• 한국잡초학회지
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• 제15권2호
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• pp.131-140
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• 1995

본 실험은 allelopathic물질을 함유한 것으로 알려진 alfalfa식물을 이용하여 주요 특용작물과 잡초의 발아 및 생육에 어떠한 영향을 미치는가에 대한 특성을 조사하기 위하여 실시하였던바 그 결과는 다음과 같다. 1. Alfalfa 건조분말의 토양혼화시 건조분말의 농도가 높아짐에 따라 작물, 들깨, 도라지 및 잡초(바랭이, 강아지 풀, 털진득찰, 개비름, 까마중)의 발아율 및 줄기와 뿌리생육이 억제되었으며, 건조분말농도가 10% 이상일때는 80% 이상 발아 및 생육의 억제를 가져왔다. 2. Alfalfa의 생체추출물도 작물(알팔파, 보리, 호밀, 참깨) 및 잡초(피, 강아지풀, 쇠비름)의 발아 및 뿌리와 줄기생육을 억제하였으며 억제정도는 생체추출 농도, 작물 및 잡초의 종류에 따라 반응의 차이를 보였다. 일반적으로 생체추출물의 농도가 증가함에 따라 잡초 및 작물발아 및 뿌리와 줄기생육을 억제하는 정도가 크게 나타났다. 3. 알팔파 건조분말 추출물질도 알팔파, 도라지, 보리, 참깨 및 잡초(바랭이, 털진득찰, 강아지풀, 쇠비름, 피) 등의 발아율, 줄기 및 뿌리의 생육을 억제하였으며 억제정도는 추출농도, 작물 및 잡초의 종류에 따라 차이를 보였다. 건조분말 농도가 10% 이상이었을때 털전득찰, 쇠비름 등의 발아가 전혀 안되었으며 강아지폴은 15%만 발아가 되었다. 4. Gas chromatography에 의하여 분석한 알팔파가 함유한 phenol compounds 중 salicylic acid가 전체 phenol compounds의 70% 정도 차지하고 있어서 가장 많이 함유하고 있었으며 syringic acid, ferulic acid가 가장 적게 함유되어 있었다.

• 한국식품조리과학회지
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• 제14권1호
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• pp.106-113
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• 1998

The purpose of this study is to investigate the antimicrobial effect of mugwort (Artemisia asiatica Nakai) on the rice cake and bread preservation, and to identify their antimicrobial compounds. The mugwort extracts showed complete inhibition on the growth of Bacillus subtilis and Staphylococcus aureus at 250 $\mu\textrm{g}$/ml level. Antimicrobial activi쇼 of mugwort extract were stronger than that of commercial antimicrobial agent. Five % of sodium propionate solution showed complete inhibition on the growth of B. subtilis, E. coli and S. aureus, but L. plantarum was inhibited 50.87% at the same concentration. When various amounts of freeze-dried mugwort powder were added in sulgis (steamed rice cake), 3% ssooksulgi (mugwort powder added sulgi) had quite lower level of total bacterial count (5.5$\times$$10^/5 CFU/g) compared with the control group (1.4$\times$$10^/7 CFU/g) at ambient temp. (30$\pm$1$^{\circ}C$) after 72 hr. Three % addition of mugwort showed 2 days extention of shelf-life of rice cake. The sensory qualities of ssooksulgi has no significant difference in moistness, consistency, cohesiveness, afterswallowing and overall quality compared with control group. Ssooksulgi with 3% of mugwort powder had the best overall quality in sensory test. The methanol extract of 500 $\mu\textrm{g}$/ml of mugwort could lead the successful retardation of the growth of putrefactive microorganism during the incubation of rice cake at 37$^{\circ}C$ for 24 hr. On the other hand, coumarin (Sigma) had 54% inhibitory effect at 500 $\mu\textrm{g}$/ml level, and (E,E)-2,4-decadienal completely inhibited the growth of putrefactive microorganism of whitesulgi at 100 $\mu\textrm{g}$/ml level during the incubation at 37$^{\circ}C$ for 48 hr.48 hr.

• 한국식품조리과학회지
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• 제28권2호
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• pp.175-182
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• 2012

In this study, antibacterial activity and cytotoxicity of the extracts from pericarp and seed of $Vitis$ $coignetiea$, which were extracted with 0.1% HCl-60% ethanol, were analyzed. The antibacterial activity of the extracts was determined by paper disc diffusion method against food spoilage and food-borne pathogens. The pericarp extract showed high antibacterial activity against $Bacillus$ $cereus$, $Escherichia$ $coli$ O157:H7, and $Pseudomonas$ $aeruginosa$, and the seed extract represented the high antibacterial activity against $B.$ $cereus$, $E.$ $coli$ O157:H7, and $Staphylococcus$ $aureus$. The cytotoxicity of the $Vitis$ $coignetiea$ extract against human cancer cells was determined using the MTT assay and SRB assay. The pericarp extract represented strong growth-inhibition activity against G361 and Hep3B cells and the seed extract greatly inhibited the growth of HeLa and G361 cells in the MTT assay. In addition, the pericarp extract displayed a high inhibition activity against the growth of AGS cells and the seed extract greatly inhibited the growth of HeLa, Hep3B, and MCF7 cells in the SRB assay. Especially, the cytotoxicities of the seed extract against HeLa were significantly higher than those of the extract against other cancer cells at all test concentrations. This study demonstrates that the extract from pericarp and seed of $Vitis$ $coignetiea$ possess high antibacterial activity and cytotoxicity.

