Kim, So-Young;Noh, Yong-Ho;Kang, Sung-Gak;Kim, Young-Bum;Jang, Woo-Jin;Kim, Dong-Joon;Yun, Hyun-Shik
KSBB Journal
/
v.22
no.3
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pp.162-167
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2007
Ammonia gas is one of the major pollutants which cause environmental pollution and damage to the human and the livestock. The objective of this study was to investigate the important parameters for the development of efficient removal of ammonia gas by Bacillius subtilis IB101 and to optimize the medium composition for the mass production of B. subtilis IB101. The ammonia gas removal efficiency was evaluated at different growth phases and by changing culture conditions (temperature, pH). The effect of $(NH_4)_2SO_4$ concentration in preculture medium was examined. Medium optimization for the mass production of B. subtilis IB101 was performed by using Plackett-Burman design and one factor at a time method. The removal of ammonia gas was more efficient at exponential phase by 20% than at stationary phase. The ammonia gas removal was the highest at pH 4 and 30 $^{\circ}C$. There was not any significant influence of concentration of $(NH_4)_2SO_4$ on the removal of ammonia gas. The components of optimized medium for the production of viable Bacillus subtilis IB101 was yeast extract 10 g/l, soluble starch 2.5 g/l, $MgSO_4$ 6 g/l, $CaCl_2$ 1.55 g/l, $(NH_4)_2SO_4$ 5 g/l, $KH_2PO_4$ 0.75 g/l, soy bean meal 8 g/l.
Journal of the Korean Society for Marine Environment & Energy
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v.19
no.2
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pp.87-98
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2016
During the period between July 3 and 27 of 2009, water samples were collected from the Russian coast at a depth of 30m from 26 stations (including Ulleung and Japan basins) onboard the Russian survey vessel R/V Lavrentyev following 4 lines (D, R, E, and A). The samples were analyzed for nutrients and chlorophyll a contents. All parameters exhibited higher values in warm waters than in cold waters ($NH_4:1.8-fold$, $PO_4:1.8-fold$, $SiO_2:1.2-fold$, and chlorophyll-${\alpha}$:1.9-fold), except nitrates, which was 1.4-fold higher in cold waters than in warm waters. The horizontal distribution of ammonia, phosphate, and chlorophyll-${\alpha}$ was very similar to each other and showed the highest values in the waters near Russia, where a upwelling influence of cold current and bottom water prevails, while relatively low distribution was observed at the Ulleung Basin. On the other hand, nitrates showed the highest concentration at the Ulleung Basin, which is under the direct influence of the Tsushima warm water, and showed a gradual decrease northward. The N/P ratio showed the highest value in the Tsushima middle water, rather than in the North Korean Cold Water, the Tsushima Warm Water was the primary source of nitrate flow into the East Sea. However, the average concentration of phosphate in the warm waters was < $0.2{\mu}M$, thereby limiting phytoplankton growth, while a high concentration of phosphate in cold waters showed a direct correlation with chlorophyll-${\alpha}$. The results of principal component analysis for the identification of primary factors that influence the marine environment showed that principal component I was water temperature and principal component II was influenced chlorophyll-${\alpha}$ and nutrients. Therefore, Study area has greatest influenced by water temperature, and clearly distinct cold and warm water regions were observed in the East Sea.
Lack of agricultural information for food supply and demand in Democratic People's republic Korea(DPRK) make people sometimes confused for right and timely decision for policy support. We carried out a study to estimate paddy rice yield in DPRK using MODIS NDVI reflecting rice growth and climate data. Mean of MODIS $NDVI_{max}$ in paddy rice over the country acquired and processed from 2002 to 2014 and accumulated rainfall collected from 27 weather stations in September from 2002 to 2014 were used to estimated paddy rice yield in DPRK. Coefficient of determination of the multiple regression model was 0.44 and Root Mean Square Error(RMSE) was 0.27 ton/ha. Two-way analysis of variance resulted in 3.0983 of F ratio and 0.1008 of p value. Estimated milled rice yield showed the lowest value as 2.71 ton/ha in 2007, which was consistent with RDA rice yield statistics and the highest value as 3.54 ton/ha in 2006, which was not consistent with the statistics. Scatter plot of estimated rice yield and the rice yield statistics implied that estimated rice yield was higher when the rice yield statistics was less than 3.3 ton/ha and lower when the rice yield statistics was greater than 3.3 ton/ha. Limitation of rice yield model was due to lower quality of climate and statistics data, possible cloud contamination of time-series NDVI data, and crop mask for rice paddy, and coarse spatial resolution of MODIS satellite data. Selection of representative areas for paddy rice consisting of homogeneous pixels and utilization of satellite-based weather information can improve the input parameters for rice yield model in DPRK in the future.
