• 한국식품영양과학회지
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• 제28권2호
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• pp.458-463
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• 1999

Methanol extract and its fraction of traditional fermented soybean paste(doenjang) from Soonchang area were studied for growth suppression on the various tumor cells and suppression components, by using HPLC and GC were analysed. Hexane fraction of methanol extract was indicated 79%, 76%, 67%, 66%, 78% of growth suppression on L1210, P338D1, HepG2, WiDr and SNU 1 tumor cells, respectively. Ethylacetate fraction of methanol extract also showed 81%, 75%, 75%, 76% and 82% of growth suppression on the same tumor cells, respectively. Peak 8 obtained from HPLC of ethylacetate fraction indicated 81%, 77%, 77%, 75% and 79% of growth suppression on the same tumor cells and identified as a genistein, by comparing with standard one by HPLC analysis. Hexane fraction of methanol extract contained oleic acid, linoleic acid and palmitic acid.

• 한국식품영양학회지
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• 제12권5호
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• pp.470-477
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• 1999

The purpose of this study was to choose prinicipal food components contained in diet foods and food additives used for manufacturing processed foods and elucidate their in vivo effects on the growth pattern of Helicobacter pylori. To do this the antibacterial effects of various sources of carbon nitrogen and mineral as an effect agent on Helicobacter pylori were first assessed based upon bacterial growth degree. results show that the source of carbon tested had different effects on bacterial growth of Helicobacter pylori. It was revealed that a promotional effect of monosaccharides resulted in enhanced growth of Helicobacter pylori compared with disaccharides and polysacchrides, in particular glucose was observed to be most effective in growth of Helicobacter pylori among monosaccharides teste whereas mannose to hinder the growth of Helicobacter pylori. Polyols such as sorbitol mannitol maltitol and xylitol was however observed to show no promotion or suppression effect on growth of Helicobacter pylori. Apart from these the sources of amino acid and inorganic nitrogen were chosen and tested to assess the promotion or suppression effect of nitrogen sources on growth of helicobacter pylori. It was found that amino acid such as lysine showed its promotion effect on the growth of Helicobacter pylori while arginine (NH4)2SO4 and NH4Cl showed no effect on its growth. Ammoia and urea were however observed to have a positive effect on the growth of Helicobacter pylori. Among these effect agents lysine and methionine were revealed to show the most positive effect on growth of Helicobacter pylori. Minerals such as MgSO4 KH2PO4 and MgCl2 appered to exert their positive growth effects whereas CaCl2 and CaSo4 had a little effect. In addition FeSO4 FeCl2 and FeCl3 brought suppression on the growth of helicobacter pylori. In studies of the growth of Helicobacter pylori by food additives ascorbic acid showed extreme suppression on its growth,. Sodium nitrate and sodium chloride were also found to be of negative effect on the growth of Helicobacter pylori in rder of degree whereas tocopherol had nothing to do with microbial growth.

• 한국콘텐츠학회논문지
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• 제21권6호
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• pp.392-403
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• 2021

본 연구의 목적은 정서조절전략이 외상후 성장에 미치는 영향을 확인하고, 정서표현억제전략과 외상후 성장과의 관계에서 외상 경험에 대한 자기개방과 사회적 지지의 순차적 다중매개 효과를 확인하는 것이다. 이를 위해, 만 18세 이상의 성인을 대상으로 설문을 하여, 663명(남자 222명, 여자 441명)의 자료가 최종 통계분석에 사용되었다. SPSS Process Macro로 분석한 결과, 정서표현억제전략을 덜 사용할수록 외상후 성장이 더 높은 것으로 나타났다. 또한 정서표현억제전략과 외상후 성장과의 관계에서 자기개방의 단순매개효과, 정서표현억제전략과 외상후 성장과의 관계에서 자기개방과 사회적 지지의 순차적 다중매개효과를 확인하였다. 이는 정서조절전략으로서 정서표현억제를 적게 사용하는 사람들일수록 외상경험에 대해 자기개방을 많이 함으로써 주위 사람들의 위로와 지지를 받게 되어, 외상후 성장에 이를 수 있다는 것을 의미한다. 또한 이는 외상후 성장에 있어 정서적 자기개방과 사회적 지지가 중요함을 확인한 것이다. 평소에 정서표현을 억제하는 비자발적 내담자를 위한 외상후 성장을 촉진할 수 있는 상담현장에서의 개입방법을 제시하였고, 본 연구의 의의 및 한계점, 후속연구의 방향에 대해 제언하였다.

• 약학회지
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• 제41권1호
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• pp.94-98
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• 1997

Ceramide. a product of sphingomyelin hydrolysis, has been proposed as a lipid second messenger mediating antiproliferative activation. In this study, we examined the role of the cell cycle-related proteins in the ceramide-mediated growth suppression. Treatment of U-937 cells with C$_2$-ceramide(N-acetylsphingosine) resulted in growth suppression in a time- and concentration dependent manner. Ceramide induced concentration dependent dephosphorylation of retinoblastoma gene product (Rb). Rb remains hypophosphorylated in synchronized cells even after serum stimulation in the presence of ceramide. Ceramide decreased the expression of cyclin D$_1$ and cyclin E levels. These results suggest that antiproliferative effect of ceramide is associated with hypophosphorylation of Rb and decreased expression of cyclin D1 and cyclin E.