• 한국자원식물학회지
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• 제31권6호
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• pp.695-703
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• 2018

Previously, we have reported a plant extract isolated from Lysimachia foenum gracum Herba as a new environment friendly biopesticide that has the mycelial growth inhibition effect on Magnaporthe oryzae, the pathogenic fungus of the rice blast disease. For the finding of additional biopesticide candidate, we tested the mycelial growth inhibitory effects about 700 species of plant extracts on PDA media. Among them, the extract of Anemarrhena asphodeloides showed prominent inhibitory effect of which $IC_{50}$ was $139.7{\mu}g/ml$. Mycelial radii of M. oryzae were measured on PDA medium containing the four organic solvent fractions isolated from total extract from A. asphodeloides. Ethyl acetate fraction showed the impressive inhibitory effect of $IC_{50}$, $54.12{\mu}g/ml$. In the subsequent rice field test for the total extract of A. asphodeloides, we obtained encouraging 62.0% control rate of rice blast disease without any phytotoxicity. It is almost equivalent to that of chemical pesticides implying the applicability of the extract as a new biopesticide. In further study, the analysis of active ingredients of the extract would be necessary for the development of a new biopesticide and for the verification of cellular mechanism by which the mycelial growth of M. oryzae inhibited.

• 한국식품영양과학회지
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• 제27권4호
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• pp.625-632
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• 1998

To enhance the antimutagenic and anticancer activities of chinese cabbage kimchi, 13 kinds of kimchi, which were different kinds and levels of sub-ingredient added kimchi, were prepared and fermented at 15℃ for 1 day and then at 5℃ up to pH 4.3. The antimutagenic effects of the methanol extracts of the kimchi were studied by using Ames mutagenicity test in Salmonella typhimurium TA100 and SOS chromotest in E. coli PQ37. Among the kimchi samples, high ratio of red pepper powder(7%) and garlic(2.8% or 5.2%) added kimchi, 1% chinese pepper powder added kimchi and organic cultivated chinese cabbage kimchi significantly reduced(p<0.05) the mutagenicity induced by aflatoxin B1(AFB1) in Ames test and SOS response against N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in SOS chromotest, and also the kimchi inhibited more effectively(p<0.05) the survival and growth of AGS human gastric adenocarcinoma cells than the standarized kimchi on the SRB assay, MTT assay and growth inhibition test. These results suggest that the antimutagenic and anticancer activites of kimchi can be increased by the sub-ingredients such as organic cultivated chinese cabbage, red pepper powder, garlic and chinese pepper powder.

• 동의생리병리학회지
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• 제18권2호
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• pp.561-570
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• 2004

To screen the effective materials for hair loss treatment, the Gamissanghwa-tang extracts were tested. As a result we found that the Gamissanghwa-tang extracts have the hair growth promoting effect. After topical application of each test materials to the back of CS7BL/6 mice, the earlier conversion of telogen-to-anagen phase was induced. In the experiments of 5α-reductase type II inhibition assay, Radix Paeoniae Alba, Semen Cuscutae showed effective potential to inhibit the activity of 5α-reductase type II. And hair growth index of the Gamissanghwa-tang extracts ranked as 1.2, especially the hair growth index of Fructus Rubi is highest as 1.8. But there were no plant extracts which have effect on the DNA proliferation of hair dermal papilla cell measured by [³H]thymidine incorporation, the expression of growth factors such as IGF-I, KGF, HGF estimated by RT-PCR and protein synthesis of vibrissae hair follicle measured by [/sup 35/S] cysteine incorporation. Cortex Cinnamomi showed anti-bacterial effect on P. ovale, Radix Paeoniae Alba has the highest radical scavening activity and Radix Glycyrrhizae has the highest effects of NO synthesis. These results suggest that Gamissanghwa-tang can be used as a potent treatment agent for helping hair growth stimulation.

