• Molecules and Cells
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• v.21 no.1
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• pp.29-33
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• 2006

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack $p16^{INK4a}$ regulatory function, compared to primary BME cells that were growth arrested by enforced expression of $p16^{INK4a}$. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

• Journal of Convergence for Information Technology
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• v.8 no.6
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• pp.15-20
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• 2018

This study aimed to find an effective intervention measure through establishing the correlation between self-regulation (control over life style) and height growth through neuro-feedback training. 40 elementary students in grades two to four with height growth programs (20 experimental group students, 20 control group students) were examined for the changes before and after undergoing neuro-feedback training. The experiment lasted for three months with one 30-minute training session two times a week. After analyzing the differences in self-regulation among the control group with no neuro-feedback training and the experimental group with neuro-feedback training, the differences in height growth were analyzed. First of all, there were positive changes in self-regulation of the experimental group compared with the control group. Secondly, the experimental group showed larger changes in height growth. In conclusion, neuro-feedback training had positive effects upon the self-regulation that adjusts the acquired factors of height growth, which led to positive effects.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.29 no.3
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• pp.1-14
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• 2021

This study examined the mediating role of cognitive emotion regulation in the relationship between calling and posttraumatic growth (PTG) and the moderating role of transformational leadership among Air Force pilots. A total of 215 ROK Air Force pilots participated in this study twice with an interval of 4 weeks. The results of this study were as follows. First, calling, transformational leadership, adaptive emotion regulation, and PTG showed statistically significant correlations. Second, a mediating model showed that the relationship between calling and PTG was mediated by adaptive emotion regulation. Third, the moderation effect of transformational leadership in the relationship calling on adaptive emotion regulation was found. Finally, transformational leadership also moderated the mediating effect of calling on PTG through adaptive emotion regulation was identified. Implications, limitations, and future research suggestions were discussed.

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.249-255
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• 2006

Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1231-1240
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• 2021

Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

• Journal of Digital Convergence
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• v.17 no.10
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• pp.479-487
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• 2019

The study sought to reveal the impact of focusing attitudes and cognitive emotion regulation on post-traumatic growth for adolescent athletes with trauma experiences. A preliminary survey was conducted on 215 middle and high school athletes in the S city and K provinces. The impact of focusing attitudes and cognitive emotion regulation on post-traumatic growth was verified using validated questionnaires. The results for the purpose and method of study are as follows. Focusing attitudes and cognitive emotion regulation of adolescent athletes affect post-traumatic growth. Positive reappraisal was the most influential factor affecting post-traumatic growth, followed by the factors influencing the aware, acceptance, accept, reflect and positive refocusing. In order to experience post-traumatic growth through the results of this study, it is important to understand the overall understanding and tactics of focusing attitudes and cognitive emotion regulation. Therefore, effective psychological training methods will be needed to positively control the level of focusing attitudes and cognitive emotion regulation among adolescent athletes.

• Journal of Photoscience
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• v.9 no.2
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• pp.94-97
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• 2002

Light is one of the most important environmental factors that influence plant growth and development. It does not function independently but exerts its role through coordinated interactions with intrinsic developmental programs, such as hormonal regulation. One typical example is hypocotyl growth in which light signals are modulated through growth hormones. However, the underlying molecular mechanisms are largely unknown. We demonstrated that brassinosteroids play an important role in the light signal transduction in etiolated hypocotyl growth. A light-responsive Ras-like G-protein, Pra2 from pea, physically and functionally interacts with a cytochrome P450 that specifically catalyzes C-2 hydroxylation in brassinosteroid biosynthesis. The cytochrome P450 expression, along with Pra2, is induced in the dark and predominantly localized in the rapidly elongating zone of etiolated pea epicotyls. Transgenic plants with a reduced level of Pra2 exhibit a dark-specific dwarfism, which is completely rescued by brassinosteroid application. On the contrary, overexpression of the cytochrome P450 results in enhanced hypocotyl growth even in the light, which phenocopies the etiolated hypocotyl growth. It is therefore envisioned that Pra2 is a molecular switch that mediates the crosstalk between light and brassinosteroids in the etiolation process.

• Journal of Energy Engineering
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• v.29 no.1
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• pp.1-12
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• 2020

The rapid climate change is strengthening carbon emissions regulations internationally. Korea is strongly pressed to accept the obligation to reduce greenhouse gases as one of the United Nations Framework Convention on Climate Change. This article analyzed the Granger causalities among environmental regulation, economic growth, electricity consumption, and CO₂ emission in Korea, using unit root test, cointegration test, and vector error correction model. As the results, environmental regulation has shown the bidirectional causalities with electricity consumption and CO₂ emission, while being unilaterally affected by economic growth in the long-run and strong relationship. Economic growth has affected electricity consumption, CO2 emission, and environmental regulation in the long-run, in the complex structure of the unilateral and short-run causality with electricity consumption and the bidirectional causality with CO2 emission. The policy implications will be as follows: ① environmental regulation should induce sustainable growth through encouraging technological innovation relating to CO2 reduction and productivity enhancement. ② Responding to the international CO2 reduction regulation, the synthetic policy initiatives will be considered to make synergy effects among policies relating to economic growth, electricity consumption.

• BMB Reports
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• v.49 no.11
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• pp.598-606
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• 2016

ARF is an alternative reading frame product of the INK4a/ARF locus, inactivated in numerous human cancers. ARF is a key regulator of cellular senescence, an irreversible cell growth arrest that suppresses tumor cell growth. It functions by sequestering MDM2 (a p53 E3 ligase) in the nucleolus, thus activating p53. Besides MDM2, ARF has numerous other interacting partners that induce either cellular senescence or apoptosis in a p53-independent manner. This further complicates the dynamics of the ARF network. Expression of ARF is frequently disrupted in human cancers, mainly due to epigenetic and transcriptional regulation. Vigorous studies on various transcription factors that either positively or negatively regulate ARF transcription have been carried out. However, recent focus on posttranslational modifications, particularly ubiquitination, indicates wider dynamic controls of ARF than previously known. In this review, we discuss the role and dynamic regulation of ARF in senescence and cancer.

• International Journal of Computer Science & Network Security
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• v.22 no.4
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• pp.67-72
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• 2022

The main purpose of the study is to analyze the main aspects of state regulation of the banking business in the context of social and digital transformation. One of the key elements of the functioning of the economy of any country are banks that ensure the redistribution of financial resources and stimulate economic growth. However, the banking sector, like other activities, is dynamic and depends on the pace of development and forms of technological progress that affect the forms and types of information and digital technologies, as well as the globalization and remoteness of banking services. Accordingly, the need for effective implementation of the latest technologies becomes relevant, which will not only help increase consumer satisfaction with the banking product, but also ensure the development of the country's financial sector. As a result of the study, trends in the development of state regulation of the banking sector in the digital economy were identified.