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A Study for the Mechanism of Abnormal Proliferation in Vascular Endothelial Cells using Inhibitors to the Signal Transduction Pathway  

Bae, Yong Chan (Department of Plastic and Reconstructive Surgery, Pusan National University)
Park, Suk Young (Department of Plastic and Reconstructive Surgery, Pusan National University)
Nam, Su Bong (Department of Plastic and Reconstructive Surgery, Pusan National University)
Herh, Jae Young (Department of Plastic and Reconstructive Surgery, Pusan National University)
Kang, Young Seok (Department of Plastic and Reconstructive Surgery, Pusan National University)
Publication Information
Archives of Plastic Surgery / v.33, no.1, 2006 , pp. 5-12 More about this Journal
Abstract
Protein tyrosine kinase(PTK), protein kinase C(PKC), oxidase, as a mediator, take a significant role in signal transduction pathway of angiogenesis. The authors utilized the inhibitors, targeting the formation of three co-enzyme in signal transduction pathway in order to quantify the suppression of abnormal vascular endothelial cell proliferation induced by DMH, to compare the level suppression in each up-regulated growth factors, CTGF, CYR61, $ITG{\beta}1$, FHL2, and to identify the relationship between abnormal cell proliferation and signal transduction pathway. Five groups were established; Control group, Group of DMH, Group of DMH-mixed Herbimycin, inhibitor of protein tyrosine kinase, Group of DMH-mixed Calphostin C, inhibitor of protein kinase C, Group Of Dmh-Mixed 10U Catalase, Inhibitor Of oxidase. The rise of vascular endothelial cell was compared by MTT assay, and four growth factors were analysed with RT-PCR method, at pre-administration, 4, 8, 12, and 24 hours after administration. In comparison of abnormal proliferation of vascular endothelial cell induced by DMH, suppression was noticed in Herbimycin and Calphostin C group, and Calphostin C group revealed higher suppression effect. Nevertheless, Catalase group did not have any suppression. In manifestation of four growth factors, Herbimycin and Calphostin C group presented similar manifestation with control group, except in $ITG{\beta}$. Catalse group had similar manifestation with DMH group in all four growth factors. Abnormal proliferation of vascular endothelial cell induced by DMH have a direct relationship with PTK and PKC, more specifically to PKC. Oxidase was confirmed not to have any relevance.
Keywords
Proliferation; Angiogenic growth factor; Vascular endothelial cells;
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Times Cited By KSCI : 3  (Citation Analysis)
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