• 한국토양비료학회지
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• 제34권6호
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• pp.394-400
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• 2001

가축분 및 음식물 퇴비 132점에서 517균주를 분리하고 CMC와 xylan 및 PGA 배지에서 강한 활성을 가지는 10여개의 균주를 선별하여 배추종자 발아를 이용한 작물 inhibition test와 오이 육묘실험으로 growth test를 거쳐 생육이 가장 양호한 LYH201 균주를 야생균주로 선발하였다. Bergey's manual 방법으로 특성을 조사한 결과 LYH201균주는 Gram 양성균으로 내생포자 형성, oxidase, CMCCase, xylanase, catalase, V-P test, methyl red test, arabinose 등의 탄소원 이용성은 양성이었으며, indole을 생성하고, urease, lecithinase, dihydrolase test는 음성이었고, 전자현미경으로 관찰한 결과 길이가 $2.5{\sim}3.0{\mu}m$, 너비가 $0.5{\sim}0.7{\mu}m$의 간균인 Bacillus subtilis로 확인되었다. Bacillus subtilis LYH201 균주를 MMY를 이용하여 배양한 결과 온도 $50^{\circ}C$, pH 6.0에서 생육이 가장 좋았다.

• 대한약침학회지
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• 제27권2호
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• pp.154-161
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• 2024

Objectives: The objective of this study was to assess the genotoxicity of a no-pain pharmacopuncture (NPP) extract developed in 2022 using a bacterial reverse mutation assay, aiming to further substantiate the safety profile of NPP. Methods: The genotoxicity evaluation involved a bacterial reverse mutation assay to assess the mutagenic potential of NPP extracts with and without metabolic activation. Histidine-requiring Salmonella typhimurium strains (TA98, TA100, TA1535, and TA1537) and tryptophan-requiring Escherichia coli strains (WP2uvrA) were used in the assay. Results: The NPP extract did not induce a revertant colony count exceeding two times that of the negative control at any dose level in any of the tested strains, both with and without metabolic activation. Additionally, no growth inhibition or precipitation was observed in the presence of NPP. Conclusion: Based on the findings, it can be concluded that the NPP extract exhibited no mutagenic potential in the in vitro genotoxicity tests conducted.

• 대한수의학회지
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• 제40권2호
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• pp.325-332
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• 2000

The growth characteristics and the cellular protein patterns of the Lactobacillus plantarum isolated and identified from oat silage were examined in order to confirm whether it will be used practically as probiotics or not. L plantarum was identified by morphological and biochemical tests including of final conforming by API 50CHL kit. The cultivation in MRS broth of the strain under the condition of different temperature, proved that they grew into $2.0{\times}10^{9}$ in $25^{\circ}C$, into $1.4{\times}10^{9}$ in $35^{\circ}C$ but they decreased into $4.5{\times}10^{5}$ growth in $45^{\circ}C$. The comparison of the growth by measurement of O.D600nm value after 24 hour cultivation between L plantarum and commercial probiotics, showed that the strain had a higher growth than commercial as 1.841 : 1.623. The measurement of it under bile acid's existence, indicated that this isolation was not influenced by bile acid and the tolerance was $3.2{\times}10^{9}$, $3.9{\times}10^{9}$ and $3.2{\times}10^{9}$, respectively, when each of 0%, 1%, and 2% oxigall existed. The examination of their antibiotics susceptibility by disk diffusion test, proved that L plantarum showed resistance against danofloxacin(5mcg), gentamycin(10mcg), kanamycin(30mcg), neomycin(30mcg) and streptomycin(10mcg). Based upon the test of the bacteriocin formation of this L plantarum, it was found out that the inhibition zone was not formed. In growth of L plantarum and E coli in nutrient broth, all E coli died out within 6 hours after cultures.

• 농약과학회지
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• 제12권3호
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• pp.222-228
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• 2008

벼재배용 농약의 담수 조류에 대한 독성과 감수성을 조사하고자 4종의 담수 조류를 이용하여 생장저해시험을 실시하였다. 시험농약은 벼재배용으로 사용되는 butachlor 5% GR, butachlor 33% EW, isoprothiolane 12% GR, isoprothiolane 40% EC, diazinon 3% GR, diazinon 34% EC이며, 시험농약을 담수 녹조류 3종(Selenastrum capricornutum, Scenedesmus subspicatus, Chlorella vulgris)과 규조류 1종(Nitzschia palea)에 72시간 노출시켜 그 생장률을 조사하였고 생장저해율을 이용하여 50% 영향농도($EC_{50}$)를 산출하였다. 시험 결과, 모든 시험농약에서 유제와 유탁제가 입제보다 담수 조류에 대한 독성이 높았으며, 특히 유제의 경우 농약 원제보다도 높은 독성이 나타났다. 이러한 결과로 농약 제제의 특성에 따라 수조류에 대한 독성이 높아지거나 낮아지고 있음을 알 수 있었다. 그리고 시험종간 감수성에 있어서는 대부분의 시험농약에서 녹조류인 S. capricornutum와 S. subspicatus는 비슷한 수준의 감수성을 나타내고 있는 반면 C. vulgris의 감수성은 확연히 낮았다. 규조류인 N. palea의 농약에 대한 감수성은 S. capricornutum와 S. subspicatus에 비해 다소 낮거나 비슷하였으나, C. vulgris에 비해 감수성이 높게 나타났다.