Kim, Byong-Wan;Oh, Jin-Seok;Han, Ohan-Taek;Park, Sang-Oh;Park, Byung-Sung
Korean Journal of Organic Agriculture
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v.17
no.1
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pp.111-125
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2009
Pitamin is a component of pine bark extract that exhibits antimicrobial activity and a variety of physiological effects. This study was earned out to investigate the effects of dietary pitamin as an organic livestock feed additive in broiler chickens. A 35 day trial was conducted to determine the influence of dietary premix containing 5% pitamin; investigated parameters included blood lipids, growth performance, quality characteristics of carcasses, and changes of caecal microbials in broiler chickens. Chickens were randomly divided into groups that were untreated (control), treated conventionally with antibiotics in the absence of premix, received 0.1 % or 0.2% premix containing 5% pitamin. Plasma lipids were lower in groups fed diets with pitamin premix (p<0.05). The body weight gain from broiler chickens fed with the diet containing 0.1% pitamin premix and antibiotics was similar, and was significantly higher than that of the other groups (p<0.05). The weight of breast muscle and thigh meat of carcasses was similar, and was higher than that of the control group (p<0.05). Abdominal fat and thymus index from chickens receiving either pitamin-supplemented premix was significantly lower and increased, respectively, that of the antibiotic and control groups (both p<0.05). The chickens on the pitamin premix-supplemented diets evidenced significantly higher caecal levels of Bifidobacterium species as compared with the chickens on the control diet (p<0.05). These results suggest that feeding a diet supplemented with a 0.1% premix containing 5.0% pitamin for 35 days maintains the production of broiler chickens at a level comparable to that obtained from the use of antibiotics.
Two hundred fifty eight Hanwoo steers were used in a completely randomized design experiment to determine the effects of ad libitum or restricted feeding of concentrates on body weight(BW) gain, feed intake, blood metabolites and hematological parameters. Steers were assigned at 6 months of age to feeding groups of ad libitum(T1) or restricted(T2) by 18 months of age. Steers in both groups were fed ad libitum from 19 months of age. The restrictive feeding levels were 1.2-1.5% of BW for the growing period and 1.7-1.8% of BW for the early fattening period. Average daily gains were significantly higher in T1 than in T2 from 10 to 14 months of age, but were significantly higher in T2 than in T1 from 20 to 24 months of age(p<0.05). Total dry matter intake(DMI) was higher in T1 than in T2 at 10, 12 and 16 months of age(p<0.05). Total DMI of T2 was higher than that of T1 at 22 months of age(p<0.05). Feed conversions were significantly lower in T2 than in T1 from 20 to 30 months of age(p<0.05). Blood albumin concentrations were significantly higher in T2 than in T1 at 12, 14, 16 and 18 months of age. Blood triglyceride concentrations were significantly higher in T1 than in T2 at 14 and 16 months of age(p<0.05). Blood inorganic phosphorus concentrations were significantly higher in T2 compared with T1 at 8, 10, 16 and 22 months of age(p<0.05). Mean corpuscular volume and mean corpuscular hemoglobin were significantly lower in T2 than in T1 from 8 to 12 months of age(p<0.05), but those were significantly higher in T2 than T1 from 10 months to 12 months of age(p<0.05). Present results may indicate that the restricted feeding for the growing period does not show adverse effects on body weight gain with better feed conversion for the following late fattening period.
