration inhibiting cell growth up to below 30% of control was recognized at $200{\mu}g/m{\ell}$ of POF. Also POF inhibited cell growth up to below 30% of control against SK-OV-3, SK-MEL-2, HCT15 and MCF-7 cell at $100{\mu}g/m{\ell}$. 2. The concentration inhibiting adhesion of A549 and SK-OV-3 to complex extracellular matrix up to below 70% of control was recognized at $100{\mu}g/m{\ell}$ of POF. 3. In pumonary colonization assay, a number of colonies in the lungs were decreased significantly in POF treated group as compared with control group. These results suggested that POF extracts might be usefully applied for prevention and treatment of cancer.

• 혜화의학회지
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• 제8권1호
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• pp.131-146
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• 1999

To evaluate the antitumor activity of Kamisagoonjatang(KST), Kami-jihwangtang (KJT) and Kamigoonjajihwangtang(KKJT), studies were done experimentally. The results were obtained as follows: 1. In cytotoxicity against B16-F10, HT1080, SNU, and L1210, con-centration inhibiting cell growth up to below 50% of control was recognized at $10^{-3}g/ml$ of KKJT. 2. In cytotoxicity against A549, SK-OV-3, XF498 and HCT15, concentration inhibiting cell growth up to below 30% of control was over $400{\mu}g/ml$ of KKJT only and also over $200{\mu}g/ml$ against SK-MEL-2. 3. In Inhibitory effect on activity of DNA topoisomerase I, the $IC_{50}$ was shown $200-400{\mu}g/m{\ell}$, of KST, over $400{\mu}g/m{\ell}$ of KJT and $100-200{\mu}g/m{\ell}$ of KKJT. 4. The T/C% was 122.8 in KJT, 127.4 in KST and 158.4 in KKJ-Ttreated group in S-180 bearing ICR mice. 5. In hematological changes in S-180 bearing ICR mice, numbers of WBC were decreased significantly in KJT and KKJT treated groups as compared with control, whereas those of platelet were increased with no significance in all groups as compared with control. From above results it was concluded that KKJT could be usefully applied for the prevention and treatment of cancer.

• KSBB Journal
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• 제18권1호
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• pp.45-50
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• 2003

송이의 공시균(KFR1432)과 실험실에서 자체 분리한 균(YxT, WxT, $\textrm{NK}_3\textrm{T}$)의 생장속도의 차이와 FE SEM으로 확인한 미세구조의 차이로 공시균과 자체 분리균이 서로 다른 균임을 확인할 수 있었다. Tyrosinase 활성 억제 실험을 위해 각각 균사체의 열수 추출물, 에탄올 추출물, 배양액을 hexane, chloroform, ethyl acetate로 분획하였다. 그 결과 모든 균의 ethyl acetate 분획층과 수층에서 tyrosinase 활성을 억제하는 것으로 나타났으며, KFR1432의 ethyl acetate 분획층과 YxT의 수층에서 억제율이 100%를 넘어 큰 저해 활성을 보였다. 또한 항산화제 처리 결과 ascorbic acid와 glutathion은 생장을 다소 증가시키거나 별 영향을 주지 못한 반면, tocopherol 의 경우 농도가 높아질수록 생장을 억제하는 것으로 나타났다. Tyrosinase 활성 억제에 있어서는 모든 항산화제 처리구에서 무처리구에 비해 높은 억제율을 보였다.