• Archives of Plastic Surgery
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• 제33권1호
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• pp.5-12
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• 2006

Protein tyrosine kinase(PTK), protein kinase C(PKC), oxidase, as a mediator, take a significant role in signal transduction pathway of angiogenesis. The authors utilized the inhibitors, targeting the formation of three co-enzyme in signal transduction pathway in order to quantify the suppression of abnormal vascular endothelial cell proliferation induced by DMH, to compare the level suppression in each up-regulated growth factors, CTGF, CYR61, $ITG{\beta}1$, FHL2, and to identify the relationship between abnormal cell proliferation and signal transduction pathway. Five groups were established; Control group, Group of DMH, Group of DMH-mixed Herbimycin, inhibitor of protein tyrosine kinase, Group of DMH-mixed Calphostin C, inhibitor of protein kinase C, Group Of Dmh-Mixed 10U Catalase, Inhibitor Of oxidase. The rise of vascular endothelial cell was compared by MTT assay, and four growth factors were analysed with RT-PCR method, at pre-administration, 4, 8, 12, and 24 hours after administration. In comparison of abnormal proliferation of vascular endothelial cell induced by DMH, suppression was noticed in Herbimycin and Calphostin C group, and Calphostin C group revealed higher suppression effect. Nevertheless, Catalase group did not have any suppression. In manifestation of four growth factors, Herbimycin and Calphostin C group presented similar manifestation with control group, except in $ITG{\beta}$. Catalse group had similar manifestation with DMH group in all four growth factors. Abnormal proliferation of vascular endothelial cell induced by DMH have a direct relationship with PTK and PKC, more specifically to PKC. Oxidase was confirmed not to have any relevance.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1994년도 Proceedings of International Symposium on BIOLOGICAL CONTROL OF PLANT DISEASES Korean Society of Plant Pathology
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• pp.27-49
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• 1994

Root colonization of biocontrol agents via seed treatment was investigated and a compatible combination, Gliocladium virens G872B and Pseudomonas putida Pf3, in colonizing cucumber rhizosphere was confirmed through the study. Much higher number of fungal and bacterial propagules were detected when two isolates were inoculated together. The presence of Pf3 in root system was greatly helpful to G872B to colonize at root tip. The mechanism of this phenomenon is partially elucidated through the results of in vitro experiments and the observations of scanning electron and fluorescence microscope. Addition of Pf3 cells resulted earlier germination of G872B conidia and increased mycelial growth. And the more number of germinated conidia on seed coat, the more vigorous hypal streching and sporulation on the root surface were observed in coinoculated treatment. The propagules of G872B on the cucumber root when they were challenged against the pathogenic Fusarium oxysporum, were even higher than that of G872B treated alone, and the magnitude of such a difference was getting grater toward the root ip and the population of F. oxysporum on the root was reduced by seed inoculation of G872B. The rhizosphere competence was obviously reflected to disease suppression and plant growth promotion that induced by the given isolate. Green house experiments revealed that the combined treatment provided long-term disease suppression with greater rate and the larger amount of fruit yield than single treatments. Through this study the low temperature growing Pseudomonas fluorescens M45 and MC07 were evaluated to apply them to the winter crops in field or plastic film house. In vitro tests reveal that M45 and MC07 inhibited the mycelial growth of Pythium ultimum, Rhizoctona solani and Phytophthora capsici and enhanced growth of cucumber cotyledon in MS agar. This effect was more pronounced when the bacteria were incubated at 14$^{\circ}C$ than at 27$^{\circ}C$. And disease suppression and plant growth promotion in green house were also superior at low temperature condition. Seed treatment of M45 or soil treatment of MC07 brought successful control of damping-off and enhanced seedling growth of cucumber. The combined treatment of two isolates was more effective than any single treatment.

• 원예과학기술지
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• 제17권5호
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• pp.572-577
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• 1999