A model of pond system is developed for treatment and recycling of excreta from twenty-five adult dairy cattle. It is composed of wastewater treatment ponds and small fish ponds. Those are three facultative ponds in series; primary-secondary-tertiary pond and these are designed to rear carps without feeding. A pit is constructed at the bottom of primary pond for efficient sludge sedimentation and effective methane fermentation. It is contrived to block into it the penetration of oxygen dissolved in the upper layer of pond water. The excreta from the cattle housed in stalls are diluted by water used for clearing them. The washed excreta flow into the pit. The average yearly $BOD_5$ concentration of influent is 398.7mg/l. That of the effluent from primary, secondary and tertiary pond of the system is 49.18, 27.9, and 19.8.mg/l respectively. Approximate 88, 93, and 95 % of BOD5 are removed in each pond. The mean yearly SS concentration of influent is 360.5 mg/l That of the effluent from each pond is 53.4, 45.7, and32.7mg/l respectively. Approximate 86, 88, and 91% of SS are removed in each pond. The $BOD_5$ concentration of secondary and tertiary pond can satisfy 30mg/l secondary treatment standard. The SS concentration of effluent from tertiary pond, however, is slightly greater than the standard, which results from activities of carps growing in the pond. The average yearly total nitrogen concentration of influent is 206.8mg/l and that of the effluent from each pond is 48.6, 30.8, and 21.0mg/l respectively. Approximate 74, 88, and 90% of total nitrogen are removed in each pond. The mean yearly total phosphorous concentration of influent is 20.7mg/l and that of the effluent from each pond is 5.3, 3.2, and 2.1mg/l respectively. Approximate 97, 98, and 99% of total phosphorous are removed in each pond. The high removal of nitrogen and phosphorous results from active growth of algae in the upper layer of pond water. Important pond design parameters for southern part of Korea -- areal loading of BOD5, liquid depth, hydraulic detention time, free board, and pond arrangement -- are taken up.
Background : Tumor growth is the net result of intrinsic proliferation and escape from active cell death. bcl-2 is a member of a new category of oncogenes that is not involved in influencing cell proliferation but is involved in regulating cell death(apoptosis). Based on this information, it seems to be reasonable to expect that there may be clinical prognostic significance of bcl-2 expression in non-small cell lung cancer. But its prognostic significance is not established. Methods: To investigate the role of bcl-2 in lung cancer, we performed immunohistochemical stain of bcl-2 on 57 biopsy specimens from resected primary non-small cell lung cancer. Thereafter, flow cytometric cell cycle analysis was done. And we analyzed the correlation between bcl-2 expression, clinical parameters, S-, $G_1$-phase fraction and survival. Results: bcl-2 were detected in 43.8% of total 57 patients(according to histology, squamous cancer 47%, adenocarcinoma 32%, according to TNM stage, I 28.6%, II 52.3%, III 45.5%. both differences were insignificant). By using the flow cytometric analysis, mean S-phase fraction of bcl-2(+) and (-) group were 14.1($\pm7.8$)%, 24.7($\pm10.5$)% (p<0.005), mean $G_1$-phase fraction of bcl-2(+) and bcl-2(-) group were 75.5($\pm10.8$)%, 65.5($\pm11.4$)%(p<0.05). 2yr, 3yr and 5yr survival and median survival time of bcl-2(+) group were 65%, 54%, 41%, 53 months, and those of bcl-2(-) group were 71%, 52%, 46%, 37 months. (p>0.05, Kaplan-Meier, log rank) Conclusion: bcl-2 was detected in 43.8% of primary non-small cell lung cancer. The S-phase fraction of bcl-2(+) group was less than bcl-2(-) group, and G1-phase fraction of bcl-2(+) group was more than bcl-2(-) group. But, expression of bcl-2 could not be a prognostic factor.
To improve competitiveness & performance for salesmen of small & medium IT company, this study aims not only to inspect how value orientation, leadership & justice make effects for Organizational Citizenship Behavior & Business Corporate Performance & but also to explore the role of adaptive selling practices as parameter. To support the study, the data collected from 314 employees in sales roles at more than 200 IT companies was processed via. regression analysis method. The research model of study lies at identification of 'the Effects of Value Orientation, Leadership, & Justice of/Posed by the Salesmen of a IT Company on Organizational Citizenship Behavior & Corporate Performance' based on the phenomena of unfair sales strategies rampantly being taken for short-term profits & survivals despite of the value of upholding business ethics to realize long-term, sustainable growth of a business of company. The hypotheses of this study are formulated as follows. First, value orientation, leadership, & justice shall have effects on organizational citizenship behavior & Corporate performance. Second, adaptive selling practices shall function as the parameters between the independent & dependent variables. The analysis results on the research, undertaken with verification of parametric effects, confirm the following: 1. Value orientation imposes positive (+) effects on adaptive selling practices which impose positive (+) impacts on organizational citizenship behavior & Corporate performance. 2. Adaptive selling practices function as a full parameter between value orientation & organizational citizenship behavior whilst functioning as a partial parameter between value orientation & Corporate performance. 3. Leadership imposes positive (+) effects on adaptive selling practices which impose positive (+) effects on organizational citizenship behavior & Corporate performance. 4. Adaptive selling practices function as a partial parameter between leadership & organizational citizenship behavior whilst functioning as a full parameter between leadership & Corporate performance. Therefore, this study is concluded that establishing & executing sales strategies in consideration of value orientation & fairness is of extreme importance for IT companies to realize & maintain their sustainable corporate management, & last but not least, it is necessary for IT companies to proactively introduce & provide educational systems for their salesmen thus to help them to uphold & sustain ethics & values of the business.
