• Title/Summary/Keyword: growth regulator

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Establishment of Analytical Method for Residues of Ethychlozate, a Plant Growth Regulator, in Brown Rice, Mandarin, Pepper, Potato, and Soybean Using HPLC/FLD

  • Kim, Jae-Young;Lee, Jin Hwan;Lee, Sang-Mok;Chae, Young-Sik;Rhee, Gyu-Seek;Chang, Moon-Ik
    • Korean Journal of Environmental Agriculture
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    • v.34 no.2
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    • pp.111-119
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    • 2015
  • BACKGROUND: Ethychlozate (ECZ) is a plant growth regulator of synthetic auxin for agricultural commodities (ACs). Accurate and sensitive method to determine ECZ in diverse ACs on global official purpose is required to legal residue regulation. As the current official method is confined to the limited type of crops with poor validation, this study was conducted to improve and extend the ECZ method using high-performance liquid chromatography (HPLC) in all the registered crops with method verification. METHODS AND RESULTS: ECZ and its acidic metabolite (ECZA) were both extracted from acidified samples with acetone and briefly purified by dichloromethane partition. ECZ was hydrolyzed to form ECZA and the combined ECZA was finally purified by ion-associated partition including hexane-washing. The instrumental quantitation was performed using HPLC/ FLD under ion-suppression of ECZA with no interference by sample co-extractives. The average recoveries of intra- and inter-day experiment ranged from 82.0 to 105.2% and 81.7 to 102.8%, respectively. The repeatability and reproducibility for intra- and inter-day measurements expressed as a relative standard deviation was less than 8.7% and 7.4%, respectively. CONCLUSION: Established analytical method for ECZ residue in ACs was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability and reproducibility.

Studes on the Hardwood Cutting of Mulberry(Morus alba L.) I. Effect of Cutting Methods and Seasons n Rootability (뽕나무 고조섭목에 관한 연구 I. 섭목방법 및 시기가 활착률에 미치는 영향)

  • 이동한;김동일
    • Journal of Sericultural and Entomological Science
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    • v.28 no.2
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    • pp.9-14
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    • 1986
  • The rootability of hardwood cutting of mulberry (Morus alba L.) depending upon the kinds of cutting beds, soil of cutting beds, effect of growth regulator (Rootone-F), pretreatments of cutting scion, parts of branches were investigated, The results were as follows ; 1. Rootability was the highest in cutting after callus formed on sand bed, whereas null in the treatment of rice seedling hot bed. 2. Optimum cutting season was around March 26, showed 100% rootability in all treatments except wooden box filled with sand. 3. The order of rootability related with cutting part of branch was upper$^{\circ}C$), and cutting after formation of callus increased rootability, whereas cuttings left in open air for 5 days showed null rootability.

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Na/K-ATPase beta1-subunit associates with neuronal growth regulator 1 (NEGR1) to participate in intercellular interactions

  • Cheon, Yeongmi;Yoo, Ara;Seo, Hyunseok;Yun, Seo-Young;Lee, Hyeonhee;Lim, Heeji;Kim, Youngho;Che, Lihua;Lee, Soojin
    • BMB Reports
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    • v.54 no.3
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    • pp.164-169
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    • 2021
  • Neuronal growth regulator 1 (NEGR1) is a GPI-anchored membrane protein that is involved in neural cell adhesion and communication. Multiple genome wide association studies have found that NEGR1 is a generic risk factor for multiple human diseases, including obesity, autism, and depression. Recently, we reported that Negr1-/- mice showed a highly increased fat mass and affective behavior. In the present study, we identified Na/K-ATPase, beta1-subunit (ATP1B1) as an NEGR1 binding partner by yeast two-hybrid screening. NEGR1 and ATP1B1 were found to form a relatively stable complex in cells, at least partially co-localizing in membrane lipid rafts. We found that NEGR1 binds with ATP1B1 at its C-terminus, away from the binding site for the alpha subunit, and may contribute to intercellular interactions. Collectively, we report ATP1B1 as a novel NEGR1-interacting protein, which may help deciphering molecular networks underlying NEGR1-associated human diseases.