여러 가지 생장조절제가 들잔디 직립생장에 미치는 영향을 규명함으로써 예초관리에 적절한 생장조절제 종류 및 적용수준을 파악하고자 본 연구를 수행하였다. 실험은 페어웨이 수준으로 유지되고 있는 들잔디 연구포장에서 1995년 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시한 3종류 생장조절제 모두 들잔디 생장을 억제하였지만, 억제기간은 종류에 따라 다르게 나타났다. 처리 1주 후 대부분의 생장조절제 처리구에서 약 10%에서 20% 정도의 억제효과가 있었다. Amidochlor 처리구는 $0.30mL{\cdot}m^{-2}$에서 $0.60mL{\cdot}m^{-2}$ 사이까지 3, 4주 정도 효과가 지속되었고, $0.08mL{\cdot}m^{-2}$ 이상의 trinexapacethyl 처리구에서는 8주까지 약 35% 정도의 억제효과가 관찰되었다. 다른 생장조절제에 비해 생장억제가 3, 4일 정도 빨리 나타난 mefluidide 처리구도 8주 정도 억제효과가 있었지만, 엽색 퇴화도 동시에 관찰되었다. 직립생장억제 관점에서 관리 정도가 낮게 유지되는 한국잔디에서 장기간 효과는 trinexapac-ethyl 처리가 amidochlor 및 mefluidide 보다 더 효과적인 것으로 사료되었다. 계절에 따라 생장조절제의 효과차이가 다르게 나타났는데, 들잔디 생육왕성기인 7월 처리에 비해 6월 처리시 직립경 생장억제 정도가 높게 나타났다. 본 연구를 통해 잔디 관리시 생장조절제 종류에 따라 30%에서 60% 정도 예초 회수를 감소시킬 수 있는 것으로 나타났으나, 예초 관리와 관련하여 기대하는 생장억제 기간에 따라 생장조절제 종류 및 적용 수준을 선택하는 것이 필요하다.

• 한국작물학회지
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• 제56권3호
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• pp.192-198
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• 2011

생육 중인 호밀 리빙멀치 사이 부분경운 기계화 콩 파종기술체계를 가지고 2007년은 호밀의 파종시기를, 2009년은 시험장소를 달리하면서 잡초억제 및 콩의 생육 및 수량에 미치는 영향을 조사하였는데, 중요한 사항을 요약하면 다음과 같다. 1. 4월초~5월초에 파종한 호밀 리빙멀치에 의한 콩 파종 후 40~50일까지의 잡초억제 효과는 70~90%로 현저히 높았는데, 호밀 파종기에 따른 잡초억제의 차이는 없었다. 또 호밀 리빙멀치의 잡초억제 효과는 콩 수확기 까지 지속되었다. 2. 잡초 초종별로는 잡초발생량의 60~80%를 차지하는 피에 대한 리빙멀치의 잡초억제 효과가 뛰어났으며 일찍 발아한 명아주보다 콩 파종 후 발아한 피, 바랭이 및 쇠비름에 대한 억제효과가 높았다. 3. 호밀을 4월 초에 파종한 것이 4월 말~5월 초에 파종한 것보다 콩 파종 시 리빙멀치량이 많았으며, 그에 따라 콩 초기생육이 많이 억제되었고, 수확기 종실수량의 감소에도 영향을 주는 것으로 나타났다. 4. 호밀을 4월 말~5월 초에 파종했을 때 콩의 초기생육은 억제되었지만 호밀이 고사한 후에 콩의 생육이 많이 회복되었고, 종실수량은 관행재배와 차이를 나타내지 않았다. 2008년 지역별 시험에서는 콩 종실수량은 호밀 리빙멀치 유무보다 지역의 포장조건에 따른 영향이 컸다. 5. 봄 잡초발생 경향, 리빙멀치량에 따른 잡초억제, 콩 초기생육 및 종실수량에 미치는 영향을 고려할 때 호밀 리빙멀치 사이 부분경운 기계화 콩 파종체계에서 호밀의 파종시기를 4월말 이전으로 당기는 것은 바람직하지 않았다.

• The Plant Pathology Journal
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• 제22권3호
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• pp.278-282
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• 2006

Bacillus vallismortis EXTN-1, a biocontrol agent in cucumber, tomato and potato was tested in rice pathosystem against rice fungal pathogens viz. Magnaporthe grisea, Rhizoctonia solani and Cochliobolus miyabeanus. Apart from increasing the yield in the bacterized plants (11.6-12.6% over control), the study showed that EXTN1 is effective in bringing about disease suppression against all the tested fungal pathogens. EXTN-l treatment resulted in 52.11% reduction in rice blast, 83.02% reduction in sheath blight and 11.54% decrease in brown spot symptoms. As the strain is proven as an inducer for systemic resistance based on PR gene expression in Arabidopsis and tobacco models, it is supposed that a similar mechanism works in rice, bringing about disease suppression. The strain could be used as a potent biocontrol and growth-promoting agent in rice cropping system.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.422-425
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• 1998

Chitin synthase null-mutants propagate in yeast form in RPMI medium with suppression of hyphal growth. This hyphal suppression is also observed in the wild type culture grown in RPMI medium supplemented with deer antler extract. To identify the possible target of deer antler extract, the enzymatic activities of chitin synthases were examined. The enzymatic activities of three chitin synthases, CAChsl, CAChs2, and CAChs3, were found to be differentially inhibited by deer antler extract. Of them, CAChsl, was the most sensitive to the extract. These results indicate that deer antler extract causes hyphal suppression, which resembles the effects of chitin synthase deletion, probably through direct inhibition of chitin synthases.