This experiment was conducted to investigate the effect of feeding monensin on the growth performance and ruminal fermentation characteristics of Han-Woo cattle. Seventy two uncastrated Han-Woo male cattle(BW 267 kg) were randomly allotted to 0, 22, and 33 ppm monensin treatments, three replicates per treatment and eight heads per replicate. Animals were kept in an open barn for an 140-d feeding trial, Concentrates containing different levels of monensin and rice straw cut in 15cm length were fed ad libitum separately. The results obtained from this study were summarized as follows. 1. No significant difference was found in daily gain by monensin feeding. 2. Monensin did not affect the total feed (concentrate + roughage) intake: however, as the monensin level increased, the total feed intake tended to decrease, resulting in 5 % reduction in 33 ppm monensin treatment. 3. Although no significant difference was found among three treatments, 22 and 33 ppm monensin improved the feed efficiency(total feed/gain) by 5.2 % and 5.1 %, respectively, as compared to the 0 ppm monensin treatment. 4. Monensin did not affect the concentrations of ruminal total VFA and acetic acid consistently. Although not significant, monensin feeding of 22 and 33 ppm caused marked increase in ruminal propionic acid concentration, 13.8 % and 19.3 %, respectively. Ruminal butyric acid concentration decreased as monensin level increased. Monensin feeding, regardless of level, decreased the A/P ratio by 12.5 %. In conclusiuon, monensin feeding increased the propionic acid concentration, and decreased the butyric acid concentration and A/P ratio in the rumen. Animals fed monensin consumed less feed, causing the improvement in feed efficiency. Thus, monensin appeared to be a useful feed additive, directing the rumen fermentation in a more productive way. Feed efficiency improved similarly both in 22 and 33 ppm monensin treatments, indicating that 22 ppm might be good enough rather than the 33 ppm monensin level.
This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Aster scaber and Synurus deltoides leaves by using Pressure-volume curves. In order to measure pressure-volume (P-V) curves, Aster scaber and Synurus deltoides were cut off above ground part and the tip of the cutting were placed in water, which was covered with a plastic bag. Samples were kept overnight (about 12 hours) in darkness at room temperature (20~25$^{\circ}C$) to achieve maximal turgor (full saturation). The pressure in the chamber was gradually increased from 0.3MPa to 1.8MPa by nitrogen gas. After measured, leaf samples were dried at 80$^{\circ}C$ for 48 hours and dry weight of each samples were determined. The result of the original bulk osmotic potential at maximum turgor ${\Psi}^{sat}_o$ sat was lower -0.8 MPa in Aster scaber leaves than -0.7 MPa Synurus deltoides leaves. Also the osmotic potential at incipient plasmolysis ${\Psi}^{tlp}_o$ in Aster scaber leave was -0.9 MPa. In contrast, the value of maximum bulk modulus of elasticity $E_{max}$ of Aster scaber leaves were approximately two folds higher than that of Synurus deltoides leaves. The values of the relative water content at incipient plasmolysis $RWC^{tlp}$ are all above 90% showing that the function of osmoregulation is somewhat better, and Vo/DW, Vt/DW, Ns/DW of Synurus deltoides leaves were approximately 1~2 times higher than that of Aster scaber leaves. Thus, responses to water relations of Aster scaber and Synurus deltoides such as ${\Psi}^{sat}_o$, ${\Psi}^{tlp}_o$, $E_{max}$, ${\Psi}_{P,max}$, $RWC^{tl}$ were shown that the Aster scaber leaves was slightly higher drought-tolerance than Synurus deltoides leaves. However, in both of Aster scaber and Synurus deltoides, occurring incipient plasmolysis at the high water content, have a relatively lower drought-tolerance property indicating that growth of these plants are cultivated appropriate in high moisture soil sites.
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