Effect of Several Fungicides and Growth Regulators on Rice Seedling Growth and Damping-off in Seedling Boxes for Machine Transplanting (벼상자육묘(箱子育苗)에서 살균제(殺菌齊)와 생장조절제(生長調節劑) 처리(處理)가 묘생육(苗生育) 및 생리장해(生理障害)에 미치는 영향(影響))

  • Jeh, Sang Yull;Hwang, Chung Dong
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.1-11
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    • 1987
  • This study was conducted to evaluate the effect of several fungicides and growth regulators on rice seedling growth and damping-off in seedling boxes for machine transplanting. Fungicide treated plots were better seedling growth, shoot regrowth, rooting ability, change of moisture content than those of nontreated plot. Metalaxyl application of Samgangbyeo and SF8002 application of Nagdongdyeo apparently increased plant height, length of the third leaf and fourth leaf. And metaiaxyl application highly increased dry weight. Fungicide treated plots were highly effective in reducing the incidence of damping-off. Benzyladenine application of Samgangbye and $GA_3$ application of Nagdongbyeo apprently increased plant height. But ABA application highly decreased plant height. ABA application and aCE application resulted in highly increased rooting ability. Fungicide and $GA_3$ treated plots, Metalaxyl and growth regulator treated plots resulted in highly increased plant height. I soprothiolane and growth regulator treated plots resulted in decreased plant height. Dachigaren and lAA treated plot apprently increased dry weight and shoot dry weight/plant height. Fungicide and growth regulator treated plots were highly effective in reducing the incidence of damping-off.

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High Frequency Somatic Embryogenic Callus Induction and Plant Regeneration from Various Indica Rice Genotypes

  • Hoque Md. Enamul;Mansfield John W.
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.257-262
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    • 2006
  • The paper evaluated the behavior of in vitro culture responses from a diverse set of Indica rice (Oryza sativa L.) genotypes. Significant differences were found in embryogenic callus induction frequency, callus growth and plant regeneration frequency when mature embryos of 11 cultivars, breeding lines and land races were compared. Genotype as well as plant growth regulator influenced the plant regeneration frequency. Callus induction frequency was not correlated with callus growth as well as plant regeneration frequency. The regenerated plants could grow to normal, fertile plants after they were successfully established in soil.

ESTABLISHMENT OF IN VITRO BIOASSAY FOR TRANSFORMING GROWTH FACTOR (TGF-$\varepsilon$)

  • Kim, Mi-Sung;Ahn, Seong-Min;Aree Moon
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.10a
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    • pp.201-201
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    • 2001
  • Transforming growth factor- $\beta$ (TGF- $\beta$), a hormonally active polypeptide found in normal and transformed tissue, is a potent regulator of cell growth and differentiation. In this study, we wished to establish an in vitro bioassay system to seek the most sensitive method that can measure TGF- $\beta$ activity.(omitted)

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The role of autophagy in the placenta as a regulator of cell death

  • Gong, Jin-Sung;Kim, Gi Jin
    • Clinical and Experimental Reproductive Medicine
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    • v.41 no.3
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    • pp.97-107
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    • 2014
  • The placenta is a temporary fetomaternal organ capable of supporting fetal growth and development during pregnancy. In particular, abnormal development and dysfunction of the placenta due to cha nges in the proliferation, differentiation, cell death, and invasion of trophoblasts induce several gynecological diseases as well as abnormal fetal development. Autophagy is a catalytic process that maintains cellular structures by recycling building blocks derived from damaged microorganelles or proteins resulting from digestion in lysosomes. Additionally, autophagy is necessary to maintain homeostasis during cellular growth, development, and differentiation, and to protect cells from nutritional deficiencies or factors related to metabolism inhibition. Induced autophagy by various environmental factors has a dual role: it facilitates cellular survival in normal conditions, but the cascade of cellular death is accelerated by over-activated autophagy. Therefore, cellular death by autophagy has been known as programmed cell death type II. Autophagy causes or inhibits cellular death via the other mechanism, apoptosis, which is programmed cell death type I. Recently, it has been reported that autophagy increases in placenta-related obstetrical diseases such as preeclampsia and intrauterine growth retardation, although the mechanisms are still unclear. In particular, abnormal autophagic mechanisms prevent trophoblast invasion and inhibit trophoblast functions. Therefore, the objectives of this review are to examine the characteristics and functions of autophagy and to investigate the role of autophagy in the placenta and the trophoblast as a regulator of cell death.