Browse > Article

Ammonia Gas Removal by Bacillus subtilis IB101 and Dctimization of Culture Media  

Kim, So-Young (Department of Biological Engineering, Inha University)
Noh, Yong-Ho (Department of Biological Engineering, Inha University)
Kang, Sung-Gak (Department of Biological Engineering, Inha University)
Kim, Young-Bum (Institute of Biotechnology, MG Intobio Co.)
Jang, Woo-Jin (Institute of Biotechnology, MG Intobio Co.)
Kim, Dong-Joon (Institute of Biotechnology, MG Intobio Co.)
Yun, Hyun-Shik (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.22, no.3, 2007 , pp. 162-167 More about this Journal
Abstract
Ammonia gas is one of the major pollutants which cause environmental pollution and damage to the human and the livestock. The objective of this study was to investigate the important parameters for the development of efficient removal of ammonia gas by Bacillius subtilis IB101 and to optimize the medium composition for the mass production of B. subtilis IB101. The ammonia gas removal efficiency was evaluated at different growth phases and by changing culture conditions (temperature, pH). The effect of $(NH_4)_2SO_4$ concentration in preculture medium was examined. Medium optimization for the mass production of B. subtilis IB101 was performed by using Plackett-Burman design and one factor at a time method. The removal of ammonia gas was more efficient at exponential phase by 20% than at stationary phase. The ammonia gas removal was the highest at pH 4 and 30 $^{\circ}C$. There was not any significant influence of concentration of $(NH_4)_2SO_4$ on the removal of ammonia gas. The components of optimized medium for the production of viable Bacillus subtilis IB101 was yeast extract 10 g/l, soluble starch 2.5 g/l, $MgSO_4$ 6 g/l, $CaCl_2$ 1.55 g/l, $(NH_4)_2SO_4$ 5 g/l, $KH_2PO_4$ 0.75 g/l, soy bean meal 8 g/l.
Keywords
Ammonia gas; Bacillius subtilis IB101;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Seo, M. C., K. Kuroda, D. Hanajima, and K. Haga (1998), Effect of thermophilic ammonium tolerant bacteria of mel-odors emission of composting of pig manure, J. Kor. Soc. Soil Sci. fert. 31(1), 77-84
2 htt://www/.cheric.org/ippage/p/ipdata/2000/14/%BD%C7%C7%E8%B8% B9%B9%FD.html
3 Cho, S. I, M. L. Kim, Y. Y. Kim, and W. Y. Yeo (2003), Development of biofilter system for ammonia removal in livestock facility, J. Kor. Soc, Agr, Mach. 28(5), 457-464
4 Korean Environment Policy Bulletin (2005), Ministry of Environment
5 Park, J. H., G. I. Jun, and C. H . Jeong (2003), Ammonia removal characteristics by pyroligneous liquid at livestock farmhouse, J. Environ. Sci. 12, 1309-1313   과학기술학회마을   DOI
6 http://www.koreapork.or.kr/JOURNAL/9911-monty/new/new3.html
7 Koo, J. H, I. J. Chio, H S. Nam, H. J. Lee, Z. I. Shin, and T. K. Oh (1997), Medium optimization for production of thermostable alkaline proteases from Bacillus licheniformis NS70, Kor, J. Appl. Microbiol. Biotechnol, 25(2), 207-211   과학기술학회마을
8 ee, S. J., D. I. Chang, and H. H. Chang (2006), Effect of biofilter made of composted pine tree bark and perite on reducing odor from pig house, Kor, J. Environ. Agr. 25(2), 118-123   과학기술학회마을   DOI
9 Park, Y. G. (2004), Study of ammonia-gas removal's characteristics using wood-material, Kor. J. San, 19(2), 7-14   과학기술학회마을
10 Lee, S. H., S. B. Lee, H. G. Lee, and S. Y. Oh (2001), Experimental design methodology for quality improvement, Proceedings of Spring Symposium of Korean Operation Research and Management Science Society and Korean Institute of Industrial Engineers
11 Lee, S. J., S. W. Chio, H. Namkung, and I. K. Paik (2000), Effect of dietary protein and feed additive on ammonia gas emission in broiler house, J. Anim, Sci, Technol, 42(3), 299-314
12 Kang, K. H., S. K. Kim, C. G. Hu, and M. G. Lee (2006), The effect of reduction of contaminants and odor according to the additives in the anaerobic maturation process of plggery slurry, J. Environ, Sci, 15(2), 169-175
13 Kim, N. J., Y. Sugano, M. Hirai, and M. Shoda (2000), Removal of a high load of ammonia gas by a marine bacterium, Vibrio alginolyticus, J. Biosci, Bioeng, 90(4), 410-415   DOI
14 Kim, N. J. and Y. C. Jung (2002), Study on the treatment of ammonia gas at high loading by a new bacteria, Vibrio alginolyticus, Proceedings of Spring Joint Syrnposlum of Korean Society on water Quality and Korean Society of water and Wastewater, 363-366