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http://dx.doi.org/10.5483/BMBRep.2021.54.3.116

Na/K-ATPase beta1-subunit associates with neuronal growth regulator 1 (NEGR1) to participate in intercellular interactions  

Cheon, Yeongmi (Department of Microbiology and Molecular Biology, Chungnam National University)
Yoo, Ara (Department of Microbiology and Molecular Biology, Chungnam National University)
Seo, Hyunseok (Department of Microbiology and Molecular Biology, Chungnam National University)
Yun, Seo-Young (Department of Microbiology and Molecular Biology, Chungnam National University)
Lee, Hyeonhee (Department of Microbiology and Molecular Biology, Chungnam National University)
Lim, Heeji (Department of Microbiology and Molecular Biology, Chungnam National University)
Kim, Youngho (Department of Microbiology and Molecular Biology, Chungnam National University)
Che, Lihua (Department of Microbiology and Molecular Biology, Chungnam National University)
Lee, Soojin (Department of Microbiology and Molecular Biology, Chungnam National University)
Publication Information
BMB Reports / v.54, no.3, 2021 , pp. 164-169 More about this Journal
Abstract
Neuronal growth regulator 1 (NEGR1) is a GPI-anchored membrane protein that is involved in neural cell adhesion and communication. Multiple genome wide association studies have found that NEGR1 is a generic risk factor for multiple human diseases, including obesity, autism, and depression. Recently, we reported that Negr1-/- mice showed a highly increased fat mass and affective behavior. In the present study, we identified Na/K-ATPase, beta1-subunit (ATP1B1) as an NEGR1 binding partner by yeast two-hybrid screening. NEGR1 and ATP1B1 were found to form a relatively stable complex in cells, at least partially co-localizing in membrane lipid rafts. We found that NEGR1 binds with ATP1B1 at its C-terminus, away from the binding site for the alpha subunit, and may contribute to intercellular interactions. Collectively, we report ATP1B1 as a novel NEGR1-interacting protein, which may help deciphering molecular networks underlying NEGR1-associated human diseases.
Keywords
Cancer; Cell adhesion molecule; Lipid rafts; Na/K-ATPase beta1-subunit; Neuronal growth regulator 1; Yeast two-hybrid;
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